BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP000730	36,015	C→T	29.0%	R179R (AGG→AGA)	USA300HOU_0026 ←	IS431mec transposase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	36,015	0	C	T	29.0%	453.5 / 228.8	334	R179R (AGG→AGA)	USA300HOU_0026	IS431mec transposase
Reads supporting (aligned to +/- strand): ref base C (121/114); new base T (40/57); total (162/172)
Fisher's exact test for biased strand distribution p-value = 9.27e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.67e-01

Only 100 of 338 total aligned reads displayed.

TTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTG‑ATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAGCCATTGCTACCTTCGTTGAAGGTGCCTGATC  >  CP000730/35877‑36155

 tAACTTGCTAGCATGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTG‑ATACTTTGATATCtt                                                                                                                                           >  1:240228/1‑141 (MQ=255)

  aaCTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTG‑ATACTTTGATACCTTg                                                                                                                                          >  2:123543/1‑141 (MQ=32)

   aCTTGCAAGCATGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTG‑ATACTTTGATATCTTGt                                                                                                                                         >  2:184078/1‑141 (MQ=11)

    cTTGCTAGCATGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATATATGTAACTCCGTGGAAATTATTCTTTGCCGTATTG‑ATACTTTGATATCTTGtc                                                                                                                                        >  1:25654/1‑141 (MQ=11)

     ttGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTG‑ATACTTTGATACCTTGtct                                                                                                                                       <  1:13887/141‑1 (MQ=17)

       gCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTG‑ATACTTTGATACCTTGtcttt                                                                                                                                     <  1:312004/141‑1 (MQ=32)

       gCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTG‑ATACTTTGATACCTTGtcttt                                                                                                                                     >  1:199532/1‑141 (MQ=17)

        cTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTG‑ATACTTTGATACCTTGtctttc                                                                                                                                    >  2:39557/1‑141 (MQ=17)

        cTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTG‑ATACTTTGATACCTTGtctttc                                                                                                                                    <  1:7345/141‑1 (MQ=17)

          aGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGGTGTATTG‑ATACTTTGATACCTTGtctttctt                                                                                                                                  >  1:45867/1‑141 (MQ=17)

           gCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTG‑ATACTTTGATACCTTGTCTTTCTTa                                                                                                                                 >  2:190284/1‑141 (MQ=17)

           gCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTG‑ATACTTTGATACCTTGTCTTTCTTa                                                                                                                                 <  1:136055/141‑1 (MQ=17)

              tgatgCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTG‑ATACTTTGATATCTTGTCTTTCTTACtt                                                                                                                              <  1:298833/141‑1 (MQ=11)

               gatgCTAATTTCGGGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTG‑ATACTTTGATATCTTGTCTTTCTTACttt                                                                                                                             <  2:120776/141‑1 (MQ=11)

                  gCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTG‑ATACTTTGATATCTTGTATTTCTTACTTTAat                                                                                                                          >  2:160235/1‑141 (MQ=11)

                    tAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTG‑ATACTTTGATACCTTGTCTTTCTTACTTTAatat                                                                                                                        >  2:52750/1‑141 (MQ=25)

                    tAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTG‑ATACTTTGATACCTTGTCTTTCTTACTTTAatat                                                                                                                        >  2:100829/1‑141 (MQ=21)

                       tttCGTGACATGGCGAAAATCCGGAGATCCCAAGAGACCTGCGGATATTTTTATATAGAGCGTAAATACACCCAATACCTTTTACAGTAGTCTGAACTGAATGG‑ATACCTTACTACCTCGTCTTTCTCACttaaccatgcc                                                                                                                     >  2:47485/1‑132 (MQ=2)

                        ttCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTCTATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTG‑ATACTTTGATATCTTGTCTTTCTTACTTTAATATGACg                                                                                                                    <  1:62918/141‑1 (MQ=11)

                         tCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTG‑ATACTTTGATACCTTGTCTTTCTTACTTTAATATGACgg                                                                                                                   <  1:193910/141‑1 (MQ=38)

                         tCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTG‑ATACTTTGATACCTTGTCTTTCTTACTTTAATATGACgg                                                                                                                   <  2:299381/141‑1 (MQ=37)

                          cGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTG‑ATACTTTGATCCCTTGTCTTTCTTACTTGAATATGACGGt                                                                                                                  <  2:138357/141‑1 (MQ=255)

                           gTGGCATTGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTG‑ATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTg                                                                                                                 <  2:260163/141‑1 (MQ=255)

                           gTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTG‑ATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTg                                                                                                                 >  2:310215/1‑141 (MQ=14)

                           gTGGCATGGCGAAAATCCGTAGATCTTAATAGACCTGCGGGTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTG‑ATACTTTGATTCCTTGTCTTTCTTACTTTAATATGACGGTg                                                                                                                 <  1:70347/141‑1 (MQ=11)

                           gTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTG‑ATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTg                                                                                                                 <  1:166577/141‑1 (MQ=39)

                            tGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCAGTCTTG‑TTACTTTGTTATCTTGTCTTTCTTACTTTAATATGACGGTgt                                                                                                                >  2:234114/1‑140 (MQ=11)

                             ggCATGGGGAAAATCCGTAGATCTGAAGAGCCCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTG‑ATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGttag                                                                                                               >  2:203015/1‑138 (MQ=11)

                               cATGGCGAAAATCCGTAGATCTGACGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTG‑ATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGATGATCt                                                                                                             >  2:44394/1‑141 (MQ=255)

                               cATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTG‑ATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCt                                                                                                             >  2:224842/1‑141 (MQ=255)

                               cATGGCGAAAATCCGGAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTACAGTATTCTTTGCTGTATTG‑ATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCt                                                                                                             <  1:32406/141‑1 (MQ=255)

                                aTGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTG‑ATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCtt                                                                                                            >  2:164842/1‑141 (MQ=255)

                                aTGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTG‑ATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCtt                                                                                                            >  2:281442/1‑141 (MQ=11)

                                aTGGGGAAAATCCGTAGATCTGAAGAGACCTACGGGTGTTTTGATATAGAGCATAAATACATTCAATACCTTTTAAAGTAGTCTTTGCCGTATTGCATA‑TTCGATGTCTTGTCTTTCTTACTTTAATATGTCGGGGCTCtt                                                                                                            >  2:103706/1‑141 (MQ=2)

                                aTGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTG‑ATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCtt                                                                                                            <  2:173174/141‑1 (MQ=255)

                                  ggggAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAACGTAAATACATTCAATCCCTTTTTAAGTATTCTTTGCCGTATTG‑AGACTTTGATATCTTGTCTTTATTACTTTAATATGACggggtacttac                                                                                                          >  2:9294/4‑132 (MQ=11)

                                    ttaaaactcttataTCTGAATATCCCTGCGGTTATTTTTATATAGAGCGTATATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTG‑ATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTc                                                                                                        <  2:261494/128‑1 (MQ=11)

                                     gAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTG‑ATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCa                                                                                                       <  1:183310/141‑1 (MQ=255)

                                       aaaTCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTG‑ATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATTTTGCTCAAt                                                                                                     >  2:126221/1‑141 (MQ=255)

                                         aTCCGTAGATCTGAATAGACCTGCCGTTCTTTTTATATAGTGCGTAAATACACTCAATACCTTTTACAGTATTCTTTGCTGTATTT‑ATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGa                                                                                                   <  2:169436/141‑1 (MQ=11)

                                         aTCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTG‑ATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGa                                                                                                   >  1:152037/1‑141 (MQ=255)

                                           ccGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTG‑ATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAgg                                                                                                 >  1:235827/1‑141 (MQ=39)

                                           ccGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTG‑ATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGccacatacgg                                                                                                 >  2:21540/1‑132 (MQ=255)

                                            cgcaGATCTGAAGAGACCTGCGGTTCTTTGTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTG‑ATACTTTGATACCTTGTCTTTCCTACTTTAATATGATGGTGCTCTTGCTCAATGAGGt                                                                                                <  1:171077/138‑1 (MQ=14)

                                            cGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTG‑ATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGt                                                                                                <  1:289801/141‑1 (MQ=255)

                                            cGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTG‑ATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGt                                                                                                >  2:205719/1‑141 (MQ=38)

                                            cGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTG‑ATAGTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGGTCTTGCTCAATGGGGt                                                                                                >  1:91761/1‑141 (MQ=11)

                                              tAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTG‑ATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTa                                                                                              <  2:144501/141‑1 (MQ=255)

                                                 aTCTGACGCGACCTGCGCTCCTTTTTATATAGACCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTG‑ATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTc                                                                                           <  1:189798/141‑1 (MQ=14)

                                                 aTCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTG‑ATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTc                                                                                           >  1:43951/1‑141 (MQ=38)

                                                     gAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTG‑ATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGat                                                                                       <  1:296632/141‑1 (MQ=255)

                                                     gAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTG‑ATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGat                                                                                       <  1:109372/141‑1 (MQ=255)

                                                         agaCCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTG‑ATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATAttt                                                                                   <  2:150899/141‑1 (MQ=255)

                                                              tGCGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTG‑ATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATg                                                                              >  2:63820/1‑141 (MQ=38)

                                                               tcGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTG‑ATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGt                                                                             <  2:240265/140‑1 (MQ=255)

                                                                   ttCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTG‑ATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACaa                                                                         <  1:279205/141‑1 (MQ=255)

                                                                    tCTTTTTATATAGATCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTG‑ATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAAt                                                                        <  1:282297/141‑1 (MQ=255)

                                                                    tCTTTTTATATAGAGCGTAAATACACTCAACACCTTTTAAAGTATTCTTTGCTGTATTG‑ATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTAAATTAGGTTATTCAGATATTTCGATGTACAAt                                                                        <  2:56784/141‑1 (MQ=255)

                                                                       ttttATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTG‑ATACTTTGATATCTTGTCTTTCTTACTTTACTATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGAc                                                                     >  2:246232/1‑141 (MQ=255)

                                                                        tttATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTG‑ATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACa                                                                    <  2:272027/141‑1 (MQ=255)

                                                                          tatataGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTG‑ATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGt                                                                  <  1:310215/141‑1 (MQ=255)

                                                                          tatataGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTG‑ATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGt                                                                  >  1:334687/1‑141 (MQ=255)

                                                                           atataGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTG‑ATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTc                                                                 >  1:115147/1‑141 (MQ=255)

                                                                              taGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTG‑ATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAgg                                                              >  2:317630/1‑141 (MQ=255)

                                                                                 agCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTG‑ATCCTTTGATTCCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGgttt                                                           <  1:246192/141‑1 (MQ=255)

                                                                                 agCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTG‑ATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGgttt                                                           <  2:20745/141‑1 (MQ=255)

                                                                                    ttgaaTGCACTCAATACCTTTTAAAGTATTCTTTGCTGTATTG‑ATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGgtttaag                                                        <  1:325437/138‑1 (MQ=255)

                                                                                      aaaTACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTG‑ATACTTTGCTATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACATTCAGgtttaagtt                                                      >  1:1113/1‑141 (MQ=255)

                                                                                       aaTACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTG‑ATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGgtttaagttt                                                     >  1:204924/1‑141 (MQ=255)

                                                                                       aaTACACTTAATACCTTTTAAAGTATTCTTTGCTGTATTG‑ATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGgtttaagttt                                                     <  2:129071/141‑1 (MQ=255)

                                                                                        aTACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTG‑ATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGgtttaagttta                                                    >  2:257378/1‑141 (MQ=255)

                                                                                         tACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTG‑ATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTCATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGgtttaagtttaa                                                   <  2:222816/141‑1 (MQ=255)

                                                                                         tACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTG‑ATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGgtttaagtttaa                                                   >  1:102723/1‑141 (MQ=255)

                                                                                          acacTCAATACCTTTTAAAGTATTCTTTGCTGTATTG‑ATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTaaa                                                  <  1:149122/141‑1 (MQ=255)

                                                                                           cactCAATACCTTTTAAAGTATTCTTTGCTGTATTG‑ATACTTTGATACCTTGTCTTTCTTACTTTCATATGCCGTTGATCTTACTCGATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTCTAAGTTTtaaa                                                 >  2:285670/4‑141 (MQ=14)

                                                                                             ctCAATACCTTTTAAAGTATTCTTTGCTGTATTG‑ATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGc                                               <  1:89109/140‑1 (MQ=255)

                                                                                              tCAATACCTTTTAAAGTACACTACAATTTATTT‑ATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCt                                              >  2:153061/1‑141 (MQ=14)

                                                                                               cAATACCTTTTAAAGTATTCTTTGCCGTATTG‑ATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCtt                                             <  1:274974/141‑1 (MQ=255)

                                                                                                   aCCTTTTAATGTATTCTTTGCCGTATTG‑ATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAAt                                         <  2:278624/141‑1 (MQ=255)

                                                                                                    ccTTTTAAAGTATTCTTTGCTGTATTG‑ATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCAATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGGTTTAAtt                                        >  1:255820/1‑141 (MQ=255)

                                                                                                    ccTTTTAAAGTATTCTTTGCCGTATTG‑ATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAAtt                                        >  1:123639/1‑141 (MQ=255)

                                                                                                    ccTTTTAAAGTATTCGTTGCTGTATTG‑ATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAAtt                                        <  1:232769/141‑1 (MQ=255)

                                                                                                        ttAAAGTATTCTTTGCTGTATTG‑ATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACtt                                    >  1:214394/1‑141 (MQ=255)

                                                                                                            aGTATTCTTTGCTGTATTG‑ATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTaac                                <  1:56837/141‑3 (MQ=255)

                                                                                                             gTATTCTTTGCCGTATTG‑ATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTCCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGcc                               <  2:253270/141‑1 (MQ=255)

                                                                                                               aTTCTTTGCTGTATTG‑ATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTCCTTTAACCAt                             >  1:302762/1‑141 (MQ=255)

                                                                                                               aTTCTTTGCCGTATTG‑ATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCAt                             >  2:3555/1‑141 (MQ=255)

                                                                                                                 tCTTTGCTGTATTG‑ATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTg                           >  2:269693/1‑141 (MQ=255)

                                                                                                                     tGCTGTATTG‑ATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTAc                       >  1:157517/1‑141 (MQ=255)

                                                                                                                             tG‑ATACTTTGATATCTTGTCTCTCTTACTTTAATATGACGGTGATTTTGCTCAATAAGGTTATTCAGATATTTCGATGTACACTAACAGTCAGGTCCAAGTTTAAAAGCTTTAATTATTTTAGCACTTGCTGCCTTCCTTg               >  2:7615/1‑141 (MQ=11)

                                                                                                                              g‑ATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCATTAATTACTTTAACCATTGCTACCTTCGTTGa              >  1:223444/1‑141 (MQ=255)

                                                                                                                                   cTTTGATATCTTGTCTTTCTTACTTTAATATGATGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGTAGGt          <  1:226655/141‑1 (MQ=14)

                                                                                                                                    tttGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTg         <  1:263986/141‑1 (MQ=255)

                                                                                                                                     ttGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTTCCTTCGTTGAAGGTGc        <  1:164842/141‑1 (MQ=255)

                                                                                                                                     ttGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGc        >  1:51263/1‑141 (MQ=255)

                                                                                                                                     ttGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGc        >  1:121072/1‑141 (MQ=255)

                                                                                                                                     ttGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAGCCATTGCTACCTTCGTTGAAGGTGc        <  1:276640/141‑1 (MQ=39)

                                                                                                                                       gATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATAGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTAATTTAACCATTGCTACCTTCGTTGAAGGTGCCt      >  2:327508/1‑141 (MQ=255)

                                                                                                                                         tACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGa    >  1:218675/1‑141 (MQ=255)

                                                                                                                                           ccTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATc  <  2:133051/141‑1 (MQ=255)

TTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTG‑ATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAGCCATTGCTACCTTCGTTGAAGGTGCCTGATC  >  CP000730/35877‑36155

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`