Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 36,174 T→C 34.0% K126K (AAA→AAG USA300HOU_0026 ← IS431mec transposase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP00073036,1740TC34.0% 418.9 / inf 595K126K (AAA→AAGUSA300HOU_0026IS431mec transposase
Reads supporting (aligned to +/- strand):  ref base T (190/199);  new base C (100/104);  total (292/303)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.58e-01

Only 100 of 607 total aligned reads displayed.

ATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAGCCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGAT  >  CP000730/36034‑36314
                                                                                                                                            |                                                                                                                                             
    gACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGAGAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTAc                                                                                                                                           >  1:512959/1‑141 (MQ=11)
    gACGGTCATCCTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCGGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTAc                                                                                                                                           <  1:753633/141‑1 (MQ=11)
    gACGGGGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCACGTTTAAGTTTTACAGCTTTAATTACTGTTTCCATTTCTACCTTCGTTGAAGGTGCCTTTTCTGTAATTACCTTTTGAGGTTTAc                                                                                                                                           <  1:145932/141‑1 (MQ=11)
     aCGGTGATCTTACTCAATGAGGTTATTCAGATATATCGATGTACAATGACAGGCAGGATTAAGTTTAACAGTTTTAATTATTTTAGCCATAGCTGACTTCGTTGCAGGTGACTGAACTGTAACTACCTATTGAGGCTGAcc                                                                                                                                          >  2:210443/1‑141 (MQ=11)
        gTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCaaa                                                                                                                                       <  2:100378/141‑1 (MQ=17)
         tGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAGCCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAAt                                                                                                                                      >  1:297758/1‑141 (MQ=32)
          gATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAAtt                                                                                                                                     <  2:282232/141‑1 (MQ=255)
           aTCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTg                                                                                                                                    <  2:678660/141‑1 (MQ=17)
           aTCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCCTACCAAATTg                                                                                                                                    >  1:713851/1‑141 (MQ=11)
            tCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGt                                                                                                                                   >  2:369861/1‑141 (MQ=12)
            tCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGt                                                                                                                                   >  2:667415/1‑141 (MQ=255)
             cTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGtt                                                                                                                                  >  1:392852/1‑141 (MQ=255)
             cTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGtt                                                                                                                                  >  1:634321/1‑141 (MQ=11)
                gCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAGCCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTaa                                                                                                                               >  2:298137/1‑141 (MQ=32)
                 gtCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAAt                                                                                                                              <  1:549/140‑1 (MQ=11)
                 cTCAATGAGGTTATTCAGATATTTAGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGATGGTGCCTTATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAAt                                                                                                                              <  2:484216/141‑1 (MQ=11)
                  ttaaTGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATg                                                                                                                             <  1:234963/139‑1 (MQ=12)
                   cAATGAGGTTATTCCGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTaaata                                                                                                                            <  2:710864/141‑4 (MQ=11)
                   cAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATga                                                                                                                            <  1:134906/141‑1 (MQ=255)
                        gggTTATTTAGATTTTTTGATGTACATTGGCTGGCAGTTTTATGTTTAAAAGGTTTAATTGCTTTATCCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGt                                                                                                                       <  2:408221/140‑1 (MQ=11)
                         ggTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGtt                                                                                                                      <  1:661625/141‑1 (MQ=17)
                         ggTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGtt                                                                                                                      >  2:244132/1‑141 (MQ=11)
                         ggTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGtt                                                                                                                      >  1:274442/1‑141 (MQ=11)
                          gTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCCTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGttt                                                                                                                     <  1:70107/141‑1 (MQ=12)
                          gTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGttt                                                                                                                     >  1:484295/1‑141 (MQ=255)
                           ttATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTg                                                                                                                    >  2:533378/1‑141 (MQ=11)
                            tATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGa                                                                                                                   <  1:345814/141‑1 (MQ=11)
                             aTTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTAGCAAATTGTTTAATGAGACGTTTGAt                                                                                                                  >  1:617508/1‑141 (MQ=12)
                             aTTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGGTTTAATTGTTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGAt                                                                                                                  >  1:602676/1‑141 (MQ=11)
                                cAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATaaa                                                                                                              >  2:134363/1‑141 (MQ=12)
                                cAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATaaa                                                                                                              >  2:328882/1‑141 (MQ=12)
                                 aGATATTTCCATGGACAATGGCAGTTAGATTATAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTCTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAAc                                                                                                             <  1:692294/141‑1 (MQ=11)
                                  gttatTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACg                                                                                                            <  2:569599/139‑1 (MQ=12)
                                    tatTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCa                                                                                                          <  1:770659/141‑1 (MQ=11)
                                       ttCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCctat                                                                                                       >  1:89789/1‑141 (MQ=12)
                                         cGATGTACAATGGCAGTCAGGATTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGc                                                                                                     >  1:401648/1‑141 (MQ=255)
                                          gATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCt                                                                                                    >  2:620335/1‑141 (MQ=12)
                                          gATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCCTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCt                                                                                                    >  2:272011/1‑141 (MQ=12)
                                             gTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGaa                                                                                                 <  1:415137/141‑1 (MQ=17)
                                               aCAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTCCCCTTTGGGGTGTACTCTATATTGTAATGAGACGTTTGATAACCGCACTTGCTGAATg                                                                                               <  1:566500/141‑1 (MQ=11)
                                               aCAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATg                                                                                               <  1:249819/141‑1 (MQ=17)
                                                cAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAGCCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGa                                                                                              >  2:294514/1‑141 (MQ=32)
                                                 aaTGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGAt                                                                                             <  1:104700/141‑1 (MQ=14)
                                                  aTGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTGGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGCCTTACCAACTTGTTTAATGAGACGTTTGATAAACGCGTATGCTGAATGAtt                                                                                            >  2:451907/1‑141 (MQ=11)
                                                    gACAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAGCCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTAt                                                                                          <  1:20975/141‑1 (MQ=11)
                                                                                      tttAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAatgtatg                                                        >  1:18233/1‑141 (MQ=2)
                                                                                        tAGCCCTTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACAACATTTTGAGGCTTACCAAATTGTTTAATGAGACGTCTGATAAACGAATATGCTGAATGAGTATCTCGTTGCTTACGCACCCAAATGTCTAATGTATGTc                                                      <  2:642025/141‑1 (MQ=11)
                                                                                         aaccATTGCGACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAAGTTTACCAAATTGTTTAATGAGACGTTTGATAGACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTcc                                                     <  1:473820/139‑1 (MQ=255)
                                                                                         aGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTcc                                                     <  2:42139/141‑1 (MQ=12)
                                                                                              ttGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATATATGTCCctctt                                                >  1:645667/1‑140 (MQ=17)
                                                                                                   gccTTCTTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCa                                           <  2:655159/140‑1 (MQ=11)
                                                                                                   gccTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCa                                           <  2:634321/140‑1 (MQ=255)
                                                                                                   aCCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATCATCACTGAAACGCAATGATTATCTCGTTGCTTACGCAACCAAATATATAATGTATGTCCCTCTGCATCa                                           >  1:761582/1‑141 (MQ=11)
                                                                                                   aCCTTCGTGGAAGGTGCCTGTTGTGTAATGACCTTTTGAGGTTTACCAAATGGTTTAAGGAGCCGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCa                                           <  1:682323/141‑1 (MQ=11)
                                                                                                    cctgctctaaatgTGCCTGTTCTTAAATTACCTTTTGAGTTTTACCAAATTGTTTAATGAGACGTTTGATAACCGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCaa                                          <  1:193515/129‑1 (MQ=11)
                                                                                                    ccTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCaa                                          <  2:461510/141‑1 (MQ=32)
                                                                                                     cTTCGTTGAAGGTGCCTGTTCTGTAACTACCTTTTGAGGCTTCCCAGATTGTCTATTGAGTCGTTTGGTGAACGCGTATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAAt                                         <  1:221671/141‑1 (MQ=11)
                                                                                                     cTTCGTTGAAGGTGCCTGATCTGTAACTCCCTTTTGAGGCGTACTAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCATCTGTATCAAt                                         >  1:730934/1‑141 (MQ=11)
                                                                                                      ttCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGTTAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAATTATCTAATGTATGTCCCTCTGCATCAATg                                        >  1:340933/1‑141 (MQ=17)
                                                                                                      ttCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATg                                        <  2:223041/141‑1 (MQ=255)
                                                                                                         gTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCACTGGCa                                     >  2:400619/1‑141 (MQ=17)
                                                                                                          tttaaGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTTTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCAc                                    <  1:157212/138‑1 (MQ=11)
                                                                                                           tGAAGGTGTCGGATCTGTAATTTCCTTTTGAGGTTTACCGAATTGTTTCATGAGACGTTTGCTAAGCGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACg                                   <  1:113942/141‑1 (MQ=11)
                                                                                                           tGAAGGGGCCTGACCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACg                                   <  2:148591/141‑1 (MQ=11)
                                                                                                            gAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGa                                  <  2:353193/141‑1 (MQ=32)
                                                                                                             aaGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCACCCACATATCTCATGTATGTACCTCTGCAACAATAGCagcac                                 >  1:540632/1‑137 (MQ=11)
                                                                                                               ggTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGatat                               >  2:561109/1‑141 (MQ=32)
                                                                                                                gggCCTGAGCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGAtata                              <  1:361354/139‑1 (MQ=11)
                                                                                                                gTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATCGCACGAtata                              <  2:168833/141‑1 (MQ=17)
                                                                                                                  gCCTGATCTGTAATAACCTATTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATaaa                            >  1:425486/1‑141 (MQ=17)
                                                                                                                   ccTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCCAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAAt                           >  2:118240/1‑141 (MQ=17)
                                                                                                                   ccTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATAGCTAATGTATGTCCCTCGGTATCAATGGCACGATATaaac                           >  2:181663/1‑140 (MQ=12)
                                                                                                                   ccTGATCTGTAACTACATTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAAt                           >  1:385217/1‑141 (MQ=255)
                                                                                                                    cataTCTGTAACTATCTTTTGAGGCTTACCAAATTTTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTTCGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATa                          <  2:473544/138‑1 (MQ=11)
                                                                                                                     tGATCTGTAATTACCTTTTGAGGTTTACCACATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAg                         >  1:497936/1‑141 (MQ=17)
                                                                                                                     tGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCACATATACAATGTATGTCCCTCTGTATCAATGGCACGAAATAAATAg                         >  2:393317/1‑141 (MQ=11)
                                                                                                                     tGATCTGTAACTACATTTTGAGGCTTACCAAATTGTTTAATGAGACGTATGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCACATAACTAATGTATGTACCACTGTATCAATGGAACGATGTaaaatg                         >  2:589721/1‑138 (MQ=11)
                                                                                                                      gATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGACACGATATAAATaac                        >  1:317097/1‑139 (MQ=12)
                                                                                                                      gATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGc                        <  1:675970/141‑1 (MQ=12)
                                                                                                                       aTCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGCCGTTTGCTCTTACGCTCAACTTAATCATTCTCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCt                       <  2:545221/141‑1 (MQ=11)
                                                                                                                       aTCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCt                       <  2:758951/141‑1 (MQ=17)
                                                                                                                        tCTGTAATTAGCTTTTTAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTc                      <  2:68863/141‑1 (MQ=17)
                                                                                                                         cTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTCACGCAACCAAAAACCTAACGTATGTCCCTCTGTATCAATGGCACGACATAAATAGCAcc                     >  2:80251/1‑141 (MQ=11)
                                                                                                                           gTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCAt                   <  2:344243/141‑1 (MQ=17)
                                                                                                                            tAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCAtt                  <  1:287666/141‑1 (MQ=17)
                                                                                                                             aactACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCAttt                 <  2:746277/138‑1 (MQ=12)
                                                                                                                              aTTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTGCCTCTGCATCAATGGCACGATATAAATCGCTCCAtttt                >  2:608841/1‑141 (MQ=17)
                                                                                                                               ttACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTc               >  1:261466/1‑141 (MQ=32)
                                                                                                                               ctACCGTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGACAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTc               <  2:501487/140‑1 (MQ=255)
                                                                                                                                tACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTcc              <  2:726396/141‑1 (MQ=17)
                                                                                                                                 aCCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAGCGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATAGCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCt             <  1:669933/141‑1 (MQ=12)
                                                                                                                                   cTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATACACGCATATGCTTGATGTTTATCTCGTTGCTTCCGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGCTATAAATAGCTCCATTTTCCttt           <  2:382814/141‑1 (MQ=11)
                                                                                                                                   cTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCttt           >  2:430388/1‑141 (MQ=17)
                                                                                                                                   cTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCttt           <  1:445327/141‑1 (MQ=17)
                                                                                                                                    ttttGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCtttt          >  2:52010/1‑141 (MQ=32)
                                                                                                                                     tttGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATTTAAATACCAACATTTTTCttttt         >  1:522290/1‑140 (MQ=11)
                                                                                                                                     tttGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACACATATGCTGAATGGTTTTCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCttttt         >  1:369911/1‑140 (MQ=11)
                                                                                                                                       tGAGGTTTACCAAATTGTTTAATGAGACGGTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATAACTAATGTATGTCACTCTGCATCAATGGCACGAGATAAAAAGCTCCACTTTCCTTTTAtt       >  2:308156/1‑141 (MQ=11)
                                                                                                                                        gAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGACACGATATAAATAGCTCCATTTTCCTTTTAttt      >  2:249638/1‑141 (MQ=17)
                                                                                                                                            tttACCAAATTGTTTAATGAGGCGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGAt  <  1:585556/141‑1 (MQ=17)
                                                                                                                                            |                                                                                                                                             
ATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAGCCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGAT  >  CP000730/36034‑36314

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: