Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 478,502 C→A 100% D342E (GAC→GAA USA300HOU_0457 → NADH dehydrogenase (ubiquinone)

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP000730478,5020CA100.0% 83.2 / NA 29D342E (GAC→GAAUSA300HOU_0457NADH dehydrogenase (ubiquinone)
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (14/15);  total (14/15)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

TTCAATGTGCATTGGGTGTATATTCAGCAGCGATTATTCATTTAATATTGCACGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGACGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGTGACAGAAGTGCATATGAAGTGTTAAGTGCACTCATTCTAGGATGGTCATTACTTGTATCTTGGAATC  >  CP000730/478366‑478638
                                                                                                                                        |                                                                                                                                        
ttCAATGTGCATTGGGTGTATATTCAGCAGCGATTATTCATTTAATATTGCACGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCtt                                                                                                                                      >  1:295063/1‑141 (MQ=255)
  cAATGTGCATTGGGTGTATATTCAGCAGCGATTATTCATTTAATATTGCACGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTAt                                                                                                                                    >  1:38355/1‑141 (MQ=255)
       tgCATTGGGTGTATATTCAGCAGCGATTATTCATTTAATATTGCACGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCCTCTGCTAAAGAAGCTTATGGCTg                                                                                                                               >  1:292686/1‑141 (MQ=255)
         cATTGGGTGTATATTCAGCAGCGATTATTCATTTAATATTGCACGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGa                                                                                                                             >  1:123851/1‑141 (MQ=255)
               gtgtATATTCAGCAGCGATTATTCATTTAATATTGCACGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCa                                                                                                                       <  1:207359/141‑1 (MQ=255)
               gtgtATATTCAGCAGCGATTATTCATTTAATATTGCACGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCa                                                                                                                       <  1:29721/141‑1 (MQ=255)
                   tttttCAGCATCTGTTATTTATTTAATATTGCACGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATCTTCTAAGACAAGCATCTGCTAATGTAGCTTATGGCTGGATTGTCATggg                                                                                                                   <  2:152556/138‑1 (MQ=255)
                   atatTCAGCAGCGATTATTCATTTAATATTGCACGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATggg                                                                                                                   <  1:188157/141‑1 (MQ=255)
                      ttCAGCAGCGATTATTCATTTAATATTGCACGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCTTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACg                                                                                                                <  1:132879/141‑1 (MQ=255)
                            agcGATTATTCATTTAATATTGCACGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTAtt                                                                                                          >  2:101435/1‑141 (MQ=255)
                               gATTATTCATTTAATATTGCACGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCCTGGGACGTGTATTAGc                                                                                                       >  2:13148/1‑141 (MQ=255)
                                attattAATTTAATATTGCACGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCt                                                                                                      <  2:144168/141‑1 (MQ=255)
                                                    cGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTATATCGTGAAGCGATTTCATTTTTGAGAACAAGCAGCGGTGTAAGAATCTTATGGCGGGGTTGTCATAGGACGTGTATTAGCTATTATCCTGGCATTTCTAtt                                                                                  >  1:147636/1‑141 (MQ=255)
                                                     ggTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGGGAAGCGATTCAATATTCCCAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTAttt                                                                                 <  1:39598/141‑1 (MQ=255)
                                                     ggTATTTTAGAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTAttt                                                                                 <  1:309763/141‑1 (MQ=255)
                                                        aTTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTgg                                                                              <  2:268638/141‑1 (MQ=255)
                                                           tttAAAGCAACATTATTTTCACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCTTGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATg                                                                           <  2:219158/141‑1 (MQ=255)
                                                             tAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGGGTATTAGCTCTTATCGTGGCATTTCTATTTTGGATggg                                                                         >  2:243360/1‑139 (MQ=255)
                                                                       ttattTTTACAATCAGGGTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGTGACAGa                                                               <  2:123851/141‑1 (MQ=255)
                                                                                   tCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATTGATGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTCTTTTGGGTGAGTAGTGTCAGGAGGGCATATGag                                                   >  2:106398/1‑140 (MQ=255)
                                                                                      ggTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGGGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGTGACAGAAGTGCATATGAAgtg                                                >  1:124527/1‑141 (MQ=255)
                                                                                          cTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGTGACAGAAGTGCATATGAAGTGTTaa                                            >  1:86078/1‑141 (MQ=255)
                                                                                             tCGGGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGTGACAGAAGTGCATATGAAGTGTTAAGTg                                         <  1:155440/141‑1 (MQ=255)
                                                                                              tgttaaGTGATTCAATATGCCAAACAAAGCATCTGTTCAAGAAGTTTATGTCTTGATTGTCTTGGGACGTGTATTAGCTATGATCGTGGCATTTCTATTTTGGATGAGTAGTTACAGAAGTGCATATGAAGTTTTAAGTGc                                        <  2:303663/137‑1 (MQ=255)
                                                                                                 gAATCGATTTAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGTGACAGAAGTGCATATGAAGTGTTAAGTGCACt                                     <  2:128868/141‑1 (MQ=255)
                                                                                                                         aGCATCTTCTAAAGAAGCTTATGGCTTGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGTGACAGAAGTGCATATGAAGTGTTAAGTGCACTCATTCTAGGATGGTCATTACTTGt             <  2:295063/141‑1 (MQ=255)
                                                                                                                         aGCATCTGCTAAAGAAGCTTATGGCTGGCTTGTCCTGGGACGTGAATTAGGTATTCCGCTGGCATTTCTATTTTGGATGAGAAGTGACAGAAGTGCATCTGAAGTGTTAAGTGCACTACTTCTAGGATGTTAATGACTTGt             >  1:63385/1‑141 (MQ=255)
                                                                                                                          gCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGTGACAGAAGTGCATATGAAGTGTTAAGTGCACTCATTCTAGGATGGTCATTACTTGTa            >  2:73939/1‑141 (MQ=255)
                                                                                                                                   aaaGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGTGACAGAAGTGCATATGAAGTGTTAAGTGCACTCATTCTAGGATGGTCATTACTTGTATCTTGGAAt   >  2:31207/1‑141 (MQ=255)
                                                                                                                                    aagaagCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTAATTTGGATGAGTAGTGACAGAAGTGCATATGAAGTGTTAAGTGCACTCATGCTAGGATGGTCATACCTAGTAACTTGGaaac  >  2:227348/1‑139 (MQ=255)
                                                                                                                                        |                                                                                                                                        
TTCAATGTGCATTGGGTGTATATTCAGCAGCGATTATTCATTTAATATTGCACGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGACGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGTGACAGAAGTGCATATGAAGTGTTAAGTGCACTCATTCTAGGATGGTCATTACTTGTATCTTGGAATC  >  CP000730/478366‑478638

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: