BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP000730	479,553	G→A	100%	A194T (GCA→ACA)	USA300HOU_0458 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	479,553	0	G	A	100.0%	95.6 / NA	33	A194T (GCA→ACA)	USA300HOU_0458	hypothetical protein
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (10/23); total (10/23)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

TGATGCAATAATAGATGAACAAGGTGAGCCACTTAGCGAACAAGTGAATCGTCAAATGATTAAATGGACGAAACTTTATATCGATCAATTTCTATCGAGTTGGACAATGCCGAAGCGTGAGCAAAGTTTTTACCATGCATGGTTGCATTTAGCGCAACATGACCATAGTTTTACTAAAGCACAGCGCCAAGTGATTAAAGGCTTACCCAATGATCCTGAAATGACGATAGAGTCAGTATTAACTCATTTTTCAATAGATCAGGAAGACTACCA  >  CP000730/479417‑479689

tGATGCAATAATAGATGAACAAGGTGAGCCACTTAGCGAACAAGTGAATCGTCAAATGATTAAATGGACGAAACTTTATATCGATCAATTTCTATCGAGTTGGACAATGCCGAAGCGTGAGCAAAGTTTTTACcatacatg                                                                                                                                      >  2:225410/1‑141 (MQ=255)

        taataGATGAACAAGGTGAGCCACTTAGCGAACAAGTGAATCGTCAAATGATTAAATGGACGAAACTTTATATCGATCAATTTCTATCGAGTTGGACAATGCCGAAGCGTGAGCAAAGTTTTTACCATACATGGTTGCAtt                                                                                                                              <  1:258082/141‑1 (MQ=255)

        taataGATGAACAAGGTGAGCCACTTAGCGAACAAGTGAATCGTCAAATGATTAAATGGACGAAACTTTATATCGATCAATTTCTATCGAGTTGGACAATGCCGAAGCGTGAGCAAAGTTTTTACCATACATGGTTGCAtt                                                                                                                              <  2:251982/141‑1 (MQ=255)

            aGATGAACAAGGTGAGCCACTTAGCGAACAAGTGAATCGTCAAATGATTAAATGGACGAAACTTTATATCGATCAATTTCTATCGAGTTGGACAATGCCGAAGCGTGAGCAAAGTTTTTACCATACATGGTTGCATTTAgc                                                                                                                          >  2:136886/1‑141 (MQ=255)

            aGATGAACAAGGTGAGCCACTTAGCGAACAAGTGAATCGTCAAATGATTAAATGGACGAAACTTTATATCGATCAATTTCTATCGAGTTGGACAATGCCGAAGCGTGAGCAAAGTTTTTACCATACATGGTTGCATTTAgc                                                                                                                          <  1:11564/141‑1 (MQ=255)

                  aCAAGGTGAGCCACTTAGCGAACAAGTGAATCGTCAAATGATTAAATGGACGAAACTTTATATCGATCAATTTCTATCGAGTTGGACAATGCCGAAGCGTGAGCAAAGTTTTTACCATACATGGTTGCATTTAGCGCAACa                                                                                                                    <  2:4322/141‑1 (MQ=255)

                      ggTGAGCCACTTAGCGAACAAGTGAATCGTCAAATGATTAAATGGACGAAACTTTATATCGATCAATTTCTATCGAGTTGGACAATGCCGAAGCGTGAGCAAAGTTTTTACCATACATGGTTGCATTTAGCGCAACATGAc                                                                                                                >  2:39589/1‑141 (MQ=255)

                             cACTTAGCGAACAAGTGAATCGTCAAATGATTAAATGGACGAAGCTTTATATTGATCTAGTTCTATCGAGTTGGATAATGCTGAAGCGGGAGCAAAGTTTTTACCATACATGGTTGCATTTAGCGCAACATGACCATAGtt                                                                                                         <  2:84934/141‑1 (MQ=255)

                             cACTTAGCGAACAAGTGAATCGTCAAATGATTAAATGGACGAAACTTTATATCGATCAATTTCTATCGAGTTGGACAATGCCGAAGCGTGAGCAAAGTTTTTACCATACATGGTTGCATTTAGCGCAACATGACCATAGtt                                                                                                         <  1:172196/141‑1 (MQ=255)

                                  aGCGAACAAGTGAATCGTCAAATGATTAAATGGACGAAACTTTATATCGATCAATTTCTATCGAGTTGGACAATGCCGAAGCGTGAGCAAAGTTTTTACCATACATGGTTGCATTTAGCGCAACATGACCATAGTTTTACt                                                                                                    <  1:167124/141‑1 (MQ=255)

                                           gTGAATCGTCAAATGATTAAATGGACGAAACCTTATATCGATCAATTTCTAGCGAGTTGGACAAGGCCGAAGCGTGAGCAAAGGTTTTACCATACATGGTTGCATTTAGCGCAACATGACCATAGTTTTACTAAAGCACAg                                                                                           >  2:107732/1‑141 (MQ=255)

                                             gAATCGTCAAATGATTAAATGGACGAAACTTTATATCGATCAATTTCTATCGAGTTGGACAATGCCGAAGCGTGAGCAAAGTTTTTACCATACATGGTTGCATTTAGCGCAACATGACCATAGTTTTACTAAAGCACAgcg                                                                                         >  1:120195/1‑141 (MQ=255)

                                              aaTCGTCAAATGATTAAATGGACGAAACTTTATATCGATCAATTTCTATCGAGTTGGACAATGCCGAAGCGTGAGCAAAGTTTTTACCATACATGGTTGCATTTAGCGCAACATGACCATAGTTTTACTAAAGCACAgcgc                                                                                        >  1:161284/1‑141 (MQ=255)

                                                   tCAAATGATTAAATGGACGAAACTTTATATGGATCAATTTCTATCGAGTTGGACAATGCCGAAGCGTGAGCAAAGTTTTTACCATACATGGTTGCATTTAGCGCAACATGACCATAGTTTTACTAAAGCACAGCGCCAAGt                                                                                   <  2:258015/141‑1 (MQ=255)

                                                      aaTGATTAAATGGACGAAACTATATATCGATCAATTTCTATCGAGTTGGACAATGCCGAAGCGTGAGCAAAGTTTTTACCATACATGGTTGCATTTAGCGCAACATGACCATAGTTTTACTAAAGCACAGCGCCAAGTGAt                                                                                <  2:165398/141‑1 (MQ=255)

                                                                 ggcagAACCTTTATATCGATCAATGTATATAGAGTTCGACGCTGAAGCAGCGTCCCCAAAAAAAGTACCGTACACGGTTGCCTCTTGTCCCATAGGAGCATAATTTTACTAAAGCACAGCGCCAAGTGATTAAAGGCTTAc                                                                     <  2:272783/137‑1 (MQ=255)

                                                                     gAAACTTTATATCGATCAATTTCTATCGAGTTGGACAATGCCGAAGCGTGAGCAAAGTTTTTACCATACATGGTTGCATTTAGCGCAACATGACCATAGTTTTACTAAAGCACAGCGCCAAGTGATTAAAGGCTTACCCaa                                                                 <  1:225410/141‑1 (MQ=255)

                                                                      aaaCTTTATATCGATCAATTTCTATCGAGTTGGACAATGCCGAAGCGTGAGCAAAGTTTTTACCATACATGGTTGCATTTAGCGCAACATGACCATAGTTTTACTAAAGCACAGCGCCAAGTGATTAAAGGCTTACCCAAt                                                                <  2:26565/141‑1 (MQ=255)

                                                                           ttATATCGATCAATTTCTATCGAGTTGGACAATGCCGAAGCGTGAGCAAAGTTTTTACCATACATGGTTGCATTTAGCGCAACATGACCATAGTTTTACTAAAGCACAGCGCCAAGTGATTAAAGGCTTACCCAATGATcc                                                           <  1:271051/141‑1 (MQ=255)

                                                                              tatCGATCAATTTCTATCGAGTTGGACAATGCCGAAGCGTGAGCAAAGTTTTTACCATACATGGTTGCATTTAGCGCAACATGACCATAGTTTTACTAAAGCACAGCGCCAAGTGATTAAAGGCTTACCCAATGATCCTGa                                                        >  1:278559/1‑141 (MQ=255)

                                                                              tatCGATCAATTTCTAACGAGATGGACAATGCCGAAGCGGGAGCAAAGTTTTTACCATACATGGTTGCATTTAGCGCAACATGACCATAGTTTTACTAAAGCACAGCGCCAAGTGATTAAAGGCTTACCCAATGATCCGGa                                                        >  2:145327/1‑141 (MQ=255)

                                                                                     cAATTTCTATCGAGTTGGAAAATGACGAAGCGTGAGCAAAGTTTTTACCATACATGGTTGCATTTAGCGCAACATGACCATAGTTTTACTAAAGCACAGCGCCAAGTGATTAAAGGCTTACCCAATGATCCTGAAATGACg                                                 <  1:9086/141‑1 (MQ=255)

                                                                                      aaTTTCTATTTGGTTGGACAATGCCGAAGCGTGAGCAAAGCTTTTACCATACATGGTTGCATTTAGCGCAACATGACCATAGTTCTACTAAAGCACAGCGCCAAGTGATTAAAGGCTTGCCCAATGATCCTGAAATGActa                                                <  2:220526/141‑3 (MQ=255)

                                                                                       aTTTCTATCGAGTTGGCCAATGCCGAAGCGTGAGCAAAGTTTTTACCATACATGGTTGCAATTAGCGCAACATGACCATAGTTTTACTAAAGCACGACGCCAAGTGATTAAAGGCTTACCCAATGATCCGGAAATGACGaa                                               >  2:267925/1‑140 (MQ=255)

                                                                                        tttCTATCGAGTTGGACAATGCCGAAGCGTGAGCAAAGTTTTTACCATACATGGTTGCATTTAGCGCAACATGACCATAGTTTTACTAAAGCACAGCGCCAAGTGATTAAAGGCTTACCCAATGATCCTGAAATGACGATa                                              <  1:203813/141‑1 (MQ=255)

                                                                                              tCGAGTTGGACAATGCCGAAGCGTGAGCAAAGTTTTTACCATACATGGTTGCATTTAGCGCAACATGACCATAGTTTTACTAAAGCACAGCGCCAAGTGATTAAAGGCTTACCCAATGATCCTGAAATGACGATAGAGTCa                                        >  1:3107/1‑141 (MQ=255)

                                                                                                      gACAATGCCGAAGCGTGAGCAAAGTTTTTACCATACATGGTTGCATTTAGCGCAACATGATCATAGTTTTACTAAAGCACAGCGCCAAGTGATTAAAGGCTTACCCAATGATCCTGAGATGACGATAGAGTCAGTATTAAc                                <  2:203383/141‑1 (MQ=255)

                                                                                                      gACAATGCCGAAGCGTGAGCAAAGTTTTTACCATACATGGTTGCATTTAGCGCAACATGACCATAGTTTTACTAAAGCACAGCGCCAAGTGATTAAAGGCTTACCCAATGATCCTGAAATGACGATAGAGTCAGTATTAAc                                <  1:202203/141‑1 (MQ=255)

                                                                                                       aCAATGCCGAAGCGTGAGCAAAGTTTTTACCATACATGGTTGCATTTAGCGCAACATGACCATAGTTTTACTAAAGCACAGCGCCAAGTGATTAAAGGCTTACCCAATGATCCTGAAATGACGATAGAGTCAGTATTAACt                               <  1:95382/141‑1 (MQ=255)

                                                                                                              cGAAGCGTGAGCAAAGTTTTTACCATACATGGTTGCATTTAGCGCAACATGACCATAGTTTTACGAAAGCACAGCGCCAAGTGATTAAAGGCTTACCCAATGATCCTGAAATGACGATAGAGTCAGTATTAACTCAttttt                        <  1:136886/141‑1 (MQ=255)

                                                                                                               gAAGCGTGAGCAAAGTTTTTACCATACATGGTTGCATTTAGCGCAACATGACCATAGTTTTACTAAAGCACAGCGCCAAGTGATTAAAGGCTTACCCAATGATCCTGAAATGACGATAGAGTCAGTATTAACTCATTTTTc                       <  1:46759/141‑1 (MQ=255)

                                                                                                                gaGCGTGAGCAAAGTTTTTACTACACATGGTTGCATTTATCGTAACATGACCATAGTTTTACTAAAGCACAGCGCCAAGTGATTAAAGGCTTACCCAATGATCCTGAAATGACGATAGAGTCAGTATTAACTCATTTTTCa                      <  2:14734/140‑1 (MQ=255)

                                                                                                                   ggTGAGCAAAGTTTTTACCATACATGGTTGCATTTAGCGCAACATGACCAGAGGTTTACTAAAGCACAGCGCCAAGTGATTAAAGGCTGACCCAATGATCCTTAAATGACGATAGAGTCAGTATTTACTCATTTTTCAATa                   <  2:197864/140‑1 (MQ=255)

                                                                                                                                    ccATACATGGTTGCATTTAGCGCACCATGACCATAGTTTTACTAAAGCACAGCGCCAAGTGATTAAAGGCTTACCCAATGATCCTGAAATGACGATAGAGTCAGTATTAACTCATTTTTCAATAGATCAGGAAGACTACCa  >  1:181184/1‑141 (MQ=255)

TGATGCAATAATAGATGAACAAGGTGAGCCACTTAGCGAACAAGTGAATCGTCAAATGATTAAATGGACGAAACTTTATATCGATCAATTTCTATCGAGTTGGACAATGCCGAAGCGTGAGCAAAGTTTTTACCATGCATGGTTGCATTTAGCGCAACATGACCATAGTTTTACTAAAGCACAGCGCCAAGTGATTAAAGGCTTACCCAATGATCCTGAAATGACGATAGAGTCAGTATTAACTCATTTTTCAATAGATCAGGAAGACTACCA  >  CP000730/479417‑479689

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`