Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 2,335,371 A→G 21.2% intergenic (+376/+784) USA300HOU_2190 → / ← USA300HOU_2192 Sir2 family NAD‑dependent deacetylase/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007302,335,3710AG21.2% 24.7 / 5.3 19intergenic (+376/+784)USA300HOU_2190/USA300HOU_2192Sir2 family NAD‑dependent deacetylase/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (10/5);  new base G (2/2);  total (12/7)
Fisher's exact test for biased strand distribution p-value = 6.03e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.96e-01

GATTAAAAATAAACATAAAATAAGTAGAAAAATAAAAGCTACGAATATTAAAAAATTAGATATCTTCATTATCAAATTCCGATATTAAATCATTATATTTTTCAGAATATCTTGCATAATCTTCATTATTATTAAAAGCTAGCCATAGACCTCTATCATCATATAAATGATATATTAAGTTTTTAGTATTGTTAATTATATATATTTCAGAATAATTATCATTCTGATTGATATTTGGAACTAACCCTTTAAAGTCTTGATTGGATATAGCTTT  >  CP000730/2335231‑2335504
                                                                                                                                            |                                                                                                                                     
aaatAAAAATAAACATAAAATAAGTAGAAAAATAAAAGCTACGAATATTAAAAAATTAGATATCTTCATTATCAAATTCCGATATTAAATCATTATATTTTTCAGAATATCTTGCATAATCTTCATTATTATTAAAAGCTa                                                                                                                                       <  2:64170/138‑1 (MQ=255)
           aaCATAAAATAAGTAGAAAAATAAAAGCTACGAATATTAAAAAATTAGATATCTTCATTATCAAATTCCGATATTAAATCATTATATTTTTCAGAATATCTTGCATAATCTTCATTATTATTAAAAGCTAGCCATAGAcat                                                                                                                            >  1:88761/1‑139 (MQ=255)
                aaaaTAAGTAGAAAAATAAACGCTACGAATATTAAAAAATTAGATATCTTCATTATCAAATTCCGATATTAAATCATTATATTTTTCAGAATATCTTGCATAATCTTCATTATTGTTAAAAGCTGGCCATAGACCTCTatc                                                                                                                       >  1:201631/1‑141 (MQ=255)
                aaaaTAAGTAGAAAAATAAAAGCTACGAATATTAAAAAATTAGATATCTTCATTATCAAATTCCGATATTAAATCATTATATTTTTCAGAATATCTTGCATAATCTTCATTATTATTAAAAGCTGGCCATAGACCTCTatc                                                                                                                       >  1:62868/1‑141 (MQ=255)
                     aaGTAGAAAAATAAAAGCTACGAATATTAAAAAATTAGATATCTTCATTATCAAATACCGATATTAAATCATTATATTTTTCAGAATATCTTGCATAATCTTCATTATTATTAAAAGCTAGCCATAGACCCCTATcatcat                                                                                                                  >  2:77392/1‑141 (MQ=255)
                     aaGTAGAAAAAGAAAAGCTACGAATATTAAAAAATTAGATTTCTTCATTATCAAATTCCGATATTAAATCATTATATTTTTCAGAATATCTTGCATAATCTTCATTATTATTAAAAGCTAGCCATAGACCTCTATcatcat                                                                                                                  <  2:113938/141‑1 (MQ=255)
                       gTAGAAAAATAAAAGCTACGAATATTAAAAAATTAGATATCTTCATTATCAAATTCCGATATTAAATCATTATATTTTTCAGAATATCTTGCATAATCTTCATTATTATTAAAAGCTAGCCATAGACCTCTATCATCatat                                                                                                                <  1:316668/141‑1 (MQ=255)
                              aaTAAAAGCTACGAATATTAAAAAATTAGATATCTTCATTATCAAATTCCGATATTAAATCATTATATTTTTCAGAATATCTTGCATAATCTTCATTATTATTAAAAGCTAGCCATCGACCTCTATCATCATATAAATGat                                                                                                         >  2:51864/1‑141 (MQ=255)
                                          aaaTATTAAAAAATTAGATATCTTCATTATCAAATTCCGATATTAAATCATTATATTTTTCAGAATATCTTGCATAATCTTCATTATTATTAACATTTAGCCATAGACCTCTATCATCATATAAATGATATATTAAGtttt                                                                                             <  1:260470/140‑1 (MQ=255)
                                                 aaaaaaTGTGATATCTTGATTCTCAAATTCCGATATTAAACCATTATATTTTTCAGAATATCTTGCATGATCTTCATTATTATTAAAAGCTAGCCATCGACCTCTATCATCATATAAATGATATATTAAGTTTTTAGTAtt                                                                                      <  2:81795/141‑1 (MQ=255)
                                                                    ttATCAAATTCCGATATTAAATCATTATATTTTTCAGAATATCTTGCATAATCTTCATTATTATTAAAAGCTAGCCATAGACCTCTATCATCATATAAATGATATATTAAGTTTTTAGTATTGTTAATTATATATATTTCa                                                                   >  2:237504/1‑141 (MQ=255)
                                                                         aaaTTCCGATATTAAATCATTATATTTTTCTGTATTTTTTGCATAATCTTCATTATTATTAAAATCTAGCCATAGACCTCTATCATCATATAAATGATATATTAAGTTTTTAGTATTTTTAATTATATATATTTCaaattt                                                              >  2:126850/1‑136 (MQ=255)
                                                                                                            atCTTGCATAATCTTCATTATTATTAAAAGCTAGCCATAGACCTCTATCATCATATAAATGATCTATTAAGTTTTTAGTATTGTTAATTATATATATTTCAGAATAATTATCATTCTGATTGATATTTGGAACTAAACCtt                           >  2:53064/1‑141 (MQ=255)
                                                                                                                     aaTCTTCATTATTATTAAAAGCTGGCCATAGACCTCTATCATCATATAAATGATATATTAAGTTTTTAGTATTGTTAATTATATATATTTCAGAATAATTATCATTCTGATTGATATTTGGAACTAACCCTTTAAAGTCtt                  <  2:62868/141‑1 (MQ=255)
                                                                                                                     aaTCTTCATTATTATTAAAAGCTGGCCATAGACCTCTATCATCATATAAATGATATATTAAGTTTTTAGTATTGTTAATTATATATATTTCAGAATAATTATCATTCTGATTGATATTTGGAACTAACCCTTTAAAGTCtt                  <  2:201631/141‑1 (MQ=255)
                                                                                                                       tCTTCATTATTTTTAAAAGCTAAACGCAGACCTCTATCATCATATAAATGATATATTAAGTTTTTAGTATTGTTAATTATATATATTTCAGAATAATTATCATTCTGATTGATATTTGGAACTAACCCTTTAAAGTCTTGa                >  1:161232/1‑141 (MQ=255)
                                                                                                                        cTTCATTATTATTAAAAGCTAGCCATAGACCTCTATCATCATATAAATGATATATTAAGTTTTTAGTATTGTTAATTATATATATTTCAGAATAATTATCATTCTGATTGACATTTCCAAGTAACACTTTAAAGTGGTGAt               >  2:335258/1‑141 (MQ=255)
                                                                                                                               attattaAAAGCTAGCCATAGACCTCTATCATCATATAAATGATATATTAAGTTTTTAGTATTGTTAATTATATATATTTCAGAATAATTATCATTCTGATTGATATTTGGAACTAACCCTTTAAAGTCTTGATTGGatat        >  1:164902/1‑141 (MQ=255)
                                                                                                                                   ttaAAAGCTAGCCATAGACCTCTATCATCATATAAATGATATATTAAGTTTTTAGTATTGTTAATTATATATATTTCAGAATAATTATCATTCTGATTGATATTTGGAACTAACCCTTTAAAGTCTTGATTGGATATAGCt    >  1:324258/1‑141 (MQ=255)
                                                                                                                                     aaaaGCTAGCCATAGACCTCTATCATCATATAAATGATATATTAAGTTTTTAGTATTGTTAATTATATATATTTCAGAATAATTATCATTCTGATTGATATTTGGAACTAACCCTTTAAAGTCTTGATTGGATATAGCttt  <  1:63640/141‑1 (MQ=255)
                                                                                                                                            |                                                                                                                                     
GATTAAAAATAAACATAAAATAAGTAGAAAAATAAAAGCTACGAATATTAAAAAATTAGATATCTTCATTATCAAATTCCGATATTAAATCATTATATTTTTCAGAATATCTTGCATAATCTTCATTATTATTAAAAGCTAGCCATAGACCTCTATCATCATATAAATGATATATTAAGTTTTTAGTATTGTTAATTATATATATTTCAGAATAATTATCATTCTGATTGATATTTGGAACTAACCCTTTAAAGTCTTGATTGGATATAGCTTT  >  CP000730/2335231‑2335504

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: