BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP000730	35,913	C→G	21.3%	S213S (TCG→TCC)	USA300HOU_0026 ←	IS431mec transposase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	35,913	0	C	G	21.3%	459.2 / 182.9	328	S213S (TCG→TCC)	USA300HOU_0026	IS431mec transposase
Reads supporting (aligned to +/- strand): ref base C (101/154); new base G (35/35); total (137/191)
Fisher's exact test for biased strand distribution p-value = 1.33e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01

Only 100 of 333 total aligned reads displayed.

CTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTC  >  CP000730/35773‑36053

         gagCTAAAATAAAATTGTGATCTAATAAAAATAGAAGCTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCACGGCGAGAATCCg                                                                                                                                     >  2:126760/1‑141 (MQ=255)

          agCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGt                                                                                                                                    >  2:624081/1‑141 (MQ=255)

          agCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGt                                                                                                                                    >  1:418726/1‑141 (MQ=255)

            gtaaaataaaaTTGTGATCTAATAAAAATAGAAGGTTCTGTTTCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAg                                                                                                                                  <  2:36323/140‑1 (MQ=255)

            ataaaaaaaaaTTGTGATCTAATAAAAATAGAAGGCTCTGTTGCAAAGTAAAAAAAGATAGCTAACCACTAATTTATCATGGCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAg                                                                                                                                  <  2:532436/140‑1 (MQ=255)

                 acaaaagagtattttaataaAAAT‑GATGGTTCTGTTGCAAAGT‑AAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGaa                                                                                                                           >  1:440208/15‑141 (MQ=11)

                      agagtaattcaataaAAAT‑GATGGTTCTGTTGCAAAGT‑AAAAAATTTAGATTACCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGAc                                                                                                                      <  1:682058/131‑1 (MQ=11)

                       gagtattttaataaAAAT‑GATGGTTCTGTTGCAAAGT‑AAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGAcc                                                                                                                     >  2:402488/9‑141 (MQ=11)

                        agtattttaataaAAAT‑GATGGTTCTGTTGCAAAGT‑AAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCt                                                                                                                    <  2:672182/134‑1 (MQ=11)

                         gtattttaataaAAAT‑GATGGTTCTGTTGCAAAGT‑AAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTg                                                                                                                   <  2:72156/135‑1 (MQ=11)

                          ggATCGAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCt                                                                                                                    <  1:232169/140‑1 (MQ=255)

                            ttttaataaAAAT‑GATGGTTCTGTTGCAAAGT‑AAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCgg                                                                                                                >  2:524611/4‑141 (MQ=11)

                            ttttaataaAAAT‑GATGGTTCTGTTGCAAAGT‑AAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCgg                                                                                                                <  2:36853/138‑1 (MQ=11)

                            aTCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGc                                                                                                                  <  1:378033/141‑1 (MQ=255)

                             tttaataaAAAT‑GATTGTTCTGTTGCAAAGT‑AAAAAATATACCCAATCCCAAATACTTCATGTAAGTCTTTGTTTAACTTGCTAGCATTATGCTAATTTAGTGGCATGGCGAAAATCCGTAGACCTGAAGAGACCTGCGGt                                                                                                               <  2:193526/139‑1 (MQ=2)

                             ttgaataaAAAT‑GATGCTTCTGTTGCAAAGT‑AAAAAATATAGCTAATCACTAATATAGCATGTCAGTGTTCGTTTAACTTGCTAGCATGTTGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGt                                                                                                               <  1:245161/138‑1 (MQ=11)

                              ttaataaAAAT‑GATGGTTCTGTTGCAAAGT‑AAAAAATATAGCTAAGCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGtt                                                                                                              <  2:682348/140‑1 (MQ=11)

                              cTAATAACAATAGAAGGTTCTGTTGCAAAGTAAAAAGATATAGCTAACCACTAATTTATCAGTGCAGTGGTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGAgg                                                                                                                >  2:261106/1‑141 (MQ=14)

                              cTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTACTTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCgg                                                                                                                >  1:287213/1‑141 (MQ=255)

                               taataaAAAT‑GATGGTTCTGTTGCAAAGT‑AAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTc                                                                                                             <  1:471129/141‑1 (MQ=11)

                               taataaAAAT‑GATGGTTCTGTTGCAAAGT‑AAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTc                                                                                                             <  1:587593/141‑1 (MQ=11)

                                  taaAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGCAAATCCGTAGATCTGAAGAGACCTGCGGTTCt                                                                                                            >  1:108364/1‑141 (MQ=38)

                                                           atataaaaaTATAGGCAACCACTATCTTATCATGTATGTGTCCGCTTAACTCTCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACATTc                                                                                   <  2:142673/137‑1 (MQ=1)

                                                                                   atatATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGc                                                           <  1:25148/138‑1 (MQ=0)

                                                                                              cAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATAc                                                >  1:752998/1‑141 (MQ=11)

                                                                                               aGTGTTCGTTTAACTTGGTAGCATGATGCCAATTTCGTGGCATGCGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACt                                               <  1:292130/141‑1 (MQ=11)

                                                                                               aGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACt                                               <  1:659847/141‑1 (MQ=14)

                                                                                               aGTGTTCGTTTAACTAGCTGGCATGATGCTAATTTCGTGGCACGGCGAAAATCCGTAGATCTGATGAGACCTGCCGTTCTTTTTATATAAAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACt                                               >  1:577575/1‑141 (MQ=11)

                                                                                                gtgtTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCGTGGGGAAAATCCGTGGAGCTGAAGAGACCTACGGTTCTTTTTATATCGAGCGTAAATACCTTCAATACCTTTTACAGTATTCTTTGCCGTATTGATACtt                                              >  1:1307/1‑141 (MQ=11)

                                                                                                gtgtTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTCCAGTACTCTTTGCTGTATTGATACtt                                              <  1:569655/141‑1 (MQ=11)

                                                                                                 tgtTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTATAGTATTATTTGCTGGATTGATACttt                                             >  2:740102/1‑141 (MQ=11)

                                                                                                 tgtTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGTTACttt                                             >  2:398173/1‑141 (MQ=12)

                                                                                                 tgtTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACttt                                             <  2:87287/141‑1 (MQ=17)

                                                                                                 tgtTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACttt                                             <  1:731042/141‑1 (MQ=17)

                                                                                                 ggtTCGTTTAACTTGCTAGCATGATGCTAATTCCGTGGCATGGGGAAAATCCGTTGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGAGTAAATACAGTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACttt                                             <  2:570734/140‑1 (MQ=11)

                                                                                                  gtTCGGTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTCGTTCTGAAGAAACCTGCCGTTCTTTTTATATAGAGCGTAGATAGAGCCAATACATTTTAAAGTATTCTTTGCTGTATTGATACTTTg                                            >  2:286966/1‑141 (MQ=11)

                                                                                                   ttCGTTTAACTTGCTAGCATGATGCTCATTTCGTGGCCTGGCGAAAAGCCGTAGATCTGAAGACACGTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTGACAGTGTTCTTTGCTGTATTGTTACTTGGa                                           >  2:491449/1‑141 (MQ=11)

                                                                                                   ttCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGa                                           >  2:583692/1‑141 (MQ=12)

                                                                                                   ttCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATCTAGACCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGa                                           <  1:145930/141‑1 (MQ=12)

                                                                                                    tCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGCTCTGGATACACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGAt                                          <  1:761209/141‑1 (MQ=11)

                                                                                                     cgtttAACTTGCTAGCATGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTACCGTATTGATGCTTTGATa                                         >  1:270363/4‑141 (MQ=11)

                                                                                                      gtttAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATAc                                        <  2:434122/139‑1 (MQ=17)

                                                                                                      gtttAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATAc                                        <  2:663480/139‑1 (MQ=17)

                                                                                                       tttAACTTGCTAGCATGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGGCCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATatc                                       <  2:554276/140‑3 (MQ=11)

                                                                                                       gttAATTTGCTAGTCTGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGACCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATatc                                       <  2:467970/140‑3 (MQ=11)

                                                                                                       cTTAACTTGCTAGCATGTTGGTAAGTTCTGTGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATAcc                                       <  2:540537/141‑1 (MQ=11)

                                                                                                       cTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATAcc                                       >  1:451101/1‑141 (MQ=17)

                                                                                                        ttAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCt                                      <  2:366436/141‑1 (MQ=17)

                                                                                                         tAACTTGCTAGCATGATGCTAATTTCGTGGCATGTCGAAAATCCGTAGCTCTGCAGAGACCTGCGGTTCTGTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCtt                                     >  1:767429/1‑141 (MQ=11)

                                                                                                          aaCTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTg                                    <  1:95436/141‑1 (MQ=17)

                                                                                                          aaCCTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTg                                    <  1:483235/141‑1 (MQ=17)

                                                                                                           aCTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGt                                   <  2:642198/141‑1 (MQ=17)

                                                                                                             ttGCTAGCATGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGtct                                 <  1:88494/141‑1 (MQ=255)

                                                                                                             ttGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTATATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGtct                                 <  2:505784/141‑1 (MQ=17)

                                                                                                             ttGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGtct                                 <  1:698605/141‑1 (MQ=17)

                                                                                                              tGCTAGCATGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGtctt                                <  1:720763/141‑1 (MQ=11)

                                                                                                              tGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGtctt                                >  2:478493/1‑141 (MQ=17)

                                                                                                               gCTAGCATGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTCTATAGAGCGTAAATACATTCAATACATTTTAAAGTATTCTTTGCCGTATTGAACCTTTGATATCtttttttt                               >  1:622514/1‑141 (MQ=11)

                                                                                                               gCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACGTTGtcttt                               >  2:220892/1‑141 (MQ=17)

                                                                                                               gCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGtcttt                               >  2:319416/1‑141 (MQ=17)

                                                                                                                ctctcATGATGCTAATTTCGTTGCATGGCGAAAATCCGTAGATCTGAAGAGATCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTATAGTATTCTTAGTAGTGGGGATACGTTGATACCTTGtctttc                              <  1:374305/137‑1 (MQ=11)

                                                                                                                cTAGCATGATGCTAATTTCGTGGCATGGGGAAAAGCCGTAGAACTCAAGAGACGTACGGTTCTTTTGATATAGAGCGTAAATGCATGCAATACCTTTTAAAGCATTCTTTGCCGCAGTGATACTGTGATATCTTgcgttgt                              >  2:113780/1‑135 (MQ=11)

                                                                                                                 tggCAGGATGGTAATTTGGGGGCATTGCGAATATCCTTAGATCTGAAGTGACCTGTGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTGTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGtctttct                             <  2:335453/139‑1 (MQ=11)

                                                                                                                 tAGCATGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGtctttct                             <  1:394277/141‑1 (MQ=11)

                                                                                                                 tAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGtctttct                             <  1:306918/141‑1 (MQ=17)

                                                                                                                 tAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGtctttct                             <  1:402488/141‑1 (MQ=17)

                                                                                                                  aGCATGATGCTAATTTCGTGTCATGGCGAAAATCCGTAGATCTGTAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAGTACCTTTTAAAGTAGTCTTGGCTGTATTGATACTTTGATACCTTGtctttctt                            >  2:423498/1‑141 (MQ=11)

                                                                                                                  aGCATGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGtctttctt                            <  1:34546/141‑1 (MQ=255)

                                                                                                                  aGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGtctttctt                            >  1:597283/1‑141 (MQ=17)

                                                                                                                  aGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTCGtctttctt                            >  2:176063/1‑141 (MQ=17)

                                                                                                                   gCATGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATTTTGtctttcttt                           >  1:544879/1‑140 (MQ=11)

                                                                                                                   gCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTACAAATTTCTTTGCTGTATTGATACTTTGATACCTTGtctttcttt                           >  1:332138/1‑140 (MQ=11)

                                                                                                                   gCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGGTACCTTGTCTTTCTTa                           >  1:182602/1‑141 (MQ=17)

                                                                                                                   gCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGATCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTa                           >  2:275906/1‑141 (MQ=17)

                                                                                                                    cATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTAc                          >  2:238339/1‑141 (MQ=18)

                                                                                                                     ttgatgCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACt                         <  1:669154/140‑1 (MQ=21)

                                                                                                                     atgatgTTAATTTCGGGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGCCAGTTTGATACCTTGTCTTTCTTACt                         <  2:355521/141‑1 (MQ=11)

                                                                                                                     atgatgCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATCTCTTGTCTTTCTTACt                         >  2:254232/1‑141 (MQ=11)

                                                                                                                     atgatgCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATTCTTTTTTCTCTTGTCTTTCTTACt                         >  2:154612/1‑141 (MQ=11)

                                                                                                                        atgCTAATTTCGTGGCATGGGGAAAAGCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACATTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTa                      <  2:342440/141‑1 (MQ=11)

                                                                                                                          gCTAATTTCGGGGCACGGGGAAAATACGCAGATCGGAAGAATTATGTCTTACGCTTGATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAat                    <  1:364094/141‑1 (MQ=2)

                                                                                                                            caaTTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAatat                  <  1:165546/140‑1 (MQ=11)

                                                                                                                             aaTTGCGTTGCATGGCGAAAGTCCGTAGCTCTGAAGAGACCTGGGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATg                 <  2:595236/141‑1 (MQ=11)

                                                                                                                                ttCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACg              <  1:259070/141‑1 (MQ=255)

                                                                                                                                ttCGTGGCATGGCGAAAATCCGTATATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTTATACCTTGTCTCTCTTACTTTAATATGCCg              >  1:60573/1‑141 (MQ=11)

                                                                                                                                ttCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACg              >  1:340369/1‑141 (MQ=25)

                                                                                                                                 tCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACgg             <  1:551759/141‑1 (MQ=38)

                                                                                                                                  cGTGGCATGGCGAAAATCCGTAGATCCGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGt            <  1:311089/141‑1 (MQ=255)

                                                                                                                                   gTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTg           <  1:642725/141‑1 (MQ=255)

                                                                                                                                   gTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTGGTCTTTCTTACTTTAATATGACGGTg           >  1:638971/1‑141 (MQ=255)

                                                                                                                                    gggCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGa          <  1:100253/140‑1 (MQ=255)

                                                                                                                                     ggCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGAt         >  1:215863/1‑141 (MQ=37)

                                                                                                                                     ggCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGAt         <  1:776865/141‑1 (MQ=255)

                                                                                                                                      gCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACGTTAATATGACGGTGAtt        >  1:531642/1‑140 (MQ=11)

                                                                                                                                        aTGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCtt      <  2:454604/141‑1 (MQ=255)

                                                                                                                                        aTGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCtt      <  1:649167/141‑1 (MQ=14)

                                                                                                                                         ttgCGAAAATACGCCGATCTGAAGAGCCCCACGGTTCTTTTTATATAGAGCGTAAATACACTCCATACCTTTTAAAGTATTCTTTGCTGTATTGCTACTTTGATACCTTGTCTTTCTTACTTTAATATCACGGTCATTTTg     >  2:73056/3‑141 (MQ=11)

                                                                                                                                          ggCGAAAATTCGGAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACGTTAATATGACGGTGATCTTGc    <  2:30317/141‑1 (MQ=255)

                                                                                                                                           gCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCt   <  1:514245/141‑1 (MQ=255)

                                                                                                                                           gCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCt   <  1:617291/141‑1 (MQ=255)

CTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTC  >  CP000730/35773‑36053

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`