Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 1,929,843 Δ1 bp 61.8% intergenic (‑926/‑242) USA300HOU_1794 ← / → USA300HOU_1796 hypothetical protein/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007301,929,8430A.61.8% 159.2 / inf 183intergenic (‑926/‑242)USA300HOU_1794/USA300HOU_1796hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (35/33);  new base . (62/51);  total (98/85)
Fisher's exact test for biased strand distribution p-value = 7.58e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 183 total aligned reads displayed.

CTCATAGAATTTGTCCAATTAAGTGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCG‑TCAATTTAGACGCAGAG‑AGGAGGTG‑TA‑T‑AA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACATATAGCCGCA  >  CP000730/1929704‑1929978
                                                                                                                                            |                                                                                                                                              
ctcATAGAATTTGTCCAATTAAGTGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCG‑TCAATTTAg                                                                                                                                               >  1:668581/1‑141 (MQ=255)
  cATAGAATTTGTCCAATTAAGTGTAGACGATTCAAACCATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCG‑TCAATTTAGAc                                                                                                                                             <  1:394381/141‑1 (MQ=255)
     aGAATTTGTCCAATTAAGTGTAGACGATTCAATCTATCAGCGAGAATCATATAACTTATCAGAGGCAAGTGACTGTGCTTGTATATTTGCCGATGAGATAATATTGGAGTCGCCTATCTCTAAGGCG‑TCAATTGAGCCGCa                                                                                                                                          >  2:396959/1‑141 (MQ=255)
      gAATTTGTCCAATTAAGTGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCG‑TCAATTTAGACGCag                                                                                                                                         <  2:692173/141‑1 (MQ=255)
           tGTCCAATTAAGTGTAGACGATTCAATCTATCAGCTAGAATCACATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCG‑TCAATTTAGACGCAGAg‑agg                                                                                                                                   <  1:66082/141‑1 (MQ=255)
              ccAATTAAGTGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCG‑TCAATTTAGACGCAGAG‑aggagg                                                                                                                                >  1:539066/1‑141 (MQ=255)
               cAATTAAGTGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCG‑TCAATTTAGACGCAGAG‑AGGAGgt                                                                                                                               <  2:175467/141‑1 (MQ=255)
               cAATTAAGTGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCG‑TCAATTTAGACGCAGAG‑AGGAGgt                                                                                                                               <  2:417259/141‑1 (MQ=255)
               cAATAAAGTGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCG‑TCAATTTAGACGCAGAG‑AGGAGgt                                                                                                                               <  2:25293/141‑1 (MQ=255)
                aaTTAAGTGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCG‑TCAATTTAGACGCAGAG‑AGGAGgtg                                                                                                                              <  2:156881/141‑1 (MQ=255)
                     aGTGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCGGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCG‑CTAATTTAGACGCAGAG‑AGGAGGTG‑TA‑T‑aa                                                                                                                      >  1:437386/1‑141 (MQ=255)
                       tgtAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCG‑TCAATTTAGACGCAGAG‑AGGAGGTG‑TA‑T‑AA‑g‑g                                                                                                                  <  1:601325/141‑1 (MQ=255)
                        gtAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCG‑TCAATTTAGACGCAGAG‑AGGAGGGG‑TA‑T‑AA‑g‑gg                                                                                                                 >  1:246895/1‑140 (MQ=255)
                             cGATTCAATCTATCGGCTAAAATCAAATAACTTATCAGAAGCAACTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCG‑TCAATTTAGACGCAGAG‑AGGAGGTG‑TA‑T‑AA‑G‑G‑TGATGc                                                                                                           >  1:423686/1‑141 (MQ=255)
                             cGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCG‑TCAATTTAGACGCAGAG‑AGGAGGTG‑TA‑T‑AA‑G‑G‑TGATGc                                                                                                           >  1:386619/1‑141 (MQ=255)
                             cGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCA‑TAAATGCAGACGCAGAG‑AGGAGGGG‑TA‑C‑AA‑G‑G‑TGATGc                                                                                                           >  1:478470/1‑141 (MQ=255)
                                      cTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCG‑TCAATTTAGACGCAGAG‑AGGAGGTG‑TA‑T‑AA‑G‑G‑TGATGCTTATTTTCg                                                                                                  <  1:534176/141‑1 (MQ=255)
                                       tATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCG‑TCAATTTAGACGCAGAG‑AGGAGGTG‑TA‑T‑AA‑G‑G‑TGATGCTTATTTTCGt                                                                                                 >  2:624848/1‑141 (MQ=255)
                                       tATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCG‑TCAATTTAGACGCAGAG‑AGGAGGTG‑TA‑T‑AA‑G‑G‑TGATGCTTATTTTCGt                                                                                                 >  1:32723/1‑141 (MQ=255)
                                                    cATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGCTGATATAATAGTAGAGTCGCCTATCTCTCAGGCG‑TCAATTTAGACGCAGAG‑AGTAGGTG‑TA‑T‑AA‑G‑G‑TGCTGCTTATTTTCGTTCACATCATAGCa                                                                                    >  1:251606/1‑141 (MQ=255)
                                                           cTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCG‑TCAATTTAGACGCAGAG‑AGGAGGTG‑TA‑T‑AA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGtca                                                                             >  2:643536/1‑141 (MQ=255)
                                                                  atcggaagtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCG‑TCAATT‑‑GACGAAGAG‑AGGAGGTG‑CATT‑AC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGc                                                                     >  2:561683/15‑141 (MQ=255)
                                                                  atcggaagtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCG‑TCAATT‑‑GACGAAGAG‑AGGAGGTG‑CATT‑AC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGc                                                                     <  1:117662/127‑1 (MQ=255)
                                                                     ggaagtagatacgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCG‑TCAATT‑‑GACGAAGAG‑AGGAGGTG‑CA‑T‑TACT‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCtgt                                                                  <  2:505844/129‑1 (MQ=255)
                                                                      gaagtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCG‑TCAATT‑‑GACGAAGAG‑AGGAGGTG‑CATT‑AC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCtgtg                                                                 >  2:606465/11‑141 (MQ=255)
                                                                      gaagtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGCCGCCTATCTCTCAGGCG‑TCAATT‑‑GACGAAGAG‑AGGAGGTG‑CATT‑AC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCtgtg                                                                 <  2:134865/131‑1 (MQ=255)
                                                                       aattagatatgCCTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCG‑TCAATT‑‑GACGAAGAG‑AGGAGGTG‑CA‑T‑TA‑CTT‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGc                                                                <  2:555817/132‑1 (MQ=255)
                                                                       aaGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCG‑TCAATTTAGACGCAGAG‑AGGAGGTG‑TA‑T‑AA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCtgtg                                                                 <  2:446337/141‑1 (MQ=255)
                                                                        agtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCG‑TCAATT‑‑GACGAAGAG‑AGGAGGTG‑CATT‑AC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGct                                                               >  1:613140/9‑140 (MQ=255)
                                                                        actagatacgCTTGTATATTCACAGACGGTATAATAATAGAGTTGCCTATCTCTCAGGCG‑TCAATT‑‑GACGAAGAG‑AGGAGGTG‑CA‑T‑TA‑C‑TTTGCTAATCATTTTCGTTCCCATCATAGCACCAGTCATCAGTGGCTGTGct                                                               <  1:192797/132‑2 (MQ=255)
                                                                         gTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCG‑TCAATTTAGACGCAGAG‑AGGAGGTG‑TA‑T‑AA‑G‑G‑TGAGGCGGGTTTTCGTTCACATCATGGCACCAGTCATCAGTGGCTGTGcc                                                               >  1:524609/1‑141 (MQ=255)
                                                                          tagatatgCTTGTATATTCACAGATGGTATAAGAATAGAGTCGCCTATCTCTCAGGCG‑TCAATT‑‑GACGAAGAG‑AGGAGGTG‑CATT‑AC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGctgt                                                             <  2:464364/135‑4 (MQ=255)
                                                                           agatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCG‑TCAATT‑‑GACGAAGAG‑AGGAGGTG‑CATT‑AC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGctgtt                                                            >  1:550046/6‑137 (MQ=255)
                                                                            gatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCG‑TCAATT‑‑GACGAAGAG‑AGGAGGTG‑CA‑T‑TA‑CTT‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGctgttg                                                           >  1:646252/5‑136 (MQ=255)
                                                                            aCTGTGCTTGTATATTTGCCTATGATATAATAGTAGAGTCGCCTATCTCTCAGGCG‑TCAATTTAGACGGAGAG‑AGGAGGTG‑TA‑T‑AA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCAtt                                                            <  2:315977/141‑1 (MQ=255)
                                                                             atatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCG‑TCAATT‑‑GACGAAGAG‑AGGAGGTG‑CATT‑AC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGctgttgc                                                          <  1:315760/138‑7 (MQ=255)
                                                                              tgtgCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCG‑TCAATTTAGACGCAGAG‑AGGAGGTG‑TA‑T‑AA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGc                                                          <  1:60674/141‑1 (MQ=255)
                                                                              tatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCG‑TCAATT‑‑GACGAAGAG‑AGGAGGTG‑CATT‑AC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGAGCTGTTGCg                                                         <  1:567487/139‑1 (MQ=255)
                                                                              tatgCTTGTATATGCACAGATGGTAAAATAATAGAGTCGCCTATCTCTCAGGCG‑TCAATT‑‑GACGAAGAG‑AGGAGGTG‑CATT‑AC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCg                                                         <  1:698596/139‑1 (MQ=255)
                                                                               gtgCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCG‑TCAATTTAGACGCAGAG‑AGGGGGCG‑GA‑C‑AA‑G‑C‑AGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATGGCg                                                         >  1:177912/1‑141 (MQ=255)
                                                                               gtgCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCT‑AAAGAAGCAAAAGTGAGCCG‑AGTGAACGTCGAG‑TA‑T‑AA‑G‑G‑TGATGCTTGTTTTCGTTCACATCAGAGCACCAGTCATCAGTGGCTGTACCATTGCg                                                         >  1:370522/1‑141 (MQ=255)
                                                                               atgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCG‑TCAATT‑‑GACGAAGAG‑AGGAGGTG‑CATT‑AC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGt                                                        <  1:105831/140‑1 (MQ=255)
                                                                                  cTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCG‑TCAATT‑‑GACGAAGAG‑AGGAGGTG‑CATT‑AC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGtatt                                                     >  2:642691/1‑141 (MQ=255)
                                                                                     gTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCG‑TCAATT‑‑GACGAAGAG‑AGGAGGTG‑CATT‑AC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGtattata                                                  <  1:538346/141‑7 (MQ=255)
                                                                                        tattcacagatggtATAATAATAGAGTCGCCTATCTCTCAGGCG‑TCAATT‑‑GACGAAGAG‑AGGAGGTG‑CA‑T‑TA‑C‑TTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACtt                                               >  1:1491/9‑141 (MQ=255)
                                                                                        tatTTACAGATGGTGTAATAATAGAGTCGCCTATCTCTCAGGCG‑TCAATT‑‑GACGAAGAG‑AGGAGGTG‑CATT‑AC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGtattatactt                                               <  1:198709/141‑7 (MQ=255)
                                                                                         attcacagatggtATAATAATAGAGTCGCCTATCTCTCAGGCG‑TCAATT‑‑GACGAAGAG‑AGGAGGTG‑CA‑T‑TACT‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCCGTCATCAGTGGCTGTGCTGTTGCGTATTATACttt                                              >  2:170795/8‑140 (MQ=255)
                                                                                          tttGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCA‑TCAATTTAGACGCAGAG‑ACACGGTG‑TA‑T‑AA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCACCAGTGACTGTTCCCTTGCGtttttttactt                                              >  2:174548/1‑137 (MQ=255)
                                                                                          ttcacagatgatATAAAAATAGAGTCGCCTATCTCTCAGGCG‑TCAATT‑‑GACGAAGAG‑AGGAGGTG‑CATT‑AC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTAt                                             <  2:23651/135‑1 (MQ=255)
                                                                                           tcacagatggtATAATAATAGAGTCGCCTATCTCTCAGGCG‑TCAATT‑‑GACGAAGAG‑AGGAGGTG‑CATT‑AC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTAtt                                            >  1:736519/6‑141 (MQ=255)
                                                                                             acagatggtATAATAATAGAGTCGCCTATCTCTCAGGCG‑TCAATT‑‑GACGAAGAG‑AGGAGGTG‑CATT‑AC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTgg                                          >  1:687411/4‑141 (MQ=255)
                                                                                             acagatgggataataataGAGTCGCCTATCTCTCAGGCG‑TCAATT‑‑GACGAAGAG‑AGGAGGTG‑CA‑T‑TA‑C‑TTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTgg                                          <  1:71719/132‑1 (MQ=255)
                                                                                              cagatggtATAATAATAGAGAAGCATAACTCTCTTACG‑TCAATT‑‑GACGAAGAG‑AGGAGGTG‑CATT‑AC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGc                                         >  1:767767/3‑141 (MQ=255)
                                                                                               agatggtATAATAATAGAGTCGCCTATCTCTCAGGCG‑TCAATT‑‑GACGAAGAG‑AGGAGGTG‑CATT‑AC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCt                                        >  2:211307/2‑141 (MQ=255)
                                                                                                gatggtATAATAATAGAGTCGCCTATCTCTCAGGCG‑TCAATT‑‑GACGAAGAG‑AGGAGGTG‑CATT‑AC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCtt                                       <  1:165398/141‑2 (MQ=255)
                                                                                                  tggtataATAATAGAGTCGCCTATCTCTCAGGCG‑TCAATT‑‑GACGAAGAG‑AGGAGGTG‑CC‑T‑TACT‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAg                                     >  1:297551/4‑141 (MQ=255)
                                                                                                  tggtataATAATAGAGTCGCCTATCTCTCAGGCG‑TCAATT‑‑GACGAAGAG‑AGGAGGTG‑CATT‑AC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAg                                     >  1:324897/4‑141 (MQ=255)
                                                                                                  gggtataATAAGAGAGTCGCCTATCTCCCAGGCG‑TCAATT‑‑GACGAAGAG‑AGGAGGTG‑CATT‑AC‑T‑T‑TGCGAATCATTTTCGTTCACATCATAGCCCCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCttag                                     <  1:261843/138‑4 (MQ=255)
                                                                                                   ggtataATAATAGAGTCGCCTATCTCTCAGGCG‑TCAATT‑‑GACGAAGAG‑AGGAGGTG‑CATT‑AC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGt                                    >  1:409270/3‑141 (MQ=255)
                                                                                                   ggtataATAATAGAGTCGCCTATCTCTCAGGCG‑TCAATT‑‑GACGAAGAG‑AGGAGGTG‑CATT‑AC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGt                                    >  1:582952/3‑141 (MQ=255)
                                                                                                     tataATAGTAGAGTCGCCTATCTCTCAGGCG‑TCAATTTAGACGCAGAG‑AGGAGGTG‑TA‑T‑AA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTa                                   >  2:697637/1‑141 (MQ=255)
                                                                                                     tataATAATAGAGTCGCCTATCTCTCAGGCG‑TCAATT‑‑GACGAAGAG‑AGGAGGTG‑CA‑T‑TACT‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTaa                                  >  2:668643/1‑140 (MQ=255)
                                                                                                     tataATAATAGAGTCGCCTATCTCTCAGGCG‑TCAATT‑‑GACGAAGAG‑AGGAGGTG‑CA‑T‑TACT‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTaa                                  >  2:579148/1‑140 (MQ=255)
                                                                                                      ataataataGAGTCGCCTATCTCTCAGGCG‑TCAATT‑‑GACGAAGAG‑AGGAGGTG‑CATT‑AC‑T‑T‑TGCTACTCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTaaa                                 <  2:576585/141‑3 (MQ=255)
                                                                                                      ataataataGAGTCGCCTATCTCTCAGGCG‑TCAATT‑‑GACGAAGAG‑AGGAGGTG‑CATT‑AC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGATTAGtcaa                                 >  2:465716/1‑138 (MQ=255)
                                                                                                       aaataatAGAGTCGCCTATCTCTCAGGCG‑TCAATT‑‑GACGAAGAG‑AGGAGGTG‑CA‑T‑TA‑C‑TTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTaaac                                <  2:386510/140‑4 (MQ=255)
                                                                                                         ataataGAGTCGCCTATCTCTCAGGCG‑TCAATT‑‑GACGAAGAG‑AGGAGGTG‑CATT‑AC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGc                              <  1:293933/141‑1 (MQ=255)
                                                                                                         ataGTAGAGTCGCCTATCTCTCAGGCG‑TCAATTTAGACGCAGAG‑AGGAGGTG‑TA‑T‑AA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACg                               <  1:247138/141‑1 (MQ=255)
                                                                                                         agtatagAGTCGCCTATCTCTCAGGCG‑TCAATT‑‑GACGAAGAG‑AGGAGGTG‑CATT‑AC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGc                              <  1:632896/137‑1 (MQ=255)
                                                                                                           aatagATTCGCCTATCTCTCAGGCG‑TCAA‑‑TGGACTAAGAG‑AGGAGGTG‑CATT‑AC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaa                            <  2:770001/139‑1 (MQ=255)
                                                                                                           aatagAGTCGCCTATCTCTCAGGCG‑TCAATT‑‑GACGAAGAG‑AGGAGGTG‑CA‑T‑TACT‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaa                            <  2:749139/139‑1 (MQ=255)
                                                                                                             tagAGTCGCCTATCTCTCAGGCG‑TCAATT‑‑GACGAAGAG‑AGGAGGTG‑CATT‑AC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaaaa                          >  1:31180/1‑139 (MQ=255)
                                                                                                             tagAGTCGCCTATCTCTCAGGCG‑TCAATT‑‑GACGAAGAG‑AGGAGGTG‑CA‑T‑TACT‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaaca                          >  2:758991/1‑139 (MQ=255)
                                                                                                             tagAGTCGCCTATCCCTCAGGCG‑TCAATT‑‑GACGAAGAG‑AGGAGGTG‑CATT‑AC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaaca                          <  2:264399/141‑3 (MQ=255)
                                                                                                              agagTCGCCTATCTCTCAGGCG‑TCAATT‑‑GACGAAGAG‑AGGAGGTG‑CATT‑AC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaa                         <  1:634147/141‑4 (MQ=255)
                                                                                                               gagTCGCCTATCCCTCAGGCG‑TCAATTTAGACGCAGAG‑AGGAGGTG‑TA‑T‑AA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATAc                         <  2:41598/141‑1 (MQ=255)
                                                                                                                agTCGCCTATCTCTCAGGCG‑TCAATT‑‑GACGAAGAG‑AGGAGGTG‑CATT‑AC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaat                       >  2:72282/1‑136 (MQ=255)
                                                                                                                agTCGCCTATCTCTCAGGCG‑TCAATT‑‑GACGAAGAG‑AGGAGGTG‑CA‑T‑TACT‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaat                       >  2:480994/1‑136 (MQ=255)
                                                                                                                 gTCGCCTATCTCTCAGGCG‑TCAATTTAGACGCAGAG‑AGGAGGTG‑TA‑T‑AA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACaa                       <  1:492273/141‑1 (MQ=255)
                                                                                                                 gTCGCCGATCTCTCAGGCG‑TCAATT‑‑GAAGAAGAG‑AGGAGGTGCGT‑T‑AC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaata                      >  2:196975/1‑135 (MQ=255)
                                                                                                                    gCCTATCTCTCAGGCG‑TCAATT‑‑GACGAAGAG‑AGGAGGTG‑CATT‑AC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCCCCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTACACGCaacaaatgatt                   >  1:244536/1‑132 (MQ=255)
                                                                                                                    gCCTATCTCTCAGGCG‑TCAATT‑‑GACGAAGAG‑AGGAGGTG‑CATT‑AC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataatt                   >  1:287339/1‑132 (MQ=255)
                                                                                                                    gCCTATCTCTCAGGCG‑TCAATT‑‑GACGAAGAG‑AGGAGGTG‑CATT‑AC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataatt                   <  2:231325/141‑10 (MQ=255)
                                                                                                                    gCCTATCTCTCAGGCG‑TCAATT‑‑GACGAAGAG‑AGGAGGTG‑CA‑T‑TACT‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataatt                   <  2:155011/141‑10 (MQ=255)
                                                                                                                    gCCTATCTCTAAGGCG‑TCAATT‑‑GACGAAGAG‑AGGAGGTG‑CATT‑AC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataatt                   <  2:284388/141‑10 (MQ=255)
                                                                                                                     ccTATCTCTCAGGCG‑TCAATT‑‑GACGAAGAG‑AGGAGGTG‑CC‑TCAC‑G‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCACCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataataa                  >  1:730734/1‑131 (MQ=255)
                                                                                                                     ccTATCTCTCAGGCG‑TCAATT‑‑GACGAAGAG‑AGGAGGTG‑CATT‑AC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataatta                  >  1:491941/1‑131 (MQ=255)
                                                                                                                     ccTATCTCTCAGGCG‑TCAATT‑‑GACGAAGAG‑AGGAGGTG‑CA‑T‑TACT‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataatta                  >  1:457055/1‑131 (MQ=255)
                                                                                                                      cTATCTCTCAGGCG‑TCAATT‑‑GACGAAGAG‑AGGAGGTG‑CATT‑AC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataattaa                 >  1:320741/1‑130 (MQ=255)
                                                                                                                      cTATCTCTCAGGCG‑TCAATT‑‑GACGAAGAG‑AGGAGGTG‑CATT‑AC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataattaa                 >  2:101946/1‑130 (MQ=255)
                                                                                                                      cTATCTCTCAGGCG‑TCAATT‑‑GACGAAGAG‑AGGAGGTG‑CATT‑AC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataattaa                 >  2:85138/1‑130 (MQ=255)
                                                                                                                      cTATCTCTCAGGCG‑TCAATT‑‑GACGAAGAG‑AGGAGGTG‑CATT‑AC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataattaa                 >  1:744668/1‑130 (MQ=255)
                                                                                                                       tATCTCTCAGGCG‑TCAATT‑‑GTCGAAGAG‑AGGAGGTG‑CA‑T‑TA‑CTT‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataattaat                <  2:71437/141‑13 (MQ=255)
                                                                                                                       tATCTCTCAGGCG‑TCAATT‑‑GACGAAGAG‑AGGAGGTG‑CATT‑AC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataattaat                <  2:613140/141‑13 (MQ=255)
                                                                                                                       tATCTCTCAGGCG‑TCAATT‑‑GACGAAGAG‑AGGAGGTG‑CATT‑AC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataattaaa                >  1:694320/1‑129 (MQ=255)
                                                                                                                        aTCTCTCAGGCG‑TCAATT‑‑GACGAAGAG‑AGGAGGTG‑CA‑T‑TA‑C‑TTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataattaata               >  2:666719/1‑128 (MQ=255)
                                                                                                                         tctctcAGGCG‑TCAATTTAGACGCAGAG‑AGGAGGTG‑TA‑T‑AA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTTTTTTTTTTTAAGTAGACGCAATACAAAATAGGTg               >  2:205758/1‑141 (MQ=255)
                                                                                                                             ccAGGCG‑GCAATTTAGACGCAGAG‑AGGAGGGG‑TA‑T‑AA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTAGTGGCTAAGTAGACGCAATACAAAATAGGTGACat           <  1:342103/140‑1 (MQ=255)
                                                                                                                               aGGCG‑TCAATTTAGACGCAGAG‑AGGAGGGG‑TA‑T‑AA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGGGGCatat         >  2:587540/1‑141 (MQ=255)
                                                                                                                                       aaTTTAGACGCAGAG‑AGGAGGTG‑TA‑T‑AA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATTCAAAATAGGTGAGATATAGCCGCa  >  2:341349/1‑141 (MQ=255)
                                                                                                                                            |                                                                                                                                              
CTCATAGAATTTGTCCAATTAAGTGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCG‑TCAATTTAGACGCAGAG‑AGGAGGTG‑TA‑T‑AA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACATATAGCCGCA  >  CP000730/1929704‑1929978

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑