Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 1,105,994 T→C 100% T12T (ACT→ACC)  pdhD → dihydrolipoyl dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007301,105,9940TC100.0% 234.8 / ‑6.5 69T12T (ACT→ACC) pdhDdihydrolipoyl dehydrogenase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base C (33/35);  minor base A (1/0);  total (34/35)
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

TTGACCACAGACAAATTGATGGTGCAACTGGCCAAAATGCAATGAATCACATTAAACGTTTATTAAATAATCCAGAATTATTATTAATGGAGGGGTAAAACATGGTAGTTGGAGATTTCCCAATTGAAACAGATACTATAGTAATCGGAGCAGGTCCTGGTGGATACGTTGCAGCAATTCGTGCAGCTCAATTAGGACAAAAAGTAACAATCGTTGAGAAAGGTAATCTTGGTGGTGTTTGCTTAAACGTAGGATGTATTCCTTCAA  >  CP000730/1105858‑1106124
                                                                                                                                        |                                                                                                                                  
ttGACCACAGACAAATTGATGGTGCAACTGGCCAAAATGCAATGAATCACATTAAACGTTTCTTAAATAATCCAGAATTATTATTAATGGAGGGGTAAAACATGGTAGTTGGAGATTTCCCAATTGAAACAGATACCATAg                                                                                                                                >  2:582739/1‑141 (MQ=255)
  gACCACAGACAAATTGATGGTGCAACTGGTCAAAATGCAATGAATCACATTAAACGTTTATTAAATAATCCAGAATTATTATTAATGGAGGGGTAAAACATGGTAGTTGGAGATTTCCCAATTGAAACAGATACCATAGTa                                                                                                                              <  2:660126/141‑1 (MQ=255)
    ccACAGACAAATTGATGGTGCAACTGGCCAAAATGCAATGAATCACATTAAACGTTTATTAAATAATCCAGAATTATTATTAATGGAGGGGTAAAACATGGTAGTTGGAGATTTCCCAATTGAAACGGAGACCATAGTaaa                                                                                                                            >  2:310381/1‑140 (MQ=255)
        aGACAAATTGATGGTGCAACTGGCCAAAATGCAATGAATCACATTAAACGTTTATTAAATAATCCAGAATTATTATTAATGGAGGGGTAAAACATGGTAGTTGGAGATTTCCCAATTGAAACAGATACCATAGTAATCGGa                                                                                                                        <  2:593698/141‑1 (MQ=255)
          aCAAATTGATGGTGCAACTGGCCAAAATGCAATGAATCACATGAAACGTTTATTAAATAATCCAGAATTATTATTAATGGAGGGGTAAAACATGGTAGTTGGAGATTTCCCAATTGAAACAGATACCATAGTAATCGGAGc                                                                                                                      >  2:582915/1‑141 (MQ=255)
              aTTGATGGTGCAACTGGCCAAAGTGCAATGAATCACATTAAACGTTTATTAAATAATCCAGAATTATTATTAATGGAGGGGTAAAACATGGTAGTTGGAGATTTCCCAATTGAAACAGATACCATAGTAATCGGAGCAGGt                                                                                                                  >  2:564900/1‑141 (MQ=255)
              aTTGATGGTGCAACTGGCCAAAATGCAATGAATCACATTAAACGTTTATTAAATAATCCAGAATTATTATTAATGGAGGGGTAAAACATGGTAGTTGGAGATTTCCCAATTGAAACAGATACCATAGTAATCGGAGCAGGt                                                                                                                  >  2:596224/1‑141 (MQ=255)
              aTTGATGGTGCAACTGGCCAAAATGCAATGAATCACATTAAACGTTTATTAAATAATCCAGAATTATTATTAATGGAGGGGTAAAACATGGTAGTTGGAGATTTCCCAATTGAAACAGATACCATAGTAATCGGAGCAGGt                                                                                                                  >  2:180018/1‑141 (MQ=255)
                 gATGGTGCAACTGGCCAAAATGCAATGAATCACATTAAACGTTTATTAAATAATCCAGACTTATTATTAATGGAGGGGTAAAACATGGTAGTTGGAGATTTCCCAATTGAAACAGATACCATAGTAATCGGAGCAGGTCCt                                                                                                               <  1:417787/141‑1 (MQ=255)
                 gATGGTGCAACTGGCCAAAATGCAATGAATCACATTAAACGTTTATTAAATAATCCAGAATTATTATTAATGGAGGGGTAAAACATGGTAGTTGGAGATTTCCCAATTGAAACAGATACCATAGTAATCGGAGCAGGTCCt                                                                                                               <  1:597399/141‑1 (MQ=255)
                       gCAACTGGCCAAAATGCAATGAATCACATTAAACGTTTATTAAATAATCCAGAATTATTATTAATGGAGGGGTAAAACATGGTAGTTGGAGATTTCCCAATTGAAACACATCCCCTCGTAATCGGCGCCGGTCCtggtggc                                                                                                         >  1:661721/1‑140 (MQ=255)
                       gCAACTGGCCAAAATGCAATGAATCACATTAAACGTTTATTAAATAATCCAGAATTATTATTAATGGAGGGGTAAAACATGGTAGTTGGAGATTGCCCAATTGAAACAGATACCATAGTAATCGGAGCAGGTCCTGGTGGa                                                                                                         <  1:693821/141‑1 (MQ=255)
                        cAACTGGCCAAAATGCAATGAATCACATTAAACGTTTATTAAATAATCCAGAATTATTATTAATGGAGGGGTAAAACATGGTAGTTGGAGATTTCCCAATTGAAACAGATACCATAGTAATCGGAGCAGGTCCTGGTGGAt                                                                                                        <  1:578326/141‑1 (MQ=255)
                          aCTGGCCAAAATGCAATGAATCACATTAAACGTTTATTAAATAATCCAGAATTATTATTAATGGAGGGGTAAAACATGGTAGTTGGAGATTTCCCAATTGAAACAGATACCATAGTAATCGGAGCCGGTCCTGGTGGAtcc                                                                                                      >  2:421314/1‑139 (MQ=255)
                             gggcacaaTGCAATGAATCACATTCAACGGTTATTAAATAATCCAGAATTATTATTAATGGAGGGGAAAAACATGGTATTAGGAGATTTCCCAATCGAAACAGATACCATAATCACGGGAGGAGGACGTGGTGTATActta                                                                                                   >  2:34820/7‑138 (MQ=255)
                               ccAAAATGCAATGAATCACATTAAACGTTTATTAAATAATCCAGAATTATTATTAATGGGGGGGTAAAACATGGTAGTTGGAGATTTCCCAATTGAAACAGATACCATAGTAATCGGAGCAGGTCCTGGTGGATAGGTTgc                                                                                                 >  1:130176/1‑141 (MQ=255)
                               ccAAAATGCAATGAATCACATTAAACGTTTATTAAATAATCCAGAATTATTATTAATGGAGGGGTAAAACATGGTAGTTGGAGATTTCCCAATTGAAACAGATACCATAGTAATCGGAGCAGGTCCTGGTGGATACGTTgc                                                                                                 >  1:258537/1‑141 (MQ=255)
                               ccAAAACGACATGAATCACATTAAACGTTTATTAAATAATCCAGAATTATTATTAATGGAGGGGTAAAACATGGTAGTTGGAGATTTCCCAATTGAAACAGATACCATAGTAATCGGAGCAGGTCCTGGTGGATACGTTgc                                                                                                 >  2:358223/1‑141 (MQ=255)
                                cAAAATGCAATGAATCACATTAAACGTTTATTAAATAATCCAGAATTATTATTAATGGAGGGGTAAAACATGGTAGTTGGAGATTTCCCAATTGAAACAGATACCATAGTAATCGGAGCAGGTCCTGGTGGATACGTTgca                                                                                                >  1:517010/1‑141 (MQ=255)
                                 aaaaTGCAATGAATCACATTAAACGTTTATTAAATAATCCAGAATTATTATTAATGGAGGGGTAAAACATGGTAGTTGGAGATTTCCCAATTGAAACAGATACCATAGTAATCGGAGCAGGTCCTGGTGGATACGTTgcag                                                                                               >  1:476792/1‑141 (MQ=255)
                                   aaTGCAATGAATCACATTAAACGTTTATTAAAGAATCCAGAATTATTATTAATGGAGGGGTAAAACATGGTAGTTGGAGATTTCCCAATTGAAACAGATACCATAGTAATCGGAGCAGGTCCTGGTGGATACGTTgcagca                                                                                             <  1:195294/141‑1 (MQ=255)
                                       cAATGAATCACATTAAACGTTTATTAAATAATCCAGAATTATTATTAATGGAGGGGTAAAACATGGTAGTTGGAGATTTCCCAATTGAAACAGATACCATAGTAATCGGAGCAGGTCCTGGTGGATACGTTGCAGCAATTc                                                                                         <  1:281100/141‑1 (MQ=255)
                                            aaTCACATTAAACGTTTATTAAATAATCCAGAATTATTATTAATGGAGGGGTAAAACATGGTAGTTGGAGATTTCCCAATTGAAACAGATACCATGGTAATCGGAGCAGGTCCTGGTGGATACGTTGCAGCAATTCGTGCa                                                                                    <  2:209948/141‑1 (MQ=255)
                                            aaTCACATTAAACGTTTATTAAATAATCCAGAATTATTATTAATGGAGGGGTAAAACATGGTAGTTGGAGATTTCCCAATTGAAACAGATACCATAGTAATCGGAGCAGGTCCTGGTGGATACGTTGCAGCAATTCGTGCa                                                                                    <  2:256720/141‑1 (MQ=255)
                                               cacaTTAAACGTTTATTAAATAATCCAGAATTATTATTAATGGAGGGGTAAAACATGGTAGTTGGAGATTTCCCAATTGAAACAGATACCATAGTAATCGGAGCAGGTCCTGGTGGATACGTTGCAGCAATTCGTGCAGCt                                                                                 <  1:474539/141‑1 (MQ=255)
                                                acaTTAAACGTTTATTAAATAATCCAGAATTATTATTAATGGAGGGGTAAAACATGGTAGTTGGAGGTTTCCCAATTGAAACAGAGACAATAGTAATAGGAGCAGGTCCTGGTGGCTACGATACAGCAATTCgagacgcta                                                                                >  2:587378/1‑133 (MQ=255)
                                                 caTTAAACGTTTATTAAATAATCAAGAATTATTATTAATGGAGGGGTAAAGCATGGTAGTTGGAGATTTCCCAATTGAAACAGATACCATAGTACTCGGAGCAGGTCCTGGTGGATACGTTGCAGCAATTCGTGCAGCTCa                                                                               >  1:80145/1‑141 (MQ=255)
                                                     aaaCGTTTATTAAATAATCCAGAATTACTATTAATGGAGGGGTAAAACATGGTAGTTGGAGATTTCCCAATTGAAACAGATACCATAGTAATCGGAGCAGGTCCTGGTGGATACGTTGCAGCAATTCGTGCAGCTCAATTa                                                                           <  2:450057/141‑1 (MQ=255)
                                                      aacggttctgatagaatCTAGAATTATTATGAATGGAGTGGTTAAACATTGTCGTTGGAGGTGTCGCAATTGACACCTGTACCAGAGTAACCGGAGCAAGGCCTGGTGGATACGGTGCAGCAATTCGTGCAGCTCAATTAg                                                                          <  2:114521/127‑1 (MQ=255)
                                                        cGTTTATTAAATAACCAAGGATAGTGATTAATGGAGGGGTAAAACATGGTAGTTGGAGATTTCCCAATTGAATCAGATACCATAGTAATCGGAGCAGGTCCTGGTGGATAGGTTGAAGCAATTCGGGCAGCTCAATTAGGa                                                                        >  2:356468/1‑141 (MQ=255)
                                                         gTTTATTAAATAATCCAGAATTATTATTAATGGAGGGGTAAAACATGGTAGTTGGAGATTTCCCAATTGAAACAGATACCATAGTAATCGGAGCAGGTCCTGGTGGATACGTTGCAGCAATTCGTGCAGCTCAATTAGGAc                                                                       <  1:742269/141‑1 (MQ=255)
                                                         gTTTATTAAATAATCCAGAATTATTATTAATGGAGGGGTAAAACATGGTAGTTGGAGATTTCCCAATTGAAACAGATACCATAGTAATCGGAGCAGGTCCTGGTGGATACGTTGCAGCAATTCGTGCAGCTCAATTAGGAc                                                                       <  1:229305/141‑1 (MQ=255)
                                                          tttATTAAATAATCCAGAATTATTATTAATGGAGGGGTAAAACATGGTAGTTGGAGATTTCCCAATTGAAACAGATACCATAGTAATCGGAGCAGGTCCTGGTGGATACGTTGCAGCAATTCGTGCAGCTCAATTAGGACa                                                                      <  1:660365/141‑1 (MQ=255)
                                                          tttATTAAATAATCCAGAATTATTATTAATGGAGGGGTAAAACATGGTAGTTGGAGATTTCCCAATTGAAACAGATACCATAGTAATCGGAGCAGGTCCTGGTGGATACGTTGCAGCAATTAGTGCAGGTAAATTAGGACa                                                                      >  1:137801/1‑141 (MQ=255)
                                                           ttattaAATAATCCAGAATTATTATTAATGGAGGGGTAAAACATGGTAGTTGGAGATTTCCCAATTGAAACAGATACCATAGTAATCGGAGCAGGCCCTGGTGGATACGTTGCAGCAATTCGTGCAGCTCAATTAGGACaa                                                                     >  2:547519/1‑141 (MQ=255)
                                                            tattaAATAATCCAGAATTATTATTAATGGAGGGGTAAAACATGGTAGTTGGAGATTTCACAATTGAAACATATACCAGAGTAATCGGAGCAGGTCATGGTGGATACGTTGAGGAAATCCGTGCGGCTCAATTAGGAGaaa                                                                    >  2:57853/1‑141 (MQ=255)
                                                              ttaAATAATCCAGAATTATTATTAATGGAGGGGTAAAACATGGTAGTTGGAGATTTCCCAATTGAAACAGATACCATAGTAATCGGAGCAGGTCCTGGTGGATACGTTGCAGCAATTCGTGCAGCTCAATTAGGACaaaaa                                                                  >  1:717944/1‑141 (MQ=255)
                                                              ttaAATAATCCAGAATTATTATTAATGGAGGGGTAAAACATGGTAGTTGGAGATTTCCCAATTGAAACAGATACCATAGTAATCGGAGCAGGTCCTGGTGGATACGTTGCAGCAATTAGTGGAGCTCAGTTAGGACaaaaa                                                                  >  2:59458/1‑141 (MQ=255)
                                                               tacataatCCAGAATTATTATTAATGGAGGGGTAAAACATGGTAGTTGGACATTACCCAATTGAAACAGATACCATAGTAATCGGAGCAGGTCCTGGTGGATACGTTGCAGTAATTCGTGCAGCTCAATTAGGACAAAAAg                                                                 <  2:191888/138‑1 (MQ=255)
                                                                 aataatCCAGAATTATTATTAATGGAGGGGTAAAACATGGTAGTTGGAGATTTCCCAATTGAAACAGATACCATAGTAATCGGAGCAGGTCCTGGTGGATACGTTGCAGCAATTCGTGCAGCTCAATTAGGACAAAAAGTa                                                               <  1:53218/141‑1 (MQ=255)
                                                                   taatCCAGAATTATTATTAATGGGGGGGTCCACCATGGTTGTTGGAGATTTCCCAATTTAAACACATACCATAGTAATCGGAGCAGGTCCTGGTGGATACGTTGCAGCAATTCGTGCAGCTCAATTAGGACAAAAAGTAAc                                                             <  2:258537/141‑1 (MQ=255)
                                                                   taatCCAGAATTATTATTAATGGAGGGGTAAAACATGGTAGTTGGAGATTTCCCAATTGAAACAGATACCATAGTAATCGGAGCAGGTCCTGGTGGATACGTTGCAGCAATTCGTGTAGCTCAATTAGGACAAAAAGTAAc                                                             <  1:564900/141‑1 (MQ=255)
                                                                   taatCCAGAATTATTATTAATGGAGGGGTAAAACATGGTAGTTGGAGATTTCCCAATTGAAACAGATACCATAGTAATCGGAGCAGGTCCTGGTGGATACGTTGCAGCAATTCGTGCAGCTCAATTAGGACAAAAAGTAAc                                                             <  1:358223/141‑1 (MQ=255)
                                                                   taatCCAGAATTATTATTAATGGAGGGGTAAAACATGGTAGTTGGAGATTTCCCAATTGAAACAGATACCATAGTAATCGGAGCAGGTCCTGGTGGATACGTTGCAGCAATTCGTGCAGCTCAATTAGGACAAAAAGTAAc                                                             <  1:180018/141‑1 (MQ=255)
                                                                       ccAGAATTATGATTAATGGAGGGGTAAAACAGGGTAGTTGGAGAGTGCCCAATTGAAACAGATACCATAGTAATCGGAGCAGGTCCTGGTGGATACGTTGCAGCAATTCGTGCAGCTAAAAAAAAAAAAAAAGTAACAATc                                                         <  2:145967/141‑1 (MQ=255)
                                                                        cAGAATTATTATTAATGGAGGGGTAAAACATGGTAGTTGGAGATTTCCCAATTGAAACAGATACCATAGTAATCGGAGCAGGTCCTGGTGGATACGTTGCAGCAATTCGTGCAGCTCAATTAGGACAAAAAGTAACAATCg                                                        <  1:236907/141‑1 (MQ=255)
                                                                          gAATTATTATTAATGGAGGGGTAAAACATGGTAGTTGGAGATTTCCCAATTGAAACAGATACCATAGTAATCGGAGCAGGTCCTGGTGGATGCGTTGCAGCAATTCGTGCAGCCCAATTAGGACAAAAAGTAACAATCGtt                                                      >  1:6630/1‑141 (MQ=255)
                                                                          gAATTATTATTAATGGAGGGGTAAAACATGGTAGTTGGAGATTTCCCAATTGAAACAGATACCATAGTAATCGGAGCAGGTCCTGGTGGATACGTTGCAGCAATTCGTGCAGCTCAATTAGGACAAAAAGTAACAATCGtt                                                      >  1:78995/1‑141 (MQ=255)
                                                                               attattaATGGAGGGGTAAAACAAGTTATTTGGATATTTCCCAATTGAAACAGATACCATAGTAATCGGAGCAGGTCCTGGTGGATACGTTGCAGCAATTCGTGCAGCTCCATTAGGACAAAAAGTAACAATCGTTGAGaa                                                 >  2:452390/1‑141 (MQ=255)
                                                                                 tattaATGGAGGGGTAAAACATTGTAGTTGGAGATTTCCCAATTGAAACAGATACCATAGTAATCGGAGCAGGTCCTGGTGGATACGTTGCAGCAATTCGTGCAGCTCAATTAGGACAAAAAGTAACAATCGTTgggtagg                                               <  2:200100/141‑7 (MQ=255)
                                                                                 tattaATGGAGGGGTAAAACATGGTAGTTGGAGATTTCCCAATTGAAACAGATACCATAGTAATCGGAGCAGGTCCTGGTGGATACGTTGCAGCAATTCGTGCAGCTCAATTAGGACAAAAAGTAACAATCGTTGAGAAAg                                               <  2:200487/141‑1 (MQ=255)
                                                                                   ttaATGGAGGGGTAAAACATGGTAGTTGGAGATTTCCCAATTGAAACAGATACCATAGTAATCGGAGCAGGTCCTGGTGGATACGTTGCAGCAATTCGTGCAGCTCAATTAGGACAAAAAGTAACAATCGTTGAGAAAGGt                                             <  1:32951/141‑1 (MQ=255)
                                                                                   ttaATGGAGGGGTAAAACATGGTAGTTGGAGATTTCCCAATTGAAACAGATACCATAGTAATCGGAGCAGGTCCTGGTGGATACGTTGCAGCAATTCGTGCAGCTCAATTAGGACAAAAAGTAACAATCGTTGAGAAAGGt                                             <  2:93578/141‑1 (MQ=255)
                                                                                   taaaTGGAGGGGTAAAACATGGTATTTGGAGATTTCCCAATTGAAACAGATACCATAGTAATCGGAGCAGGTCCTGGTGGATACGTTGCAGCAATTCGTGCAGCTCAATTAGGACAAAAAGTAACAATCGTTGAGAAAGGt                                             <  1:626436/139‑1 (MQ=255)
                                                                                          aGGGGTAAAACATGGTAGTTGGAGATTTCCCAATTGAAACAGATACCATAGTAATCGGAGCAGGTCCTGGTGGATACGTTGCAGCAATTCGTGCAGCTCAATTAGGACAAAAAGTAAAAATCGTTGAGAAAGGTAATCTtg                                      >  2:520187/1‑141 (MQ=255)
                                                                                           ggggTAAAACATGGTAGTTGGAGATTTCCCAATTGAAACAGATACCATAGTAATCGGAGCAGGTCCTGGTGGATACGTTGCAGCAATTCGTGCAGCTCAATTAGGACAAAAAGTAACAATCGTTGAGAAAGGTAATCTtgg                                     >  1:261745/1‑141 (MQ=255)
                                                                                                     aTGGTAGTTGGAGATTCCCAAATTGAAACAGATACCATGGTAATCGGAGCAGGTCCTGGTGGATACGTTGCAGCAATTCGTGCAGCTCAATTAGGACAAAAAGTAACAATCGTTGAGAAAGGTAATCTTGGTGGTGTTTGc                           >  1:431692/1‑141 (MQ=255)
                                                                                                      tGGTAGTTGGAGATTTCCCAATTGAAACAGATACCATAGTAATCGGAGCAGGTCCTGGTGGATACGTTGCAGCAATTCGTGCAGCTCAATTAGGACAAAAAGTAACAATCGTTGAGAAAGGTAATCTTGGTGGTGTTTGCt                          <  1:61073/141‑1 (MQ=255)
                                                                                                               gagaTTTCCCAATTGAAACAGATACCATAGTAATCGGAGCAGGTCCTGGTGGATACGTTGCAGCAATTCGTGCAGCTCAATTAGGACAAAAAGTAACAATCGTTGAGAAAGGTAATCTTGGTGGTGTTTGCTTAAACGTAg                 <  1:252735/141‑1 (MQ=255)
                                                                                                               gagaTTTCCCAATTGAAACAGATACCATAGTAATCGGAGCAGGTCCTGGTGGATACGTTGCAGCAATTCGTGCAGCTCAATTAGGACAAAAAGTAACAATCGTTGAGAAAGGTAATCTTGGTGGTGTTTGCTTAAACGTAg                 >  2:644116/1‑141 (MQ=255)
                                                                                                               gagaTTTCCCAATTGAAACAGATACCATAGTAATCGGAGCAGGTCCTGGTGGATACGTTGCAGCAATTCGTGCAGCTCAATTAGGACAAAAAGTAACAATCGTTGAGAAAGGGAATCTTGGTGGTGTTTGCTTAAACGTAg                 >  1:140292/1‑141 (MQ=255)
                                                                                                                agaTTTCCCAATTGAAACAGATACCATAGTAATCGGAGCAGGTCCTGGTGGATACGTTGCAGCAATTCGTGCAGCTCAATTAGGACAAAAAGTAACAATCGTTGAGAAAGGTAATCTGGGTGGTGTTTGCTTAAACGTAgg                >  2:114201/1‑141 (MQ=255)
                                                                                                                    ttCCCAATTGAAACAGATACCATAGTAATCGGAGCAGGTCCTGGTGGATATGTTGCAGCAATTCGTGCAGCTCAATTAGGACAAAAAGTAACAATCGTTGAGAAAGGTAATCTTGGTGGTGTTTGCTTAAACGTAGGATGt            >  1:99558/1‑141 (MQ=255)
                                                                                                                    ttCCCAATTGAAACAGATACCATAGTAATCGGAGCAGGTCCTGGTGGATACGTTGCAGCAATTCGTGCAGCTCAATTAGGACAAAAAGTAACAATCGTTGAGAAAGGTAATCTTGGTGGTGTTTGCTTAAACGTAGGATGt            >  2:754458/1‑141 (MQ=255)
                                                                                                                      cccAATTGAAACAGATACCATGGTAATCGGAGCAGGTCCTGGTGGATACGGTGCAGCAATTCGTGCAGCTAAATTAGGACAAAAAGTAACAATCGTTGAGAAAGGTAATCTTGGTGGTCTTTGCTTAAACGTAGGATGTAt          <  2:565128/141‑1 (MQ=255)
                                                                                                                        cAATTGAAACAGATACCATAGTAATCGGAGCAGGTCCTGGTGGATACGTTGCAGCAATTCGTGCAGCTCAATTAGGACAAAAAGTAACAATCGTTGAGAAAGGTAATCTTGGTGGTGTTTGCTTAAACGTAGGATGTATTc        >  2:246910/1‑141 (MQ=255)
                                                                                                                         atttGAAACAGATACCATAGTAATCGGAGCAGGTCCTGGTGGATACGTTGCAGCAATTCGTGTAGCGCAATTAGGACAAAAAGTAACAAGCGTTGAGAAAGGTAATCTTGGTGGTGTTTGCTTAAACGTAGGATGTATTcc       <  1:619399/139‑1 (MQ=255)
                                                                                                                            tGAAACAGATACCATAGTAATCGGAGCAGGTCCTGGTGGATACGTTGCAGCAATTCGTGCAGCTCAATTAGGACAAAAAGTAACAATCGTTGAGAAAGGTAATCTTGGTGGTGTTTGCTTAAACGTAGGATGTATTCCTTc    <  1:679699/141‑1 (MQ=255)
                                                                                                                              aaaCAGCTACCATAGGAACCGGAGCAGGTCCTGGTGGATACGTTGCAGCAATTCGTGCAGGTCAATTAGGACAAAAAGTAACAATCGTTGAGAAAGGTAATCTTGGTGGTGTTTGCTTATACGTCGGATGTATTCCTTCaa  <  1:398050/141‑1 (MQ=255)
                                                                                                                                        |                                                                                                                                  
TTGACCACAGACAAATTGATGGTGCAACTGGCCAAAATGCAATGAATCACATTAAACGTTTATTAAATAATCCAGAATTATTATTAATGGAGGGGTAAAACATGGTAGTTGGAGATTTCCCAATTGAAACAGATACTATAGTAATCGGAGCAGGTCCTGGTGGATACGTTGCAGCAATTCGTGCAGCTCAATTAGGACAAAAAGTAACAATCGTTGAGAAAGGTAATCTTGGTGGTGTTTGCTTAAACGTAGGATGTATTCCTTCAA  >  CP000730/1105858‑1106124

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑