Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 35,997 T→C 30.9% T185T (ACA→ACG USA300HOU_0026 ← IS431mec transposase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP00073035,9970TC30.9% 365.1 / 259.6 326T185T (ACA→ACGUSA300HOU_0026IS431mec transposase
Reads supporting (aligned to +/- strand):  ref base T (118/105);  new base C (42/58);  total (161/165)
Fisher's exact test for biased strand distribution p-value = 7.25e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.70e-01

Only 100 of 326 total aligned reads displayed.

AGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAGCCATTGCTACC  >  CP000730/35868‑36135
                                                                                                                                 |                                                                                                                                           
aGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACt                                                                                                                                  >  2:2305/1‑141 (MQ=14)
 gtgtTCGTTTAACTTGCTAGCATGATGCTAATTTTGTGGTATGGTGAAAATCCGTATATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACtt                                                                                                                                 >  2:279205/1‑141 (MQ=11)
 gtgtTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACtt                                                                                                                                 <  2:165168/141‑1 (MQ=12)
  tgtTCGTCTAACTTGCTAGCATGATGCTAATTTCGGGGCATGGGTAAACTCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTGTTTGCCGTATTGATACttt                                                                                                                                >  2:235536/1‑141 (MQ=11)
   gtTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTg                                                                                                                               >  1:91551/1‑141 (MQ=17)
   gtTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTg                                                                                                                               >  1:218223/1‑141 (MQ=17)
     tcgtttAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGAt                                                                                                                             >  2:289801/5‑141 (MQ=12)
     tCGTTTAACGTGCTAGCATGATGCTAATTGCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGAt                                                                                                                             <  1:254666/141‑1 (MQ=255)
     tCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGAt                                                                                                                             >  1:34949/1‑141 (MQ=32)
       gCTTGACTGGCTAACATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCGGGCGAGACGAGGGGTTCTTTTTAGATATGGCGTAAATACACTCCATACCTTATAAAGTATTCCTTGCTATCTTGATAcgtggagac                                                                                                                           >  2:18553/1‑133 (MQ=2)
        tttAACTTGCTAGCATGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATatc                                                                                                                          >  1:108439/2‑139 (MQ=11)
         ttAACTTGCTAGCATGATGCTAAGTTCTTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCt                                                                                                                         <  2:260539/141‑1 (MQ=11)
          tAACTTGCTAGCATGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCtt                                                                                                                        >  1:240228/1‑141 (MQ=255)
           aaCTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTg                                                                                                                       >  2:123543/1‑141 (MQ=32)
             cTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGtc                                                                                                                     >  1:278805/1‑141 (MQ=17)
              ttGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGtct                                                                                                                    <  1:196818/141‑1 (MQ=17)
               tGCTTGCATGATGCTAATTTCGTGTCATGGCAAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGtctt                                                                                                                   >  2:331188/1‑141 (MQ=255)
               tGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGtctt                                                                                                                   <  2:257462/141‑1 (MQ=17)
                 cTAGCATGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGtctttc                                                                                                                >  1:132981/1‑141 (MQ=255)
                 cTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGtctttc                                                                                                                <  1:72235/141‑1 (MQ=17)
                        gatgCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACttt                                                                                                         <  2:265513/141‑1 (MQ=25)
                             taagtgcGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAGTGTCTTTTAAAGTCTTCTTTGCTGTCTTTATACTTAGATACTTTGTCTTTCTTACTGTCatat                                                                                                    >  2:168161/7‑141 (MQ=11)
                             tAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAatat                                                                                                    >  2:52750/1‑141 (MQ=25)
                             tAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAatat                                                                                                    >  2:100829/1‑141 (MQ=21)
                              aaTTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGCGACCTGCAGTTCTTTTTATATAGAGCGTTAATACCGCCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATg                                                                                                   <  1:159455/141‑1 (MQ=11)
                                tttCGTGACATGGCGAAAATCCGGAGATCCCAAGAGACCTGCGGATATTTTTATATAGAGCGTAAATACACCCAATACCTTTTACAGTAGTCTGAACTGAATGGATACCTTACTACCTCGTCTTTCTCACttaaccatgcc                                                                                                 >  2:47485/1‑132 (MQ=2)
                                 ttCGTGGCATGGGGAAAATCCGTAGATCTGAATAGACCTACGGTTCTTTTTATCTATAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTTATAATATCAAATCTTGTCTTACTTCCTTTacactgccc                                                                                                >  2:91279/1‑133 (MQ=11)
                                  tCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACgg                                                                                               <  2:299381/141‑1 (MQ=37)
                                   cGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTTAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGt                                                                                              <  1:171511/141‑1 (MQ=255)
                                      ggCATGGGGAAAATCCGTAGATCTGAAGAGCCCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGttag                                                                                           >  2:203015/1‑138 (MQ=11)
                                      ggCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACATTCCATCCATTTTAAAGTTGTCGTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTggt                                                                                           >  2:118692/1‑139 (MQ=11)
                                        cATGGCGAAAACTCGTAGATCTGAAGAGACCCGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCt                                                                                         <  1:119867/141‑1 (MQ=255)
                                          tGGGGAAAATCCGTAAATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTATTATGACGGTGATCTTg                                                                                       <  1:165294/141‑1 (MQ=14)
                                                aaaTCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATTTTGCTCAAt                                                                                 >  2:126221/1‑141 (MQ=255)
                                                aaaTCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTATGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTCGTCAAt                                                                                 >  1:224549/1‑141 (MQ=255)
                                                 aaTCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATg                                                                                <  2:65858/141‑1 (MQ=38)
                                                  aTCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGa                                                                               <  1:137689/141‑1 (MQ=255)
                                                   tCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAg                                                                              <  1:5660/141‑1 (MQ=38)
                                                    ccGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAgg                                                                             >  1:235827/1‑141 (MQ=39)
                                                    ccGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATATGGCTCAATGAgg                                                                             >  1:147573/1‑141 (MQ=255)
                                                    ccGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGccacatacgg                                                                             >  2:21540/1‑132 (MQ=255)
                                                    ccGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAgg                                                                             <  2:247187/141‑1 (MQ=255)
                                                     cGTAGATCTGAAGGGGACTACGCGTCTTTTTATATAGAGCGTAAATACATTTAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTAGCTCGATGAGGt                                                                            <  2:39438/141‑1 (MQ=11)
                                                     cGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGt                                                                            >  1:324879/1‑141 (MQ=38)
                                                     cGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGt                                                                            >  1:111078/1‑141 (MQ=255)
                                                         gATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTAtt                                                                        >  1:321413/1‑141 (MQ=255)
                                                          aTCTGACGCGACCTGCGCTCCTTTTTATATAGACCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTc                                                                       <  1:189798/141‑1 (MQ=14)
                                                          aTCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTc                                                                       >  1:43951/1‑141 (MQ=38)
                                                                 gagaCCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATAtt                                                                <  2:103487/141‑1 (MQ=255)
                                                                  agaCCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATAttt                                                               <  2:150899/141‑1 (MQ=255)
                                                                   gaCCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTATAAAGTATTCTATGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCACTGAGGTTATTCAGATGTTTc                                                              >  2:62123/1‑141 (MQ=11)
                                                                    aCCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGAATTATGTTGCTGAAAATGACGGTGATCTTGCTCAATGCGGTTATTCAGATATTTCg                                                             >  1:326883/1‑141 (MQ=11)
                                                                     ccTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGa                                                            >  1:256429/1‑141 (MQ=38)
                                                                        tcGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGt                                                         <  2:240265/140‑1 (MQ=255)
                                                                        gCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTTTTGTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGt                                                         <  1:75815/141‑1 (MQ=255)
                                                                          ggTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTAc                                                       >  2:254890/1‑141 (MQ=38)
                                                                             tCTTTTTATATAGAGCGTAAATACACTCAACACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTAAATTAGGTTATTCAGATATTTCGATGTACAAt                                                    <  2:56784/141‑1 (MQ=255)
                                                                               tttttATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATgg                                                  <  1:283099/141‑2 (MQ=255)
                                                                                ttgtatataGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTGATTCAGATATTTCGATGTACAATggc                                                 <  2:206403/138‑3 (MQ=255)
                                                                                 tttATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACa                                                <  2:272027/141‑1 (MQ=255)
                                                                                   tatataGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGt                                              <  1:310215/141‑1 (MQ=255)
                                                                                    atataGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTc                                             <  1:275112/141‑1 (MQ=255)
                                                                                      ttaGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAg                                           <  2:309578/140‑1 (MQ=255)
                                                                                       cagactggaaaCACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAgg                                          <  1:155717/133‑1 (MQ=14)
                                                                                        agagCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGgt                                         >  1:280093/1‑141 (MQ=255)
                                                                                        agagCGTAAATACACTCAATACATTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGTTGATCTTGCTCATTGCAGTTATTCAGATATTTCGATGTACAATGGCAGACAGgt                                         >  2:44202/1‑141 (MQ=14)
                                                                                         gagCGTAAATACATTCAATACATTTTATAGGATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGgtt                                        >  2:322392/1‑141 (MQ=255)
                                                                                          agCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATCCTTTGATTCCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGgttt                                       <  1:246192/141‑1 (MQ=255)
                                                                                           gCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGgttta                                      <  1:176410/141‑1 (MQ=255)
                                                                                           gCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGgtt                                        >  2:284054/1‑139 (MQ=255)
                                                                                             ttAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGgtttaag                                    <  1:149773/140‑1 (MQ=255)
                                                                                             gTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGgtttaag                                    <  2:132981/141‑1 (MQ=255)
                                                                                                aaTACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGgtttaagttt                                 >  1:204924/1‑141 (MQ=255)
                                                                                                aaTACACTTAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGgtttaagttt                                 <  2:129071/141‑1 (MQ=255)
                                                                                                aaTACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGAAGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCCGgtttaagttt                                 >  2:145367/1‑141 (MQ=255)
                                                                                                 aTACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGgtttaagttta                                >  1:169799/1‑141 (MQ=255)
                                                                                                  tacacttaaTACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTTTTTCTTACTTTAATATGACGGTGATCTTGCTCTATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGgtttaagtttaa                               >  2:209707/8‑141 (MQ=14)
                                                                                                  tACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTCATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGgtttaagtttaa                               <  2:222816/141‑1 (MQ=255)
                                                                                                  tACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGgtttaagtttaa                               >  1:102723/1‑141 (MQ=255)
                                                                                                  tACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCCGTCAGgtttaagtttaa                               >  1:4759/1‑141 (MQ=255)
                                                                                                      ctCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGCGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTATaaaact                           >  2:163233/2‑139 (MQ=255)
                                                                                                      ctCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGc                           >  1:232559/2‑141 (MQ=255)
                                                                                                       tCAATACCTTTTAAAGTACACTACAATTTATTTATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCt                          >  2:153061/1‑141 (MQ=14)
                                                                                                         aaTACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTATTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCttt                        >  2:19054/1‑141 (MQ=255)
                                                                                                           tACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTaa                      <  1:261854/141‑1 (MQ=255)
                                                                                                           tACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTATTATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTTAAAGCTTTaa                      >  1:298913/1‑141 (MQ=255)
                                                                                                            aCCTTTTAATGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAAt                     <  2:278624/141‑1 (MQ=255)
                                                                                                            aCCTTTTAAAGTATTCTTTTCTGTATTGATACTTTGATACTTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCTGATAGTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAAt                     <  2:170636/141‑1 (MQ=14)
                                                                                                             ccTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAAtt                    >  1:123639/1‑141 (MQ=255)
                                                                                                                 ttAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACtt                >  1:214394/1‑141 (MQ=255)
                                                                                                                 ttAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTACtt                <  1:57237/141‑1 (MQ=40)
                                                                                                                 ttAAAGTATTCTTTGCCGTATTGATACTGTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTattt                <  1:6680/141‑4 (MQ=255)
                                                                                                                    aaGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTaa             <  1:113023/141‑2 (MQ=255)
                                                                                                                        aTTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGTTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCAt         >  2:315579/1‑141 (MQ=255)
                                                                                                                        aTTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTCCTTTAACCAt         >  1:302762/1‑141 (MQ=255)
                                                                                                                         ttCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCAtt        <  1:296928/141‑1 (MQ=255)
                                                                                                                          tCTTCGCTGAATCGTTACTTTGATACCTTGGTTTTTTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATTTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTg       <  1:193257/141‑1 (MQ=14)
                                                                                                                            ttttCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATACTTCGCTGTACAATGGCCGTCAGGTTTAAGTTTAAAAGCTTTACTTACTTTAACCATTGCt     <  2:138666/141‑1 (MQ=14)
                                                                                                                            tttGCTGTATTGATACTTACATCCCTTGTCTTTCTTACGTTAATATGACGGTGCTCTTGCTCAATGAGGTTATTCAGATATTCCGATGTACAACTTCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCt     <  1:28984/141‑1 (MQ=11)
                                                                                                                              tGCTGTCTTGATACTTTTATCCCTTGTCGTTCTTACTTTAATACGACGGTGATCTTGCTCAATGAGGTTAGTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTAc   <  2:175989/141‑1 (MQ=14)
                                                                                                                                 |                                                                                                                                           
AGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAGCCATTGCTACC  >  CP000730/35868‑36135

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: