BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP000730	36,269	C→T	56.8%	A95T (GCA→ACA)	USA300HOU_0026 ←	IS431mec transposase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	36,269	0	C	T	56.8%	23.0 / inf	285	A95T (GCA→ACA)	USA300HOU_0026	IS431mec transposase
Reads supporting (aligned to +/- strand): ref base C (62/61); new base T (75/86); total (137/148)
Fisher's exact test for biased strand distribution p-value = 5.50e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.72e-01

Only 100 of 286 total aligned reads displayed.

GCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGT  >  CP000730/36130‑36409

gCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCa                                                                                                                                             <  1:322887/141‑1 (MQ=32)

  tACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATc                                                                                                                                           <  2:297244/141‑1 (MQ=17)

   gccTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCa                                                                                                                                          <  1:321185/140‑1 (MQ=255)

    ccTTCGTTGAAGGTGGCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCaa                                                                                                                                         <  2:224828/141‑1 (MQ=17)

    ccTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTAGCAAAGTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCaa                                                                                                                                         >  2:218995/1‑141 (MQ=11)

      ttCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATg                                                                                                                                       <  1:315650/141‑1 (MQ=32)

       tCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATgg                                                                                                                                      <  2:261433/141‑1 (MQ=32)

        cGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGc                                                                                                                                     <  1:60567/141‑1 (MQ=12)

          ttGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCAc                                                                                                                                   >  1:253605/1‑141 (MQ=12)

            taaGGTGCCTGAGACGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGa                                                                                                                                 <  1:100829/140‑1 (MQ=12)

             aaGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGat                                                                                                                                >  1:203965/1‑141 (MQ=12)

               ggTGCCTGATCTGTAACTACCGTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATAGGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGCCCCTCTGTCTCAATGGCACGgtat                                                                                                                              >  2:272955/1‑141 (MQ=11)

                gttgcTGATATGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGAtata                                                                                                                             <  1:187902/137‑1 (MQ=17)

                gTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGAtata                                                                                                                             >  1:45367/1‑141 (MQ=32)

                gTGCCTGATCGGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGAtata                                                                                                                             <  2:43951/141‑1 (MQ=17)

                  gCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATaaa                                                                                                                           >  2:152244/1‑141 (MQ=12)

                     tGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAg                                                                                                                        >  1:173125/1‑141 (MQ=255)

                      gATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGc                                                                                                                       <  1:317907/141‑1 (MQ=12)

                      gATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGc                                                                                                                       <  2:333206/141‑1 (MQ=12)

                       agcTGTAATTACCTGTTGAGGTTTATCAAATTTTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCt                                                                                                                      <  1:94329/139‑1 (MQ=12)

                       aTCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACTCAAGCAAATATCTAATGTATGTCCCTCTGTATCTATGGCACGATATAGATAGCt                                                                                                                      >  1:286960/1‑141 (MQ=11)

                        tCTGTTATTCCGTTGTAAGGTTTTCGAAATTGTTTACTGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTc                                                                                                                     <  1:285670/141‑1 (MQ=11)

                          tGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCa                                                                                                                   >  1:205685/1‑141 (MQ=32)

                           tgaactACCTTTTGGCGCTTACCAAATTGTTTAATGACACATTCGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATGTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCAt                                                                                                                  <  1:235536/136‑1 (MQ=11)

                            tAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCAtt                                                                                                                 >  2:240746/1‑141 (MQ=32)

                             aaTTAACTTTTGAGGTTTACCAAATTGTTTAAGGATACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCAttt                                                                                                                <  1:126221/141‑1 (MQ=12)

                              aTTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCAtttt                                                                                                               <  1:14313/141‑1 (MQ=17)

                               ttACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAAGGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTc                                                                                                              >  1:108500/1‑141 (MQ=17)

                                  ccTTTTGAGACTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATTTAATGTATGTTCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCtt                                                                                                           >  1:39245/1‑141 (MQ=11)

                                   cTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCttt                                                                                                          <  1:51008/141‑1 (MQ=17)

                                    ttttGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCtttt                                                                                                         >  2:92037/1‑141 (MQ=32)

                                     tttGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCttttt                                                                                                        >  2:168897/1‑140 (MQ=12)

                                       tGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTGtt                                                                                                      >  2:198064/1‑141 (MQ=17)

                                         agatttACCAAATTGTTGAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTAtttt                                                                                                    <  2:23783/138‑1 (MQ=17)

                                          ggTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTg                                                                                                   >  2:13283/1‑141 (MQ=32)

                                           gcttACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGa                                                                                                  <  1:240310/139‑1 (MQ=17)

                                             ttACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGCTATAAATAGCTCCATTTTCCTTTTATTTTGAta                                                                                                >  1:309368/1‑140 (MQ=17)

                                             ttACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGAta                                                                                                >  1:199281/1‑140 (MQ=17)

                                              tACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGAtat                                                                                               >  2:292830/1‑139 (MQ=17)

                                              tACCAAATTGTTTAATGAGACGTTTAATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGAtat                                                                                               <  1:44195/141‑3 (MQ=12)

                                               aCCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGAtata                                                                                              <  2:280093/141‑4 (MQ=17)

                                                       ttttAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCtcat                                                                                      <  1:131606/140‑1 (MQ=17)

                                                         ttAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCAATTTCCTTTTATTTTGATATACGTCtcatca                                                                                    >  2:125407/1‑141 (MQ=12)

                                                          tAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCaa                                                                                   <  2:307228/141‑1 (MQ=12)

                                                          tAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCaa                                                                                   >  1:135091/1‑141 (MQ=32)

                                                           aaTGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATAGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAAt                                                                                  <  1:154460/141‑1 (MQ=17)

                                                           aaTGAGACGTTAGATAAACGCATTTGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAAt                                                                                  <  1:223258/141‑1 (MQ=12)

                                                               ggaCGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGc                                                                              <  2:311367/140‑1 (MQ=17)

                                                                gaCGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATTTTCGTTTCATCAGTAAGcc                                                                             >  1:2019/1‑141 (MQ=11)

                                                                gaCGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGcc                                                                             <  1:324858/141‑1 (MQ=12)

                                                                gaCGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAAGGTATGTCCCTCTGTATCAATGGCACGATATACATACCTCCATTTACCTTTTATTTTGATATACGTCCCCTCAATACGcc                                                                             >  2:284078/1‑141 (MQ=11)

                                                                 aCGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTTATAAATTCGccc                                                                            >  1:119253/1‑140 (MQ=12)

                                                                    tttGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCTTTTTATTTTGATATACGTCTTATCAATACGCCCttt                                                                         >  1:72567/1‑141 (MQ=11)

                                                                       taTAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGta                                                                      <  1:150361/140‑1 (MQ=12)

                                                                       gATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTTATCAATAAGCAATTTGta                                                                      >  1:64207/1‑141 (MQ=11)

                                                                        aTAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGtaa                                                                     <  1:152545/141‑1 (MQ=17)

                                                                                 tatGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCGTATTCCTTTTATTTTGATGTATGTCTCATCAATACGCCATTTGTAATAAGCtttt                                                            >  2:183029/1‑141 (MQ=255)

                                                                                 tatGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCtttt                                                            >  2:267885/1‑141 (MQ=34)

                                                                                   tGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCtttttt                                                          >  2:59968/1‑141 (MQ=35)

                                                                                     cTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCCATACGCCATTTGTAATAAGCTTTTTTAt                                                        >  1:20834/1‑141 (MQ=255)

                                                                                        aaTGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCt                                                     <  1:217538/141‑1 (MQ=40)

                                                                                        aaTGATTATCTCGTTGCTTACGCAACAAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCt                                                     >  2:152601/1‑141 (MQ=255)

                                                                                         aTGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCtt                                                    <  2:203965/141‑1 (MQ=255)

                                                                                           gATTATCTCGTTGCTTACGCAACTAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCtttt                                                  <  2:157517/141‑1 (MQ=255)

                                                                                            tttATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCttttt                                                 <  2:5101/140‑1 (MQ=39)

                                                                                            aTTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGTTTTTTTATGCttttt                                                 <  1:248000/141‑1 (MQ=255)

                                                                                            aTTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCttttt                                                 <  2:107183/141‑1 (MQ=40)

                                                                                             ttATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTttc                                                >  1:273369/1‑141 (MQ=40)

                                                                                              tATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTttct                                               <  1:19054/141‑1 (MQ=255)

                                                                                              tATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATCAGCTTTTTTATGCTTTttct                                               >  2:89094/1‑141 (MQ=38)

                                                                                              tATCTCGTTGCTTAAGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTACAATGACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTttct                                               >  1:174089/1‑141 (MQ=14)

                                                                                              tATCTCGGTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTttct                                               <  2:166910/141‑1 (MQ=255)

                                                                                               aTCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTttctt                                              >  1:86463/1‑141 (MQ=39)

                                                                                                 ctcGGTGCTTACGCAACCAAATAGCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTcc                                            <  2:70796/141‑1 (MQ=255)

                                                                                                  tcGTTTCTTACGCAACCAAATATCTAATGTGTGTCCCTTTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCa                                           <  2:211029/141‑1 (MQ=14)

                                                                                                      tGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTCTCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAAtt                                       >  1:214127/1‑141 (MQ=255)

                                                                                                          tACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCCTTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGAt                                   >  2:302099/1‑141 (MQ=255)

                                                                                                            cGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAACACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATat                                 <  1:255546/141‑2 (MQ=255)

                                                                                                            cGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACTTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATat                                 <  1:163233/141‑2 (MQ=255)

                                                                                                                aCCAGATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTTATATACGTCTCATCAATACGCTATTTGTAATAAGCTTTTTTATGCTTTTTTTTCCAAATTTTATATaaaa                             >  1:227840/1‑141 (MQ=11)

                                                                                                                    aaTATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTAGGCTTTTTCTTCCAAATTTGATACAAAATTgg                         >  1:58527/1‑141 (MQ=255)

                                                                                                                      tatCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTgggg                       <  2:76509/141‑1 (MQ=37)

                                                                                                                        tCTAATGTATGTCCCTCTGTATCAATGGCCCGATATAACTAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCa                     <  1:8081/141‑1 (MQ=11)

                                                                                                                          tAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCata                   <  1:303111/141‑1 (MQ=255)

                                                                                                                          tAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTCATTCGTGTGATATAAAATTGGGGCata                   <  2:322458/141‑1 (MQ=11)

                                                                                                                          tAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCata                   <  1:267872/141‑1 (MQ=25)

                                                                                                                           aaTGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCatat                  <  1:99062/141‑1 (MQ=255)

                                                                                                                               tatctCCCTCTGTATCAATGGCACGATATAAATAGCTCCCTTTTCCTTTTATTTTGATATACGGCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCtt              <  2:143608/141‑1 (MQ=11)

                                                                                                                                atgtCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAAACAGAAATAAAATTTGCGCATATTCTTg             >  2:335227/1‑141 (MQ=11)

                                                                                                                                  gtCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTTATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGTTATAAAATTGGGGCATATTCTTGaa           >  1:260193/1‑141 (MQ=11)

                                                                                                                                  gtCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGaa           <  1:280508/141‑1 (MQ=255)

                                                                                                                                  gtCCCTCTGCATCAATGGCACGATAGAAATAGCTCCATTTTCCCTTTATTTTGATTTACGTCTCTTTAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATCCGAAATTTGGGCATATTCTTGaa           >  1:166273/1‑141 (MQ=14)

                                                                                                                                   tCCCTCTGCATCAATGGCACGATATAAATAGCTCGATTTTCCTTTTATTTTGATGTACGTCACATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACCAAATTGGGGCATGTTCTTTAAc          >  2:250447/1‑141 (MQ=14)

                                                                                                                                   tCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCCAATTTGATATAACATTGGGGCATGTTCTTGAAc          >  2:172040/1‑141 (MQ=255)

                                                                                                                                   ctccTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCAATTTGTAATCAGCTTTTTTTTGCTTTTTCTTCACAATTTGTTATAAAATTCGTGAATATGCTTGAAc          >  2:64981/3‑141 (MQ=11)

                                                                                                                                    cccTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTGTTGAAcc         >  2:122355/1‑141 (MQ=255)

                                                                                                                                    cccTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAAcc         >  2:15514/1‑141 (MQ=255)

                                                                                                                                    cccTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTTTTTAAcc         >  2:114038/1‑141 (MQ=255)

                                                                                                                                     ccTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTTTTGAAccc        >  1:237456/1‑141 (MQ=255)

                                                                                                                                        ctGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATAGACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGTGATAAAATTGGGGCATATTCTTGAACCCAAc     >  2:245737/1‑141 (MQ=11)

GCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGT  >  CP000730/36130‑36409

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`