Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 36,087 A→G 59.8% C155C (TGT→TGC USA300HOU_0026 ← IS431mec transposase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP00073036,0870AG59.8% 467.3 / inf 786C155C (TGT→TGCUSA300HOU_0026IS431mec transposase
Reads supporting (aligned to +/- strand):  ref base A (179/134);  new base G (258/207);  total (442/344)
Fisher's exact test for biased strand distribution p-value = 6.59e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.55e-01

Only 100 of 787 total aligned reads displayed.

TTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAGCCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTAT  >  CP000730/35947‑36226
                                                                                                                                             |                                                                                                                                           
tttttATATAGAGCGTAAATGCATTCAACACCTTTTAAAGTATTCTTTGCCGTAGTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGa                                                                                                                                             >  1:501487/1‑141 (MQ=14)
   ttATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAg                                                                                                                                          >  1:615969/1‑141 (MQ=39)
   ttATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAg                                                                                                                                          >  2:182770/1‑141 (MQ=255)
     atataGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCCGTc                                                                                                                                        >  1:464011/1‑141 (MQ=255)
       ataGAGCGTAAATACATTTACTCCCTTTTAAAGTATTCTTTGCCGTTTTGCTGCTTTGATCTCTTCTCTTTCGTACTTTAATATGACGATGATCTGGCACAATTAGGTTATTCAGATATTGCGATGTACAATGACAGTCAg                                                                                                                                      <  2:607126/141‑1 (MQ=14)
       ataGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAg                                                                                                                                      >  1:634770/1‑141 (MQ=255)
       ataGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAg                                                                                                                                      >  1:509129/1‑141 (MQ=255)
        taGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTAAACTGGCAGTCAgg                                                                                                                                     >  2:102141/1‑141 (MQ=255)
          gagCGTAGATACAGTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGgtt                                                                                                                                   >  2:669933/1‑141 (MQ=255)
               tAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGgtttaagt                                                                                                                              <  1:33439/141‑1 (MQ=255)
               tAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATCTGACACCACGTTGTCGCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGgtttaagt                                                                                                                              >  1:761018/1‑141 (MQ=11)
                 aaTACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGgtttaagttt                                                                                                                            >  2:666027/1‑141 (MQ=255)
                   tACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGgttaataattta                                                                                                                          <  2:302504/141‑10 (MQ=14)
                   tACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGgtttaagtttaa                                                                                                                          >  1:100962/1‑141 (MQ=255)
                   tACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGgtttaagtttaa                                                                                                                          >  2:445327/1‑141 (MQ=255)
                   tACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGgtttaagtttaa                                                                                                                          >  1:707180/1‑141 (MQ=255)
                    aCATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCACGTTTAAGTTTaaa                                                                                                                         >  2:361354/1‑141 (MQ=255)
                    aCATACAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGGTATGTTGACTTTCTTCCTTTAATATGACGGCTATCTTGCTCAATAAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGTTTTAAGTTTaaa                                                                                                                         >  2:210675/1‑141 (MQ=14)
                     cactCAATACCTTTTAAAGTATTCTTTGCTGTATTGATATTTTGATAACTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGACGTACAGTGGCAGTCAGGTTTAAGTTTaaaa                                                                                                                        >  2:485057/4‑141 (MQ=11)
                     cATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTaaaa                                                                                                                        >  1:735132/1‑141 (MQ=255)
                        ttaaTTCCTTTTTAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCt                                                                                                                     <  2:289711/139‑1 (MQ=255)
                          aaTACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCttt                                                                                                                   <  1:670794/141‑1 (MQ=255)
                           aTACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTa                                                                                                                  >  2:195733/1‑141 (MQ=255)
                            tACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTaa                                                                                                                 <  1:321971/141‑1 (MQ=255)
                             aCCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAAt                                                                                                                >  1:623361/1‑141 (MQ=255)
                              ccTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAACTTTAAAAGATTTAAtt                                                                                                               >  2:118841/1‑141 (MQ=255)
                               atattAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTAAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTa                                                                                                              >  2:343189/4‑141 (MQ=255)
                                ttttAAGGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTat                                                                                                             <  2:726541/141‑2 (MQ=255)
                                ttttAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTAc                                                                                                             <  1:252783/141‑1 (MQ=255)
                                ttttAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAACCTTTAATTAc                                                                                                             >  2:228458/1‑141 (MQ=255)
                                 tttAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGAAGTAGGGTTAAAGGTTAAAAGCTTTAATTACt                                                                                                            >  2:157153/1‑141 (MQ=14)
                                  ttAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTattt                                                                                                           >  1:648525/1‑138 (MQ=255)
                                   tAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTACGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACttt                                                                                                          >  1:441157/1‑141 (MQ=255)
                                   tAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTAtttt                                                                                                          <  2:133967/141‑1 (MQ=255)
                                    aaaGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCTGTCTGGTTTAAGTTTAAAAGCTTTAATTACTTTa                                                                                                         >  1:478820/1‑141 (MQ=255)
                                     aaGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCCGGTTTAAGTTTAAAAGCTTTAATTAtttttg                                                                                                        >  2:25052/1‑139 (MQ=14)
                                      agcaTTCTATGCTGTATTAAGACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTaac                                                                                                       <  1:208000/138‑3 (MQ=14)
                                        tATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCtt                                                                                                                    >  1:215734/1‑126 (MQ=255)
                                           tCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTg                                                                                                  <  1:539898/141‑1 (MQ=255)
                                             tttGCTGTATTGATACTTTGATTCCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCt                                                                                                <  1:186238/141‑1 (MQ=255)
                                             tttGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCt                                                                                                <  1:109676/141‑1 (MQ=255)
                                              ttGCCGTATTGATAATGTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTATAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCtg                                                                                               >  1:429477/1‑140 (MQ=14)
                                                gccgTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCtgcc                                                                                             <  1:748735/138‑4 (MQ=255)
                                                gCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTACTTACTTTAACCATTGCTAcc                                                                                             >  1:274601/1‑141 (MQ=255)
                                                gCTGTATTGATACCTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAGCCATTGCTAcc                                                                                             <  1:142565/141‑1 (MQ=255)
                                                 ccgTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCt                                                                                            <  2:106794/139‑1 (MQ=255)
                                                 cTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCt                                                                                            <  1:350593/141‑1 (MQ=255)
                                                     aTTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGt                                                                                        >  1:717529/1‑141 (MQ=255)
                                                      ttGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAAGTACTTTAGCCATTGCTACCTTCGtt                                                                                       <  2:426570/141‑1 (MQ=255)
                                                          tACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAg                                                                                   >  1:679993/1‑141 (MQ=255)
                                                          tACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGGTTGAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAg                                                                                   >  1:401750/1‑141 (MQ=255)
                                                           aCTTTGATACCTTGTCTTTCTTACTTTAATAGGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAGCCATTGCTACCTTCGTTGAAgg                                                                                  >  2:548766/1‑141 (MQ=255)
                                                              ttGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCGGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGc                                                                               >  2:414692/1‑141 (MQ=255)
                                                               tGATATCGTGTTTTTCTTACTTTAATATGACGGTGATCTTTCTCAATGAGGTTATTCAGATATTTCGATGTACAATGTCAGCCAGGTTTAAGTTTAAAATCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGcc                                                                              <  2:14083/141‑1 (MQ=11)
                                                                 atatcTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCTGATTTTTTGTTGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTTGCCATTGCTGCCTTGGTTGAAGGTACCTg                                                                            >  1:259184/5‑141 (MQ=11)
                                                                 aTACCTTGTGTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTg                                                                            >  2:496737/1‑141 (MQ=255)
                                                                  tatcTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATGTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGa                                                                           >  1:524913/4‑141 (MQ=255)
                                                                         tctttcttACTTTAATATGCCGGTGATCTTGCTCAGTGAGGTTATTCAGATATTTCGATGTACACTGGCAGGCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTCCCTTCGTTGAATGGGCCCGagtgtcat                                                                    >  2:405211/1‑134 (MQ=11)
                                                                              ctttctttAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTAcc                                                               <  2:630990/141‑1 (MQ=255)
                                                                              cttACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTAcc                                                               >  2:576794/1‑141 (MQ=255)
                                                                              cttACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAGCCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTAcc                                                               >  1:129267/1‑141 (MQ=38)
                                                                                tACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCtt                                                             <  2:173269/141‑1 (MQ=255)
                                                                                tACTTTAATATGACGGTGATCTTGCTCAATGAGGGTATTCAGATATTTCGATGTACAATGCCAGTGAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTGACTACCtt                                                             >  2:752007/1‑141 (MQ=11)
                                                                                 tcTTTAATAGGACGGTTATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTCCGTTGAAGGTGCCTGATCTGTAATTACCttt                                                            <  1:600733/140‑1 (MQ=11)
                                                                                 aCTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTGACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAAGTAACttt                                                            >  2:741984/1‑141 (MQ=11)
                                                                                 aCTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCttt                                                            >  2:460768/1‑141 (MQ=255)
                                                                                     tAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAg                                                        >  1:660032/1‑141 (MQ=255)
                                                                                        tatGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCTGGTTTTAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTTATCTGTAATTACCTTTTTAGGtt                                                     >  1:153727/1‑141 (MQ=11)
                                                                                           gaggggtaTCTTGCTCAATGTGGTTGTTTAGATATTTCGAGGTTGATGGGCAGTCATGTTTCTGTGTAATAGGTTTTATTACTTTAACAATTGGTACCTTTGTTGTAGGTGGCTGTGCTGTAATTACCTTTTGAGGTTTAc                                                  <  2:69236/134‑1 (MQ=2)
                                                                                            ccggtcttCTTGCGGAATGCGATTATTTTAATAGTTCTTTGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTAcc                                                 <  1:173910/134‑1 (MQ=11)
                                                                                            aCGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTAcc                                                 >  1:767027/1‑141 (MQ=25)
                                                                                             cGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTGAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGGGCCTGATCTGTATTTACCTTTTGAGGTTTACCa                                                >  2:241643/1‑141 (MQ=11)
                                                                                                 gATCTTGCTCAATGGGGTCAATCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGCAGGTGCCTGATCTGTAACTACCTTTTGAGGTTTAcctgatt                                            <  2:474889/141‑6 (MQ=11)
                                                                                                   tCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAGCCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCACATTGt                                          >  2:64952/1‑141 (MQ=17)
                                                                                                      tGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTa                                       >  2:524454/1‑141 (MQ=255)
                                                                                                          ttaaTGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATg                                    <  1:234963/139‑1 (MQ=12)
                                                                                                           cAATGAGGTTATTCCGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTaaata                                   <  2:710864/141‑4 (MQ=11)
                                                                                                              tGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGAc                                <  1:286966/141‑1 (MQ=17)
                                                                                                               gAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCGGCAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACg                               <  1:411865/141‑1 (MQ=255)
                                                                                                               gAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAGCCATTTCTACCTTCATTGAAGGTGCCTGATCTGTAATTACCTTTTTAGGTTTACCAAATGGTTTAATGAGACg                               >  1:378430/1‑141 (MQ=255)
                                                                                                                  gTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGttt                            <  2:562553/141‑1 (MQ=17)
                                                                                                                   ttATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTCATGAGACGTTTg                           <  2:569050/141‑1 (MQ=12)
                                                                                                                     aTTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGAt                         >  1:297455/1‑141 (MQ=12)
                                                                                                                      ttCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATa                        <  2:82691/141‑1 (MQ=255)
                                                                                                                       tCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATaa                       >  2:615814/1‑141 (MQ=17)
                                                                                                                        cAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATaaa                      >  2:328882/1‑141 (MQ=12)
                                                                                                                        cAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATaaa                      >  1:365113/1‑141 (MQ=17)
                                                                                                                         aGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAAc                     <  2:764268/141‑1 (MQ=17)
                                                                                                                         aGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAAc                     <  2:588595/141‑1 (MQ=17)
                                                                                                                         aGATATTTCCATGGACAATGGCAGTTAGATTATAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTCTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAAc                     <  1:692294/141‑1 (MQ=11)
                                                                                                                          aatatTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAGCCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACg                    <  2:39537/140‑1 (MQ=17)
                                                                                                                            tatTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAGCCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCa                  <  1:548766/141‑1 (MQ=32)
                                                                                                                               ttCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCctat               >  1:89789/1‑141 (MQ=12)
                                                                                                                               tgcGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTAACTTTAGCGGTGTACCAGATTGTTTAATGAGACGTTTGATAAACGCatat               >  2:349618/3‑141 (MQ=11)
                                                                                                                                 cGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGc             <  1:582766/141‑1 (MQ=11)
                                                                                                                                  gATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCCTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCt            >  2:272011/1‑141 (MQ=12)
                                                                                                                                    tGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGGGGTTTATCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGa          <  2:377238/141‑1 (MQ=255)
                                                                                                                                       aCAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTCCCCTTTGGGGTGTACTCTATATTGTAATGAGACGTTTGATAACCGCACTTGCTGAATg       <  1:566500/141‑1 (MQ=11)
                                                                                                                                           tGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTa   <  2:683713/141‑1 (MQ=255)
                                                                                                                                            gACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTAt  >  1:391667/1‑141 (MQ=255)
                                                                                                                                             |                                                                                                                                           
TTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAGCCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTAT  >  CP000730/35947‑36226

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: