| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP000730 | 1,388,143 | T→C | 16.1% | L10L (CTT→CTC) | parC → | DNA topoisomerase (ATP‑hydrolyzing) ParC |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP000730 | 1,388,143 | 0 | T | C | 16.1% | 46.0 / 4.1 | 25 | L10L (CTT→CTC) | parC | DNA topoisomerase (ATP‑hydrolyzing) ParC |
| Reads supporting (aligned to +/- strand): ref base T (14/7); new base C (2/2); total (16/9) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.02e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.83e-01 | |||||||||||
GAATGGATTGAAAAGCATGTTGAGTTTGGTATGCAAGAGGACCAAAGTATTTTAGATAATTCTGAAGTACAAGTGCTTGAAAATGATCAATTTGATGAGGAGGAAATCTAGTGAGTGAAATAATTCAAGATTTATCACTTGAAGATGTTTTAGGTGATCGCTTTGGAAGATATAGTAAATATATTATTCAAGAGCGTGCATTGCCAGATGTTCGTGATGGTTTAAAACCAGTACAACGTCGTATTTTATATGCAATGTATTCAAGTGGTAATACACACGA > CP000730/1388004‑1388283 | gAATGGATTGAAAAGCATGTTGAGTTTGGTATGGAAGAGGACCAAAGTATTTTAGATAATTCTGAAGTACAAGTGCTTGAAAATGATCAATTTGATGAGGAGGAAATCTAGTGAGTGAAATAATTCAAGATTTATCACTTg < 2:65264/141‑1 (MQ=255) ttGAAAAGCATGTTGAGTTTGGTATGCAAGAGGACCAAAGTATTTTAGATAATTCTGAAGTACAAGTGCTTGAAAATGATCAATTTGATGAGGAGGAAATCTAGTGAGTGAAATAATTCAAGATTTATCACTTGAAGATGt > 2:48236/1‑141 (MQ=255) tATGCAAGTGGACCTAAGTATTTTAGATAATTTTGAGGTATAAGTGCTTGAAAATGATCAATTTGATGAGGAGGAGATCTAGTGAGTGAAATAATTCAAGATTTATCACTTGAAGATGTTTTAGGTGATCGCTTTGGAAGa < 1:113303/141‑1 (MQ=255) tATGCAAGAGGACCAAAGTATTTTAGATAATTCTGAAGTACAAGTGCTTGAAAATGATCAATTTGATGAGGAGGAAATCTAGTGAGTGAAATAATTCAAGATTTATCACTCGAAGATGTTTTAGGTGATCGCTTTGGa > 2:79582/1‑138 (MQ=255) tATGCAAGAGGACCAAAGTATTTTAGATAATTCTGAAGTACAAGTGCTTGAAAATGATCAATTTGATGAGGAGGAAATCTAGTGAGTGAAATAATTCAAGATTTATCACTCGAAGATGTTTTAGGTGATCGCTTTGGa < 1:43215/138‑1 (MQ=255) tATGCAAGAGGACCAAAGTATTTTAGATAATTCTGAAGTACAAGTGCTTGAAAATGATCAATTTGATGAGGAGGAAATCTAGTGAGTGAAATAATTCAAGATTTATCACTCGAAGATGTTTTAGGTGATCGCTTTGGa < 1:79582/138‑1 (MQ=255) tATGCAAGAGGACCAAAGTATTTTAGATAATTCTGAAGTACAAGTGCTTGAAAATGATCAATTTGATGAGGAGGAAATCTAGTGAGTGAAATAATTCAAGATTTATCACTCGAAGATGTTTTAGGTGATCGCTTTGGa > 2:43215/1‑138 (MQ=255) cAAGAGGACCAAAGTATTTTAGATAATTCTGAAGTACAAGTGCTTGAAAATGATCAATTTGATGAGGAGGAAATCTAGTGAGTGAAATAATTCAAGATTTATCACTTGAAGATGTTTTAGGTGATCGCTTTGGAAGAtata > 1:165692/1‑141 (MQ=255) ttAGATAATTCTGAAGTACAAGTGCTTGAAAATGATCAATTTGATGAGGAGGAAATCTAGTGAGTGAAATAATTCAAGATTTATCACTTGAAGATGTTTTAGGTGATCGCTTTGGAAGATATAGTAAATATATTATTCAag > 2:90643/1‑141 (MQ=255) tAGATAATTCTGAAGTACAAGTGCTTGAAAATGATCAATTTGATGAGGAGGAAATCTAGTGAGTGAAATAATTCAAGATTTATCACTTGAAGATGTTTTAGGTGATCGCTTTGGAAGATATAGTAAATATATTATTCAaga > 1:125929/1‑141 (MQ=255) gATAATTCTGAAGTAAAAGTGCTTGGAAATGATCAATTTGATGAGGAGGAAATCTAGTGAGTGAAATAATTCAAGATTTATCACTTGAAGATGTTTTAGGTGATCGCTTTGGAAGATATAGTAAATATATTATTCAagagg > 2:229885/1‑140 (MQ=255) tCTGAAGTACAAGTGCTTGAAAATGATCAATTTGATGAGGAGGAAATCTAGTGAGTGAAATAATTCAAGATTTATCACTTGAAGATGTTTTAGGTGATCGCTTTGGAAGATATAGTAAATATATTATTCAAGAGCGTGCAt > 1:32596/1‑141 (MQ=255) gAAGTACAATTGCTTTAAAATGATCAATTTGATGAGGAGGAAAACTAGTGAGTGAAATAATTCAAGATTTATCACTTGAAGATGTTTTAGGTGATCGCCTTGGAAGATATAGTAAATATATTATTCAAGAGCGTGCATTGc < 2:220635/141‑1 (MQ=255) aaGTGCTTGAAAATGATCAATTTGATGAGGAGGAAATCTAGTGAGTGAAATAATTCAAGATTTATCACTTGAAGATGTTTTAGGTGATCGCTTTGGAAGATATAGTAAATATATTATTCAAGAGCGTGCATTGCCAGATGt > 2:23409/1‑141 (MQ=255) tttGATGAGGCGGAAATCTAGTGAGTGAAATAACTCCAGATTTATCACGTGAAGATGTTTTAGGTGATCGCTTTGGAAGATATTGTAAATATATTATTCAAGAGCGTGCATTGCCAGATGTTCGTGATGGTTTAAAACCAg < 1:236458/141‑1 (MQ=255) tgaGGAGGAAATCTAGTGAGGGAAATAATTCAAGTTTTGTCTCGTTAGGATGTGTGAGGTGATCGCTTTGGAAGATATAGTAAATATATTATTCAAGAGCGTGCATTGCCAGATGTTCGTGATGGTTTAAAACCAGTACaa > 2:281444/1‑141 (MQ=38) aggaAATCTAGTGAGTGAAATAATTCAAGATTTATCACTTGAAGATGTTTTAGGTGATCGCTTTGGAAGATATAGTAAATATATTATTCAAGAGCGTGCATTGCCAGATGTTCGTGATGGTTTAAAACCAGTACAAcgtcg < 1:90643/141‑1 (MQ=255) aTCTAGTGAGTGAAATAATTCAAGATTTATCACTTGAAGATGTTTTAGGTGATCGCTTTGGAAGATATAGTAAATATATGATTCATGCGAATGCAGTGCCAGATGTTCGTGATGGGGTGAAAACAGTACAACGTCGTAttt > 1:250244/1‑141 (MQ=255) cTAGTGAGTGAAATAATTCAAGATTTATCACTTGAAGATGTTTTAGGTGATCGCTTTGGAAGATATAGTAAATATATTATTCAAGAGCGTGCATTGCCAGATGTTCGTGATGGTTTAAAACCAGTACAACGTCGTATTTta > 1:19121/1‑141 (MQ=255) aaaTAATTCAAGATTTATCACTTGAAGATGTTTTAGGTGATCGCTTTGGAAGATATAGTAAATATATTATTCAAGAGCGTGCATTGCCAGATGTTCGTGATGGTTTAAAACCAGTACAACGTCGTATTTTATATGCAATGt > 1:32906/1‑141 (MQ=255) aaaTAATTCAAGATTTATCACTTGAAGATGTTTTAGGTGATCGCTTTGGAAGATATAGTAAATATATTATTCAAGAGCGTGCATTGCCAGATGTTCGTGATGGTTTAAAACCAGTACAACGTCGTATTTTATATGCAATGt > 1:154868/1‑141 (MQ=255) aaGATTTATCACTTTAAGATGTTTTAGGTGATCGCTTTGGAAGATATAGTAAATATATTATTCAAGAGCGTGCATTGCCAGATGTTCGTGATGGTTTAAAACCAGTACAACGTCGTATTTTATATGCAATGTATTCAAGTg < 2:10489/141‑1 (MQ=255) aaGATTTATCACTTGAAGATGTTTTAGGTGATCGCTTTGGAAGATATAGTAAATATATTATTCAAGAGCGTGCATTGCCAGATGTTCGTGATGGTTTAAAACCAGTACAACGTCGTATTTTATATGCAATGTATTCAAGTg < 1:98794/141‑1 (MQ=255) aGATTTATCACTTGAAGATGTTTTAGGTGATCGCTGTGGAAGATATAGTAAATATATTATTCAAGAGCGTGCATTGCCAGATGTTCGTGATGGTTTAAAACCAGTACAACGTCGTATTTTATATGCAATGTATTCAAGtag > 1:237815/1‑139 (MQ=255) ttATCACTTGAAGATGTTTTAGGTGATCGCTTTGGAAGATATAGTAAATATATTATTCAAGAGCGTGCATTGCCAGATGTTCGTGATGGTTTAAAACCAGTACAACGTCGTATTTTATATGCAATGTATTCAAGTGGTAAt > 1:230355/1‑141 (MQ=255) tGAAGATGTTTAGGGTGATCGCTTTGGAAGATATAGTAAATATATTATGAAAGGGCGTGCATTGCCAGATGTTCGTGATGGTTTAAAACCAGTACAATGTCGTATTTTATATGCAATGTATTCAAGTGGTAATACACACGa < 1:107961/141‑1 (MQ=38) | GAATGGATTGAAAAGCATGTTGAGTTTGGTATGCAAGAGGACCAAAGTATTTTAGATAATTCTGAAGTACAAGTGCTTGAAAATGATCAATTTGATGAGGAGGAAATCTAGTGAGTGAAATAATTCAAGATTTATCACTTGAAGATGTTTTAGGTGATCGCTTTGGAAGATATAGTAAATATATTATTCAAGAGCGTGCATTGCCAGATGTTCGTGATGGTTTAAAACCAGTACAACGTCGTATTTTATATGCAATGTATTCAAGTGGTAATACACACGA > CP000730/1388004‑1388283 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |