BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP000730	36,315	G→A	58.3%	Y79Y (TAC→TAT)	USA300HOU_0026 ←	IS431mec transposase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	36,315	0	G	A	58.3%	82.6 / 267.5	274	Y79Y (TAC→TAT)	USA300HOU_0026	IS431mec transposase
Reads supporting (aligned to +/- strand): ref base G (51/59); new base A (86/70); total (142/132)
Fisher's exact test for biased strand distribution p-value = 1.72e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 275 total aligned reads displayed.

TTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATAT  >  CP000730/36175‑36452

  aCCAAATTTTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTa                                                                                                                                         <  1:173201/141‑1 (MQ=17)

       aTTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGTCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGtct                                                                                                                                    <  1:240159/141‑1 (MQ=12)

       aTTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATATGtat                                                                                                                                    >  1:279926/1‑139 (MQ=255)

       aTTGTTTAAGGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGtct                                                                                                                                    <  1:134818/141‑1 (MQ=12)

          ttttAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCtcat                                                                                                                                 <  1:131606/140‑1 (MQ=17)

          ttttAATGAGACGTTTGATAAACGCATATGCTGAAGGATTATCTCGTTGCTTACGCAACCAAACATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCtcat                                                                                                                                 <  2:240228/140‑1 (MQ=255)

            ttAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCAATTTCCTTTTATTTTGATATACGTCtcatca                                                                                                                               >  2:125407/1‑141 (MQ=12)

             tAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCaa                                                                                                                              >  2:152434/1‑141 (MQ=17)

              aaTGAGGCGTTTGATAGACGTATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCACTGGCGCTATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAAt                                                                                                                             <  1:47485/141‑1 (MQ=11)

              aaTGAGACGTTTGATATACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATAAAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAAt                                                                                                                             <  1:260706/141‑1 (MQ=17)

              aaTGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAAt                                                                                                                             <  1:286131/141‑1 (MQ=32)

              aaTGAGACGTTAGATAAACGCATTTGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAAt                                                                                                                             <  1:223258/141‑1 (MQ=12)

                   gaCGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATTTTCGTTTCATCAGTAAGcc                                                                                                                        >  1:2019/1‑141 (MQ=11)

                   gaCGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGcc                                                                                                                        <  1:324858/141‑1 (MQ=12)

                    aCGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTTATAAATTCGccc                                                                                                                       >  1:119253/1‑140 (MQ=12)

                      gTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCAtt                                                                                                                     >  1:55123/1‑141 (MQ=17)

                        ttGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTg                                                                                                                   <  1:263660/141‑1 (MQ=12)

                           aTAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGtaa                                                                                                                <  1:152545/141‑1 (MQ=17)

                                    tatGCTGAATGATTGTCTCATTGCTCACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTTATGTACGTCTCATCAATGCGCCATTTTTAATAAGCtttt                                                                                                       >  1:183138/1‑141 (MQ=11)

                                      tGCTGAATGATTATCTCTTTGTTTACGCAATCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCtttttt                                                                                                     <  2:169261/141‑1 (MQ=255)

                                      tGCTGAATGATTATATCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCtttttt                                                                                                     >  1:217931/1‑141 (MQ=37)

                                        cTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCCATACGCCATTTGTAATAAGCTTTTTTAt                                                                                                   >  1:20834/1‑141 (MQ=255)

                                          gAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTCGTAATAAGATTTTTTATGc                                                                                                 >  1:235171/1‑141 (MQ=255)

                                            aTGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCtt                                                                                               <  2:203965/141‑1 (MQ=255)

                                              gATTATCTCGTTGCTTACGCAACTAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCtttt                                                                                             <  2:157517/141‑1 (MQ=255)

                                               aTTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGTTTTTTTATGCttttt                                                                                            <  1:248000/141‑1 (MQ=255)

                                                 tATCTCTTTTCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATTGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTttct                                                                                          <  2:51263/141‑1 (MQ=255)

                                                 tATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCCATGGCACGAAATAAATAGCTCCATTTTACTTTTAGTATGAGGCAGCGCGCAGCAACACGCCCTCTGTAATAAGCTTTTTTTTGCTTTttct                                                                                          >  2:70047/1‑141 (MQ=14)

                                                 tATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTttct                                                                                          <  1:59968/141‑1 (MQ=40)

                                                 tATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAAAAAGCTTTTTTATGCTTTttct                                                                                          >  1:96147/1‑141 (MQ=255)

                                                  ttctcGTTGCTTACGCAACCAAATACCTAATGTATGTTCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTttctt                                                                                         <  1:153024/140‑1 (MQ=255)

                                                   tctcGTTGCTTTCGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTttcttc                                                                                        <  2:64139/141‑1 (MQ=255)

                                                   tctcGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTttcttc                                                                                        <  1:307844/141‑1 (MQ=255)

                                                     tcGTTTCTTACGCAACCAAATATCTAATGTGTGTCCCTTTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCa                                                                                      <  2:211029/141‑1 (MQ=14)

                                                      cGTGGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAACTAGCTCCTTTTGCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCaa                                                                                     <  2:285519/141‑1 (MQ=255)

                                                       gTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCaaa                                                                                    <  2:272466/141‑1 (MQ=255)

                                                         tGCTTACGCAACCAAATATCTAATGTATCTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTTTGTTTATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTTTGCTTTTTCTTCCAAAtt                                                                                  >  2:316326/1‑141 (MQ=11)

                                                           cTTACGCAACCAAATATCTAATGTATGTCCCCCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTg                                                                                <  2:74633/141‑1 (MQ=255)

                                                              aCGCAACCAAATACCTAATGTATGTCCCTCTGTATCAATAGCACGGTATAAATAGCTCCATTTTCCTTTTATTTTGTTATACGTCTCATCAATACGACATTTGTAATAAGCTTTTTTTAGCTTTTTTTTCCacaatctata                                                                             >  2:32288/1‑132 (MQ=11)

                                                               cGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAACACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATat                                                                            <  1:255546/141‑2 (MQ=255)

                                                               cGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACTTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATat                                                                            <  1:163233/141‑2 (MQ=255)

                                                                gCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACa                                                                           <  1:322392/141‑1 (MQ=255)

                                                                 cAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTATTTTATGCTTTTTCTTCCAAAAATGATACaa                                                                          >  1:282617/1‑141 (MQ=255)

                                                                       aaTATCTAATGTATGTCCCTCTGTATCAATCGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTgg                                                                    <  1:126194/141‑1 (MQ=255)

                                                                       aaTATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTgg                                                                    <  2:263515/141‑1 (MQ=38)

                                                                         tatCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTgggg                                                                  <  2:76509/141‑1 (MQ=37)

                                                                           tCTAATGTATGTCCCTCTGTATCAATGGCCCGATATAACTAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCa                                                                <  1:8081/141‑1 (MQ=11)

                                                                             tAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCata                                                              <  1:210693/141‑1 (MQ=38)

                                                                             tAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCata                                                              <  1:267872/141‑1 (MQ=25)

                                                                                 ggatgtCCCGCTGCATCAATGGGACGGTATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCt                                                          <  1:297911/139‑1 (MQ=255)

                                                                                  tatgtCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGTCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCtt                                                         <  2:91584/141‑1 (MQ=255)

                                                                                  tatctCCCTCTGTATCAATGGCACGATATAAATAGCTCCCTTTTCCTTTTATTTTGATATACGGCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCtt                                                         <  2:143608/141‑1 (MQ=11)

                                                                                   atgtCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAAACAGAAATAAAATTTGCGCATATTCTTg                                                        >  2:335227/1‑141 (MQ=11)

                                                                                    tgtCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTTTTTCAATTTTTATATAAAATTGGGGCATATttttta                                                       >  2:308611/1‑139 (MQ=11)

                                                                                     gtCCCTCTGCATCAATGGCACGATAGAAATAGCTCCATTTTCCCTTTATTTTGATTTACGTCTCTTTAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATCCGAAATTTGGGCATATTCTTGaa                                                      >  1:166273/1‑141 (MQ=14)

                                                                                      tCCCTCTGCATCAATGGCACGATATAAATAGCTCGATTTTCCTTTTATTTTGATGTACGTCACATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACCAAATTGGGGCATGTTCTTTAAc                                                     >  2:250447/1‑141 (MQ=14)

                                                                                      tCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCCAATTTGATATAACATTGGGGCATGTTCTTGAAc                                                     >  2:172040/1‑141 (MQ=255)

                                                                                       cccTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTGTTGAAcc                                                    >  2:122355/1‑141 (MQ=255)

                                                                                       cccTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTTTTTAAcc                                                    >  2:114038/1‑141 (MQ=255)

                                                                                        ccTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTTTTGAAccc                                                   >  1:237456/1‑141 (MQ=255)

                                                                                          tctGCATCAATGGCACGATATAAATCGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCaa                                                 >  1:57195/1‑141 (MQ=255)

                                                                                           ctgtaTCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTAAACCCAAc                                                >  1:64227/5‑141 (MQ=255)

                                                                                           ctGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATAGACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGTGATAAAATTGGGGCATATTCTTGAACCCAAc                                                >  2:245737/1‑141 (MQ=11)

                                                                                             gtaTCAATGGCACGATATAAATAGCTCCTTTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACgg                                              <  2:280509/139‑1 (MQ=255)

                                                                                             gtaTCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAAACCacagg                                              >  2:232545/3‑137 (MQ=11)

                                                                                             gtaTCAATGGCACGATATAAATAGCTCCATTTTCCTCTTATTTTGTTTTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCACCgg                                              >  2:131328/3‑141 (MQ=11)

                                                                                               aTCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTa                                            <  2:1902/141‑1 (MQ=21)

                                                                                               aTCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATGCGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTGTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTa                                            >  2:217892/1‑141 (MQ=255)

                                                                                               aTCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAATTTTGCTATAAAATTGGGGCATATTCTTAAACCCAACGGTa                                            >  1:103198/1‑141 (MQ=11)

                                                                                                 cAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACTGCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGa                                          >  1:315019/1‑141 (MQ=255)

                                                                                                 cAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGa                                          <  2:222863/141‑1 (MQ=18)

                                                                                                     ggCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAACTTGGGGCATATTCTTGAACCCAACGGTAGATCGt                                      >  1:17872/1‑141 (MQ=255)

                                                                                                        aCGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATATGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGttaa                                   >  2:196391/1‑139 (MQ=255)

                                                                                                            tataAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAatga                               <  1:189868/141‑1 (MQ=40)

                                                                                                            tataAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAatga                               >  1:27725/1‑141 (MQ=255)

                                                                                                            tataAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAatga                               >  2:29551/1‑141 (MQ=255)

                                                                                                              taAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAatgatg                             >  1:14262/1‑141 (MQ=255)

                                                                                                                   aGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGTGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGt                        >  2:47459/1‑141 (MQ=255)

                                                                                                                   aGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGt                        >  1:155524/1‑141 (MQ=255)

                                                                                                                    gCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATGAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGtt                       <  2:182092/141‑1 (MQ=14)

                                                                                                                      tCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTa                     >  2:281400/1‑141 (MQ=255)

                                                                                                                         aTTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTacac                  <  2:48820/141‑1 (MQ=255)

                                                                                                                           ttttCTTTTAGTTTGATATAGCTCTCATGAATACGCCTTTTGTAATCAGCTTTTTTATGCTTTTTCTTCCAAATTTGTTATCAAATTGGGGAATACTCTTGCACCCAACCGTAGATCGTTGAATGATGAACGTTTAcacca                <  2:292340/141‑1 (MQ=11)

                                                                                                                           tttCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTg                                                                     <  1:217230/88‑1 (MQ=255)

                                                                                                                           tttCCTTTCATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTAcacca                <  2:299846/141‑1 (MQ=255)

                                                                                                                            ttCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTAcacccc               >  1:81850/1‑139 (MQ=255)

                                                                                                                             tCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCCAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACg              >  2:85398/1‑141 (MQ=11)

                                                                                                                             tCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGATTTTTCTTCCAAATTTGATATAAAATTGGGTCATATTCTTGAACCCAACGGTAGATCGTTAAATGATGAACGGTTACACCACg              >  1:36757/1‑141 (MQ=11)

                                                                                                                              ccTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGt             >  1:113210/1‑141 (MQ=21)

                                                                                                                               cTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGtt            >  1:101741/1‑141 (MQ=255)

                                                                                                                                ttttATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGATCGTTTACACCACGTTc           <  1:198064/141‑1 (MQ=18)

                                                                                                                                  ttATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTccc         <  1:160589/141‑1 (MQ=18)

                                                                                                                                    aTTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCtt       <  1:152244/141‑1 (MQ=11)

                                                                                                                                      tttGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTaa     <  2:330369/141‑1 (MQ=40)

                                                                                                                                      tttGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTaa     <  1:296085/141‑1 (MQ=40)

                                                                                                                                       ttGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAat    >  2:186248/1‑141 (MQ=255)

                                                                                                                                        tGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGTTCGTTGAATGATGAACGTTTACACCACGTTCTCTTAata   <  1:9913/141‑1 (MQ=11)

                                                                                                                                         gATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACCCCACGTTCCCTTAatat  >  2:16329/1‑141 (MQ=25)

                                                                                                                                         gATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATGCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAatat  >  1:191789/1‑141 (MQ=11)

                                                                                                                                         gATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGAGATAAAATTGGGGCATATTCTTGAACCTAACGGTAGATCGTTGAATGATGAACGTTTTCACTTCGTTCTCTTAatat  >  1:271922/1‑141 (MQ=11)

TTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATAT  >  CP000730/36175‑36452

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`