Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 873,442 A→G 5.1% E116G (GAA→GGA)  clfA → fibrinogen‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP000730873,4420AG 5.1% 222.6 / 3.0 78E116G (GAA→GGA) clfAfibrinogen‑binding protein
Reads supporting (aligned to +/- strand):  ref base A (33/41);  new base G (2/2);  total (35/43)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.43e-01

CGACACAAACGTGAGTGATACTAAAACATCGTCAAACACTAATAATGGCGAAACGAGTGTGGCGCAAAATCCAGCACAACAGGAAACGACACAATCATCATCAACAAATGCAACTACGGAAGAAACGCCGGTAACTGGTGAAGCTACTACTACGACAACGAATCAAGCTAATACACCGGCAACAACTCAATCAAGCAATACAAATGCGGAGGAATTAGTGAATCAAACAAGTAATGAAACGACTTCTAATGATACTAATACAGTATCATCTGTAAATTCAC  >  CP000730/873302‑873582
                                                                                                                                             |                                                                                                                                            
cgacACAAACGTGAGTGATACTAAAACATCGTCAAACACTAATAATGGCGAAACGAGTGTGGCGCAAAATCCAGCACAACAGGAAACGACACAATCATCATCAACAAATGCAACTACGGAAGAAACGCCGGTAACTGGTGa                                                                                                                                              <  1:53645/141‑1 (MQ=255)
 gacACAAACGTGAGTGATACTAAAACATCGTCAAACACTAATAATGGCGAAACGAGTGTGGCGCAAAATCCAGCACAACAGGAAACGACACAATCATCATCAACAAATGCAACTACGGAAGAAACGCCGGTAACTGGTGaa                                                                                                                                             <  1:252237/141‑1 (MQ=255)
    acaAACGTGAGTGATACTAAAACATCGTCAAACACTAATAATGGCGAAACGAGTGTGGCGCAAAATCCAGCACAACAGGAAACGACACAGTCATCATCAACAACTGCAACTACGGAAGAAGCGCCGGTAACTGGTGAAGct                                                                                                                                          >  1:599456/1‑141 (MQ=255)
     caAACGTGAGTGATACTAAAACATCGTCAAACACTAATAATGGCGAAACGAGTGTGGCGCAAAATCCAGCACAACAGGAAACGACACAATCATCATCAACAAATGCAACTACGGAAGAAACGCCGGTAACTGGTGAAGcta                                                                                                                                         >  2:387551/1‑141 (MQ=255)
      aaaCGTGAGTGATACTAAAACATCGTCAAACACTAATAATGGCGAAACGAGTGTGGCGCAAAATCCAGCACAACAGGAAACGACCCCATCATCATCAACAAATGCAACTACGGAAGAAACGCCGGTAACTGGTGAAGctac                                                                                                                                        <  2:86926/141‑1 (MQ=255)
      aaaCGTGAGGTATACGAAAACATCGCCAAACACTAATAATGGCGAAACGAGTGTGGCGCAAAATCCAGCACAACAGGAAACGACACAATCATCATCAACAAATGCAACTACGGAAGAAACGCCGGTAACTGGTGAAGctac                                                                                                                                        <  2:346563/141‑1 (MQ=255)
        aCGTGAGTGATACTAAAACATCGTCAAACACTAATAATGGCGAAACGAGTGTGGCGCAAAATCCAGCACAACAGGAAACGACACAATCATCATCAACAAATGCAACTACGGAAGAAACGCCGGTAACTGGTGAAGctacta                                                                                                                                      <  1:523403/141‑1 (MQ=255)
             agtgaTACTAAAACATCGTCAAACACTAATAATGGCGAAACGAGTGTGGCGCAAAATCCAGCACAACAGGAAACGACACAATCATCATCAACAAATGCAAATACGGTAGCAACGCCGGGAAGTAGTGAAGCTGATACTACg                                                                                                                                 >  1:759565/1‑141 (MQ=255)
              gtgaTACTAAAACATCGTCAAACACTAATAATGGCGAAACGAGTGTGGCGCAAAATCCAGCACAACAGGAAACGACACAATCATCATCAACAAATGCAACTACGGAAGAAACGCCGGTAACTGGTGAAGCTACTACTACGa                                                                                                                                <  1:358313/141‑1 (MQ=255)
                 aTACTAACACATCACCAAACACCAATAATGGCGAAACGAGTGTTGCGCAAAATCCACCACAACAGGAAACGACACAATCATCATCAACAAATGCAACTACGGAAGAAACGCCGGTAACTGGTGAAGCTACTACTACGACaa                                                                                                                             <  1:598/141‑1 (MQ=255)
                   aCTAAAACATCGTCAAACACTAATAATGGCGAAACGAGTGTGGCGCAAAATCCAGCACAACAGGAAACGACACAATCATCATCAACAAATGCAACTACGGAAGAAACGCCGGTAACTGGTGAAGCTACTACTACGACAACg                                                                                                                           <  1:214900/141‑1 (MQ=255)
                   aCTAAAACATCGGCAAACACTAATAATGGCGAAACGAGTGTGGCGCAAAATCCAGCACAACAGGAAACGACACAATCATCATCAACAAAGGCAACTACGGAAGAAACGCCGGTAACTGGTGAAGCTACTACTACGACAACg                                                                                                                           <  2:330973/141‑1 (MQ=255)
                          cATCGTAAAACACTAATAATGGCGAAACGAGTGTGGCGCAAAATCCAGCACAACATGAAACGACACAATCATCATCAACAAATGCAACGACGGAAGAAACGCCGGTAACTGGTGAAGCTACTACTACGACAACGAATCAAg                                                                                                                    >  2:486986/1‑141 (MQ=255)
                              ggcAAACACTAATAATGGCGAAACGAGTGTGGCGCAAAATCCAGCACAACAGGAAACGACACAATCATCATCAACAAATGCAACTACGGAAGAAACGCCGGTAACTGGTGAAGCTACTACTACGACAACGAATCAAGCTaa                                                                                                                <  1:348072/139‑1 (MQ=255)
                              gTCAAACACTAATAATGGCGAAACGAGTGTGGCGCAAAATCCAGCACAACAGGAAACGACACAATCATCATCAACAAATGCAACTACGGAAGAAACGCCGGTAACTGGTGAAGCTACTACTACGACAACGAATCAAGCTaa                                                                                                                >  1:769229/1‑141 (MQ=255)
                                cAAACACTAATAATGGCGAAACGAGTGTGGCGCAAAATCCAGCACAACAGGAAACGACACAATCATCATCAACAAATGCAACTACGGAAGAAACGCCGGTAACTGGTGAAGCTACTACTACGACAACGAATCAAGCTAATa                                                                                                              >  1:239018/1‑141 (MQ=255)
                                   aaccTAATAATGGCGAAACGAGTGTGGCGCAAAATCCAGCACAACAGGAAACGACACAATCATCATCAACAAATGCAACTACCGAAGAAACGCCGGTAACTGGTGAAGCTACTACTACGACAACGAATCAAGCTAATacac                                                                                                           <  2:477292/138‑1 (MQ=255)
                                        aataatGGCGAAACGAGTGAGACGCAAAATCCAGCCAGAAAGGAAGTTCCCCATCCGTCATCAACAAATGCAACTACGGAAGAAACGCCGGTAACTGGTGAAGCTACTACTACGACAACGAATCAAGCTAATACACCGGca                                                                                                      <  1:363789/141‑1 (MQ=255)
                                          taatGGCGAAACGAGTGTGGCGCAAAATCCAGCACAACAGGAAACGACACAATCATCATCAACAAATGCAACTACGGAAGAAACGCCGGTAACTGGTGAAGCTACTACTACGACAACGAATCAAGCTAATACACCGGcaac                                                                                                    <  1:63911/141‑1 (MQ=255)
                                          taatGGCGAAACGAGTGTGGCGCAAAATCCACCACAACAGTAAACGACACAATCATCAACCACAACTCCAACTACGGAAGAAACACCGGAAAATGGTAAAGCTAATACTACGACAACCAATCAAGCAAATGCACCGGcagc                                                                                                    >  2:229654/1‑139 (MQ=255)
                                            atGGCGAAACGAGTGTGGCGCAAAATCCAGCACAACAGGAAACGACACAATCATCATCAACAAATGCAACTACGGAAGAAACGCCGGTAACTGGTGAAGCTACTACTACGACAACGAATCAAGCTAATACACCGGcaacaa                                                                                                  <  2:114624/141‑1 (MQ=255)
                                            atGGCGAAACGAGTGTGGCGCAAAATCCAGCACAACAGGAAACGACACAATCATCATCAACAAATGCAACTACGGAAGAAACGCCGGTAACTGGTGAAGCTACTACTACGACAACGAAACAAGCTAATACACAGGaaaaaa                                                                                                  >  2:462037/1‑141 (MQ=255)
                                            acggCGAAACCAGTGTGGCGCAAAATCCAGCACAACAGGAAACGACAAAATCATCATCAACAAATGCACCTACGAAAGAAACGCCGGTAACTCGTGAAGCTACTACTACGATAACGAATCAAGCTAATACACCGGcaacaa                                                                                                  <  2:221377/139‑1 (MQ=255)
                                             tGGCGAAACGAGTGTGGCGCAAAATCCAGCACAACAGGCACCGCTCACAGCATCAGCAACAAATGCAACTACGGAAGAAACGCCGGTAACTGGTGAAGCTACTACTACGACAACGAATCAAGCTAATACACCGGCaacaac                                                                                                 <  1:250981/141‑1 (MQ=255)
                                             tGGCGAAACGAGTGTGGCGCAAAATCCAGCACAACAGGAAACGACACAATCATCATCAACAAATGCAACTACGGAAGAAACGCCGGTAACTGGTGAAGCTACTACTACGACAACGTATCAAGCTATTACACCGGCaacaac                                                                                                 <  1:387418/141‑1 (MQ=255)
                                              ggCGAAACGAGTGTGGCGCAAAATCCAGCACAACAGGAAACGACACAATCATCATCAACAAATGCAACTACGGAAGAAACGCCGGTAACTGGTGAAGCTACTACTACGACAACGAATCAAGCTAATACACCGGCAACAACt                                                                                                <  1:387551/141‑1 (MQ=255)
                                              ggCGAAACGAGTGTGGCGCAAAATCCAGCACAACAGGAAACGACACAATCATCATCAACAAATGCAACTACGGAAGAAACGCCGGTAACTGGTGAAGCTACTACTACGACAACGAATCAAGCAAATACACCGGCaacaacc                                                                                                >  1:135723/1‑140 (MQ=255)
                                              ggCGAAACGAGTGTGGCGCAAAACCCAGCACAACAGGAAACGACACAATCATCATCAACAAATGCAACCACGGAAGAAACGCCGGTAACTGGTGAAGCTACTACTACGACAACGAATCAAGCTAATACACCGGCAACAACt                                                                                                <  2:252635/141‑1 (MQ=255)
                                                   aaCGAGTGTGGCGCAAAATCCAGCACAACAGGAAACGACACAATCATCATCAACAAATGCAACTACGGAAGAAACGCCGGTAACTGGTGGAGCTACTACTACGACAACGAATCAAGCTAATACACCGGCAACAACtcaatc                                                                                           >  1:329505/1‑141 (MQ=255)
                                                   aaCGAGTGTGGCGCAAAATCCAGCACAACAGGAAACGACACAATCATCATCAACAAATGCAACTACGGAAGAAACGCCGGTAACTGGTGGAGCTACTACTACGACAACGAATCAAGCTAATACACCGGCAACAACtcaatc                                                                                           >  2:328643/1‑141 (MQ=255)
                                                   aaCGAGTGTGGCGCAAAATCCAGCACAACAGGAAACGACACAATCATCATCAACAAATGCAACTACGGAAGAAACGCCGGTAACTGGTGAAGCTACTACTACGATAACGAATCAAGCTAATACACCGGCAACAACtcaatc                                                                                           >  2:143928/1‑141 (MQ=255)
                                                   aaCGAGTGTGGCGCAAAATCCAGCACAACAGGAAACGACACAATCATCATCAACAAATGCAACTACGGAAGAAACGCCGGTAACTGGTGAAGCTACTACTACGACAACGAATCAAGCTAATACACCGGCACCAACtcaata                                                                                           >  2:641861/1‑140 (MQ=255)
                                                     cGAGTGTGGCGCAAAATCCAGCACAACAGGAAACGACACAATCATCATCAACAAATGCAACTACGGAAGAAACGCCGGTAACTGGTGAAGCTACTACTACGACAACGAATCAAGCTAATACACCGGCAACAACtcaatcaa                                                                                         >  1:260765/1‑141 (MQ=255)
                                                      gAGTGTGGCGCAAAATCCAGCACAACAGGAAACGACACAATCATCATCAACAAATGCAACTACGGAAGAAACGCCGGTAACTGGTGAAGCTACTACTACGACAACGAATCAAGCTAATAAACCGGCAACAACTCAATCAAg                                                                                        >  2:158956/1‑141 (MQ=255)
                                                        gtgtGGCGCAAAATCCAGCACAACAGGAAACGACACAATCATCATCAACAAATGCAACTACGGAAGAAACGCCGGTAACTGGTGAAGCTACTACTACGACAACGAATCAAGCTAATACACCGGCAACCACTCAATCAAGCa                                                                                      >  1:42012/1‑141 (MQ=255)
                                                            ggCGCAAAATCCAGCACAACAGGAAACGACACAATCATCATCAACAAATGCAACTACGGAAGAAACGCCGGTAACTGGTGAAGCTACTACTACGACAACGAATCAAGCTAATACACCGGCAACAACTCAATCAAGCAATAc                                                                                  <  1:51572/141‑1 (MQ=255)
                                                            ggCGCAAAATCCAGCACAACAGGAAACGACACAATCATCATCAACAAATGCAACTACGGAAGAAACGCCGGTAACTGGTGAAGCTACTACTACGACAACGAATCAAGCTAATACACCGGCAACAACTCAATCAAGCAATAc                                                                                  <  1:124428/141‑1 (MQ=255)
                                                             gcgcAAAATCCAGCACAACAGGAAACGACACAATCATCATCAACAAATGCAACTACGGAAGAAACGCCGGTAACTGGTGAAGCTACTACTACGACAACGAATCAAGCTAATACACCGGCAACAACTCAATCAAGCAATACa                                                                                 <  1:501090/141‑1 (MQ=255)
                                                             gcgcAAAATCCAGCACAACAGGAAACGACACAATCATCATCAACAAATGCAACTACGCAAGAAACGCCGGTAACTGGTGAAGCTACTACTACGACAACGAATCAAGCTAATACACCGGCAACAACTCAATCAAGCAATACa                                                                                 <  2:746160/141‑1 (MQ=255)
                                                               gcAAAATCCAGCACAACAGGAAACGACACAATCATCATCAACAAATGGAACTACGGAAGAACCGCCGGTAACTGGTGAAGCTACTACTACGACAACGAATCAAGCTAATACACCGGCAGAAACTCAATCGAGCAGTACaaa                                                                               >  1:217968/1‑141 (MQ=255)
                                                               gcAAAATCCAGCACAACAGGAAACGACACAATCATCATCAACAAATGCAACTACGGAAGAAACGCCGGTAACTGGTGAAGCTACTACTACGACAACGAATCAAGCTAATACACCGGCAACAACTCAATCAAGCAATACaaa                                                                               >  1:477180/1‑141 (MQ=255)
                                                                cAAAATCCAGCACAACAGGAAAAGACACAATCATCATCAACAAATGCAACTACGGAAGAAACGCCGGTAACTGGTGAAGCTACTACTACGACAACGAAGCAAGCTAATACACCGGCAACAACTCAATCAAGCAATACAAAt                                                                              <  2:340207/141‑1 (MQ=255)
                                                                   aaTCCAGCACAACAGGAAACGACACAATCATCATCAACAAATGCAACTACGGAAGAAACGCCGGTAACTGGTGAAGCTACTACTACGACAACGAATCAAGCTAATACACCGGCAACAACTCAATCAAGCAATACAAATGCg                                                                           <  1:12767/141‑1 (MQ=255)
                                                                     tCCAGCACAACAGGAAACGACACAATCATCATCAACAAATGCAACTACGGAAGAAACGCCGGTAACTGGTGAAGCTACTACTACGACAACGAATCAAGCTAATACACCGGCAACAACTCAATCAAGCAATACAAATGCgga                                                                         <  1:509569/141‑1 (MQ=255)
                                                                      ccAGCACAACAGGAAACGACACAATCATCATCAACAAATGCAACTACGGAAGAAACGCCGGTAACTGGTGAAGCTACTACTACGACAACGAATCAAGCTAATACACCGGCAACAACTCAATCAAGCAATACAAATGCggag                                                                        >  1:560569/1‑141 (MQ=255)
                                                                        aGCACAACAGGAAACGACACAATCATCATCAACAAATGCAACTACGGAAGAAACGCCGGTAACTGGTGAAGCTACTACTACGACAACGAATCAAGCTAATACACCGGCAACAACTCAATCAAGCAATACAAATGCggagga                                                                      <  1:272629/141‑1 (MQ=255)
                                                                              acaGGAAACGACACAATCATCATCAACAAATGCAACTACGGAAGAAACGCCGGTAACTGGTGAAGCTACTACTACGACAACGAATCAAGCTAATACACCGGCAACAACTCAATCAAGCAATACAAATGCGGAGGAAtaggg                                                                >  2:222163/1‑137 (MQ=255)
                                                                              acaGGAAACGACACAATCATCATCAACAAATGCAACTACGGAAGAAACGCCGGTAACTGGTGAAGCTACTACTACGACAACGAATCAAGCTAATACACCGGCAACAACTCAATCAAGCAATACAAAGGCGGAGGAATAAgg                                                                >  1:230481/1‑140 (MQ=255)
                                                                               caGGAAACGACACAATCATCATCAACAAATGCAACTACGGAAGAAACGCCGGTAACTGGTGAAGCTACTACTACGACAACGAATCAAGCTAATACACCGGCAACAACTCAATCAAGCAATACAAATACGGAGGAATAAGTg                                                               >  2:586735/1‑141 (MQ=255)
                                                                                  gAAACGACACAATCATCATCAACAAATGCAACTACGGAAGAAACGCCGGTAACTGGTGAAGCTACTACTACGACAACGAATCAAGCTAATACACCGGCAACAACTCAATCAAGCAATACAAATGCGGAGGAATTAGTGAAt                                                            <  1:143004/141‑1 (MQ=255)
                                                                                    aaCGACACAAACAACAGCAACAAATGCAACTACGGAAGAAACGCCGGTAACTGGTGAAGCTACTACTACGACAACGAATCAAGCTAATACACCGGCAACAACTCAATCAAGCAATACAAATGCGGAGGAATTAGTGAATCa                                                          <  2:169762/141‑1 (MQ=255)
                                                                                       gACACAATCATCATCAACAAATGCAACTACGGAAGAAACGCCGGTAACTGGTGAAGCTACTACTACGACAACGAATCAAGCTAATACACCGGCAACAACTCAATCAAGCAATACAAATGCGGAGGAATTAGTGAATCAAAc                                                       <  1:316845/141‑1 (MQ=255)
                                                                                       gACACAATCATCATCAACAAATGCAACTACGGAAGAAACGCCGGTAACTGGTGAAGCTACTACTACGACAACGAATCAAGCTAATACACCGGCAACAACTCAATCAAGCAATACAAATGCGGAGGAATTAGTGAATCAAAc                                                       <  1:443767/141‑1 (MQ=255)
                                                                                         cacaATCATCATCAACAAATGCAACTACGGAAGAAACGCCGGTAACTGGTGAAGCTACTACTACGACAACGAATCAAGCTAATACACCGGCAACAACTCAATCAAGCAATACAAATGCGGAGGAATTAGTGAATCAAACaa                                                     <  2:560569/141‑1 (MQ=255)
                                                                                         cacaATCATCATCAACAAATGCAACTACGGAAGAAACGCCGGTAACTGGTGAAGCTACTACTACGACAACGAATCAAGCTAATACACCGGCAACAACTCAATCAAGCAATACAAATGCGGAGGAATTAGTGAATCAAACaa                                                     >  1:271989/1‑141 (MQ=255)
                                                                                         cacaATCATCATCAACAAATGCAACTACGGAAGAAACGCCGGTAACTGGTGAAGCTACTACTACGACAACGAATCAAGCTAATACACCGGCAACAACTCAATCAAGCAACACAAATGCGGAGGAATTAGTGAGTCAAACaa                                                     >  1:86335/1‑141 (MQ=255)
                                                                                         cacaATCATCATCAACAAATGCAACTACGGAAGAAACGCCGGGAACTGGTGAAGCTACTACTACGACAACGAATCAAGCTAATACACCGGCAACAACTCAATCAAGCAATACAAATGCGGAGGAATTAGTGAATCAAACaa                                                     >  2:386340/1‑141 (MQ=255)
                                                                                                  ccatcaACAAATGCAACTACGGAAGAAACGCCGGTAACTGGTGAATCTACTACTACGACAACGAATCAAGCTAATACACCGGCAACAACTCAATCAAGCAATACAAATGCGGAGGAATTATTGAATCAAACAAGTAATGaa                                             <  1:543522/140‑1 (MQ=255)
                                                                                                       aacaaATGCAACTACGGAAGAAACGCCGGTAACTGGTGAAGCTACTACTACGACAACAAATCAAGCTAATACACCGACAACAACTCAATCAAGCAATACAAATGCGGAGGAATTAGTGAAACAAACAAGTAATGAAACGaa                                        >  2:83917/1‑140 (MQ=255)
                                                                                                       aacaaACGCAACTACGGAAGAAACGCAGGTAACTGGTGAAGCTACTACTACGACAACGAATCAAGCTAATACACCGGCAACAACTCAATCAAGCAATACAAATGCGGAGGAATTAGTGAATCAAACAAGTAATGAAACGAc                                        <  1:319485/141‑1 (MQ=255)
                                                                                                         caaATGCAACTACGGAAGAAACGCCGGTAACTGGTGAAGCTACTACTACGACAACGAATCAAGCTAATACACCGGCAACAACTCAATCAAGCAATACAAATGCGGAGGAATTAGTGAATCAAACAAGTAATGAAACGACtt                                      <  1:536600/141‑1 (MQ=255)
                                                                                                          aaaTGCAACTACGGAAGAAACGCCGGTAACTGGTGGAGCTACTACTACGACAACGAATCAAGCTAATACACCGGCAACAACTCAATCAAGCAATACAAATGCGGAGGAATTAGTGAATCAAACAAGTAATGAAACGACTTc                                     <  1:328643/141‑1 (MQ=255)
                                                                                                          aaaTGCAACTACGGAAGAAACGCCGGTAACTGGTGGAGCTACTACTACGACAACGAATCAAGCTAATACACCGGCAACAACTCAATCAAGCAATACAAATGCGGAGGAATTAGTGAATCAAACAAGTAATGAAACGACTTc                                     <  2:329505/141‑1 (MQ=255)
                                                                                                           aaTGCAACTACGGAAGAAACGCCGGTAACTGGTGAAGCTACTACTACGACAACGAATCAAGCTAATGCAACGGCAACAACACAAGCCATTAATACAAATGCGGAGGAATTAGTGAATCAAACAAGTAATGAAACGACTTCt                                    <  1:180681/141‑1 (MQ=255)
                                                                                                            aTGCAACTACGGAAGAAACGCCGGTAACTGGTGAAGCTACTACTACGACAACGAATCAAGCTAATACACCGGCAACAACTCAATCAAGCAATACAAATGCGGAGGAATTAGTGAATCAAACAAGTAATGAAACGACTTCTa                                   <  2:769229/141‑1 (MQ=255)
                                                                                                             tGCAACTACGGAAGAAACGCCGGTAACTGGTGAAGCTACTACTACGACAACGAATCAAGCTAATACACCGGCAACAACTCCAGCAAGCAATACAAATGCGGAGGAATTAGTGAATCAAACAAGTAATGAAACGACTTCTaa                                  <  1:488455/141‑1 (MQ=255)
                                                                                                             tGCAACTACGGAAGAAACGCCGGTAACTGGTGAAGCTACTACTACGACAACGAATCAAGCTAATACACCGGCAACAACTCAATCAAGCAATACAAATGCGGAGGAATTAGTGAATCAAACAAGTAATGAAACGACTTCTaa                                  >  1:13388/1‑141 (MQ=255)
                                                                                                               cAACTACGGAAGAAACGCCGGTAACTGGTGAAGCTACTACTACGACAACGAATCAAGCTAATACACCGGCAACAACTCAATCAAGCAATACAAATGCGGAGGAATTAGTGAATCAAACAAGTAATGAAACGACTTCTAATg                                <  2:697331/141‑1 (MQ=255)
                                                                                                               cAACTACGGAAGAAACGCCGGTAACTGGTGAAGCTACTACTACGACAACGAATCAAGCTAATACACCGGCAACAACTCAATCAAGCAATACAAATGCGGAGGAATTAGTGAATCAAACAAGTAAAGAAACGACTTCTAATg                                >  1:609829/1‑141 (MQ=255)
                                                                                                                 aCTACGGAAGAAACGCCGGTAACTGGTGAAGCTACTACTACGACAACGGATCAAGCTAATACACCGGCAACAACTCAATCAAGCAATACAAATGCGGAGAAATTAGTGAAGCAAACAAGTAATGAACCGACTTCTAAtcaa                              >  1:285501/1‑138 (MQ=255)
                                                                                                                  cTACGGAAGAAACGCCGGTAACTGGTGAAGCTACTACTACGACAACGAATCAAGCTAATACACCGGCAACAACTCAATCAAGCAATACAAATGCGGAGGAATTAGTGAATCAAACAAGTAATGAAACGACTTCTAATGATa                             <  1:683644/141‑1 (MQ=255)
                                                                                                                        aagaaACGCCGGTAACTGGTGAAGCTACTACTACGACAACGAATCAAGCTAATACACCGGCAACAACTCAATCAAGCAATACAAATGCGGAGGAATTAGTGAATCAAACAAGTAATGAAACGACTTCTAATGATACTAATa                       <  1:286037/141‑1 (MQ=255)
                                                                                                                         agaaACGCCGGTAACTGGTGAAGCTACTACTACGACAACGAATCAAGCTAATACACCGTCAACAACTCAATCAAGCAATACAAATGCGGAGGAATTAGTGAATCAAACAAGTAATGAAACGACTTCTAATGATCCTAATAc                      <  1:487804/141‑1 (MQ=255)
                                                                                                                           aaaCGCCGGTAACTGGTGAAGCTACTACTACGACAACGAATCAAGCTAATACACCGGCAACAACTCAATCAAGCAATACAAATGCGGAGGAATTAGTGAATCAAACAAGTAATGAAACGACTTCTAATTATACTAATaacg                    >  2:361777/1‑138 (MQ=255)
                                                                                                                           aaaCGCCGGTAACTGGTGAAGCTACTACTACGACAACGAATCAAGCTAATACACCGGCAACAACTCAATCAAGCAATACAAATGCGGAGGAATTAGTGAATCAAACAAGTAATGAAACGACTTCTAATGATCCTAGTAAAg                    >  2:76599/1‑141 (MQ=255)
                                                                                                                             aCGCCGGTAACTGGTGAAGCTACTACTACGACAACGAATCAAGCTAATACACCGGCAACAACTCAATCAAGCAATACAAATGCGGAGGAATTAGTGAATCAAACAAGTAATGAAACGACTTCTAATGATACTAATACAGTa                  >  2:435426/1‑141 (MQ=255)
                                                                                                                               cccGGTAACTGGTGAAGCTAATACTACGACAACGAATCAAGCTAATCCACTGGCAACAACTCAATCAAGCAATACAAATGCGGAGGAATTAGTGAATCAAACAAGTAATGAAACGACTTCTAATGATACTAATACAGTatc                <  2:505444/140‑1 (MQ=255)
                                                                                                                                 cGGTAACTGGTGAAGCTACTACTACGACAACGAATCAAGCTAATACACCGGCAACAACTCAATCAAGCAATACAAATGCGGAGGAATTAGTGAATCAAACAAGTAATGAAACGACTTCTAATGATACTAATACAGTatcat              >  1:666105/1‑141 (MQ=255)
                                                                                                                                    tAACTGGTGAAGCTACTACTACGACAACGAATCAAGCTAATACACCGGCAACAACTCAATCAAGCAATACAAATGCGGAGGAATTAGTGAATCAAACAAGTAATGAAACGACTTCTAATGATACTAATACAGTATCATCTg           >  2:328456/1‑141 (MQ=255)
                                                                                                                                          gTGAAGCTACTACTACGACAACGAATCAAGCTAATACACCGGCAACAACTCAATCAAGCAATACAAATGCGGAGGAATTAGTGAATCAAACAAGTAATGAAACGACTTCTAATGATACTAATACAGTATCATCTGTAAAtt     >  2:206643/1‑141 (MQ=255)
                                                                                                                                             aaGCTACTACTACGACAACGAATCAAGCTAATACACCGGCAACAACTCAATCAAGCAATACAAATGCGGAGGAATTAGTGAATCAAACAAGTAATGAAACGACTTCTAATGATACTAATACAGTATCATCTGTAAATTCAc  <  2:477180/141‑1 (MQ=255)
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CGACACAAACGTGAGTGATACTAAAACATCGTCAAACACTAATAATGGCGAAACGAGTGTGGCGCAAAATCCAGCACAACAGGAAACGACACAATCATCATCAACAAATGCAACTACGGAAGAAACGCCGGTAACTGGTGAAGCTACTACTACGACAACGAATCAAGCTAATACACCGGCAACAACTCAATCAAGCAATACAAATGCGGAGGAATTAGTGAATCAAACAAGTAATGAAACGACTTCTAATGATACTAATACAGTATCATCTGTAAATTCAC  >  CP000730/873302‑873582

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: