Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 35,997 T→C 29.1% T185T (ACA→ACG USA300HOU_0026 ← IS431mec transposase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP00073035,9970TC29.1% 867.9 / inf 738T185T (ACA→ACGUSA300HOU_0026IS431mec transposase
Reads supporting (aligned to +/- strand):  ref base T (256/266);  new base C (116/98);  total (373/365)
Fisher's exact test for biased strand distribution p-value = 2.23e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.11e-01

Only 100 of 738 total aligned reads displayed.

TCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAGCCATTGCTACCT  >  CP000730/35861‑36136
                                                                                                                                        |                                                                                                                                            
tcttgtgAGTGTTCGTTTAATTTCCTAGCATGATGCTAATTTCGTGGCCTGGCGAAAATCCGTAGATCTGAAGAGACTTGCGGTTCTTTTTATACAGCGCGTGAAGACACTCAATACCTTTCAAAGTATTCTTTGCTGTAt                                                                                                                                          <  2:364006/138‑1 (MQ=1)
       aGTGTTCGTTTTACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACt                                                                                                                                   <  2:148783/141‑1 (MQ=9)
       aGTGTTCGTTTAACTAGCTGGCATGATGCTAATTTCGTGGCACGGCGAAAATCCGTAGATCTGATGAGACCTGCCGTTCTTTTTATATAAAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACt                                                                                                                                   >  1:577575/1‑141 (MQ=11)
       aGTGGTCGTTTAGCTTGCTAGCATGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATAGATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACt                                                                                                                                   >  1:546951/1‑141 (MQ=11)
        gtgtTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACtt                                                                                                                                  >  1:271978/1‑141 (MQ=17)
         ggtTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACttt                                                                                                                                 <  1:34528/140‑1 (MQ=12)
          gtTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTg                                                                                                                                <  1:635484/141‑1 (MQ=17)
           ttCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGa                                                                                                                               >  1:144662/1‑141 (MQ=17)
             cGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATa                                                                                                                             >  1:39537/1‑141 (MQ=32)
              gtttAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATAc                                                                                                                            <  2:434122/139‑1 (MQ=17)
               tttAACTTGCGAGCATGACGCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATatc                                                                                                                           <  2:555603/140‑3 (MQ=11)
               cTTAACTTGCTAGCATGTTGGTAAGTTCTGTGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATAcc                                                                                                                           <  2:540537/141‑1 (MQ=11)
                ttAACTTGCTAGCATGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACTGTTCTTTTTATATAGAGCGTAAAGACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCt                                                                                                                          <  2:340342/141‑1 (MQ=11)
                    cTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTCTTGATACTTTGATACCTTGtc                                                                                                                      >  1:53299/1‑141 (MQ=17)
                    cTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAGAGTATTCTTTGCTGTATTGATACTTTGATACCTTGtc                                                                                                                      >  2:223914/1‑141 (MQ=17)
                    cTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGtc                                                                                                                      <  2:251446/141‑1 (MQ=17)
                      tGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGtctt                                                                                                                    >  2:330751/1‑141 (MQ=17)
                       gCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGtcttt                                                                                                                   <  2:664539/141‑1 (MQ=17)
                        cTAGCATGATGCTAATTTCGTGGCATGGGGAAAAGCCGTAGAACTCAAGAGACGTACGGTTCTTTTGATATAGAGCGTAAATGCATGCAATACCTTTTAAAGCATTCTTTGCCGCAGTGATACTGTGATATCTTgcgttgt                                                                                                                  >  2:113780/1‑135 (MQ=11)
                        cTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGtctttt                                                                                                                  >  1:724083/1‑140 (MQ=17)
                         tAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCCATACCTTTTAAAGTATTCTTTGTTGTATTGATACTTTGATACCTTGtttttct                                                                                                                 >  1:91447/1‑141 (MQ=255)
                          aGCATGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGtctttctt                                                                                                                <  1:68374/141‑1 (MQ=255)
                          aGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTCAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGtctttctt                                                                                                                >  1:597208/1‑141 (MQ=17)
                           gCATGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATGCTTTGATATCTTGTCTTTCTTa                                                                                                               >  1:168222/1‑141 (MQ=11)
                           gCATGATGCTAATTTCATGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGCTACTTTGATACCTTGtctttgttt                                                                                                               >  1:382374/1‑140 (MQ=255)
                              tgatgCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACtt                                                                                                            >  2:313299/1‑141 (MQ=255)
                              tgatgCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACtt                                                                                                            <  1:427471/141‑1 (MQ=25)
                                     aaTTTCGTGGCATGGCGAAAATCCGTATATCTCAAGGTACCTGCGGTCCTTTTTATATTGAGCGTAAATATATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATg                                                                                                     <  2:463112/141‑1 (MQ=11)
                                        ttCGTGGCATGGCGAAAATCCGTATATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTTATACCTTGTCTCTCTTACTTTAATATGCCg                                                                                                  >  1:60573/1‑141 (MQ=11)
                                          tgTGGCATGGCGAAAATCCGGAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTTTCTTTCTTACTTTAATATGACGGt                                                                                                <  1:652504/140‑1 (MQ=255)
                                          cGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGt                                                                                                <  2:651099/141‑1 (MQ=37)
                                            gggCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGa                                                                                              <  1:100253/140‑1 (MQ=255)
                                             ggCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGAt                                                                                             <  1:776865/141‑1 (MQ=255)
                                             ggCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGAt                                                                                             >  1:258789/1‑141 (MQ=255)
                                             ggCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGAt                                                                                             >  2:726129/1‑141 (MQ=38)
                                                     gAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCa                                                                                    >  2:275121/1‑141 (MQ=255)
                                                      aaaaTCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCaa                                                                                   <  1:130817/141‑1 (MQ=255)
                                                        aaTCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATg                                                                                 >  1:282232/1‑141 (MQ=255)
                                                           ccGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAgg                                                                              <  1:238922/141‑1 (MQ=255)
                                                           ccGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAgg                                                                              <  2:39630/141‑1 (MQ=38)
                                                           ccGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAgg                                                                              <  2:763292/141‑1 (MQ=255)
                                                           ccGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAgg                                                                              <  1:404685/141‑1 (MQ=38)
                                                           ccGTAGATCTGAAGAGACCTACGGATCTTTTTATATAGAGCGTACATACATTCCCTACCTTTTACAGTACTCTACGCCGTATTGATACTTAGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAgg                                                                              >  2:181554/1‑141 (MQ=11)
                                                            cGTAGATCTGAAGAGTCCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCACTACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATAACTTGTCTTTCTTACTTTAATATGACGTTGATCTTGTTAAATAAGGt                                                                             >  1:49288/1‑141 (MQ=11)
                                                            cGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGGGGt                                                                             >  1:316125/1‑141 (MQ=38)
                                                            cGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGt                                                                             >  1:669348/1‑141 (MQ=37)
                                                            cGTAGATCTGAAGAAAGCTGCGGTTCATAACATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGt                                                                             >  2:585556/1‑141 (MQ=11)
                                                             gTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATTTGACGGTGATCTTGCTCAATGAGGtt                                                                            >  1:416909/1‑141 (MQ=255)
                                                               tgATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGGCGTATTGATCCGGGGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTAt                                                                          <  1:180602/140‑1 (MQ=14)
                                                                         agaCCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCCATGAGGTTATTCAGATAttt                                                                >  1:479788/1‑141 (MQ=255)
                                                                           aCCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCg                                                              <  2:380022/141‑1 (MQ=38)
                                                                            cctacGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGa                                                             >  1:358838/5‑141 (MQ=255)
                                                                            ccTGCGGTTCTTTTTAGTTATAGCGTAAAGACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTACTATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGa                                                             <  1:336836/141‑1 (MQ=11)
                                                                                cGGTTCTTTTTATATAGAGCGTAAATACACTGAATACCTTTTCAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGCCGGTGATCTTGCTCAATGACGTTATTCCGATACATCGCTGTa                                                         >  2:624885/1‑141 (MQ=11)
                                                                                    tCTGTTTATATAGAGCGTAAATACATTCAATCCCTTTTAGAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGAGCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAAt                                                     <  2:21021/141‑1 (MQ=255)
                                                                                        tttATATTGAGCTTAAATCCCCTCAATACCTTTTAAAGTATGCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATCTGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCa                                                 <  2:484769/141‑1 (MQ=11)
                                                                                         ttATATAGAGCGTAAAAACCAACGATGCCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAg                                                >  2:355909/1‑141 (MQ=14)
                                                                                            tataGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTCCTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCa                                             >  1:295709/1‑141 (MQ=255)
                                                                                             ataGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAg                                            >  1:509129/1‑141 (MQ=255)
                                                                                              taGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAgg                                           <  1:765570/141‑1 (MQ=255)
                                                                                               agagCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGgt                                          >  1:218452/1‑141 (MQ=255)
                                                                                                gagCGTAGATACAGTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGgtt                                         >  2:669933/1‑141 (MQ=255)
                                                                                                gagCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATGTCGATGTACAATGGCAGTCAGgtt                                         >  2:504491/1‑141 (MQ=255)
                                                                                                 agCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGgttt                                        >  1:64331/1‑141 (MQ=255)
                                                                                                     tAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATCTGACACCACGTTGTCGCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGgtttaagt                                    >  1:761018/1‑141 (MQ=11)
                                                                                                     tAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGAAACCATGCCGTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTGAGgtttaagt                                    <  1:562072/141‑1 (MQ=14)
                                                                                                      aaaTACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATCTCGATGTACAATGACAGTCAGgtttaagtt                                   >  2:490899/1‑141 (MQ=255)
                                                                                                      aaaTACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGCTCTTGATAACTGAAGACCCTCGGATATTTCGATGTACAATGGCAGGCAGgtttaagtt                                   >  2:234963/1‑141 (MQ=14)
                                                                                                       aaTACACTCAATACCTATTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGgtttaagttt                                  >  1:459616/1‑141 (MQ=255)
                                                                                                        aTACATTCAATATCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACTGTGACCTTGCGCATTGAGGTTATTCATATATTTCTATGTACAATGACAGTCAGgtttaagttta                                 <  2:744821/141‑1 (MQ=14)
                                                                                                        aTACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGgtttaagttta                                 >  2:681575/1‑141 (MQ=255)
                                                                                                         tacacacAATACCTTTTAAGGTATTCTTTGCTGGATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATTAGGTTATTCAGATATGTCGGTGTACGTTGGCAGTCAGgtttaggtttaa                                <  2:103926/135‑1 (MQ=14)
                                                                                                         tACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGgtttaagtttaa                                >  1:707180/1‑141 (MQ=255)
                                                                                                         tACACTCAATACCTTTTAAAGTAGTCTTTGCTGTATTGATACTTTGATACGTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCCATGTACAATGGCAGTCAGgtttaagtttaa                                >  2:569235/1‑141 (MQ=14)
                                                                                                          aCATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTaaa                               >  2:13421/1‑141 (MQ=255)
                                                                                                           cactCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCATTGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTaaaa                              >  2:424562/4‑141 (MQ=255)
                                                                                                           cATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTaaaa                              >  1:735132/1‑141 (MQ=255)
                                                                                                             ctCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTaaaacc                            >  1:251407/2‑139 (MQ=255)
                                                                                                              ttaaTTCCTTTTTAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCt                           <  2:289711/139‑1 (MQ=255)
                                                                                                              tCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCt                           <  1:329756/141‑1 (MQ=255)
                                                                                                                  tACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTaa                       <  1:113780/141‑1 (MQ=255)
                                                                                                                   aCCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAAt                      <  2:735140/141‑1 (MQ=255)
                                                                                                                   aCCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAAt                      >  1:623361/1‑141 (MQ=255)
                                                                                                                   aCCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGATTTact                      >  2:773278/1‑139 (MQ=255)
                                                                                                                   aCCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTGAAt                      >  2:520271/1‑141 (MQ=255)
                                                                                                                   aCCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAACTTTAAt                      >  2:196072/1‑141 (MQ=255)
                                                                                                                    ccTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAAtt                     >  2:34520/1‑141 (MQ=255)
                                                                                                                    ccTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAACTTTAAAAGATTTAAtt                     >  2:118841/1‑141 (MQ=255)
                                                                                                                     cTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTa                    <  1:369547/141‑1 (MQ=255)
                                                                                                                       tttAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGAAGTAGGGTTAAAGGTTAAAAGCTTTAATTACt                  >  2:157153/1‑141 (MQ=14)
                                                                                                                         tAACGTATTATATGATGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGTACTTGCTAAATGAGGTTATTCCGATATTTCGATGTACAATGGCAGTCAGGTTTAAATTTAAAAGCTTTAAtttgttt                >  2:61084/1‑136 (MQ=14)
                                                                                                                         tAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTACGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACttt                >  1:441157/1‑141 (MQ=255)
                                                                                                                           aaGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTaa              >  2:601/1‑140 (MQ=255)
                                                                                                                            agcaTTCTATGCTGTATTAAGACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTaac             <  1:208000/138‑3 (MQ=14)
                                                                                                                            aGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTaac             <  1:188120/141‑3 (MQ=255)
                                                                                                                               aTTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCAt          >  1:617176/1‑141 (MQ=255)
                                                                                                                               aTTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCAt          >  1:151281/1‑141 (MQ=255)
                                                                                                                                   tttGCTGTATTGATACTTTGAGACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCCTTAATTACTTTAACCATTGCt      >  2:70107/1‑141 (MQ=255)
                                                                                                                                    ttGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTa     <  2:451101/141‑1 (MQ=255)
                                                                                                                                      gCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGCCGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTAcc   >  2:411865/1‑141 (MQ=255)
                                                                                                                                        |                                                                                                                                            
TCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAGCCATTGCTACCT  >  CP000730/35861‑36136

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: