BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP000730	35,970	T→C	52.4%	E194E (GAA→GAG)	USA300HOU_0026 ←	IS431mec transposase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	35,970	0	T	C	52.4%	25.5 / inf	268	E194E (GAA→GAG)	USA300HOU_0026	IS431mec transposase
Reads supporting (aligned to +/- strand): ref base T (60/67); new base C (74/66); total (134/134)
Fisher's exact test for biased strand distribution p-value = 3.92e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.73e-01

Only 100 of 270 total aligned reads displayed.

TCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAA  >  CP000730/35861‑36107

       aGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACt                                                                                                     >  2:2305/1‑141 (MQ=14)

        gtgtTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACtt                                                                                                    <  2:242828/141‑1 (MQ=255)

        gtgtTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACtt                                                                                                    <  2:165168/141‑1 (MQ=12)

        gtgtTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGCACAGACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACtt                                                                                                    <  1:175654/141‑1 (MQ=255)

         tgtTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACttt                                                                                                   >  1:115136/1‑141 (MQ=11)

         tgtTCGTCTAACTTGCTAGCATGATGCTAATTTCGGGGCATGGGTAAACTCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTGTTTGCCGTATTGATACttt                                                                                                   >  2:235536/1‑141 (MQ=11)

          gtTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTg                                                                                                  >  1:91551/1‑141 (MQ=17)

          gtTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACATTTTAAAGTATTCTTTGCTGTATTGATACtttt                                                                                                  >  1:277969/1‑140 (MQ=17)

            tCGTTTAACGTGCTAGCATGATGCTAATTGCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGAt                                                                                                <  1:254666/141‑1 (MQ=255)

             cgtttAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGTTa                                                                                               >  2:62272/4‑141 (MQ=12)

              ttttAACTTGCCAGCATGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATat                                                                                              <  2:310489/139‑2 (MQ=11)

              gtttAACTTGCTAGCATGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATat                                                                                              <  2:59971/139‑2 (MQ=11)

              gtttAACTTGCTAGCATGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATat                                                                                              <  1:279851/139‑2 (MQ=255)

              gtttAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTGACGTAGTCTTTGCTGTATTGGTACTTGGATAc                                                                                              >  1:21620/3‑141 (MQ=11)

                ttAACTTGCTAGCATGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATatct                                                                                            >  2:183310/1‑138 (MQ=255)

                  aaCTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTg                                                                                          >  2:123543/1‑141 (MQ=32)

                    cTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTggc                                                                                        >  1:204557/1‑139 (MQ=17)

                       gCTAGCATGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGtcttt                                                                                     <  2:209754/141‑1 (MQ=11)

                        cTAGCATGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGtctttc                                                                                    >  1:132981/1‑141 (MQ=255)

                        cTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGtctttc                                                                                    >  2:39557/1‑141 (MQ=17)

                         tcgCATGGTGCTAATTTCGTGGGATGGGGAAAATCCGTAGATCTTAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGtctttct                                                                                   <  2:148056/139‑1 (MQ=11)

                         tAGCATGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGtctttct                                                                                   <  1:294507/141‑1 (MQ=255)

                              tgatgCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACtt                                                                              >  2:238728/1‑141 (MQ=25)

                               ggtgCTAATGTCCTCGCATGGGGCACATCCGTAGATCTGAAGAGACCTACGGTTCCTTTTATATAGAGCGTAAATACATTCAATACCTTTTACAGTATTCTTTGCCGTATTGATACTTTGATATCTTTTCTTTCTTACttt                                                                             <  2:120777/139‑1 (MQ=11)

                               gatgCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACttt                                                                             <  2:265513/141‑1 (MQ=25)

                                 tgCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTaa                                                                           >  1:247614/1‑141 (MQ=21)

                                  gCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAat                                                                          >  1:299381/1‑141 (MQ=21)

                                    taagtgcGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAGTGTCTTTTAAAGTCTTCTTTGCTGTCTTTATACTTAGATACTTTGTCTTTCTTACTGTCatat                                                                        >  2:168161/7‑141 (MQ=11)

                                    tAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAatat                                                                        >  2:100829/1‑141 (MQ=21)

                                    tAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAatat                                                                        <  1:117968/141‑1 (MQ=25)

                                     aaTTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGCGACCTGCAGTTCTTTTTATATAGAGCGTTAATACCGCCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATg                                                                       <  1:159455/141‑1 (MQ=11)

                                       tttCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGAc                                                                     <  2:38184/141‑1 (MQ=25)

                                       tttCGTGACATGGCGAAAATCCGGAGATCCCAAGAGACCTGCGGATATTTTTATATAGAGCGTAAATACACCCAATACCTTTTACAGTAGTCTGAACTGAATGGATACCTTACTACCTCGTCTTTCTCACttaaccatgcc                                                                     >  2:47485/1‑132 (MQ=2)

                                        ttCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACg                                                                    >  1:107022/1‑141 (MQ=255)

                                        atCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTCTTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACg                                                                    >  2:14313/2‑141 (MQ=25)

                                         tCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACgg                                                                   >  1:36322/1‑141 (MQ=25)

                                         tCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACgg                                                                   <  1:193910/141‑1 (MQ=38)

                                          cGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGt                                                                  <  1:183660/141‑1 (MQ=38)

                                           gTGGCATGGCGAAAATCCGTAGATCTTAATAGACCTGCGGGTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATTCCTTGTCTTTCTTACTTTAATATGACGGTg                                                                 <  1:70347/141‑1 (MQ=11)

                                            tGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATCACGGTGa                                                                <  1:209436/141‑1 (MQ=255)

                                                 tGGGGAAAATCCGTAAATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTATTATGACGGTGATCTTg                                                           <  1:165294/141‑1 (MQ=14)

                                                 tGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATCCCTTGTCTTTCTTACTAAAATACGaacatgctgttg                                                           <  2:195056/141‑12 (MQ=11)

                                                  ggggAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAACGTAAATACATTCAATCCCTTTTTAAGTATTCTTTGCCGTATTGAGACTTTGATATCTTGTCTTTATTACTTTAATATGACggggtacttac                                                          >  2:9294/4‑132 (MQ=11)

                                                  ggCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGACCTTGc                                                          >  2:134818/1‑141 (MQ=255)

                                                   gCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTACAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCt                                                         >  1:63394/1‑141 (MQ=255)

                                                   gCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTAATCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCt                                                         >  1:224828/1‑141 (MQ=255)

                                                    ttaaaactcttataTCTGAATATCCCTGCGGTTATTTTTATATAGAGCGTATATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTc                                                        <  2:261494/128‑1 (MQ=11)

                                                       aaaTCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATTTTGCTCAAt                                                     >  2:126221/1‑141 (MQ=255)

                                                       aaaTCCGCAGATCTAAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAAt                                                     <  2:151065/141‑1 (MQ=11)

                                                        aaTCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATg                                                    >  2:222587/1‑141 (MQ=38)

                                                        aaTCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATg                                                    >  1:333206/1‑141 (MQ=255)

                                                        aaTCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATg                                                    <  2:223073/141‑1 (MQ=255)

                                                         aTCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGa                                                   >  1:152037/1‑141 (MQ=255)

                                                          tCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAg                                                  <  1:5660/141‑1 (MQ=38)

                                                            cgcaGATCTGAAGAGACCTGCGGTTCTTTGTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCCTACTTTAATATGATGGTGCTCTTGCTCAATGAGGt                                                <  1:171077/138‑1 (MQ=14)

                                                            cGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGt                                                >  2:205719/1‑141 (MQ=38)

                                                            cGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGt                                                >  2:257062/1‑141 (MQ=37)

                                                            cGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGt                                                >  1:111078/1‑141 (MQ=255)

                                                            cGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGt                                                >  2:296928/1‑141 (MQ=255)

                                                              tAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTGAAGTATGCTTTGCTGTATTGATACTTTGATCCCTTGTCTTTCTTACTTTAATATGAAGGTTACATTGCttcaggaagtta                                              >  2:282392/1‑130 (MQ=11)

                                                               aGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTAt                                             >  1:261433/1‑141 (MQ=38)

                                                                gATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTAtt                                            >  1:321413/1‑141 (MQ=255)

                                                                 aTCTGTAGAGATCTGCTGTTCTTTTTATATATGGGGTATATACACCCAATACCTTTTAAAGTATTCTTTGCTGTACTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTc                                           <  2:319474/141‑1 (MQ=11)

                                                                 aTCTGACGCGACCTGCGCTCCTTTTTATATAGACCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTc                                           <  1:189798/141‑1 (MQ=14)

                                                                 aTCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTc                                           <  2:248584/141‑1 (MQ=255)

                                                                  gcTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCa                                          >  2:161626/2‑141 (MQ=37)

                                                                   cTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTGTGCTGTATTGATACTTTGTTACCTTGTCTTTCTTACTTTAATATGACGGTGCTCTTGCTCCATGAGGTTATTCAg                                         >  2:101734/1‑141 (MQ=255)

                                                                   cTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATTCCTGGTCTTTCTTACTTTAATCTGCCGGTGATCTTGCTCAATGAGGTTATTCAg                                         >  1:44468/1‑141 (MQ=11)

                                                                    tGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGTTCAATGAGGTTATTCAGa                                        <  2:131659/141‑1 (MQ=255)

                                                                    tGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGa                                        <  1:139769/141‑1 (MQ=255)

                                                                      aaGAGACCTGCGGGTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATTTGACGGGTATCTTGCTCAATGAGGtactccagact                                      >  2:50374/1‑131 (MQ=11)

                                                                        aagaTCTGCGGTTCTTTTTGATTCTCGCGGAGATACGCTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATAtt                                    <  2:68144/140‑1 (MQ=14)

                                                                          gaCCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGCGGGTATTCAGACTTTTc                                  >  2:187902/1‑141 (MQ=11)

                                                                             cTGCGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGAt                               <  2:329679/141‑1 (MQ=38)

                                                                             cTGCGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGAt                               >  2:104684/1‑141 (MQ=38)

                                                                               gCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTTTTGTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGt                             <  1:75815/141‑1 (MQ=255)

                                                                               gCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGt                             >  1:234454/1‑141 (MQ=38)

                                                                                cGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTa                            <  1:123543/141‑1 (MQ=39)

                                                                                    tCTTTTTATATAGATCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAAt                        <  1:282297/141‑1 (MQ=255)

                                                                                    tCTTTTTATATAGAGCGTAAATACACTCAACACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTAAATTAGGTTATTCAGATATTTCGATGTACAAt                        <  2:56784/141‑1 (MQ=255)

                                                                                      tttttATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGGTCTTGCTCAATGAGGTTATTCAGATATTTCGTTGTACAATgg                      >  2:259744/1‑140 (MQ=255)

                                                                                       ttttATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGAc                     <  1:276466/141‑1 (MQ=255)

                                                                                        tttATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACa                    <  2:272027/141‑1 (MQ=255)

                                                                                         ttATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAg                   <  1:136797/141‑1 (MQ=255)

                                                                                             ttaGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAg               <  2:309578/140‑1 (MQ=255)

                                                                                               agagCGTAAATACACTCAATACATTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGTTGATCTTGCTCATTGCAGTTATTCAGATATTTCGATGTACAATGGCAGACAGgt             >  2:44202/1‑141 (MQ=14)

                                                                                                 agCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATCCTTTGATTCCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGgttt           <  1:246192/141‑1 (MQ=255)

                                                                                                  gCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGgtt            <  1:284054/139‑1 (MQ=255)

                                                                                                    ttgaaTGCACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGgtttaag        <  1:325437/138‑1 (MQ=255)

                                                                                                     tAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGgtttaagt       >  1:23783/1‑141 (MQ=255)

                                                                                                     tAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACATTGTATTTCTTACTTTAATATGATGGTGATCTTGCTCAATGATGTTATTCAGATATTTCGATGTACAATGGCCGTCAGgtttaggt       >  2:286131/1‑141 (MQ=14)

                                                                                                       aaTACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGgtttaagttt     <  1:304415/141‑1 (MQ=255)

                                                                                                        aTACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGgtttaagttta    >  1:169799/1‑141 (MQ=255)

                                                                                                        aTACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATGCAGATATTTCGATGTACAATGACAGTCAGgtttaagttta    >  2:169761/1‑141 (MQ=255)

                                                                                                         tACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATTACAGTCAGgtttaagtttaa   >  1:311367/1‑141 (MQ=255)

                                                                                                         tACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGgtttaagtttaa   >  1:102723/1‑141 (MQ=255)

                                                                                                         tACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCCGTCAGgtttaagtttaa   >  1:4759/1‑141 (MQ=255)

                                                                                                         tACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGgtttaagtttaa   >  1:219076/1‑141 (MQ=255)

                                                                                                          acacTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTaaa  <  2:278805/141‑1 (MQ=255)

                                                                                                          acacTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTaaa  <  1:149122/141‑1 (MQ=255)

TCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAA  >  CP000730/35861‑36107

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`