Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 36,161 T→C 30.7% I131V (ATT→GTT)  USA300HOU_0026 ← IS431mec transposase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP00073036,1610TC30.7% 184.9 / 152.0 203I131V (ATT→GTT) USA300HOU_0026IS431mec transposase
Reads supporting (aligned to +/- strand):  ref base T (48/90);  new base C (32/30);  total (80/123)
Fisher's exact test for biased strand distribution p-value = 2.93e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.15e-01

Only 100 of 206 total aligned reads displayed.

TTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAGCCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTC  >  CP000730/36022‑36301
                                                                                                                                            |                                                                                                                                            
 ttcttACTTTAATATGGCGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCCTTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTa                                                                                                                                            <  1:47713/141‑1 (MQ=255)
 ttcttACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTa                                                                                                                                            <  1:264823/141‑1 (MQ=255)
  tcttACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTAc                                                                                                                                           >  1:102862/1‑141 (MQ=255)
  tcttACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTAc                                                                                                                                           >  1:32714/1‑141 (MQ=255)
    ttACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATCACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCt                                                                                                                                         >  2:10054/1‑141 (MQ=255)
    ttACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCt                                                                                                                                         >  2:1357/1‑141 (MQ=255)
      atttaaaTATTACGGTGATCTTGCTCAATGAGTTTATTCAGATAGTTCGATGTACAATGGCACTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCttt                                                                                                                                       <  1:23223/136‑1 (MQ=11)
      aCTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTAGATGTACAATGTCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTCATTACCttt                                                                                                                                       >  1:64139/1‑141 (MQ=255)
         ttAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAGCCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGa                                                                                                                                    <  1:55812/141‑1 (MQ=35)
           aaTATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAGCCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTATTTACTttttaatg                                                                                                                                  >  1:169261/1‑137 (MQ=255)
               tGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTa                                                                                                                              <  1:241504/141‑1 (MQ=25)
               tGACGGTGATCTTGCTCAATGAGGTTATACAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTa                                                                                                                              >  1:211029/1‑141 (MQ=11)
                gatggTGATCTTGCTAAAGGTGGTTTTTCTGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAATGTGCCTGATCCGTAACTACCTTTTGAGGCTTAc                                                                                                                             <  1:161808/138‑1 (MQ=11)
                gACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTAc                                                                                                                             >  1:131094/1‑141 (MQ=255)
                gACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCAATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTCAc                                                                                                                             <  2:255820/141‑1 (MQ=255)
                 gcGGTGGTCTTGCGCAATGGGGTTATTCAGATATTTCCATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTCGAGGTTTAcc                                                                                                                            <  2:170946/140‑1 (MQ=11)
                 gcGGTGATCTTGCTCAATGAGGTTATTTAGTTATTTCGATGTACAATGGCAGTCACGTGTAAGTTTAATAGCTTTAATTTTTTGCATCATTGCTACCTTCGTTGAAGGTGCTTGATCTGTAATTACCTTTTGAGGTTTAcc                                                                                                                            <  2:201432/140‑1 (MQ=11)
                 aCGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGCTCTGTAATTATCTTTTGAGGTTTAcc                                                                                                                            >  1:74964/1‑141 (MQ=255)
                 aCGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTAcc                                                                                                                            >  2:126194/1‑141 (MQ=255)
                   ggTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCaa                                                                                                                          >  1:166910/1‑141 (MQ=18)
                    gTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCaaa                                                                                                                         >  2:173201/1‑141 (MQ=17)
                        tCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGt                                                                                                                     >  2:196145/1‑141 (MQ=255)
                        tCTTGCTCAATGAGGTTACTCCTGTAGTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGt                                                                                                                     >  2:322887/1‑141 (MQ=11)
                         cTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGCTTTAAGTGTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGATGCTTACCAAATTGtt                                                                                                                    <  1:246232/141‑1 (MQ=11)
                            gCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGGTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTaa                                                                                                                 <  1:277501/141‑1 (MQ=12)
                            gCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTaa                                                                                                                 >  1:143608/1‑141 (MQ=255)
                             gtCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAAt                                                                                                                <  2:224549/140‑1 (MQ=17)
                             cTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAAt                                                                                                                >  2:283287/1‑141 (MQ=11)
                                aaTGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATgag                                                                                                             >  1:74029/1‑141 (MQ=255)
                                  tGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACTAACTTGTTTAATGAGAc                                                                                                           >  2:301521/1‑141 (MQ=255)
                                  tGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCCGATCTGTAATTACCGTTTGAGGTTTACCAAATTGTTTAATGAGAc                                                                                                           >  2:210693/1‑141 (MQ=255)
                                    aGGTTATTCACGATTTGCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTCCCTTCGTTTTAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTTTCAAATTGTTTAATGAGACGt                                                                                                         <  2:6774/141‑1 (MQ=11)
                                    aGGTTATTCAGATATTTCGATGTACAATGCCGGTGAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGt                                                                                                         >  1:297244/1‑141 (MQ=255)
                                      gTTATTCAGATATTTGGATGTACTATGGCAGTCAGTTTTAAGTTTAAAAGCTTTAATTACTTTATCCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGttt                                                                                                       <  2:254785/141‑1 (MQ=11)
                                      gTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGttt                                                                                                       <  2:274263/141‑1 (MQ=17)
                                       ttATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTg                                                                                                      >  2:63283/1‑141 (MQ=255)
                                        tATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAACAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGa                                                                                                     <  1:190284/141‑1 (MQ=12)
                                         aTTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGAt                                                                                                    >  2:315650/1‑141 (MQ=12)
                                            cAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAGCCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATaaa                                                                                                 <  1:125785/141‑1 (MQ=32)
                                               gATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACg                                                                                               <  1:2305/141‑1 (MQ=17)
                                                atatTTGGATTTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGc                                                                                              <  2:42952/141‑1 (MQ=255)
                                                  atTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCat                                                                                            <  1:169761/141‑1 (MQ=255)
                                                      cGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGc                                                                                        >  2:51008/1‑141 (MQ=255)
                                                      cGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAGCCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGc                                                                                        <  2:247544/141‑1 (MQ=32)
                                                            aCAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCATTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATg                                                                                  >  2:267872/1‑141 (MQ=12)
                                                             cAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATgt                                                                                 >  1:334207/1‑140 (MQ=17)
                                                             cAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGGTTACCAAATTGTTTATTGAGACGTTTGATAAACGCATATGCTGAATGa                                                                                 >  1:288173/1‑141 (MQ=255)
                                                             cAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGa                                                                                 >  1:187051/1‑141 (MQ=255)
                                                             aaaTGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGa                                                                                 <  1:53548/140‑1 (MQ=11)
                                                              aaTGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGAt                                                                                >  1:1887/1‑141 (MQ=14)
                                                              aaTGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGAt                                                                                <  2:7722/141‑1 (MQ=14)
                                                                 gACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTAt                                                                             <  1:153061/141‑1 (MQ=11)
                                                                        agctttaactttaaAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTg                                                                      <  1:62272/138‑1 (MQ=0)
                                                                                                taccttatacATTGCTACCTTCTTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTCATAAACGCATATGCTGAATGATTATCTCGTTGCTAACGCAACCAAATACCTAatgt                                              <  2:309409/132‑1 (MQ=1)
                                                                                                   tttAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAatgtatg                                           <  1:184078/141‑1 (MQ=2)
                                                                                                   tttAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAatgtatg                                           >  1:175105/1‑141 (MQ=2)
                                                                                                   tttAGCCATTGCTACTCTCGTTGAAGATGCCTGATCTGGAATTACCTTTCGAGGTTTCCCAAATTGTTTAAGGGGACGTTTGCTACACGCATATTCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAatgtatg                                           <  1:168161/141‑1 (MQ=11)
                                                                                                     taaccATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTc                                         <  2:205712/138‑1 (MQ=17)
                                                                                                         cATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCctc                                     <  2:324879/141‑1 (MQ=32)
                                                                                                          aTTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCctct                                    <  1:10054/141‑1 (MQ=32)
                                                                                                          aTTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCctct                                    <  2:25642/141‑1 (MQ=32)
                                                                                                            tGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTgt                                  >  1:20090/1‑140 (MQ=17)
                                                                                                             gCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCa                                 <  1:322887/141‑1 (MQ=32)
                                                                                                             gCTACCTTCGTTGAAGGTGCCTGATCTGGAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAACCGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCa                                 <  2:234454/141‑1 (MQ=17)
                                                                                                              caaCCCTCGTTCAAGGTGCCTTATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCAt                                <  1:282392/139‑1 (MQ=255)
                                                                                                              cTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGATGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTgtat                                <  1:142533/141‑4 (MQ=17)
                                                                                                              cTACCTGCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCAt                                <  1:254890/141‑1 (MQ=17)
                                                                                                               tACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATc                               <  2:297244/141‑1 (MQ=17)
                                                                                                               tACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATc                               <  1:70902/141‑1 (MQ=32)
                                                                                                                gccTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCa                              >  1:83189/2‑141 (MQ=12)
                                                                                                                gccTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCa                              <  1:321185/140‑1 (MQ=255)
                                                                                                                gccTTCGGTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCa                              <  1:196145/140‑1 (MQ=255)
                                                                                                                 ccTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTAGCAAAGTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCaa                             >  2:218995/1‑141 (MQ=11)
                                                                                                                 ccTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCaa                             >  2:84090/1‑141 (MQ=12)
                                                                                                                  cTTCGGTGAAGGTGCCTGATCTTTAATTACCTTTTGACGTTCACCCTATTGTCCATTGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAAt                            <  1:44394/141‑1 (MQ=11)
                                                                                                                   ttCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATg                           <  1:304484/141‑1 (MQ=17)
                                                                                                                     cGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGc                         <  1:60567/141‑1 (MQ=12)
                                                                                                                     cGTTGAAGGTACCTGAACTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGATTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTCATGTATGTCCCCCTGTAGCAATGGc                         >  2:39148/1‑141 (MQ=11)
                                                                                                                      gTTGAAGGTGCCTGATCTGTAACTACCTTTTGACGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCa                        <  1:253370/141‑1 (MQ=255)
                                                                                                                         taaGGTGCCTGAGACGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGa                     <  1:100829/140‑1 (MQ=12)
                                                                                                                          aaGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGat                    >  1:203965/1‑141 (MQ=12)
                                                                                                                           agttGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGACACGata                   <  2:263008/138‑1 (MQ=17)
                                                                                                                           aGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGgta                   >  2:167456/1‑141 (MQ=17)
                                                                                                                            ggTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGatat                  >  1:11741/1‑141 (MQ=32)
                                                                                                                               gCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATaaa               >  2:152244/1‑141 (MQ=12)
                                                                                                                                 ccgATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATa             <  2:199076/139‑1 (MQ=17)
                                                                                                                                 cTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGATTCCGCAACCTCATATCGAATGTATGTCCCTCTGTATCAATGGCACGATATAAATa             <  2:321413/141‑1 (MQ=11)
                                                                                                                                  tGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAg            >  1:173125/1‑141 (MQ=255)
                                                                                                                                   gATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGc           <  2:333206/141‑1 (MQ=12)
                                                                                                                                   gATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGc           <  1:317900/141‑1 (MQ=255)
                                                                                                                                    aTCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCt          <  2:154675/141‑1 (MQ=17)
                                                                                                                                    aTCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCACTGGCACGATATAAATAGcc          >  2:160589/1‑140 (MQ=17)
                                                                                                                                     tCTGTTATTCCGTTGTAAGGTTTTCGAAATTGTTTACTGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTc         <  1:285670/141‑1 (MQ=11)
                                                                                                                                     tCTATAACTACCTTTTGAGGCTTACCCAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTAACGCATCCAAATAGCTAATGTATGTCCCTCTGTATCACTGGCACGATATAAATAGCTc         <  1:203015/141‑1 (MQ=11)
                                                                                                                                       tGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCa       >  1:205628/1‑141 (MQ=32)
                                                                                                                                       tGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGGATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCGTCTGTATCAATGTCACGATATAAATAGCTcaa       >  1:222863/1‑139 (MQ=11)
                                                                                                                                         tAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCAtt     >  2:240746/1‑141 (MQ=32)
                                                                                                                                         tAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTACCTCTGCATCAATGGCACGATCTAAATGGATCCAtt     >  2:258564/1‑141 (MQ=255)
                                                                                                                                          caTTACCTTTTGAGGTGTAGCAAATTGTTTAATGTGACTTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCAttt    <  1:41422/140‑1 (MQ=11)
                                                                                                                                            ttACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAAGGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTc  >  1:108500/1‑141 (MQ=17)
                                                                                                                                            |                                                                                                                                            
TTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAGCCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTC  >  CP000730/36022‑36301

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: