BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP000730	36,315	G→A	56.1%	Y79Y (TAC→TAT)	USA300HOU_0026 ←	IS431mec transposase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	36,315	0	G	A	56.1%	144.0 / inf	744	Y79Y (TAC→TAT)	USA300HOU_0026	IS431mec transposase
Reads supporting (aligned to +/- strand): ref base G (148/171); new base A (189/220); total (348/396)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 748 total aligned reads displayed.

TTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATAT  >  CP000730/36175‑36452

ttACCAAATTGTTTAATGAGACGTTTGATAAACGCGTATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATg                                                                                                                                           <  2:384264/141‑1 (MQ=17)

    cAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGAAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGAAATAAATGGCCCCATTTTCCTTTTATTTTGATGTAct                                                                                                                                       >  2:353089/1‑140 (MQ=255)

       aTTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTTATATACGtct                                                                                                                                    >  1:230507/1‑141 (MQ=12)

        ttGTTTAACTAGACGTTTGATAAACGCCGATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGtctc                                                                                                                                   <  2:482790/141‑1 (MQ=11)

          gTTTAATGAGCCGTTTGATAAAAGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCtcat                                                                                                                                 <  2:715906/141‑1 (MQ=255)

           tttAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCtcatc                                                                                                                                <  1:751221/141‑1 (MQ=32)

              aaTGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAAt                                                                                                                             >  2:762878/1‑141 (MQ=17)

               aTGAGACGTTTGATAACCGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATa                                                                                                                            <  2:52582/141‑1 (MQ=12)

               aTGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCTTCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTAATCAATa                                                                                                                            >  1:155592/1‑141 (MQ=17)

                tGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATAc                                                                                                                           <  2:100688/141‑1 (MQ=12)

                tGAGACGTGTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATAc                                                                                                                           <  2:697262/141‑1 (MQ=12)

                   gtcGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGcc                                                                                                                        <  1:295833/139‑1 (MQ=12)

                   gaCGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCCCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGcc                                                                                                                        <  1:638944/141‑1 (MQ=255)

                   gaCGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGcc                                                                                                                        >  2:372463/1‑141 (MQ=32)

                    aCGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTCTTTTGATGTACGTCTCATGAATACGcga                                                                                                                       >  1:209518/1‑139 (MQ=12)

                     cGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCAt                                                                                                                      <  2:629934/141‑1 (MQ=32)

                       tttGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCTCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACTTCTCATCAAAACGCCGttt                                                                                                                    >  1:345043/1‑141 (MQ=11)

                       tttGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCAttt                                                                                                                    >  1:736075/1‑141 (MQ=32)

                           aTAAACGCATATGCTGAATGATTATCTCGTTGCTTACGAAACTAAATATCTAATGTATGTCCCTCTGCATCAATGGCCCGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGtaa                                                                                                                <  2:272038/141‑1 (MQ=12)

                              aaCGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATTTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGtaataa                                                                                                             <  1:304157/141‑1 (MQ=255)

                                cGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATGCGCAATTTGtaatgacc                                                                                                           >  2:363743/1‑137 (MQ=11)

                                  cATACGCTGAATGACTATCTCGTTGCTTACGCAACCAAATATCTAATTTATGTCTCTCTGCATCAATGGCACGATAAAAATAGCTCGATTTGCCCTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCtt                                                                                                         <  1:338142/141‑1 (MQ=11)

                                        ttGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTAt                                                                                                   <  2:392852/140‑1 (MQ=255)

                                        cTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTAt                                                                                                   <  2:246739/141‑1 (MQ=255)

                                        cTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTTATGTACGTCTCATCAATCGGCCGTTTGTGATAAGGTTTTTTAt                                                                                                   >  1:196291/1‑141 (MQ=11)

                                         tGAATGATTATCTCTTTGCTTACGCAACGAAATATCTAATGTAGGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCGCATCAATACGACATTTGTAATAAGCTTTTTTATg                                                                                                  <  1:172752/141‑1 (MQ=14)

                                            aTGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATCTGTAATAAGCTTTTTTATGCtt                                                                                               >  1:461998/1‑141 (MQ=255)

                                              gATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCtttt                                                                                             <  1:705285/141‑1 (MQ=40)

                                                 tagctcGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGAGATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTttct                                                                                          <  1:512993/138‑1 (MQ=255)

                                                  aTCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTTATATACGTCTCATCAATCCGCCATTTGTAATAAGCTTTTTTATGCTTTttctt                                                                                         >  1:56220/1‑141 (MQ=255)

                                                  aTCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTttctt                                                                                         >  2:734863/1‑141 (MQ=40)

                                                   tctcGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTGATAAGCTTTGTTTTTCTTTttgttc                                                                                        >  1:323175/1‑141 (MQ=11)

                                                   tctcGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTttcttc                                                                                        <  2:553675/141‑1 (MQ=40)

                                                   tctcCTTGTTTACGTAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTttcttc                                                                                        <  1:742063/141‑1 (MQ=255)

                                                      cGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCaa                                                                                     >  2:434662/1‑141 (MQ=255)

                                                        ttGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATTAATACGCCATTTGTCATAAGCTTTTTTATGTTTTTTCTTTCAAAt                                                                                   >  1:406045/1‑141 (MQ=14)

                                                          gcgtaagCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATATGCCATTTGTAATAAGCTTTTTTAGGTTTTttcttcgagtttt                                                                                 >  1:247091/7‑134 (MQ=14)

                                                           cTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTg                                                                                >  1:766673/1‑141 (MQ=255)

                                                            ttACGCAGCCAAATATCTAATGTAGGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCGTTTTTATGCTTTTTCTTCGAACTTTGa                                                                               >  1:492685/1‑141 (MQ=11)

                                                             tACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGAt                                                                              >  1:668174/1‑141 (MQ=255)

                                                               cGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGATCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGGAATAAGCTTTTTTATGCTGTTTCTTCCAAATTTGATat                                                                            <  1:431904/141‑2 (MQ=14)

                                                                gCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACa                                                                           <  1:27189/141‑1 (MQ=255)

                                                                 cAACTAAATATTTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATataa                                                                          <  1:11415/141‑4 (MQ=255)

                                                                 cAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTATTAAGCTTTTTTATGCTTTTTCTTGAAAATTTGATATaa                                                                          >  1:54128/1‑141 (MQ=11)

                                                                 cAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATataa                                                                          <  1:146866/141‑4 (MQ=255)

                                                                 cAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTACAATTATGATATaa                                                                          >  1:39337/1‑141 (MQ=11)

                                                                 cAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATataa                                                                          <  1:372463/141‑4 (MQ=38)

                                                                 cAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATataa                                                                          <  2:255991/141‑4 (MQ=38)

                                                                   aCCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATaaaa                                                                        >  2:587379/1‑141 (MQ=38)

                                                                     cAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATTTAAAAtt                                                                      >  2:128174/1‑141 (MQ=255)

                                                                     cAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGTTATAAAAtt                                                                      >  2:541356/1‑141 (MQ=255)

                                                                       aaTATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCTTCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTAATATAAACTTgg                                                                    >  1:599078/1‑141 (MQ=255)

                                                                        atatTTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTggg                                                                   <  2:677318/141‑1 (MQ=11)

                                                                         tatCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTTATAAGCTTTTTTATGCTTTTTCTTTCGAATTTGATATAAAATTcggg                                                                  >  2:282374/1‑141 (MQ=11)

                                                                          ttCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGc                                                                 <  2:322150/140‑1 (MQ=38)

                                                                            cTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTACTAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCat                                                               <  2:706705/141‑1 (MQ=255)

                                                                             tAATGTATGTCCCTCTGCTTCATTGTCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTTTGCTTTTTCTTCCAAATTTGATATAAAATTGGTGCagt                                                              >  1:569843/1‑139 (MQ=11)

                                                                                  tatgtCCCGCAGCATCAATGGCACGATATACATAGCTCCATGTTCCTTTTATCTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTTTTCCAAAGGGGATACAAAATTGGGGCATATTCtt                                                         <  1:183435/141‑1 (MQ=14)

                                                                                   atgtCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTg                                                        <  1:479277/141‑1 (MQ=255)

                                                                                     gtCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGaa                                                      <  1:695399/141‑1 (MQ=255)

                                                                                      tCCCTCTGCATCAATGGAACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTACTAAGCTTTTTTATGCTTTTTCTTCAAATTTTGATATAAAATTGGGGCATATTATTGAAc                                                     >  2:618091/1‑141 (MQ=11)

                                                                                        ccTTTGTATCATTGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGGCTCCTCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAGATTTGATATAAAATTGGGGCATATTCTTGAAccc                                                   <  2:508419/141‑1 (MQ=11)

                                                                                             ttaTCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACgg                                              <  2:470077/139‑1 (MQ=255)

                                                                                              taTCAATTGCACCATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGt                                             <  2:602676/140‑1 (MQ=255)

                                                                                                 cAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTGTGCCAATTTTGTTACAAAATGGGGGCAGTTTCTTGAAGCCAACgtttga                                          >  1:582762/1‑136 (MQ=14)

                                                                                                 cAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGa                                          <  1:686122/141‑1 (MQ=18)

                                                                                                  aaTGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGAc                                         >  2:687694/1‑141 (MQ=21)

                                                                                                      gCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGtt                                     <  2:746787/141‑1 (MQ=40)

                                                                                                       cACGATATAAATAGCACCATTTTACTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTg                                    >  2:164645/1‑141 (MQ=11)

                                                                                                          gATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCACCGGTAGATCGTTGAat                                 >  1:103599/1‑141 (MQ=255)

                                                                                                          gATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGCGGCATATTCTTGAACCCAACGGTAGATCGTTGAat                                 >  1:663463/1‑141 (MQ=255)

                                                                                                           atatAAATATCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAatg                                <  2:497936/141‑1 (MQ=255)

                                                                                                           atatAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCCACGGTAGATCGTTGAatg                                >  2:517524/1‑141 (MQ=255)

                                                                                                           atatAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGActg                                >  2:462260/1‑141 (MQ=255)

                                                                                                           atatAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAatg                                <  1:658502/141‑1 (MQ=255)

                                                                                                            tataAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAatga                               <  2:170993/141‑1 (MQ=18)

                                                                                                             ataAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAatgat                              <  1:618476/141‑1 (MQ=255)

                                                                                                              taAATAGCTCCATTATCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAatgatg                             >  1:414279/1‑141 (MQ=255)

                                                                                                                aaTAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGACATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAAGTTGATTTAAAATTGTGGCATGTTCTTGAACCCAACGTTAGATCGTTTAATGATGaa                           >  2:448870/1‑141 (MQ=11)

                                                                                                                 aTAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGCTCGTTGAATGATGAAc                          >  1:386481/1‑141 (MQ=255)

                                                                                                                 aTAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAAc                          <  1:451907/141‑1 (MQ=255)

                                                                                                                     cTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGttt                      <  2:663111/141‑1 (MQ=18)

                                                                                                                      tCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTa                     >  1:532866/1‑141 (MQ=21)

                                                                                                                       ccATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTac                    <  2:80605/141‑1 (MQ=39)

                                                                                                                       ccATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTac                    <  2:415251/141‑1 (MQ=255)

                                                                                                                       ccATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAAATCGTTGAATGATGAACGTTTac                    >  2:14432/1‑141 (MQ=255)

                                                                                                                            ttCCTTTTATTTTGATTGTGGTTTCATCAATACGCCATTTGTAAGAAGCTTTTTTATGCTTTTTCTTCCAAATTTGACATAAAATTGTGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTAcaccac               <  1:724397/141‑1 (MQ=11)

                                                                                                                            ttCCTTTTATTTTGATATACGTCTCATCAATACGCCAGTTGTAATAAGCTTTTTTAAGCTTTTTGTTCCAACTTTGTTATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTAcaccac               <  2:301461/141‑1 (MQ=11)

                                                                                                                             tCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTTTAATAATCTTTTTTATGCTTTTGCTTCCAAATTTGATATAAAATTTGGGCATATTCTTGAACCCATCGGTAGATCGTTGAATGATGAACGTTTACACCACg              <  2:154048/141‑1 (MQ=11)

                                                                                                                             tCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGTGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACg              >  2:425337/1‑141 (MQ=255)

                                                                                                                             tCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCCTATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTGTGCACCACg              >  1:427538/1‑141 (MQ=11)

                                                                                                                               cTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGtt            >  2:201639/1‑141 (MQ=21)

                                                                                                                               cTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGtt            >  2:572255/1‑141 (MQ=255)

                                                                                                                                ttttATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTc           >  1:358346/1‑141 (MQ=21)

                                                                                                                                 tttATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTct          >  1:86795/1‑140 (MQ=255)

                                                                                                                                    ttttgGATATACGTCTCAGTGACGCGGCATTTGTAACAAGCTTGTTTTCGCTTTGTCTGCCAAATGTGATATAGGTGTGGGGCGTATGCTGGAACCGAACGGTAGTTCGTTGAATGATGAACGTTTACACTACGTTCTCtt       <  2:213547/140‑1 (MQ=2)

                                                                                                                                    aatttGATGGACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCtt       <  2:340933/139‑1 (MQ=255)

                                                                                                                                    aTTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCtt       <  1:129741/141‑1 (MQ=11)

                                                                                                                                     ttttGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTa      <  1:712088/141‑1 (MQ=11)

                                                                                                                                         gATGTACGTCTCATCAATATGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAatat  >  2:654021/1‑141 (MQ=25)

TTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATAT  >  CP000730/36175‑36452

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`