Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 36,133 A→G 31.6% V140A (GTA→GCA)  USA300HOU_0026 ← IS431mec transposase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP00073036,1330AG31.6% 523.2 / inf 582V140A (GTA→GCA) USA300HOU_0026IS431mec transposase
Reads supporting (aligned to +/- strand):  ref base A (202/188);  new base G (99/83);  total (307/275)
Fisher's exact test for biased strand distribution p-value = 5.90e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.00e-01

Only 100 of 593 total aligned reads displayed.

TTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAGCCA‑TTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCA  >  CP000730/35993‑36273
                                                                                                                                             |                                                                                                                                            
ttGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGTTGATCTTGCTCAATGTGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTTCTTTACCCA‑TTGCTa                                                                                                                                              >  1:207573/1‑141 (MQ=14)
 tGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCA‑TTGCTAc                                                                                                                                             <  2:223939/141‑1 (MQ=255)
  gCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTACTTACTTTAACCA‑TTGCTAcc                                                                                                                                            >  1:274601/1‑141 (MQ=255)
  gCTGTATTGATACCTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAGCCA‑TTGCTAcc                                                                                                                                            <  1:142565/141‑1 (MQ=255)
   cTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCA‑TTGCTTCCt                                                                                                                                           >  2:117713/1‑141 (MQ=255)
      tATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCA‑TTGCTGCCTTca                                                                                                                                        >  2:575495/1‑140 (MQ=255)
      tATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGAGTAAAAGCTTTAATTACTTTAACCA‑TTGCTACCTCCg                                                                                                                                        >  2:421839/1‑141 (MQ=14)
          gATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCCGGTTTAAGTTTCAAAGCTTTAATTACTTTAACCA‑TTGCTACCTTCGTTGa                                                                                                                                    >  2:206303/1‑141 (MQ=255)
          gATACTTTGATAACTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCA‑TTGCTACCTTCGTTGa                                                                                                                                    <  1:70863/141‑1 (MQ=255)
            tACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCA‑TTGCTACCTTCGTTGAAg                                                                                                                                  <  1:441468/141‑1 (MQ=255)
            tACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGGTTGAAAGCTTTAATTACTTTAACCA‑TTGCTACCTTCGTTGAAg                                                                                                                                  >  1:401750/1‑141 (MQ=255)
            tACTTTCATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGATTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCA‑TTGCTGCCTTCGTTGAAg                                                                                                                                  >  2:487770/1‑141 (MQ=14)
             aCTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAGCCA‑TTGCTACCTTCGTTGAAgg                                                                                                                                 >  1:318394/1‑141 (MQ=40)
              cTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGTTCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTATTTATTTTTGCCA‑TTGCTGCCTTCGTGTAAGGt                                                                                                                                >  1:680467/1‑141 (MQ=11)
              cTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTGAATGGGGTTATTCAGATCTTTCGATGTACCATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCA‑TTGCTGCCTTCGTTGAAGGt                                                                                                                                <  2:1795/141‑1 (MQ=11)
               tttGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCA‑TTGCTACCTTCGTTGAAGGTg                                                                                                                               <  1:621476/141‑1 (MQ=255)
                 tGATATCGTGTTTTTCTTACTTTAATATGACGGTGATCTTTCTCAATGAGGTTATTCAGATATTTCGATGTACAATGTCAGCCAGGTTTAAGTTTAAAATCTTTAATTACTTTAACCA‑TTGCTACCTTCGTTGAAGGTGcc                                                                                                                             <  2:14083/141‑1 (MQ=11)
                  tattccTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCA‑TTGCTACCTTCGTTGAAGGTGCCt                                                                                                                            <  2:344233/137‑1 (MQ=255)
                   atatcTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCA‑TTGCTGCCTTCGTTGAAGGTGCCTg                                                                                                                           >  1:736988/5‑141 (MQ=255)
                    tatcTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATGTTAGCCA‑TTGCTGCCTTCGTTGAAGGTGCCTGa                                                                                                                          >  1:524913/4‑141 (MQ=255)
                     aCCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCA‑TTGCTACCTTCGTTGAAGGTGCCTGAt                                                                                                                         >  2:160256/1‑141 (MQ=255)
                      tcTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCCGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCA‑TTGCTGCCTTCGTTGAAGGTGCCTGATc                                                                                                                        >  1:103423/2‑141 (MQ=255)
                       cTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAAGCA‑TTGCTAGCTTCGTTGAATGTGCGTGATCt                                                                                                                       >  2:52978/1‑141 (MQ=11)
                         tGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCA‑TTGCTACCTTCGTTGAAGGTGCCTGATCTGt                                                                                                                     <  2:55315/141‑1 (MQ=255)
                           tctttcttACTTTAATATGCCGGTGATCTTGCTCAGTGAGGTTATTCAGATATTTCGATGTACACTGGCAGGCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCA‑TTGCTCCCTTCGTTGAATGGGCCCGagtgtcat                                                                                                                   >  2:405211/1‑134 (MQ=11)
                             tttcttACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCA‑TTGCTACCTTCGTTGAAGGTGCCTGATCTGTAAtt                                                                                                                 <  1:753632/141‑1 (MQ=255)
                              ttcttACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCG‑TTGCTGCCTTCGTTGAAGGGGTCTGAtatctcactc                                                                                                                >  1:93267/1‑132 (MQ=14)
                               tcttACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTATTTATTTTAGCCA‑TTGCTGCCTTGGTTGATGGTGCCTTATCTGTGATTAc                                                                                                               >  1:572932/1‑141 (MQ=11)
                                ctttctttAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCA‑TTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTAcc                                                                                                              <  2:630990/141‑1 (MQ=255)
                                 ttACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCA‑TTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCt                                                                                                             <  2:308794/141‑1 (MQ=255)
                                  tACTTTAATATGACGGTGATCTTGCTCAATGAGGGTATTCAGATATTTCGATGTACAATGCCAGTGAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCA‑TTGCTGCCTTCGTTGAAGGTGCCTGATCTGTGACTACCtt                                                                                                            >  2:752007/1‑141 (MQ=11)
                                  tACTTTAATATGACGGTGAACTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCA‑TTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCtt                                                                                                            >  2:388706/1‑141 (MQ=255)
                                    ttttAATAGGATGGGTATCTTGCTCAATGAGGTTATTCAGATATTTCGGTGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAGCCA‑TTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCtttt                                                                                                          <  2:287414/140‑1 (MQ=11)
                                      ttAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATAACTTTAACCA‑TTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGa                                                                                                        <  1:514935/141‑1 (MQ=255)
                                        aaTATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCCTTAATTATTTTAGCCA‑TTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAgg                                                                                                      <  1:297210/141‑1 (MQ=255)
                                          tatGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCTGGTTTTAGTTTAAAAGCTTTAATTACTTTAACCA‑TTGCTACCTTCGTTGAAGGTGCCTTATCTGTAATTACCTTTTTAGGtt                                                                                                    >  1:153727/1‑141 (MQ=11)
                                           atGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCA‑TTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGctt                                                                                                   >  1:535651/1‑141 (MQ=255)
                                             gACCGTCATCTTGCTCAATGAGGTTAGTCAGATAGTTCGAGGTAGACCGGCAGTCAGGTTTAAGTGTAAAAGCTTTAATTACTTTAACCA‑TTGCTACCTTCGGTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTAc                                                                                                 <  1:484996/141‑1 (MQ=11)
                                              aCGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCA‑TTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTAcc                                                                                                <  2:607444/141‑1 (MQ=21)
                                              aCGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCA‑TTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTAcc                                                                                                >  1:506361/1‑141 (MQ=19)
                                              aCGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCA‑TTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTAcc                                                                                                >  2:722297/1‑141 (MQ=25)
                                              aCGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCA‑TTGGTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTAcc                                                                                                <  1:729934/141‑1 (MQ=11)
                                               cGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTGAAAGCTTTAATTACTTTAACCA‑TTGCTACCTTCGTTGAAGGGGCCTGATCTGTATTTACCTTTTGAGGTTTACCa                                                                                               >  2:241643/1‑141 (MQ=11)
                                                   gATCTTGCTCAATGGGGTCAATCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCA‑TTGCTGCCTTCGTTGCAGGTGCCTGATCTGTAACTACCTTTTGAGGTTTAcctgatt                                                                                           <  2:474889/141‑6 (MQ=11)
                                                    aTCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTTATTATTTTAGCCA‑TTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTg                                                                                          >  1:17398/1‑141 (MQ=11)
                                                    aTCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTA‑ATCAGCCACACTCGACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTg                                                                                          >  1:761160/1‑141 (MQ=11)
                                                     tCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCA‑TTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGt                                                                                         >  1:131503/1‑141 (MQ=12)
                                                      cTTGCTCAATGAGGTTATTCAGGTAGGTCGATGTACAATGACAGTCAGGTTTAAGTTTAGAAGCTTTAATTATTTTAGCCA‑TTGGTCCCTTGGTTCCGGTGGTCCGGTCTGTACCTACCTTTCGAGGCTTACCAACTTGtt                                                                                        >  2:741222/1‑141 (MQ=2)
                                                      cTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCA‑TTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGtt                                                                                        >  1:392852/1‑141 (MQ=255)
                                                      cTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCA‑TTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGtt                                                                                        >  1:634321/1‑141 (MQ=11)
                                                       ttGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCA‑TTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGttt                                                                                       >  2:359990/1‑141 (MQ=12)
                                                          cTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCA‑TTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAAt                                                                                    <  1:416613/141‑1 (MQ=12)
                                                            cAATGCGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCA‑TTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATga                                                                                  <  1:586895/141‑1 (MQ=12)
                                                                 aGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCA‑TTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGt                                                                             >  2:188486/1‑141 (MQ=17)
                                                                  ggTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCA‑TTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGtt                                                                            >  2:667787/1‑141 (MQ=255)
                                                                   gTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCA‑TTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGttt                                                                           <  2:562553/141‑1 (MQ=17)
                                                                   gTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCCTTAATTACTTTAACCA‑TTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGttt                                                                           <  1:70107/141‑1 (MQ=12)
                                                                    ttATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCA‑TTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTCATGAGACGTTTg                                                                          <  2:569050/141‑1 (MQ=12)
                                                                    ttATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCA‑TTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTg                                                                          >  2:295833/1‑141 (MQ=11)
                                                                     tATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCA‑TTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTCATGAGACGTTTGa                                                                         <  2:453346/141‑1 (MQ=12)
                                                                       ttCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCA‑TTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATa                                                                       <  2:82691/141‑1 (MQ=255)
                                                                         cAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCA‑TTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTTAGGTTTACCAAATTGTTTAATGAGACGTTTGGTaaa                                                                     >  2:104717/1‑141 (MQ=255)
                                                                         cAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCA‑TTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATaaa                                                                     >  2:328882/1‑141 (MQ=12)
                                                                          aGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCA‑TTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAAc                                                                    <  2:588595/141‑1 (MQ=17)
                                                                           gATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCA‑TTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACg                                                                   >  1:281310/1‑141 (MQ=17)
                                                                           gATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCA‑TTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACg                                                                   >  2:368174/1‑141 (MQ=11)
                                                                             tatTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCA‑TTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCa                                                                 <  1:770659/141‑1 (MQ=11)
                                                                             tatTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAGCCA‑TTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCa                                                                 <  1:548766/141‑1 (MQ=32)
                                                                                  cGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCA‑TTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGc                                                            <  1:238339/141‑1 (MQ=17)
                                                                                   gATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCA‑TTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCt                                                           >  1:219974/1‑141 (MQ=17)
                                                                                   gATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCA‑TTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCt                                                           >  2:620335/1‑141 (MQ=12)
                                                                                   gATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCA‑TTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCt                                                           >  1:659373/1‑141 (MQ=17)
                                                                                   gATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCA‑TTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCCTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCt                                                           >  2:272011/1‑141 (MQ=12)
                                                                                    aTGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAGCCA‑TTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATctgctg                                                          >  1:383732/1‑141 (MQ=17)
                                                                                        aaaaTGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCA‑TTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATg                                                      <  2:251407/139‑1 (MQ=9)
                                                                                        aCAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCA‑TTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGGATg                                                      >  1:473928/1‑141 (MQ=12)
                                                                                          aaTGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCA‑TTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTCTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGAt                                                    <  1:603016/141‑1 (MQ=9)
                                                                                           aTGACAGTCAGGTTTGCGTTTAAAAGCTTTAATTATTTTAGCCA‑TTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGAtt                                                   <  2:699815/141‑1 (MQ=11)
                                                                                            tGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCA‑TTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTa                                                  <  2:683713/141‑1 (MQ=255)
                                                                                             gACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCA‑TTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTAt                                                 >  1:688789/1‑141 (MQ=2)
                                                                                             gACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCA‑TTGCTGCCTGCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACTCATATGCTGAATGATTAt                                                 >  2:413870/1‑141 (MQ=11)
                                                                                                                            tattttAGCCA‑TTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAatgt                  <  1:667415/138‑1 (MQ=0)
                                                                                                                               tttAACCA‑TTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGAGAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAatgtatg               >  2:662714/1‑141 (MQ=11)
                                                                                                                               tttAACCA‑TTGCTACCTTCGTTGAAGGTGCC‑GATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGAAAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCCACTATCCAatgtatgt              >  1:368095/1‑141 (MQ=11)
                                                                                                                               tttAACCA‑GTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAatgtatg               <  2:763168/141‑1 (MQ=11)
                                                                                                                                ttAGCCA‑TTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAatgtatgt              <  2:64331/141‑1 (MQ=9)
                                                                                                                                 ttgacA‑TGGCTGCCTTCCGTGAAGGTGCCTAAACTGTAACTACCTTTTGAGGCTTAGCAAATTGTTTAACGAGACGTTAGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTc             <  2:1307/137‑1 (MQ=11)
                                                                                                                                 taaccA‑TTGCAACCTTGTTTGAGGGTGCCTGATC‑GTCAATACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTcc            <  1:346474/138‑1 (MQ=11)
                                                                                                                                 tAGCCA‑GTGCTGCCTTCGTTGTACGCGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTc             <  1:328636/141‑1 (MQ=11)
                                                                                                                                  aGCCA‑TTGCTGCCTTCGTTTAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTcc            <  2:462392/141‑1 (MQ=255)
                                                                                                                                    ccA‑TTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAAGTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCct          <  2:509129/141‑1 (MQ=17)
                                                                                                                                      a‑TTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTTTCTCGTTGCTTACGCAACCAAATATCTAATGTATGTTCctct        >  1:262463/1‑141 (MQ=17)
                                                                                                                                        ttGCTTCCTTCGTTGAAGGTGTCTGATCTGTATTTACCTTTTGAGGTTTACCAAATTGTTTAATGGGTCGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTg       <  1:111410/141‑1 (MQ=11)
                                                                                                                                        ttGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATATATGTCCctctt       >  1:645667/1‑140 (MQ=17)
                                                                                                                                         tgctgcCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTGAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTCACGCAACCAAATATCTAATGTATGTCCCTCTgt      >  2:431904/1‑140 (MQ=11)
                                                                                                                                          gCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTgta     <  1:723410/141‑3 (MQ=17)
                                                                                                                                          gCTAACTTTGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAGACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTgta     <  1:355909/141‑3 (MQ=12)
                                                                                                                                           cTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTAt    >  1:672724/1‑141 (MQ=17)
                                                                                                                                            tACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATc   >  1:255991/1‑141 (MQ=32)
                                                                                                                                             aCCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCa  <  2:398881/141‑1 (MQ=32)
                                                                                                                                             |                                                                                                                                            
TTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAGCCA‑TTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCA  >  CP000730/35993‑36273

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑