Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 35,940 G→A 24.5% R204R (CGC→CGT USA300HOU_0026 ← IS431mec transposase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP00073035,9400GA24.5% 597.8 / 244.6 448R204R (CGC→CGTUSA300HOU_0026IS431mec transposase
Reads supporting (aligned to +/- strand):  ref base G (138/198);  new base A (57/53);  total (196/252)
Fisher's exact test for biased strand distribution p-value = 5.95e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.54e-01

Only 100 of 456 total aligned reads displayed.

ATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGT  >  CP000730/35801‑36080
                                                                                                                                           |                                                                                                                                             
ttttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCgg                                                                                                                                            <  2:36853/138‑1 (MQ=11)
aTCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGc                                                                                                                                              <  1:378033/141‑1 (MQ=255)
      taaAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGCAAATCCGTAGATCTGAAGAGACCTGCGGTTCt                                                                                                                                        >  1:108364/1‑141 (MQ=38)
          aaTAGAAGGTTATGATGCAAAGAACCAAAATATAGCTAACTACTAATTTATCATGTCAGGGTTCGCTTAATTTGCGAGCATGATTCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCttttt                                                                                                                                    >  2:534753/1‑141 (MQ=11)
             aGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTtata                                                                                                                                 >  1:687715/1‑141 (MQ=32)
                       ggtgaaaaGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCGGCGGTTCTTTTTATATAGAGCGTaa                                                                                                                       <  2:57164/136‑1 (MQ=0)
                               agtaacaaaTAAAGCTAATCACTAAGATATCATGTCAGTGTTCGTTTAACTGGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACactc                                                                                                               <  1:77199/135‑4 (MQ=1)
                                                            tcttgtgAGTGTTCGTTTAATTTCCTAGCATGATGCTAATTTCGTGGCCTGGCGAAAATCCGTAGATCTGAAGAGACTTGCGGTTCTTTTTATACAGCGCGTGAAGACACTCAATACCTTTCAAAGTATTCTTTGCTGTAt                                                                                  <  2:364006/138‑1 (MQ=1)
                                                                  cAGTGTTCGTCTAACTTGCTAGCATGATGCTAATTTCGTGACATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTGTTATATAGAGCGTAAATAAATTCAATACCTTTTAAAGTATTATTTGCCGAATTGATAc                                                                            >  2:108195/1‑141 (MQ=11)
                                                                   aGTGTTCGTTTAACTAGCTGGCATGATGCTAATTTCGTGGCACGGCGAAAATCCGTAGATCTGATGAGACCTGCCGTTCTTTTTATATAAAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACt                                                                           >  1:577575/1‑141 (MQ=11)
                                                                   aGTGGTCGTTTAGCTTGCTAGCATGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATAGATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACt                                                                           >  1:546951/1‑141 (MQ=11)
                                                                    gtgtTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACtt                                                                          >  2:507829/1‑141 (MQ=255)
                                                                    gtgtTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGAGAAGATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACtt                                                                          <  1:21933/141‑1 (MQ=255)
                                                                     tgtTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACttt                                                                         <  2:400798/141‑1 (MQ=255)
                                                                     tgtTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTATAGTATTATTTGCTGGATTGATACttt                                                                         >  2:740102/1‑141 (MQ=11)
                                                                     tgtTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACttt                                                                         <  2:87287/141‑1 (MQ=17)
                                                                      gtTCGGTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTCGTTCTGAAGAAACCTGCCGTTCTTTTTATATAGAGCGTAGATAGAGCCAATACATTTTAAAGTATTCTTTGCTGTATTGATACTTTg                                                                        >  2:286966/1‑141 (MQ=11)
                                                                      gtTCGCTTCACTTGCTAGCATGATGCTAATTTCGTGGCACGGCGAAAATCCGTAGATCTGCAGAGACCTGCGGTTCTTTTTATATAGACCGTAAATACACTCAATACCTTGTAAAGTATTCTTTGCTGTATTAGTACTTTg                                                                        >  2:486094/1‑141 (MQ=11)
                                                                       ttCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGa                                                                       >  1:144662/1‑141 (MQ=17)
                                                                       ttCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGa                                                                       >  1:170783/1‑141 (MQ=17)
                                                                        tctcTTAACTTGCTAGCATGATGCTAATTTCGTGGCATTGCGAAGATCCGTAGATCTGAAGAGACCTGCTGTTCTTTTTATATAGAGCGTAAATAAAATCAATACATTTTAAAGTATTATTTGCTGTATTGATACTTTTAt                                                                      >  2:211967/4‑141 (MQ=11)
                                                                         cGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATa                                                                     >  1:39537/1‑141 (MQ=32)
                                                                          gtttAACTTGCTAGCATGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATat                                                                    >  1:492431/3‑140 (MQ=11)
                                                                          gCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATAc                                                                    <  1:368981/141‑1 (MQ=17)
                                                                           tttAACTTGCGAGCATGACGCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATatc                                                                   <  2:555603/140‑3 (MQ=11)
                                                                           gttAATTTGCTAGTCTGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGACCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATatc                                                                   <  2:467970/140‑3 (MQ=11)
                                                                            ttAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCt                                                                  <  1:8548/141‑1 (MQ=17)
                                                                             tAACTTGCTATCATGATGCTAATTGCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCtt                                                                 <  1:24598/141‑1 (MQ=12)
                                                                              aaCTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACAAACTATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTg                                                                <  1:338802/141‑1 (MQ=12)
                                                                              aaCTTGCTAGCATGATGCCAATTTCGTGGGATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTATTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACGTTg                                                                <  2:244913/141‑1 (MQ=11)
                                                                               aCTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGt                                                               <  2:642198/141‑1 (MQ=17)
                                                                               aCTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGt                                                               <  1:1495/141‑1 (MQ=17)
                                                                                cTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAGAGTATTCTTTGCTGTATTGATACTTTGATACCTTGtc                                                              >  2:223914/1‑141 (MQ=17)
                                                                                    cTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGtctttt                                                          >  1:724083/1‑140 (MQ=17)
                                                                                     tAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGtctttct                                                         >  1:449787/1‑141 (MQ=32)
                                                                                     tAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGtctttct                                                         <  1:306918/141‑1 (MQ=17)
                                                                                     aaGCAGGAGGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGtctttct                                                         <  2:115687/140‑1 (MQ=255)
                                                                                      aGCATGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGtctttctt                                                       <  1:34546/141‑1 (MQ=255)
                                                                                       gCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTACAAATTTCTTTGCTGTATTGATACTTTGATACCTTGtctttcttt                                                      >  1:332138/1‑140 (MQ=11)
                                                                                         atgatgCTAATTTCGTGGCATGGGCAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACt                                                    <  1:256516/141‑1 (MQ=11)
                                                                                          tgatgCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTGTATATAGTGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACtt                                                   <  2:144756/141‑1 (MQ=11)
                                                                                          tgatgCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACtt                                                   <  1:427471/141‑1 (MQ=25)
                                                                                          tgatgCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACtt                                                   >  2:67500/1‑141 (MQ=21)
                                                                                            atgCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTa                                                 >  1:598623/1‑141 (MQ=25)
                                                                                                  aTTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATTTGa                                           >  1:31659/1‑141 (MQ=21)
                                                                                                  aTTCCGTGGCATGGCGAAAATCCATAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCATAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGa                                           >  1:555774/1‑141 (MQ=255)
                                                                                                       gTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTg                                      <  1:642725/141‑1 (MQ=255)
                                                                                                        gggCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGa                                     <  1:100253/140‑1 (MQ=255)
                                                                                                         ggCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGAt                                    >  2:726129/1‑141 (MQ=38)
                                                                                                            aTGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCtt                                 <  1:649167/141‑1 (MQ=14)
                                                                                                            aTGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCtt                                 <  2:454604/141‑1 (MQ=255)
                                                                                                            aTGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCtt                                 <  1:665036/141‑1 (MQ=255)
                                                                                                            aTGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTGTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCtt                                 <  2:360465/141‑1 (MQ=255)
                                                                                                            aTGGCGAAAAACCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCtt                                 <  2:419738/141‑1 (MQ=255)
                                                                                                              ggCGAACATCCGTAGCTCTGAAGAGCACTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCAAATTAACTCCAGTTTAATATGACGGTGATCTTGc                               <  1:184183/141‑1 (MQ=11)
                                                                                                              ggCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGc                               <  2:603836/141‑1 (MQ=39)
                                                                                                              ggCCAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGc                               <  2:491942/141‑1 (MQ=255)
                                                                                                               gggAAAATCCGTAGATCTGAAGAGAACTACGGTACTGTTTATATAGAGCGTAAATACATTCAATACCCTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCt                              >  2:151919/3‑141 (MQ=11)
                                                                                                               gCGAAAATCCCTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCt                              <  1:289982/141‑1 (MQ=255)
                                                                                                                cGAAAATCCGTAGAACTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGAATCTTTTAAACCTTGTCTTTCTTACTTTAATTTGACGGTGATGTTGccc                             >  2:383120/1‑139 (MQ=11)
                                                                                                                 gAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCa                            >  2:275121/1‑141 (MQ=255)
                                                                                                                  aaaaTCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCaa                           <  1:130817/141‑1 (MQ=255)
                                                                                                                  aaaaTCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCaa                           <  1:762860/141‑1 (MQ=255)
                                                                                                                  aaaaTCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATAGAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCaa                           <  1:279522/141‑1 (MQ=255)
                                                                                                                   aaaTCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAAt                          >  1:74957/1‑141 (MQ=255)
                                                                                                                   aaaTCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAAt                          >  1:679517/1‑141 (MQ=255)
                                                                                                                    aaTCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATg                         <  1:734642/141‑1 (MQ=255)
                                                                                                                     aTCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGa                        <  1:52254/141‑1 (MQ=39)
                                                                                                                     aTCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTACTATGACGTTGGTCTTGCTCAATGa                        >  2:692294/1‑141 (MQ=255)
                                                                                                                     aTCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGa                        <  2:715477/141‑1 (MQ=255)
                                                                                                                     aTCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATTCCTTTTAAAGTATTCTTTGCCTTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGa                        <  2:258928/141‑1 (MQ=14)
                                                                                                                      tCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAg                       <  2:618949/141‑1 (MQ=255)
                                                                                                                       ccGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAgg                      <  2:763292/141‑1 (MQ=255)
                                                                                                                       ccGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAgg                      <  1:238922/141‑1 (MQ=255)
                                                                                                                       ccGTAGATCTGAAGAGACCTACGGATCTTTTTATATAGAGCGTACATACATTCCCTACCTTTTACAGTACTCTACGCCGTATTGATACTTAGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAgg                      >  2:181554/1‑141 (MQ=11)
                                                                                                                        ctgggATTTGAATAGACCTGCCGGTCTTTCTATATAGAGCGCAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGt                     <  1:98377/137‑1 (MQ=11)
                                                                                                                        cGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGt                     >  1:390106/1‑141 (MQ=255)
                                                                                                                        cGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGGGATCTTGCTCAATGAGGt                     >  1:635945/1‑141 (MQ=255)
                                                                                                                        cGTAGATCTGAAGAAAGCTGCGGTTCATAACATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGt                     >  2:585556/1‑141 (MQ=11)
                                                                                                                         gTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGtt                    >  2:324694/1‑141 (MQ=255)
                                                                                                                            gATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTAtt                 <  2:131184/141‑1 (MQ=255)
                                                                                                                              tCTGAAGATACCTGCGGTTCTTTTTATATCGAGCGTACATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCa               <  1:710492/141‑1 (MQ=255)
                                                                                                                              tCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCa               >  2:439357/1‑141 (MQ=255)
                                                                                                                               tttaaGTGACCTGCGGTTCTTTTTATATAGTGCGTAAATACACTCAATACCTTTTTAAGTATTCTCTGCTGTATTGATACTTTGATACCTTGGCCTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAg              <  2:665353/138‑1 (MQ=11)
                                                                                                                               cTGAAGAGACCTGCGGTTCTTTTTATATAGCGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAg              <  2:428765/141‑1 (MQ=255)
                                                                                                                                tGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGa             <  1:616708/141‑1 (MQ=255)
                                                                                                                                tGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGa             <  1:591640/141‑1 (MQ=255)
                                                                                                                                tGAAGAGACCCGCGGTTCTTTTTATATAGTGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTGACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGa             <  2:196031/141‑1 (MQ=255)
                                                                                                                                  gagagACCTGCGGTTCTTTTTATATAGGGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGata           <  1:263580/140‑1 (MQ=255)
                                                                                                                                  aaGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTACTCAATGAGGTTATTCAGata           >  1:625449/1‑141 (MQ=255)
                                                                                                                                  aaGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAAAATTCTTAGCTGTATTGATACTTTGATACCTTGTGTTTCTTACTTTAATTTGACGGTGGTCTTGCGCAATGAGGTTAGTAAGata           >  2:304157/1‑141 (MQ=11)
                                                                                                                                   agagACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTCCTCTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGatat          <  1:532023/141‑1 (MQ=255)
                                                                                                                                   agagACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGatat          <  1:606815/141‑1 (MQ=38)
                                                                                                                                    gagaCCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATAtt         >  1:40905/1‑141 (MQ=38)
                                                                                                                                    gagaCCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATAtt         >  2:337280/1‑141 (MQ=255)
                                                                                                                                     agaCCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCCATGAGGTTATTCAGATAttt        >  1:479788/1‑141 (MQ=255)
                                                                                                                                      gaCCTACGGTTCTTTTTATATAGAGAGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATAtttt       >  2:548418/1‑140 (MQ=11)
                                                                                                                                       aCCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCg      >  2:655623/1‑141 (MQ=255)
                                                                                                                                        ccTACGGTTCTTTTTATATAGAGCGTAAATACATTCAAAACCTATTAAAGTATTCTTTGCCGTCTTGATACTTTGATATCTTGTCTTTCTTACTTTAATATTACGGTGATCTTGCTCAATGAGGTTATTCCTATATTTCgg     >  2:341146/1‑140 (MQ=11)
                                                                                                                                           gCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGt  >  1:353193/1‑141 (MQ=255)
                                                                                                                                           |                                                                                                                                             
ATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGT  >  CP000730/35801‑36080

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: