Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 1,929,861 T→C 66.1% intergenic (‑944/‑224) USA300HOU_1794 ← / → USA300HOU_1796 hypothetical protein/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007301,929,8610TC66.1% 227.9 / 116.2 174intergenic (‑944/‑224)USA300HOU_1794/USA300HOU_1796hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (36/23);  new base C (61/52);  total (99/75)
Fisher's exact test for biased strand distribution p-value = 4.20e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 177 total aligned reads displayed.

ATTAAGTGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTG‑TA‑T‑AA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACATATAGCCGCACCAATAAAAATCCCCTCACTA  >  CP000730/1929721‑1929999
                                                                                                                                             |                                                                                                                                               
aTTAAGGGGAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGgtg‑t                                                                                                                                                 <  2:695065/141‑1 (MQ=255)
    aGTGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCGGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGCTAATTTAGACGCAGAGAGGAGGTG‑TA‑T‑aa                                                                                                                                           >  1:437386/1‑141 (MQ=255)
    aGTGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTG‑TA‑T‑aa                                                                                                                                           >  1:156165/1‑141 (MQ=255)
     gtgtAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGCG‑TA‑T‑CA‑g                                                                                                                                         >  2:100176/1‑141 (MQ=255)
     gtgtAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACCCAGAGCGGCGGTG‑TA‑A‑AA‑g                                                                                                                                         >  2:205334/1‑141 (MQ=255)
            cGATTCAATCTATCGGCTAAAATCAAATAACTTATCAGAAGCAACTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTG‑TA‑T‑AA‑G‑G‑TGATGc                                                                                                                                >  1:423686/1‑141 (MQ=255)
            cGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTG‑TA‑T‑AA‑G‑G‑TGATGc                                                                                                                                >  1:386619/1‑141 (MQ=255)
            cGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCATAAATGCAGACGCAGAGAGGAGGGG‑TA‑C‑AA‑G‑G‑TGATGc                                                                                                                                >  1:478470/1‑141 (MQ=255)
                  aaTCTTTCAGCTGAGATCATATCACTGATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTG‑TA‑T‑AA‑G‑G‑TGATGCTTAttt                                                                                                                          <  2:638542/141‑1 (MQ=255)
                     cTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTG‑TA‑T‑AA‑G‑G‑TGATGCTTATTTTCg                                                                                                                       <  1:534176/141‑1 (MQ=255)
                                      ataACTTATCAGAAGCAAGAGACTGTGCTTGTATATTTGCCGATGATATAATAGCAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTG‑TA‑T‑AA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCa                                                                                                      >  1:503233/1‑141 (MQ=255)
                                         aCTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCAATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTG‑TA‑T‑AA‑G‑G‑TGATGCTTCTGTTCGTTCACGTCATAGCACCAGtc                                                                                                   >  1:377276/1‑141 (MQ=255)
                                          cTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTG‑TA‑T‑AA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGtca                                                                                                  >  2:643536/1‑141 (MQ=255)
                                                 atcggaagtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATT‑AC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGc                                                                                          >  2:561683/15‑141 (MQ=255)
                                                 atcggaagtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATT‑AC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGc                                                                                          >  1:307206/15‑141 (MQ=255)
                                                 atcggaagtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATT‑AC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGc                                                                                          <  1:117662/127‑1 (MQ=255)
                                                  tcggaagtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATT‑AC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCt                                                                                         >  1:189291/14‑141 (MQ=255)
                                                   cggaagtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCGATT‑‑GACGAAGAGAGGAGGTG‑CA‑T‑TACT‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCtg                                                                                        >  1:456350/13‑141 (MQ=255)
                                                    ggaagtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATT‑AC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCtgt                                                                                       <  1:619722/130‑1 (MQ=255)
                                                    ggaagtagatacgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CA‑T‑TACT‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCtgt                                                                                       <  2:505844/129‑1 (MQ=255)
                                                     gaagttggtatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGCGGAGTTG‑CATT‑AC‑T‑T‑TGCTAATCATTTTCGTTCACATCAGAGCACCAGTCATCAGTGGCtgtg                                                                                      <  1:255424/131‑1 (MQ=255)
                                                     gaagtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATT‑AC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCtgtg                                                                                      >  2:606465/11‑141 (MQ=255)
                                                      aattagatatgCCTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CA‑T‑TA‑CTT‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGc                                                                                     <  2:555817/132‑1 (MQ=255)
                                                      aaGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTG‑TA‑T‑AA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCtgtg                                                                                      <  2:446337/141‑1 (MQ=255)
                                                       actagatacgCTTGTATATTCACAGACGGTATAATAATAGAGTTGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CA‑T‑TA‑C‑TTTGCTAATCATTTTCGTTCCCATCATAGCACCAGTCATCAGTGGCTGTGct                                                                                    <  1:192797/132‑2 (MQ=255)
                                                        gtagatatgCCTGTATACTCACAGATGGTACAATAATAGCGTCGCCCACCTCGCGTCCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATT‑AC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGctg                                                                                   <  1:21780/134‑3 (MQ=255)
                                                        gTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTG‑TA‑T‑AA‑G‑G‑TGAGGCGGGTTTTCGTTCACATCATGGCACCAGTCATCAGTGGCTGTGcc                                                                                    >  1:524609/1‑141 (MQ=255)
                                                         tagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATT‑AC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGctgt                                                                                  <  1:316784/135‑4 (MQ=255)
                                                         tagatatgCTTGTATATTCACAGATGGTATAAGAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATT‑AC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGctgt                                                                                  <  2:464364/135‑4 (MQ=255)
                                                          agatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATT‑AC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGctgtt                                                                                 >  1:550046/6‑137 (MQ=255)
                                                           gatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATT‑AC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGctgttg                                                                                >  1:24742/5‑136 (MQ=255)
                                                           gatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CA‑T‑TA‑CTT‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGctgttg                                                                                >  1:646252/5‑136 (MQ=255)
                                                           ccggtgCTTGGAGATTTGCCGATGATATAATAGTAGAGTAGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGCG‑TA‑T‑AA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCCTCAGTGGCTGTGCCAtt                                                                                 <  2:209532/138‑1 (MQ=255)
                                                             tgtgCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTG‑TA‑T‑AA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGc                                                                               <  1:60674/141‑1 (MQ=255)
                                                             tatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATT‑AC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGAGCTGTTGCg                                                                              <  1:567487/139‑1 (MQ=255)
                                                             tatgCTTGTATATGCACAGATGGTAAAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATT‑AC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCg                                                                              <  1:698596/139‑1 (MQ=255)
                                                              atgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATT‑AC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGt                                                                             <  1:105831/140‑1 (MQ=255)
                                                                   gttattTTGGCCGATGATATAATAGTAGAGTCGCTTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTG‑TA‑T‑AA‑G‑T‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGttttt                                                                         <  2:395127/139‑1 (MQ=255)
                                                                    gTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATT‑AC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGtattata                                                                       <  1:538346/141‑7 (MQ=255)
                                                                     tataTTCACAGATGGTATAATAATAGATTCGCCTATCTCTCAGGCGTCAATT‑‑GACCAAGAGAGGAGGTG‑CA‑T‑TA‑CTT‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGtattatac                                                                      <  1:365937/141‑8 (MQ=255)
                                                                        aattaccaatgatATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTG‑TA‑T‑AA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCtt                                                                    <  1:333251/133‑1 (MQ=255)
                                                                         tttGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCATCAATTTAGACGCAGAGACACGGTG‑TA‑T‑AA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCACCAGTGACTGTTCCCTTGCGtttttttactt                                                                   >  2:174548/1‑137 (MQ=255)
                                                                          tcacagatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATT‑AC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTAtt                                                                 >  1:736519/6‑141 (MQ=255)
                                                                            acagatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATT‑AC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTgg                                                               >  1:687411/4‑141 (MQ=255)
                                                                            acagatgggataataataGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CA‑T‑TA‑C‑TTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTgg                                                               <  1:71719/132‑1 (MQ=255)
                                                                              agttggtataATAATAGAGTCGCCTAGCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATT‑AC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCt                                                             <  1:639072/135‑1 (MQ=255)
                                                                               gatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATT‑AC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCtt                                                            >  2:489279/1‑140 (MQ=255)
                                                                                 tggtataATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CC‑T‑TACT‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAg                                                          >  1:297551/4‑141 (MQ=255)
                                                                                 tggtataATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATT‑AC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAg                                                          >  1:324897/4‑141 (MQ=255)
                                                                                  ggtataATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATT‑AC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGt                                                         >  1:409270/3‑141 (MQ=255)
                                                                                  ggtataATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATT‑AC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGt                                                         >  1:582952/3‑141 (MQ=255)
                                                                                    tataATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CA‑T‑TACT‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTaa                                                       >  2:579148/1‑140 (MQ=255)
                                                                                    tataATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CA‑T‑TACT‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTaa                                                       >  2:668643/1‑140 (MQ=255)
                                                                                     ataataataGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATT‑AC‑T‑T‑TGCTACTCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTaaa                                                      <  2:576585/141‑3 (MQ=255)
                                                                                        ataataGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATT‑AC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGc                                                   <  2:158035/141‑1 (MQ=255)
                                                                                        ataataGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATT‑AC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGc                                                   <  1:293933/141‑1 (MQ=255)
                                                                                        ataGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTG‑TA‑T‑AA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACg                                                    <  1:247138/141‑1 (MQ=255)
                                                                                        agtatagAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATT‑AC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGc                                                   <  1:632896/137‑1 (MQ=255)
                                                                                          aatagATTCGCCTATCTCTCAGGCGTCAA‑‑TGGACTAAGAGAGGAGGTG‑CATT‑AC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaa                                                 <  2:770001/139‑1 (MQ=255)
                                                                                          aatagAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CA‑T‑TACT‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaa                                                 <  2:749139/139‑1 (MQ=255)
                                                                                            tagAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CA‑T‑TACT‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaaca                                               >  2:758991/1‑139 (MQ=255)
                                                                                            tagAGTCGCCTATCCCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATT‑AC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaaca                                               <  2:264399/141‑3 (MQ=255)
                                                                                            atgagTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATT‑AC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaaca                                               <  1:544764/139‑3 (MQ=255)
                                                                                             agagTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTG‑TA‑T‑AA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATa                                               <  2:478470/141‑1 (MQ=255)
                                                                                             agagTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATT‑AC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTACACGCaacaa                                              >  2:416835/1‑138 (MQ=255)
                                                                                             agagTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATT‑AC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaa                                              <  1:244552/141‑4 (MQ=255)
                                                                                              gagTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATT‑AC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCGGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaa                                             >  1:59597/1‑137 (MQ=255)
                                                                                              gagTCGCCTATCCCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTG‑TA‑T‑AA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATAc                                              <  2:41598/141‑1 (MQ=255)
                                                                                               agTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATT‑AC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaat                                            >  2:72282/1‑136 (MQ=255)
                                                                                                gTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTG‑TA‑T‑AA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACaa                                            <  2:32723/141‑1 (MQ=255)
                                                                                                gTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTG‑TA‑T‑AA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACaa                                            <  2:698408/141‑1 (MQ=255)
                                                                                                gTCGCCTATCTCTCAGGCGTCAATT‑‑GACCAAGCGAGGAGGTG‑CATT‑AC‑T‑T‑TGCTAATCATTTCCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaata                                           <  1:315589/141‑7 (MQ=255)
                                                                                                gTCGCCGATCTCTCAGGCGTCAATT‑‑GAAGAAGAGAGGAGGTGCGT‑T‑AC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaata                                           >  2:196975/1‑135 (MQ=255)
                                                                                                 tCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATT‑AC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataa                                          >  2:113229/1‑134 (MQ=255)
                                                                                                   gCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATT‑AC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataatt                                        >  1:287339/1‑132 (MQ=255)
                                                                                                   gCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATT‑AC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataatt                                        <  2:231325/141‑10 (MQ=255)
                                                                                                   gCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATT‑AC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataatt                                        <  2:154858/141‑10 (MQ=255)
                                                                                                   gCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CA‑T‑TACT‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataatt                                        <  2:155011/141‑10 (MQ=255)
                                                                                                   gCCTATCTCTAAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATT‑AC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataatt                                        <  2:284388/141‑10 (MQ=255)
                                                                                                    ccTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CC‑TCAC‑G‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCACCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataataa                                       >  1:730734/1‑131 (MQ=255)
                                                                                                    ccTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATT‑AC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataatta                                       >  1:667696/1‑131 (MQ=255)
                                                                                                    ccTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATT‑AC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataatta                                       >  1:491941/1‑131 (MQ=255)
                                                                                                    ccTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CA‑T‑TACT‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataatta                                       >  2:616714/1‑131 (MQ=255)
                                                                                                    ccTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CA‑T‑TACT‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataatta                                       >  1:457055/1‑131 (MQ=255)
                                                                                                     cTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATT‑AC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataattaa                                      >  2:85138/1‑130 (MQ=255)
                                                                                                     cTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATT‑AC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataattaa                                      >  1:744668/1‑130 (MQ=255)
                                                                                                      tATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATT‑AC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataattaat                                     <  2:613140/141‑13 (MQ=255)
                                                                                                      tATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATT‑AC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataattaaa                                     >  1:694320/1‑129 (MQ=255)
                                                                                                       aTCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATT‑AC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataattaata                                    >  1:325699/1‑128 (MQ=255)
                                                                                                       aTCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATT‑AC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataagtaata                                    >  1:171442/1‑128 (MQ=255)
                                                                                                       aTCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CA‑T‑TA‑C‑TTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataattaata                                    >  2:666719/1‑128 (MQ=255)
                                                                                                            tcAGGCGTCAATTTAGACGCAGAGAGGAGGTG‑TA‑T‑AA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACat                                >  1:608235/1‑141 (MQ=255)
                                                                                                            tcAGGCGTCAATTTAGACGCAGAGAGGAGGAG‑TA‑T‑AA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTAGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAACATGGGTGGCat                                >  2:256390/1‑141 (MQ=255)
                                                                                                              aGGCGTCAATTTAGACGCAGAGAGGAGGGG‑TA‑T‑AA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGGGGCatat                              >  2:587540/1‑141 (MQ=255)
                                                                                                                            aCGCAGAGAGGAGGTG‑TA‑T‑AA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTGTTATTCGGCCTGCCTAAATCGGCGCAATACAAAATAGGTGACATATAGCCGCCCCAAGaa                >  2:316106/1‑141 (MQ=255)
                                                                                                                              gctgagcgGAGGTG‑TA‑T‑AA‑G‑G‑GGATGCTTATTTTCGTTCCCATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACATATAGCCGCACCAATaaaa              <  2:379884/138‑1 (MQ=255)
                                                                                                                               cAGAGCGGAACTG‑TG‑T‑AA‑G‑C‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACATATAGCCGCACCAATaaaaa             <  1:756816/141‑1 (MQ=255)
                                                                                                                                      gaggTG‑TA‑T‑AA‑G‑G‑TGATGATAGGGTGCTGTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACATATAGCCGCACCAATAAAAATCCCCTc      >  2:748512/1‑141 (MQ=255)
                                                                                                                                         gtgtta‑t‑aA‑G‑G‑TGATGCTTATTTTCTGTCACATCATAGCACCATTCATCAGTGGCTGCGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACATATAGCCGTACCAATAAAAATCCCCTAAc    <  2:299320/137‑1 (MQ=255)
                                                                                                                                          tg‑tA‑T‑AA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACATATAGCCGCACCAATAAAAATCCCCTCACTa  <  2:246895/141‑1 (MQ=255)
                                                                                                                                             |                                                                                                                                               
ATTAAGTGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTG‑TA‑T‑AA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACATATAGCCGCACCAATAAAAATCCCCTCACTA  >  CP000730/1929721‑1929999

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑