BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP000730	35,940	G→A	24.5%	R204R (CGC→CGT)	USA300HOU_0026 ←	IS431mec transposase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	35,940	0	G	A	24.5%	597.8 / 244.6	448	R204R (CGC→CGT)	USA300HOU_0026	IS431mec transposase
Reads supporting (aligned to +/- strand): ref base G (138/198); new base A (57/53); total (196/252)
Fisher's exact test for biased strand distribution p-value = 5.95e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.54e-01

Only 100 of 456 total aligned reads displayed.

ATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCT‑TACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGT  >  CP000730/35801‑36080

ttttaataaAAAT‑GATGGTTCTGTTGCAAAGT‑AAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCgg                                                                                                                                            <  2:36853/138‑1 (MQ=11)

aTCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGc                                                                                                                                              <  1:378033/141‑1 (MQ=255)

      taaAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGCAAATCCGTAGATCTGAAGAGACCTGCGGTTCt                                                                                                                                        >  1:108364/1‑141 (MQ=38)

          aaTAGAAGGTTATGATGCAAAGAACCAAAATATAGCTAACTACTAATTTATCATGTCAGGGTTCGCTTAATTTGCGAGCATGATTCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCttttt                                                                                                                                    >  2:534753/1‑141 (MQ=11)

             aGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTtata                                                                                                                                 >  1:687715/1‑141 (MQ=32)

                       ggtgaaaaGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCGGCGGTTCTTTTTATATAGAGCGTaa                                                                                                                       <  2:57164/136‑1 (MQ=0)

                               agtaacaaaTAAAGCTAATCACTAAGATATCATGTCAGTGTTCGTTTAACTGGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACactc                                                                                                               <  1:77199/135‑4 (MQ=1)

                                                            tcttgtgAGTGTTCGTTTAATTTCCTAGCATGATGCTAATTTCGTGGCCTGGCGAAAATCCGTAGATCTGAAGAGACTTGCGGTTCTTTTTATACAGCGCGTGAAGACACTCAATACCTTTCAAAGTATTCTTTGCTGTAt                                                                                  <  2:364006/138‑1 (MQ=1)

                                                                  cAGTGTTCGTCTAACTTGCTAGCATGATGCTAATTTCGTGACATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTGTTATATAGAGCGTAAATAAATTCAATACCTTTTAAAGTATTATTTGCCGAATTGATAc                                                                            >  2:108195/1‑141 (MQ=11)

                                                                   aGTGTTCGTTTAACTAGCTGGCATGATGCTAATTTCGTGGCACGGCGAAAATCCGTAGATCTGATGAGACCTGCCGTTCTTTTTATATAAAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACt                                                                           >  1:577575/1‑141 (MQ=11)

                                                                   aGTGGTCGTTTAGCTTGCTAGCATGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATAGATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACt                                                                           >  1:546951/1‑141 (MQ=11)

                                                                    gtgtTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACtt                                                                          >  2:507829/1‑141 (MQ=255)

                                                                    gtgtTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGAGAAGATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACtt                                                                          <  1:21933/141‑1 (MQ=255)

                                                                     tgtTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACttt                                                                         <  2:400798/141‑1 (MQ=255)

                                                                     tgtTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTATAGTATTATTTGCTGGATTGATACttt                                                                         >  2:740102/1‑141 (MQ=11)

                                                                     tgtTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACttt                                                                         <  2:87287/141‑1 (MQ=17)

                                                                      gtTCGGTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTCGTTCTGAAGAAACCTGCCGTTCTTTTTATATAGAGCGTAGATAGAGCCAATACATTTTAAAGTATTCTTTGCTGTATTGATACTTTg                                                                        >  2:286966/1‑141 (MQ=11)

                                                                      gtTCGCTTCACTTGCTAGCATGATGCTAATTTCGTGGCACGGCGAAAATCCGTAGATCTGCAGAGACCTGCGGTTCTTTTTATATAGACCGTAAATACACTCAATACCTTGTAAAGTATTCTTTGCTGTATTAGTACTTTg                                                                        >  2:486094/1‑141 (MQ=11)

                                                                       ttCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGa                                                                       >  1:144662/1‑141 (MQ=17)

                                                                       ttCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGa                                                                       >  1:170783/1‑141 (MQ=17)

                                                                        tctcTTAACTTGCTAGCATGATGCTAATTTCGTGGCATTGCGAAGATCCGTAGATCTGAAGAGACCTGCTGTTCTTTTTATATAGAGCGTAAATAAAATCAATACATTTTAAAGTATTATTTGCTGTATTGATACTTTTAt                                                                      >  2:211967/4‑141 (MQ=11)

                                                                         cGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATa                                                                     >  1:39537/1‑141 (MQ=32)

                                                                          gtttAACTTGCTAGCATGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATat                                                                    >  1:492431/3‑140 (MQ=11)

                                                                          gCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATAc                                                                    <  1:368981/141‑1 (MQ=17)

                                                                           tttAACTTGCGAGCATGACGCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATatc                                                                   <  2:555603/140‑3 (MQ=11)

                                                                           gttAATTTGCTAGTCTGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGACCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATatc                                                                   <  2:467970/140‑3 (MQ=11)

                                                                            ttAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCt                                                                  <  1:8548/141‑1 (MQ=17)

                                                                             tAACTTGCTATCATGATGCTAATTGCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCtt                                                                 <  1:24598/141‑1 (MQ=12)

                                                                              aaCTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACAAACTATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTg                                                                <  1:338802/141‑1 (MQ=12)

                                                                              aaCTTGCTAGCATGATGCCAATTTCGTGGGATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTATTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACGTTg                                                                <  2:244913/141‑1 (MQ=11)

                                                                               aCTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGt                                                               <  2:642198/141‑1 (MQ=17)

                                                                               aCTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGt                                                               <  1:1495/141‑1 (MQ=17)

                                                                                cTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAGAGTATTCTTTGCTGTATTGATACTTTGATACCTTGtc                                                              >  2:223914/1‑141 (MQ=17)

                                                                                    cTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGtctttt                                                          >  1:724083/1‑140 (MQ=17)

                                                                                     tAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGtctttct                                                         >  1:449787/1‑141 (MQ=32)

                                                                                     tAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGtctttct                                                         <  1:306918/141‑1 (MQ=17)

                                                                                     aaGCAGGAGGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGtctttct                                                         <  2:115687/140‑1 (MQ=255)

                                                                                      aGCATGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGtctttct‑t                                                       <  1:34546/141‑1 (MQ=255)

                                                                                       gCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTACAAATTTCTTTGCTGTATTGATACTTTGATACCTTGtctttct‑tt                                                      >  1:332138/1‑140 (MQ=11)

                                                                                         atgatgCTAATTTCGTGGCATGGGCAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCT‑TACt                                                    <  1:256516/141‑1 (MQ=11)

                                                                                          tgatgCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTGTATATAGTGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCT‑TACtt                                                   <  2:144756/141‑1 (MQ=11)

                                                                                          tgatgCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCT‑TACtt                                                   <  1:427471/141‑1 (MQ=25)

                                                                                          tgatgCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCT‑TACtt                                                   >  2:67500/1‑141 (MQ=21)

                                                                                            atgCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCT‑TACTTTa                                                 >  1:598623/1‑141 (MQ=25)

                                                                                                  aTTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCT‑TACTTTAATTTGa                                           >  1:31659/1‑141 (MQ=21)

                                                                                                  aTTCCGTGGCATGGCGAAAATCCATAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCATAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCT‑TACTTTAATATGa                                           >  1:555774/1‑141 (MQ=255)

                                                                                                       gTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCT‑TACTTTAATATGACGGTg                                      <  1:642725/141‑1 (MQ=255)

                                                                                                        gggCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCT‑TACTTTAATATGACGGTGa                                     <  1:100253/140‑1 (MQ=255)

                                                                                                         ggCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCT‑TACTTTAATATGACGGTGAt                                    >  2:726129/1‑141 (MQ=38)

                                                                                                            aTGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCT‑TACTTTAATATGACGGTGATCtt                                 <  1:649167/141‑1 (MQ=14)

                                                                                                            aTGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCT‑TACTTTAATATGACGGTGATCtt                                 <  2:454604/141‑1 (MQ=255)

                                                                                                            aTGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCT‑TACTTTAATATGACGGTGATCtt                                 <  1:665036/141‑1 (MQ=255)

                                                                                                            aTGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTGTGCTGTATTGATACTTTGATACCTTGTCTTTCT‑TACTTTAATATGACGGTGATCtt                                 <  2:360465/141‑1 (MQ=255)

                                                                                                            aTGGCGAAAAACCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCT‑TACTTTAATATGACGGTGATCtt                                 <  2:419738/141‑1 (MQ=255)

                                                                                                              ggCGAACATCCGTAGCTCTGAAGAGCACTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCAAAT‑TAACTCCAGTTTAATATGACGGTGATCTTGc                               <  1:184183/141‑1 (MQ=11)

                                                                                                              ggCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCT‑TACTTTAATATGACGGTGATCTTGc                               <  2:603836/141‑1 (MQ=39)

                                                                                                              ggCCAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCT‑TACTTTAATATGACGGTGATCTTGc                               <  2:491942/141‑1 (MQ=255)

                                                                                                               gggAAAATCCGTAGATCTGAAGAGAACTACGGTACTGTTTATATAGAGCGTAAATACATTCAATACCCTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCT‑TACTTTAATATGACGGTGATCTTGCt                              >  2:151919/3‑141 (MQ=11)

                                                                                                               gCGAAAATCCCTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCT‑TACTTTAATATGACGGTGATCTTGCt                              <  1:289982/141‑1 (MQ=255)

                                                                                                                cGAAAATCCGTAGAACTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGAATCTTTTAAACCTTGTCTTTCT‑TACTTTAATTTGACGGTGATGTTGccc                             >  2:383120/1‑139 (MQ=11)

                                                                                                                 gAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCT‑TACTTTAATATGACGGTGATCTTGCTCa                            >  2:275121/1‑141 (MQ=255)

                                                                                                                  aaaaTCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCT‑TACTTTAATATGACGGTGATCTTGCTCaa                           <  1:130817/141‑1 (MQ=255)

                                                                                                                  aaaaTCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCT‑TACTTTAATATGACGGTGATCTTGCTCaa                           <  1:762860/141‑1 (MQ=255)

                                                                                                                  aaaaTCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATAGAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCT‑TACTTTAATATGACGGTGATCTTGCTCaa                           <  1:279522/141‑1 (MQ=255)

                                                                                                                   aaaTCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCT‑TACTTTAATATGACGGTGATCTTGCTCAAt                          >  1:74957/1‑141 (MQ=255)

                                                                                                                   aaaTCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCT‑TACTTTAATATGACGGTGATCTTGCTCAAt                          >  1:679517/1‑141 (MQ=255)

                                                                                                                    aaTCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCT‑TACTTTAATATGACGGTGATCTTGCTCAATg                         <  1:734642/141‑1 (MQ=255)

                                                                                                                     aTCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCT‑TACTTTAATATGACGGTGATCTTGCTCAATGa                        <  1:52254/141‑1 (MQ=39)

                                                                                                                     aTCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCT‑TACTTTACTATGACGTTGGTCTTGCTCAATGa                        >  2:692294/1‑141 (MQ=255)

                                                                                                                     aTCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCT‑TACTTTAATATGACGGTGATCTTGCTCAATGa                        <  2:715477/141‑1 (MQ=255)

                                                                                                                     aTCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATTCCTTTTAAAGTATTCTTTGCCTTATTGATACTTTGATATCTTGTCTTTCT‑TACTTTAATATGACGGTGATCTTGCTCAATGa                        <  2:258928/141‑1 (MQ=14)

                                                                                                                      tCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCT‑TACTTTAATATGACGGTGATCTTGCTCAATGAg                       <  2:618949/141‑1 (MQ=255)

                                                                                                                       ccGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCT‑TACTTTAATATGACGGTGATCTTGCTCAATGAgg                      <  2:763292/141‑1 (MQ=255)

                                                                                                                       ccGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCT‑TACTTTAATATGACGGTGATCTTGCTCAATGAgg                      <  1:238922/141‑1 (MQ=255)

                                                                                                                       ccGTAGATCTGAAGAGACCTACGGATCTTTTTATATAGAGCGTACATACATTCCCTACCTTTTACAGTACTCTACGCCGTATTGATACTTAGATATCTTGTCTTTCT‑TACTTTAATATGACGGTGATCTTGCTCAATGAgg                      >  2:181554/1‑141 (MQ=11)

                                                                                                                        ctgggATTTGAATAGACCTGCCGGTCTTTCTATATAGAGCGCAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCT‑TACTTTAATATGACGGTGATCTTGCTCAATGAGGt                     <  1:98377/137‑1 (MQ=11)

                                                                                                                        cGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCT‑TACTTTAATATGACGGTGATCTTGCTCAATGAGGt                     >  1:390106/1‑141 (MQ=255)

                                                                                                                        cGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCT‑TACTTTAATATGACGGGGATCTTGCTCAATGAGGt                     >  1:635945/1‑141 (MQ=255)

                                                                                                                        cGTAGATCTGAAGAAAGCTGCGGTTCATAACATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCT‑TACTTTAATATGACGGTGATCTTGCTCAATGAGGt                     >  2:585556/1‑141 (MQ=11)

                                                                                                                         gTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCT‑TACTTTAATATGACGGTGATCTTGCTCAATGAGGtt                    >  2:324694/1‑141 (MQ=255)

                                                                                                                            gATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCT‑TACTTTAATATGACGGTGATCTTGCTCAATGAGGTTAtt                 <  2:131184/141‑1 (MQ=255)

                                                                                                                              tCTGAAGATACCTGCGGTTCTTTTTATATCGAGCGTACATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCT‑TACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCa               <  1:710492/141‑1 (MQ=255)

                                                                                                                              tCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCT‑TACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCa               >  2:439357/1‑141 (MQ=255)

                                                                                                                               tttaaGTGACCTGCGGTTCTTTTTATATAGTGCGTAAATACACTCAATACCTTTTTAAGTATTCTCTGCTGTATTGATACTTTGATACCTTGGCCTTCT‑TACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAg              <  2:665353/138‑1 (MQ=11)

                                                                                                                               cTGAAGAGACCTGCGGTTCTTTTTATATAGCGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCT‑TACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAg              <  2:428765/141‑1 (MQ=255)

                                                                                                                                tGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCT‑TACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGa             <  1:616708/141‑1 (MQ=255)

                                                                                                                                tGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCT‑TACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGa             <  1:591640/141‑1 (MQ=255)

                                                                                                                                tGAAGAGACCCGCGGTTCTTTTTATATAGTGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCT‑GACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGa             <  2:196031/141‑1 (MQ=255)

                                                                                                                                  gagagACCTGCGGTTCTTTTTATATAGGGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCT‑TACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGata           <  1:263580/140‑1 (MQ=255)

                                                                                                                                  aaGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCT‑TACTTTAATATGACGGTGATCTTACTCAATGAGGTTATTCAGata           >  1:625449/1‑141 (MQ=255)

                                                                                                                                  aaGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAAAATTCTTAGCTGTATTGATACTTTGATACCTTGTGTTTCT‑TACTTTAATTTGACGGTGGTCTTGCGCAATGAGGTTAGTAAGata           >  2:304157/1‑141 (MQ=11)

                                                                                                                                   agagACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCT‑TCCTCTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGatat          <  1:532023/141‑1 (MQ=255)

                                                                                                                                   agagACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCT‑TACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGatat          <  1:606815/141‑1 (MQ=38)

                                                                                                                                    gagaCCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCT‑TACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATAtt         >  1:40905/1‑141 (MQ=38)

                                                                                                                                    gagaCCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCT‑TACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATAtt         >  2:337280/1‑141 (MQ=255)

                                                                                                                                     agaCCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCT‑TACTTTAATATGACGGTGATCTTGCTCCATGAGGTTATTCAGATAttt        >  1:479788/1‑141 (MQ=255)

                                                                                                                                      gaCCTACGGTTCTTTTTATATAGAGAGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCT‑TACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATAtttt       >  2:548418/1‑140 (MQ=11)

                                                                                                                                       aCCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCT‑TACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCg      >  2:655623/1‑141 (MQ=255)

                                                                                                                                        ccTACGGTTCTTTTTATATAGAGCGTAAATACATTCAAAACCTATTAAAGTATTCTTTGCCGTCTTGATACTTTGATATCTTGTCTTTCT‑TACTTTAATATTACGGTGATCTTGCTCAATGAGGTTATTCCTATATTTCgg     >  2:341146/1‑140 (MQ=11)

                                                                                                                                           gCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCT‑TACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGt  >  1:353193/1‑141 (MQ=255)

ATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCT‑TACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGT  >  CP000730/35801‑36080

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`