| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP000730 | 1,474,639 | C→G | 100% | A9129P (GCA→CCA) | ebh ← | extracellular matrix binding protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP000730 | 1,474,639 | 0 | C | G | 100.0% | 55.7 / NA | 19 | A9129P (GCA→CCA) | ebh | extracellular matrix binding protein |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base G (14/5); total (14/5) | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
CCTTTAAATCCATCTTGTTGAGCATTGTTTAATGCATTCAATTGGTCGATGTCATGTTTACCATTTGTTTTAGCAAGTGCTACACGTTCATCACCATTTAATGCTTGTTTTGCATTTAAAATAT‑TTTGAAGTGCTTGATCCACTTGTTGCTTATTCGCATTTTGTTTCAATACATTTTCAGCATCAGCAATAGCTTGTTTATAAGCATTTTGTTTATCTGAATCTGCTTGTGTGTAATCTACAGTTTGCTCAACGTTAGCATGTTCATTA > CP000730/1474506‑1474775 | ccTTTAAATCCATCTTGTTGAGCATTGTTTAATGCATTCAATTGGTCGATGTCATGTTTACCATTTGTTTTAGCAAGTGCTACACGTTCATCACCATTTAATGCTTGTTTTGCATTTAAAATAT‑TTTGAAGTGGTTGATcc > 1:255673/1‑141 (MQ=255) gttgAGCATTGTTTAATGCATTCAATTGGTCGATGTCATGTTTACCATTTGTTTTAGCAAGTGCTACACGTTCATCACCATTTAATGCTTGTTTTGCATTTAAAATAT‑TTTGAAGTGGTTGATCCACTTGTTGCTTATTCg > 1:101080/1‑141 (MQ=255) gCATTGTTTAATGCATTCAATTGGTCGATGTCATGTTTACCATTTGTTTTAGCAAGTGCTACACGTTCATCACCATTTAATGCTTGTTTTGCATTTACAATAT‑TTTGAAGTGGTTGATCCACTTGTTGCTTATTCGCAttt > 2:140295/1‑141 (MQ=255) cATTGTTTAATGCATTCAATTGGTCGATGTCATGTTTACCATTTGTTTTAGCAAGTGCTACACGTTCATCACCATTTAATGCTTGTTTTGCATTTAAAATAT‑TTTGAAGTGGTTGATCCACTTGTTGCTTATTCGCAtttt > 2:64561/1‑141 (MQ=255) tGTTTAATGTATTCAATTGGTCGATGTCATGTTTACCATTTGTTTTAGCAAGTGCTACACGTTCATCACCATTTAATGCTTGTTTTGCATTTAAAATAT‑TTTGAAGTGGTTGATCCACTTGTTGCTTATTCGCATTTTGtt > 1:208193/1‑141 (MQ=255) aTTCAATTGGTCGATGTCATGTTTACCATTTGTTTTAGCAAGTGCTACACGTTCATCACCATTTAATGCTTGTTTTGCATTTAAAATAT‑TTTGAAGTGGTTGATCCACTTGTTGCTTATTCGCATTTTGTTTCAATACAtt > 2:235728/1‑141 (MQ=255) ttaaaTTGGTAGAGGTCATTTTTACCATTTGTTTTAGCAAGTGCTACACGTTCATCACCATTTAATGCTTGTTTTGCATTTAAAATAT‑TTTGAAGTGGTTGATCCACTTGTTGCTTATTCGCATTTTGTTTCAATACAttt < 2:217795/138‑1 (MQ=255) tGGTCGATGTCATGTTTACCATTTGTTTTAGCAAGGACTACGCGTGTACTACCACTTAATGCCTGTTTTTCCTTTAAAATAT‑TTGGAAGTGGTTGATCCACTTGTTGCTTATTCTCATTTTGTTTCCATACATTTtcagca < 2:101184/141‑1 (MQ=255) gTCGATGTCATGTTTACCATTTGTTTTAGCAAGTGCTACACGTTCATCACCATTTAATGCTTGTTTTGCATTTAAAATAT‑TTTGAAGTGGTTGATCCACTTGTTGCTTATTCGCATTTTGTTTCAATACATTTtcagcatc > 1:215048/1‑141 (MQ=255) gTCGATGTCATGTTTACCATTTGTTTTAGCAAGTGCTACACGTTCATCACCACATAATGCGTGTTTTGCATTTAAAACCTATGTGAGACGGTTG‑GGCACTTGTTTCTTATTCGCATTTTGTTTCAATACATTTtcatcatc > 2:272665/1‑141 (MQ=255) atccaGTGGTACACGTTCATCACCAATGAATTCTTTTTTTTAATTTAAAATCT‑TTTGAAGTGGTTGATCCACTTGTTGCTTATTCGCATTTTGTTTCAATACATTTTCAGCATCAGCAATAGCTTGTTTATAAGCATTTTg < 1:258969/137‑1 (MQ=255) gCAAGTGCTACACGTTCATCACCATTTAATGCTTGTTTTGCATTTAAAATAT‑TTTGAAGTGGTTGATCCACTTGTTGCTTATTCGCATTTTGTTTCATTACATTTTTAGCATCAGCAATAGCTTGTTTATAAGCAATTTGt > 2:254861/1‑141 (MQ=255) cGTTCGTCACCATTTAATGCTTGTTTTGCATTTAAAATAT‑TTTGAAGTGGTTGATCCACTTGTTGCTTATTCGCATTTTGTTTCAATACATTTTCAGCATCAGCAATAGCTTGTTTATAAGCATTTTGTTTATCTGAATCt > 1:145145/1‑141 (MQ=255) ttCATCACCATTTAATGCTTGTTTTGCATTTAAAATAT‑TTTGAAGTGGTTGATCCACTTGTTGCTTATTCGCATTTTGTTTCAATACATTTTCAGCATCAGCAATAGCTTGTTTATAAGCATTTTGTTTATCTGAATCTGc > 2:153272/1‑141 (MQ=255) ttAATGCTTGTTTTGCATTTAAAATAT‑TTTGAAGTGGTTGATCCACTTGTTGCTTATTCGCATTTTGTTTCAATACATTTTCAGCATCAGCAATAGCTTGTTTATAAGCATTTTGTTTATCTGAATCTGCTTGTGTGTAAt < 1:64561/141‑1 (MQ=255) cTTGTTTTGCATTTAAAATAT‑TTTGAAGTGGTTGATCCACTTGTTGCTTATTCGCATTTTGTTTCAATACATTTTCAGCATCAGCAATAGCTTGTTTATAAGCATTTTGTTTATCTGAATCTGCTTGTGTGTAATCTACAg > 2:144300/1‑141 (MQ=255) at‑TTTGAAGTGGTTGATCCACTGGTTGCTTGTTCGCATTTTGTTTCAATACATTTTCAGCATCAGCAATAGCTTGTTTATAAGCATTTTGTTTATCTGAATCTGCTTGTGTGTAATCTACAGTTTGCTCAACGTTAGCATg > 1:220534/1‑141 (MQ=255) ttGAGGTGGTTGTTCCACTTGTTGCTTATTCGCATTTTGTTTCAATACATTTTCAGCATCAGCAATAGCTTGTTTATAAGCATTTTGTTTCTCTGATTCTGCGTGTGTGTAGTCTACAGTTTGCTCACCGTTAGCATGTTc < 2:273274/141‑1 (MQ=255) aGTGGTTGATCCACTTGTTGCTTATTCGCATTTTGTTTCAATACATTTTCAGCATCAGCAATAGCTTGTTTATAAGCATTTTGTTTATCTGAATCTGCTTGTGTGTAATCGTCAGTTTGCTCACCGTTAGCATGTTCAttt > 2:179926/1‑140 (MQ=255) | CCTTTAAATCCATCTTGTTGAGCATTGTTTAATGCATTCAATTGGTCGATGTCATGTTTACCATTTGTTTTAGCAAGTGCTACACGTTCATCACCATTTAATGCTTGTTTTGCATTTAAAATAT‑TTTGAAGTGCTTGATCCACTTGTTGCTTATTCGCATTTTGTTTCAATACATTTTCAGCATCAGCAATAGCTTGTTTATAAGCATTTTGTTTATCTGAATCTGCTTGTGTGTAATCTACAGTTTGCTCAACGTTAGCATGTTCATTA > CP000730/1474506‑1474775 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |