Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 2,028,233 G→C 19.0% Q138E (CAA→GAA)  USA300HOU_1882 ← hypothetical membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007302,028,2330GC19.0% 55.5 / 7.4 27Q138E (CAA→GAA) USA300HOU_1882hypothetical membrane protein
Reads supporting (aligned to +/- strand):  ref base G (10/11);  new base C (2/3);  total (13/14)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.46e-01

AGCTACATGTAAATTAATATTGTAATTAACCGGCAATATAACCTGCACTTTACCTAAAATGTGTCTAACAACAATGGTATTATTTTCCTTAATATTTGCAGCTTTTGTTAAGTCAATATGTAGGTCGCCAATTCCATGTTGAATTTGTACATCTTCCCACTTATATACATAAACTGGTGTACGTTGCTCACCAAACCACTTTTGTTTAATAAAAGATGGTGAAGTCATAACCTCATCCGTCGCAACTACTTTTTTTGGTTTAAACTTGTGTAT  >  CP000730/2028093‑2028365
                                                                                                                                            |                                                                                                                                    
agcTACATGTAAATTAATATTGTAAGTAACCGGCAATATAACCTGCACTTTACCTAAAATGTGTCTAACAACAATGGTATTATTTTCCTTAATATTTGCAGCTTTTGTTAAGTCAATATGTAGGTCGCCAATTCCATGTTg                                                                                                                                      <  1:607/141‑1 (MQ=255)
           aaTTAATATTGTAATTAACCGGCAATATAACCTGCACTTTACCTAAAATGTGTCTAACAACAATGGTATTATTTTCCTTAATATTTGCAGCTTTTGTTAAGTCAATATGTAGGTCGCCAATTCCATGTTGAATTTGTACAt                                                                                                                           <  1:5150/141‑1 (MQ=255)
           aaTTAATATTGTAATAAACCGGCAATATAACCTGCACTTTACCTAAAATGTGTCTAACAACAATGGTATTATTTTCCTTAATATTTGCAGCTTTTGTTAAGTAAATATGTAGGTCGCCAATTCCATGTTGAATTTGTACAt                                                                                                                           <  1:5208/141‑1 (MQ=255)
            aTTAATATTGTAATTAACCGGCAATATAACCTGCACTTTACCTAAAATGTGTCTAACAACAATGGTATTATTTTCCTTAATATTTGCAGCTTTTGTTAAGTCACTATGTAGGTGGCCAATTCCATTTTTAATTTGTACATc                                                                                                                          >  1:128205/1‑141 (MQ=255)
                atatTGTAATTAACCGGCAATATAACCTGCACTTTACCTAAAATGTGTCTAACAACAATGGTATTATTTTCCTTAATATTTGCAGCTTTTGTTAAGTCAATATGTAGGTCGCCAATTCCATGTTGAATTTGTACATCTTcc                                                                                                                      <  1:26882/141‑1 (MQ=255)
                   ttGTAATTAACCGGCAATATAACCCGCAGATTACCTAAAATGTGTCTAACAACAATGGTATTATTTTCCTTAATATTTGCAGCTTTTGTTAAGTCAATATGTAGGTCGCCAATTACATGTTGAATTTGTACATCTTCacac                                                                                                                   >  1:180410/1‑141 (MQ=255)
                    tGTAATTAACCGGCAATATAACCTGCACTTTACCTAAAATGTGTCTAACAACAATGGTATTATTTTCCTTAATATTTGCAGCTTTTGTTAAGTCAATATGTAGGTCGCCAATTCCATGTTCAATTTGTCCATCTTCCCACt                                                                                                                  >  2:19269/1‑141 (MQ=255)
                                      taACCTGCACTTTACCTAAAATGTGTCTAACAACAATGGTATTATTTTCCTTAATATTTGCAGCTTTTGTTAAGTCAATATGTAGGTCGCCAATTCCATGTTGAATTTGTACATCTTCCCACTTATATACATAAACTGgtg                                                                                                <  1:265984/141‑1 (MQ=255)
                                          cTGCACTTGACCTAAAATGTGTCTAACAACAATGGTATTATTTTCCTTAATGTTTGCACATTTTTTTAAGTCAATATGTAGGTCGCCAATTCCATGTTGAATTTGTTCATCTTCCCACTTATATCCATCAACTGGTGTAct                                                                                            >  2:130290/1‑140 (MQ=255)
                                           tGCACTTTACCTAAAATGTGTCTAACAACAATGGTATTATTTTCCTTAATATTTGCAGCTTTTGTTAAGTCAATATGTAGGTCGCCAATTCCATGTTGAATTTGTACATCTTCCCACTTATATACATAAACTGGTGTACGt                                                                                           <  2:261214/141‑1 (MQ=255)
                                                         aaTGTGTCTAACAACAATGGTATTATTTTCCTTAATATTTGCAGCTTTTGTTAAGTCAATATGTAGGTCGCCAATTCCATGTTGAATTTGTACATCTTCCCACTTATATACATAAACTAGTGTACGGTGCTCACCAAACCa                                                                             <  2:131222/141‑1 (MQ=255)
                                                                        aaTGGTATTGTTTTCCTTAATATTTGCAGCTTTTGTTAAGTCAATATGTAGGTCGCCAATTCCATGTTGAATTTGTACATCTTCCCACTTATATACATAAACTGGTGTACGTTGCTCACCAAACCACTTTTGTTTAATaaa                                                              >  1:208255/1‑141 (MQ=255)
                                                                         aTGGTATTATTTTCCTTAATATTTGCAGCTTTTGTTAAGTCAATATGTAGGTCGCCAATTCCATGTTCAATTTGTACATCTTCCCACTTATATACATAAACTGGTGTACGTTGCTCACCAAACCACTTTTGTTTAATaaaa                                                             <  2:219970/141‑1 (MQ=255)
                                                                              attattTTCCTTAATATTTGCAGCTTTTGTTAAGTCAATATGTAGGTCGCCAATTCCATGTTGAATTTGTACATCTTCCCACTTATATACATAAACTGGTGTACGTTGCTCACCAAACCACTTTTGTTTAATAAAAGATgg                                                        >  1:175148/1‑141 (MQ=255)
                                                                                           atatTTGCAGCTTTTGTTAAGTCAATATGTAGGTCGCCAATTCCATGTTCAATTTGTACATCTTCCCACTTATATACATAAACTGGTGTACGTTGCTCACCAAACCACTTTTGTTTAATAAAAGATGGTGAAGTCATAAcc                                           >  1:120520/1‑141 (MQ=255)
                                                                                            tatTTGCAGCTTTTGTTAAGTCAATATGTAGGTCGCCAATTCCATGTTGAATTTGTACATCTTCCCACTTATATACATAAACTGGTGTACGTTGCTCACCAAACCACTTTTGTTTAATAAAAGATGGTGAAGTCATAACCt                                          <  1:147126/141‑1 (MQ=255)
                                                                                                 gCAGCTTTTGTTAAGTCAATATGTAGGTCGCCAATTCCATGTTGAATTTGTACATCTTCCCACTTATATACATAAACTGGTGTACGTTGCTCACCAAACCACTTTTGTTTAATAAAAGATGGTGAAGTCATAACCTCATcc                                     >  1:182708/1‑141 (MQ=255)
                                                                                                   aGCTTTTGTTAAGTCAATATGTAGGTCGCCAATTCCATGTTGAATTTGTACATCTTCCCACTTATATACATAAACTGGTGTACGTTGCTCACCAAACCACTTTTGTTTAATAAAAGATGGTGAAGTCATAACCTCATCCGt                                   >  2:32256/1‑141 (MQ=255)
                                                                                                        ttGTTAAGTCAATAGGTAGGTCGCCAATTCCATGTTGAATTTGTACATCTTCCCACTTATATACATAAACTGGTTTACGTTGCTCACCAAACCACTTTTGGTTAATAAAAGATGGTGACGTCATAACCTCATCCGTCGCaa                              >  2:107833/1‑141 (MQ=255)
                                                                                                          tttAAGTCAATATGTAGGTCGCCAATTCCAAGTTGAATTTGTACATCTTCCCACTTATATACATAAACTGGTGTACGTTGCTCACCAAACCACTTTTGTTTAATAAAAGATGGTGAAGTCATAACCTCATCCGTCGCAact                            <  2:180410/140‑1 (MQ=255)
                                                                                                            tAAGTCAATATGTAGGTCGCCAATTCCATGTTGAATTTGTACATCTTCCCACTTATATACATAAACTGGTGTACGTTGCTCACCAAACCACTTTTGTTTAATAAAAGATGGTGAAGTCATAACCTCATCCGTCGCAactac                          >  2:126908/1‑141 (MQ=255)
                                                                                                                       gTAGGTCGCCAATTCCATGTTGAATTTGTACATCTTCCCACTTATATACATAAACTGGTGTACGTTGCTCACCAAACCACTTTTGTTTAATAAAAGATGGTGAAGTCATAACCTCATCCGTCGCAACTACTTTTTTTGGtt               <  1:12210/141‑1 (MQ=255)
                                                                                                                       gTAGGTCGCCAATTCCATGTTCAATTTGTACATCTTCCCACTTATATACATAAACTGGTGTACGTTGCTCACCAAACCACTTTTGTTTAATAAAAGATGGTGAAGTCATAACCTCATCCGTCGCAACTACTTTTTTTGGtt               <  2:206267/141‑1 (MQ=255)
                                                                                                                        tAGGTCGCCAATTCCATGTTGAATTTGTACATCTTCCCCCTTATATACATAAACTGGTGTACGTTGCTCACCAAACCACTTTTGTTTAATAAAAGATGGTGAAGTCATAACCTCATCCGTCGCAACTACTTTTTTTGGttt              <  1:273997/141‑1 (MQ=255)
                                                                                                                          ggTCGCCAATTCCATGTTGAATTTGTACATCTTCCCACTTATATACATAAACTGGTGTACGTTGCTCACCAAACCACTTTTGTTTAATAAAAGATGGTGAAGTCATAACCTCATCCGTCGCAACTACTTTTTTTGGTTTaa            >  1:267337/1‑141 (MQ=255)
                                                                                                                          ggTCGCCAATTCCATGTTGAATTTGTACATCTTCCCACTTATATACATAAACTGGTGTACGTTGCTCACCAAACCACTTTTGTTTAATAAAAGATGGTGAAGTCATAACCTCATCCGTCGCAACTACTTTTTTTGGTTTaa            >  1:267319/1‑141 (MQ=255)
                                                                                                                                  aTTCCATGTTGAATTTGTACATCTTCCCACTTATATACATAAACTGGTGTACGTTGCTCACCAAACCACTTTTGTTTAATAAAAGATGGTGAAGTCATAACCTCATCCGTCGCAACTACTTTTTTTGGTTTAAACTTgtgt    <  2:250581/141‑1 (MQ=255)
                                                                                                                                    tCCATGTTCAATTTGTACATCTTCCCACTTATATACATAAACTGGTGTACGTTGCTCACCAAACCACTTTTGTTTAATAAAAGATGGTGAAGTCATAACCTCATCCGTCGCAACTACTTTTTTTGGTTTAAACTTGTGTAt  <  1:19269/141‑1 (MQ=255)
                                                                                                                                            |                                                                                                                                    
AGCTACATGTAAATTAATATTGTAATTAACCGGCAATATAACCTGCACTTTACCTAAAATGTGTCTAACAACAATGGTATTATTTTCCTTAATATTTGCAGCTTTTGTTAAGTCAATATGTAGGTCGCCAATTCCATGTTGAATTTGTACATCTTCCCACTTATATACATAAACTGGTGTACGTTGCTCACCAAACCACTTTTGTTTAATAAAAGATGGTGAAGTCATAACCTCATCCGTCGCAACTACTTTTTTTGGTTTAAACTTGTGTAT  >  CP000730/2028093‑2028365

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑