BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP000730	36,378	C→T	82.5%	L58L (TTG→TTA)	USA300HOU_0026 ←	IS431mec transposase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	36,378	0	C	T	82.5%	332.8 / 91.1	233	L58L (TTG→TTA)	USA300HOU_0026	IS431mec transposase
Reads supporting (aligned to +/- strand): ref base C (17/23); new base T (97/91); total (115/118)
Fisher's exact test for biased strand distribution p-value = 3.84e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.64e-01

Only 100 of 233 total aligned reads displayed.

GCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATA  >  CP000730/36239‑36513

 cAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTATTTTATGCTTTTTCTTCCAAAAATGATACaa                                                                                                                                       >  1:282617/1‑141 (MQ=255)

   aCCAGATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTTATATACGTCTCATCAATACGCTATTTGTAATAAGCTTTTTTATGCTTTTTTTTCCAAATTTTATATaaaa                                                                                                                                     >  1:227840/1‑141 (MQ=11)

      aaaTGTCTAAGGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTg                                                                                                                                  <  2:131094/141‑1 (MQ=11)

       aaTATCTACTGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTgg                                                                                                                                 <  2:298913/141‑1 (MQ=255)

         tatCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTgggg                                                                                                                               <  2:76509/141‑1 (MQ=37)

           tCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCa                                                                                                                             <  2:175105/141‑1 (MQ=255)

            ttAATGGATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCCTTTTCCTCTTACTTTAATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCat                                                                                                                            <  1:174068/140‑1 (MQ=11)

            cTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCat                                                                                                                            <  1:152434/141‑1 (MQ=255)

             tAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTCATTCGTGTGATATAAAATTGGGGCata                                                                                                                           <  2:322458/141‑1 (MQ=11)

             tAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCata                                                                                                                           <  1:267872/141‑1 (MQ=25)

               atgtatgtCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTAGGGCTTAtt                                                                                                                         >  1:136013/1‑141 (MQ=11)

                 gtatgtCCCTCTGTATCAATGGCACGATATAAATAGATCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTCTGCTTTTTCTTCCAAATATTATATAATATTgggcgctattat                                                                                                                       >  2:149885/1‑132 (MQ=11)

                  tatgtCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGTCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCtt                                                                                                                      <  2:91584/141‑1 (MQ=255)

                   atgtCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCACATTTGATATAAAATTGGGGCATATTcgtt                                                                                                                     >  1:279697/1‑138 (MQ=11)

                    tgtCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTTTTTCAATTTTTATATAAAATTGGGGCATATttttta                                                                                                                    >  2:308611/1‑139 (MQ=11)

                     gtCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGaa                                                                                                                   >  1:148414/1‑141 (MQ=255)

                     gtCCCTCTGCATCAATGGCACGATAGAAATAGCTCCATTTTCCCTTTATTTTGATTTACGTCTCTTTAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATCCGAAATTTGGGCATATTCTTGaa                                                                                                                   >  1:166273/1‑141 (MQ=14)

                      tCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCACATTTGATATAAAATTGGGGCATATTCTTGAAc                                                                                                                  >  1:297838/1‑141 (MQ=255)

                      tCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAAc                                                                                                                  >  1:325258/1‑141 (MQ=255)

                      tCCCTCTGCATCAATGGCACGATATAAATAGCTCGATTTTCCTTTTATTTTGATGTACGTCACATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACCAAATTGGGGCATGTTCTTTAAc                                                                                                                  >  2:250447/1‑141 (MQ=14)

                      tCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCCAATTTGATATAACATTGGGGCATGTTCTTGAAc                                                                                                                  >  2:172040/1‑141 (MQ=255)

                      ctccTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCAATTTGTAATCAGCTTTTTTTTGCTTTTTCTTCACAATTTGTTATAAAATTCGTGAATATGCTTGAAc                                                                                                                  >  2:64981/3‑141 (MQ=11)

                       cccTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTGTTTTATGGTACGTGTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATTAAATTGGGGCATATTCTTGAAcc                                                                                                                 >  2:183352/1‑141 (MQ=11)

                       cccTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTTTTTAAcc                                                                                                                 >  2:114038/1‑141 (MQ=255)

                        ccTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTTTTGAAccc                                                                                                                >  1:237456/1‑141 (MQ=255)

                          tctGCATCAATGGCACGATATAAATCGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCaa                                                                                                              >  1:57195/1‑141 (MQ=255)

                          tctGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAACTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCAAATTCTTGAACCTaa                                                                                                              >  2:329750/1‑141 (MQ=255)

                           ctgtaTCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTAAACCCAAc                                                                                                             >  1:64227/5‑141 (MQ=255)

                               aTCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTa                                                                                                         <  2:1902/141‑1 (MQ=21)

                               aTCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATGCGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTGTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTa                                                                                                         >  2:217892/1‑141 (MQ=255)

                               aTCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAATTTTGCTATAAAATTGGGGCATATTCTTAAACCCAACGGTa                                                                                                         >  1:103198/1‑141 (MQ=11)

                                 cAATGGCACGATTAAAATAGCTTCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGa                                                                                                       <  2:187051/141‑1 (MQ=11)

                                 cAATGGCACGATATAAATAGCTTCATTTTCCTTTTATTTTGATCTACGTCTCATCAATACGCCATTTGTAATAAGCGTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGa                                                                                                       <  2:2019/141‑1 (MQ=255)

                                 cAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACTGCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGa                                                                                                       >  1:315019/1‑141 (MQ=255)

                                 cAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGa                                                                                                       <  2:222863/141‑1 (MQ=18)

                                     ggCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGt                                                                                                   <  1:109016/141‑1 (MQ=21)

                                     ggCACGATATAAATAGCTCCATTCTCCTTTTATTTTGATGTACGTCTCATCAATACGCTATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGt                                                                                                   <  1:210501/141‑1 (MQ=255)

                                             ataAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAatgat                                                                                           >  1:266052/1‑141 (MQ=21)

                                               aaaTAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAAtgatga                                                                                         >  1:284306/1‑141 (MQ=255)

                                                aaTAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATGTTCTTGAACCCAACGGTAGATCGTTGAATGATGaa                                                                                        >  1:188768/1‑141 (MQ=255)

                                                aaTAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGaa                                                                                        <  1:197174/141‑1 (MQ=255)

                                                   aGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGt                                                                                     >  1:155524/1‑141 (MQ=255)

                                                          ttttCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTTGGGCATGATCTTGAATCCAACTGTAGACCGTTGAATGATGAACGTTTAcacc                                                                              <  1:159860/141‑1 (MQ=11)

                                                           ttttCTTTTAGTTTGATATAGCTCTCATGAATACGCCTTTTGTAATCAGCTTTTTTATGCTTTTTCTTCCAAATTTGTTATCAAATTGGGGAATACTCTTGCACCCAACCGTAGATCGTTGAATGATGAACGTTTAcacca                                                                             <  2:292340/141‑1 (MQ=11)

                                                           tttCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTg                                                                                                                                  <  1:217230/88‑1 (MQ=255)

                                                            ttCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTAcacccc                                                                            >  1:81850/1‑139 (MQ=255)

                                                            ttCCGTTTATTTTGATATACGTCTCATCAATACGCCAGTTGTAATAAGCTTTTTTGTGCTTTTTCTTCCAAATTTGATATAGAATTGGGGCATATTCTTGAACCCAACGGTAGAACGTGGAATGATGAACGTTTAcaccac                                                                            <  2:200078/141‑1 (MQ=11)

                                                             tCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACg                                                                           <  1:302099/141‑1 (MQ=40)

                                                             tCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCCAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACg                                                                           >  2:85398/1‑141 (MQ=11)

                                                             tCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGATTTTTCTTCCAAATTTGATATAAAATTGGGTCATATTCTTGAACCCAACGGTAGATCGTTAAATGATGAACGGTTACACCACg                                                                           >  1:36757/1‑141 (MQ=11)

                                                             tCCTTTTATTTTCATATACGTCTCATCAATCCCCCTTTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGTGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACg                                                                           <  1:20913/141‑1 (MQ=11)

                                                              ccTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTAcaccacct                                                                          >  2:47182/1‑139 (MQ=255)

                                                              ccTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTACATCGTTGAATGATGAACGTTTACACCACGt                                                                          >  2:48351/1‑141 (MQ=255)

                                                               cTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGtt                                                                         >  1:101741/1‑141 (MQ=255)

                                                               cTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGtt                                                                         >  1:220924/1‑141 (MQ=255)

                                                                 tttATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTct                                                                       >  1:334111/1‑140 (MQ=255)

                                                                   tATTTTGATGTACGTCTCATTAATACGCGATTTGGTATAAACGTTTGAAAACTATTTCCACCAAGTTAGATTAAAAATTGGTGCATATGCTTGAACCTAACGTTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCt                                                                     <  2:34856/141‑1 (MQ=2)

                                                                        tgctGTACGTCTCATCAATACGCCATTTGTAATACGCTTTTTTATGGTTTTTGGTGGGGAGGCGGGGGAGAATTGGGGACTATTTTTGTTTCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAata                                                                <  2:235171/138‑1 (MQ=2)

                                                                        tGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTCGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAatt                                                                >  2:284567/1‑140 (MQ=11)

                                                                        tGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGTTCGTTGAATGATGAACGTTTACACCACGTTCTCTTAata                                                                <  1:9913/141‑1 (MQ=11)

                                                                         gATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACCCCACGTTCCCTTAatat                                                               >  2:16329/1‑141 (MQ=25)

                                                                         gATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAatat                                                               >  1:96160/1‑141 (MQ=40)

                                                                         gATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAatat                                                               >  1:4894/1‑141 (MQ=40)

                                                                         gATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATGCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAatat                                                               >  1:191789/1‑141 (MQ=11)

                                                                         gATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGAGATAAAATTGGGGCATATTCTTGAACCTAACGGTAGATCGTTGAATGATGAACGTTTTCACTTCGTTCTCTTAatat                                                               >  1:271922/1‑141 (MQ=11)

                                                                             tACGTCTCATTAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCa                                                           <  1:14499/141‑1 (MQ=255)

                                                                             tACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCa                                                           <  2:61622/141‑1 (MQ=255)

                                                                               cGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGa                                                         >  1:246980/1‑141 (MQ=40)

                                                                               cGTCTCATCAATACGCCATTTGTAATAAGCTCTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGa                                                         >  1:310478/1‑141 (MQ=255)

                                                                                gTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGat                                                        <  2:243565/141‑1 (MQ=21)

                                                                                   tcatcaATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGatata                                                     <  1:168897/141‑1 (MQ=255)

                                                                                   tcatcaATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGatata                                                     <  1:113884/141‑1 (MQ=18)

                                                                                   tcaataaTACGCCATTTGTAACAATCTTTTTTATGCTTTTTCTTCCAAATGTGCTATAAAATTGGGGCATATTCTTGAACCCAACGGCAGATAGTTGCATCATGAACGTTTACACCACGTTCTCTTAATATTGCAGatata                                                     <  2:11906/136‑1 (MQ=11)

                                                                                     atcgATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATc                                                   <  1:280928/141‑1 (MQ=255)

                                                                                     atcaATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATc                                                   <  2:212843/141‑1 (MQ=39)

                                                                                      tctaTACGCTTTTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCa                                                  <  1:124288/138‑1 (MQ=11)

                                                                                       caATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATCTATCAc                                                 >  1:307582/1‑141 (MQ=255)

                                                                                         aTACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGa                                               <  1:294474/141‑1 (MQ=255)

                                                                                         aTACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACAACGTTCTCTTAATATTCCAGATATATCACGa                                               >  2:254729/1‑141 (MQ=11)

                                                                                         aTACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTGGAATGATGAACGTGTACACCACGTTCTCTTAATATTTCAGATATATCACGa                                               >  2:28123/1‑141 (MQ=11)

                                                                                          tACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGAt                                              <  1:125407/141‑1 (MQ=255)

                                                                                          tACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGAt                                              <  2:25596/141‑1 (MQ=40)

                                                                                             gCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGAtcgc                                           >  2:134387/1‑138 (MQ=255)

                                                                                              ccATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATAGCt                                          >  2:179853/1‑141 (MQ=255)

                                                                                              ccATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACt                                          >  2:158763/1‑141 (MQ=25)

                                                                                                 tttGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATAGCTCaa                                       <  2:55123/141‑1 (MQ=11)

                                                                                                  ttGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATAGCTCAAt                                      <  2:14262/141‑1 (MQ=11)

                                                                                                  ttGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATAGCTCAAt                                      <  1:292311/141‑1 (MQ=11)

                                                                                                  ttGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATAGCTCAAt                                      <  2:309368/141‑1 (MQ=11)

                                                                                                  ttGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAAt                                      <  1:89094/141‑1 (MQ=21)

                                                                                                     taataaGCTTTTTTATGCTTTTTCTTCCAAGTTTGATATAAAATTGGGGCATATTCTTGACCCCAACGGTAGATCGTTGAATAATGAACGTTTACACCACGTTCTCTTAATATTACAGATATATCACGATAGCTAAATgca                                   >  1:72597/1‑139 (MQ=11)

                                                                                                     taataaGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGGAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGGTAACTCaaggca                                   >  2:276472/1‑137 (MQ=255)

                                                                                                     taataaGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGta                                   <  2:333585/141‑1 (MQ=40)

                                                                                                       ataaGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGtata                                 <  1:118692/141‑1 (MQ=40)

                                                                                                                tttATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGAta                        >  1:66598/1‑141 (MQ=35)

                                                                                                                  tATGCTTTTTCCTCCAAATTTGATACAAAATTGGGGCATATTCTTGACCCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGAtagt                      <  1:258564/141‑1 (MQ=25)

                                                                                                                  tATGCTTGTGGTTCTACATTTGCTACAAGAGGGGGGCATATTCTTGAACCCAACGGTTGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGAtagt                      <  1:240746/141‑1 (MQ=11)

                                                                                                                            cttcCATATTTGATACAAAATTGGGGCATATTCTTGAACCCACCGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTCATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGc            <  2:108500/141‑1 (MQ=255)

                                                                                                                             ttcCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAATGGCt           >  1:291155/1‑141 (MQ=25)

                                                                                                                                      tGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATa  >  1:323081/1‑141 (MQ=35)

GCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATA  >  CP000730/36239‑36513

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`