| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP000730 | 2,679,847 | G→A | 100% | G111S (GGT→AGT) | USA300HOU_2536 → | possible secretory antigen |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP000730 | 2,679,847 | 0 | G | A | 91.7% | 64.0 / ‑0.8 | 24 | G111S (GGT→AGT) | USA300HOU_2536 | possible secretory antigen |
| Reads supporting (aligned to +/- strand): ref base G (1/1); new base A (7/15); total (8/16) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.86e-01 | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
ATGCTAAAAATTGGTATTACAATGCACGTAAATCAAAATATGCGACTGGTCGTACACCAAGAAAATTTGCTGTCATGCAATCAACTGCAGGATATTATGGACATGTCGCAGTTGTTGAACAAGTATATAAAAACGGTAGTATTAAAGTTTCAGAATACAACTTTTATCGCCCATTAAAATACAATACACGTGTACTAAGCAAAAAGGCAGCACGTAACTTTAACTATATTTACTAATCAAAAAACTTCTATCACGAACGCTTCAATTT > CP000730/2679713‑2679980 | aTGCTAAAAATTGGTATTACAATGCACGTAAATCAAAATATGCGACTGGTCGTACACCAAGAAAATTTGCTGTCATGCAATCAACTGCAGGATATTATGGACATGTCGCAGTTGTTGAACAAGTATATAAAAACAgtagta > 1:116622/1‑141 (MQ=255) aaaaaTTGGGATTACAATGCACGTAAATCAAAATATGCGACTGGTCGTACACCAAGAAAATTTGCTGTCATGCAATCAACTGCAGGATATTATGGACATGTCGCAGTTGTTGAACAAGTATATAAAAACAGTAGTATTaaa < 1:131622/141‑1 (MQ=255) ttGGTATTCAAATGCACGTAAATCAAAATAGGCGACTGGTCGTACACCAACAAAATTTGCTGTCATGCAATCAACTGCAGGATATTATGGACATGTCGCAGTTGTTGAACAAGTATATAAAAACAGTAGTATTAAAGTTTc < 1:96040/141‑1 (MQ=255) ggTATTACAATGCACGTAAATCAAAATATGCGACTGGTCGTACACCAAGAAAATTTGCTGTCATGCAATCAACTGCAGGATATTATGGACATGTCGCAGTTGTTGAACAAGTATATAAAAACAGTAGTATTAAAGTTTCAg < 1:241970/141‑1 (MQ=255) tATTACAATGCACGTAAATCAAAATATGCGAATGGTCGTACACCAAGAAAATTTGATGTCATGCAATCAACTGCAGGATATTATGGCCATGTCGCAGTTGTTGAACAAGTATATAAAAACAGTAGTATTAAAGTTTCAGaa < 1:181230/141‑1 (MQ=255) aaTGCTCGTAAATCAAAATATGCGACTGTTCGTACACCAAAAAAATTTGCTGTCATGCAATCAACTGCAGGATATTATGGACATGTCGCAGTTGTTGAACAAGTATATAAAAACAGTAGTATTAAAGTTTCAGAATACAAc < 1:151879/141‑1 (MQ=255) aTGCACGTAAATCAAAATATGCGACTGGTCGTACACCAAGAAAATTTGCTGTCATGCAATCAACTGCAGGATATTATGGACATGTCGCAGTTGTTGAACAAGTATATAAAAACAGTAGTATTAAAGTTTCAGAATACAACt < 1:120320/141‑1 (MQ=255) atatGCGACTGGTCGTACACCAAGAAAATTTGCTGTCATGCAATCAACTGCAGGATATTATGGACATGTCGCAGTTGTTGAACAAGTATATAAAAACAGTAGTATTAAAGTTTCAGAATACAACTTTTATCGCCCATTaaa < 1:277019/141‑1 (MQ=255) tGGTCGTACACCAAGAAAATTTGCTGTCATGCAATCAACTGCAGGATATTATGGACATGTCGCAGTTGTTGAACAAGTATATAAAAACAGTAGTATTAAAGTTTCAGAATACAACTTTTATCGCCCATTAAaatacaatac < 2:164092/141‑1 (MQ=255) tACACCAAGAAAATTTGCTGTCATGCAATCAACTGCAGGATATTATGGACATGTCGCAGTTGTTGAACAAGTATATAAAAACAGTAGTATTAAAGTTTCAGAATACAACTTTTATCGCCCATTAAAATACAATACACgtgt > 1:217984/1‑141 (MQ=255) aGAAAATTTGCTGTCATGCAATCAACTGCAGGATATTATGGACAGGTCGCAGTTGTTGAACAAGTATATAAAAACGGTAGTATTAAAGTTTCAGAATACAACTTTTATCGCCCATTAAAATACAATACACGTGTACTAAGc < 2:266676/141‑1 (MQ=255) aaaTTTGCGGTCATGCAATCAACTGCAGGATATTATGGACATGTCGCAGTTGTTGAACAAGTATATAAAAACAGTAGTATTAAAGTTTCAGAATACAACTTTTATCGCCCATTAAAATACAATACACGTGTACTAAGCaaa < 1:179197/141‑1 (MQ=255) aTTTGCTGTCATGCAATCAACTGCAGGATATTATGGACATGTCGCAGTTGTTGAACAAGTATATAAAAACGGTAGTATTAAAGTTTCAGAATACAACTTTTATCGCCCATTAAAATACAATACACGTGTACTAAGCaaaaa > 2:75194/1‑141 (MQ=255) ttGCTGTCATGCAATCAACTGCAGGATATTATGGACATGTCGCAGTTGTTGAACAAGTATATAAAAACAGTAGTATTAAAGTTTCAGAATACAACTTTTATCGCCCATTAAAATACAATACACGTGTACTAAGCAAAAAgg < 1:262741/141‑1 (MQ=255) cTGTCATGCAATCAACTGCAGGATATTATGGACATGTCGCAGTTGTTGAACAAGTATATAAAAACAGTAGTATTAAAGTTTCAGAATACAACTTTTATCGCCCATTAAAAGACAATACACGTGTACTAAGCAAAAAGgcag > 2:210764/1‑141 (MQ=255) gCAATCAACTGCAGGATATTATGGACATGTCGCAGTTGTTGAACAAGTATATAAAAACAGTAGTATTAAAGTTTCAGAATACAACTTTTATCGCCCATTAAAATACAATACACGTGTACTAAGCAAAAAGGCAGCACGTaa > 2:38628/1‑141 (MQ=255) aCTGCAGGATATTATGGACATGTCGCAGTTGTTGAACAAGTATATAAAAACAGTAGTATTAAAGTTTCAGAATACAACTTTTATCGCCCATTAAAATACAATACACGTGTACTAAGCAAAAAGGCAGCACGTAACTTTAAc < 2:123025/141‑1 (MQ=255) aCTGCAGGATATTATGGACATGTCGCAGTTGTTGAACAAGTATATAAAAACAGTAGTATTAAAGTTTCAGAATACAACTTTTATCGCCCATTAAAATACAATACACGTGTACTAAGCAAAAAGGCAGCACGTAACTTTAAc < 2:31212/141‑1 (MQ=255) tatGGACATGTCGCAGTTGTTGAACAAGTATATAAAAACAGTAGTATTAAAGTTTCAGAATACAACTTTTATCGCCCATTAAATTACTATACTTTTGTAAAAATTCTTAAGGCAGCGCGTAACTTTAACTCGATTTACTaa < 1:244315/141‑1 (MQ=255) gTCGCAGTTGTTGAACAAGTATATAAAAACAGTCGTATTAAAGTTTAAGAATACAACTTTTCTCGCCCATTAAAATCCAATACACGTGTACTAAGCAAAAAGGCAGCACGTAACTTTAACTATATTTACTAATCAAAAAAc < 1:69769/141‑1 (MQ=255) gCAGTTGTTGAACAAGTATATAAAAACAGTAGTATTAAAGTTTCAGAATACAACTTTTATCGCCCATTAAAATACAATACACGTGTACTAAGCAAAAAGGCAGCACGTAACTTTAACTATATTTACTAATCAAAAAACTTc < 1:6312/141‑1 (MQ=255) gCAGTTGTTGAACAAGTATATAAAAACAGTAGTATTAAAGTTTCAGAATACAACTTTTATCGCCCATTAAAATACAATACACGTGTACTAAGCAAAAAGGCAGCACGTAACTTTAACAATATTTACTAATCAAAAAACTTc > 2:137466/1‑141 (MQ=255) aaCAAGTATATAAAAACAGTAGTATTAAAGTTTCAGAATACAACTTTTATCGCCCATTAAAATACAATACACGTGTACTAAGCAAAAAGGCAGCACGTAACTTTAACTATATTTACTAATCAAAAAACTTCTATCACGAAc > 1:89432/1‑141 (MQ=255) taAAAACAGTAGTATTAAAGTTTCAGAATACAACTTTTATCGCCCATTAAAATACAATACACGTGTACTAAGCAAAAAGGCAGCACGTAACTTTAACTATATTTACTAATCAAAAAACTTCTATCACGAACGCTTCAAttt > 1:163786/1‑141 (MQ=255) | ATGCTAAAAATTGGTATTACAATGCACGTAAATCAAAATATGCGACTGGTCGTACACCAAGAAAATTTGCTGTCATGCAATCAACTGCAGGATATTATGGACATGTCGCAGTTGTTGAACAAGTATATAAAAACGGTAGTATTAAAGTTTCAGAATACAACTTTTATCGCCCATTAAAATACAATACACGTGTACTAAGCAAAAAGGCAGCACGTAACTTTAACTATATTTACTAATCAAAAAACTTCTATCACGAACGCTTCAATTT > CP000730/2679713‑2679980 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |