BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP000730	1,929,918	A→G	58.2%	intergenic (‑1001/‑167)	USA300HOU_1794 ← / → USA300HOU_1796	hypothetical protein/hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	1,929,918	0	A	G	58.2%	93.1 / 159.9	153	intergenic (‑1001/‑167)	USA300HOU_1794/USA300HOU_1796	hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand): ref base A (31/32); new base G (51/38); total (83/70)
Fisher's exact test for biased strand distribution p-value = 4.09e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 153 total aligned reads displayed.

ACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCG‑TCAATTTAGACGCAGAG‑AGGAGGTG‑TA‑TAA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAAT‑‑ACAA‑‑AATAGGTGACA‑TAT‑AGCC‑GCACCAATAAAAATCCCCTCACTACCGCAAATAGTGAGGGGATTGGTGTATAAGTAAATACTTATTTTCGTTGTCTTAA  >  CP000730/1929780‑1930054

ccggtgCTTGGAGATTTGCCGATGATATAATAGTAGAGTAGCCTATCTCTCAGGCG‑TCAATTTAGACGCAGAG‑AGGAGGCG‑TA‑TAA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCCTCAGTGGCTGTGCCAtt                                                                                                                                               <  2:209532/138‑1 (MQ=255)

  tgtgCTTGTATATTCGCCGATGATATAATAGGAGAGTCGCCTATCTCTCCGGCG‑TCAATTTAGACGCAGAG‑AGGAGGTG‑TA‑TAA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGc                                                                                                                                             <  2:28513/141‑1 (MQ=255)

  tatgCTTGTATATGCACAGATGGTAAAATAATAGAGTCGCCTATCTCTCAGGCG‑TCAATT‑‑GACGAAGAG‑AGGAGGTG‑CATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCg                                                                                                                                            <  1:698596/139‑1 (MQ=255)

  tatgCTTGTATACTCACAGAAGGTATAATAATAGAGTCGCCTATCTCTCAGGCG‑TCAATT‑‑GACGAAGAG‑AGGAGGTG‑CATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCg                                                                                                                                            <  1:567610/139‑1 (MQ=255)

   gtgCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCG‑TCAATTTAGACGCAGAG‑AGGGGGCG‑GA‑CAA‑G‑C‑AGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATGGCg                                                                                                                                            >  1:177912/1‑141 (MQ=255)

   gtgCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCG‑TCAATTTAGACGCAGAG‑AGGAGGTG‑TA‑TAA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCg                                                                                                                                            >  2:402339/1‑141 (MQ=255)

   gtgCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCT‑AAAGAAGCAAAAGTGAGCCG‑AGTGAACGTCGAG‑TA‑TAA‑G‑G‑TGATGCTTGTTTTCGTTCACATCAGAGCACCAGTCATCAGTGGCTGTACCATTGCg                                                                                                                                            >  1:370522/1‑141 (MQ=255)

   atgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCG‑TCAATT‑‑GACGAAGAG‑AGGAGGTG‑CATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGt                                                                                                                                           <  1:105831/140‑1 (MQ=255)

      cTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCG‑TCAATT‑‑GACGAAGAG‑AGGAGGTG‑CATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGtatt                                                                                                                                        >  2:642691/1‑141 (MQ=255)

         gTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCG‑TCAATT‑‑GACGAAGAG‑AGGAGGTG‑CATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGtattata                                                                                                                                     <  1:538346/141‑7 (MQ=255)

            tattcacagatggtATAATAATAGAGTCGCCTATCTCTCAGGCG‑TCAATT‑‑GACGAAGAG‑AGGAGGTG‑CA‑TTA‑C‑TTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACtt                                                                                                                                  >  1:1491/9‑141 (MQ=255)

            tatTTACAGATGGTGTAATAATAGAGTCGCCTATCTCTCAGGCG‑TCAATT‑‑GACGAAGAG‑AGGAGGTG‑CATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGtattatactt                                                                                                                                  <  1:198709/141‑7 (MQ=255)

             attcacagatggtATAATAATAGAGTCGCCTATCTCTCAGGCG‑TCAATT‑‑GACGAAGAG‑AGGAGGTG‑CA‑TTACT‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCCGTCATCAGTGGCTGTGCTGTTGCGTATTATACttt                                                                                                                                 >  2:170795/8‑140 (MQ=255)

             aattaccaatgatATAATAGTAGAGTCGCCTATCTCTCAGGCG‑TCAATTTAGACGCAGAG‑AGGAGGTG‑TA‑TAA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCtt                                                                                                                                  <  1:333251/133‑1 (MQ=255)

              ttcacagatgatATAAAAATAGAGTCGCCTATCTCTCAGGCG‑TCAATT‑‑GACGAAGAG‑AGGAGGTG‑CATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTAt                                                                                                                                <  2:23651/135‑1 (MQ=255)

               tcacagatggtATAATAATAGAGTCGCCTATCTCTCAGGCG‑TCAATT‑‑GACGAAGAG‑AGGAGGTG‑CATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTAtt                                                                                                                               >  1:736519/6‑141 (MQ=255)

               tcacagatggtATAATAATAGAGTCGCCTATCTCTCAGGCG‑TCAATT‑‑GACGAAGAG‑AGGAGGTG‑CATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTAtt                                                                                                                               >  1:734401/6‑141 (MQ=255)

                 acagatggtATAATAATAGAGTCGCCTATCTCTCAGGCG‑TCAATT‑‑GACGAAGAG‑AGGAGGTG‑CATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTgg                                                                                                                             >  1:687411/4‑141 (MQ=255)

                 acagatggtATAATAATAGAGTCGCCTATCTCTCAGGCG‑TCAATT‑‑GACGAAGAG‑AGGAGGTG‑CATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCGGTGCTGTTGCGTATTATACTTATTgg                                                                                                                             <  2:585235/138‑1 (MQ=255)

                  cagatggtATAATAATAGAGAAGCATAACTCTCTTACG‑TCAATT‑‑GACGAAGAG‑AGGAGGTG‑CATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGc                                                                                                                            >  1:767767/3‑141 (MQ=255)

                   agttggtataATAATAGAGTCGCCTAGCTCTCAGGCG‑TCAATT‑‑GACGAAGAG‑AGGAGGTG‑CATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCt                                                                                                                           <  1:639072/135‑1 (MQ=255)

                   agatggtATAATAATAGAGTCGCCTATCTCTCAGGCG‑TCAATT‑‑GACGAAGAG‑AGGAGGTG‑CATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCt                                                                                                                           >  2:211307/2‑141 (MQ=255)

                      tggtataATAATAGAGTCGCCTATCTCTCAGGCG‑TCAATT‑‑GACGAAGAG‑AGGAGGTG‑CC‑TTACT‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAg                                                                                                                        >  1:297551/4‑141 (MQ=255)

                      tggtataATAATAGAGTCGCCTATCTCTCAGGCG‑TCAATT‑‑GACGAAGAG‑AGGAGGTG‑CATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAg                                                                                                                        >  1:324897/4‑141 (MQ=255)

                      tggtataATAATAGAGTCGCCTATCTCTCAGGCG‑TCAATT‑‑GACGAAGAG‑AGGAGGTG‑CA‑TTACT‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCttag                                                                                                                        <  2:425958/138‑4 (MQ=255)

                      tggtataATAATAGAGTCGCCTATCTCTCAGGCG‑TCAATT‑‑GACGAAGAG‑AGGAGGTG‑CA‑TTACT‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAg                                                                                                                        >  1:430400/4‑141 (MQ=255)

                      tggtataATAATAGAGGCGCCTATCTCTCAGGCG‑TCAATT‑‑GACGCAGAG‑AGGAGGAG‑CATTAC‑T‑T‑TGCTAATCATTTTAGTTCACATAATAGCACGAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAg                                                                                                                        >  1:646422/4‑141 (MQ=255)

                       ggtataATAATAGAGTCGCCTATCTCTCAGGCG‑TCAATT‑‑GACGAAGAG‑AGGAGGTG‑CATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGt                                                                                                                       >  1:582952/3‑141 (MQ=255)

                       ggtataATAATAGAGTCGCCTATCTCTCAGGCG‑TCAATT‑‑GACGAAGAG‑AGGAGGTG‑CATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGt                                                                                                                       >  1:409270/3‑141 (MQ=255)

                       ggtataAAAATAGAGTCGCCAATCTCTCAGGCG‑TCAATT‑‑GACGAAGAG‑AGGAGGTG‑CATTAC‑T‑T‑TGCTAATCATTTTCGTTCGCATCATAGCACCAGTCATCAGTGGCTGTGCTGTTACGTATTATACTTATTGGCTTAGt                                                                                                                       >  1:488767/3‑141 (MQ=255)

                         tataATAGTAGAGTCGCCTATCTCTCAGGCG‑TCAATTTAGACGCAGAG‑AGGAGGTG‑TA‑TAA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTa                                                                                                                      >  2:697637/1‑141 (MQ=255)

                          ataataataGAGTCGCCTATCTCTCAGGCG‑TCAATT‑‑GACGAAGAG‑AGGAGGTG‑CATTAC‑T‑T‑TGCTACTCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTaaa                                                                                                                    <  2:576585/141‑3 (MQ=255)

                           aaataatAGAGTCGCCTATCTCTCAGGCG‑TCAATT‑‑GACGAAGAG‑AGGAGGTG‑CA‑TTA‑C‑TTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTaaac                                                                                                                   <  2:386510/140‑4 (MQ=255)

                             ataataGAGTCGCCTATCTCTCAGGCG‑TCAATT‑‑GACGAAGAG‑AGGAGGTG‑CATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTATACGc                                                                                                                 <  1:525159/141‑1 (MQ=255)

                             ataataGAGTCGCCTATCTCTCAGGCG‑TCAATT‑‑GACGAAGAG‑AGGAGGTG‑CATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGc                                                                                                                 <  2:158035/141‑1 (MQ=255)

                             ataGTAGAGTCGCCTATCTCTCAGGCG‑TCAATTTAGACGCAGAG‑AGGAGGTG‑TA‑TAA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACg                                                                                                                  <  1:247138/141‑1 (MQ=255)

                             agtatagAGTCGCCTATCTCTCAGGCG‑TCAATT‑‑GACGAAGAG‑AGGAGGTG‑CATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGc                                                                                                                 <  1:632896/137‑1 (MQ=255)

                               aatagATTCGCCTATCTCTCAGGCG‑TCAA‑‑TGGACTAAGAG‑AGGAGGTG‑CATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaa                                                                                                               <  2:770001/139‑1 (MQ=255)

                               aatagAGTCGCCTATCTCTCAGGCG‑TCAATT‑‑GACGAAGAG‑AGGAGGTG‑CA‑TTACT‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaa                                                                                                               <  2:749139/139‑1 (MQ=255)

                                atagAGTCGCCTATCTCTCAGGCG‑TCAATT‑‑GACGAAGAG‑AGGAGGTG‑CATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaac                                                                                                              >  2:565761/2‑140 (MQ=255)

                                 tagAGTCGCCTATCTCTCAGGCG‑TCAATT‑‑GACGAAGAG‑AGGAGGTG‑CATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaaaa                                                                                                             >  1:31180/1‑139 (MQ=255)

                                 tagAGTCGCCTATCTCTCAGGCG‑TCAATT‑‑GACGAAGAG‑AGGAGGTG‑CA‑TTACT‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaaca                                                                                                             >  2:758991/1‑139 (MQ=255)

                                 atgagTCGCCTATCTCTCAGGCG‑TCAATT‑‑GACGAAGAG‑AGGAGGTG‑CATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaaca                                                                                                             <  1:544764/139‑3 (MQ=255)

                                  agagTCGCCTATCTCTCAGGCG‑TCAATTTAGACGCAGAG‑AGGAGGTG‑TA‑TAA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAAT‑‑a                                                                                                           <  2:478470/141‑1 (MQ=255)

                                  agagTCGCCTATCTCTCAGGCG‑TCAATTTAGACGCAGAG‑AGGAGGTG‑TA‑TAA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAAT‑‑a                                                                                                           <  2:668581/141‑1 (MQ=255)

                                  agagTCGCCTATCTCTCAGGCG‑TCAATT‑‑GACGAAGAG‑AGGAGGTG‑CATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaa                                                                                                            <  1:244552/141‑4 (MQ=255)

                                   gagTCGCCTATCTCTCAGGCG‑TCAATT‑‑GACGAAGAG‑AGGAGGTG‑CATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCGGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaa                                                                                                           >  1:59597/1‑137 (MQ=255)

                                   gagTCGCCTATCCCTCAGGCG‑TCAATTTAGACGCAGAG‑AGGAGGTG‑TA‑TAA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAAT‑‑Ac                                                                                                          <  2:41598/141‑1 (MQ=255)

                                    agTCGCCTATCTCTCAGGCG‑TCAATT‑‑GACGAAGAG‑AGGAGGTG‑CATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaat                                                                                                          >  2:72282/1‑136 (MQ=255)

                                    agTCGCCTATCTCTCAGGCG‑TCAATT‑‑GACGAAGAG‑AGGAGGTG‑CA‑TTACT‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaat                                                                                                          >  2:480994/1‑136 (MQ=255)

                                     gTCGCCTATCTCTCAGGCG‑TCAATTTAGACGCAGAG‑AGGAGGTG‑TA‑TAA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAAT‑‑ACaa                                                                                                        <  1:72687/141‑1 (MQ=255)

                                     gTCGCCTATCTCTCAGGCG‑TCAATTTAGACGCAGAG‑AGGAGGTG‑TA‑TAA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAAT‑‑ACaa                                                                                                        <  2:32723/141‑1 (MQ=255)

                                     gTCGCCTATCTCTCAGGCG‑TCAATTTAGACGCAGAG‑AGGAGGTG‑TA‑TAA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAAT‑‑ACaa                                                                                                        <  1:492273/141‑1 (MQ=255)

                                     gTCGCCGATCTCTCAGGCG‑TCAATT‑‑GAAGAAGAG‑AGGAGGTGCGT‑TAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaata                                                                                                         >  2:196975/1‑135 (MQ=255)

                                      tCGCCTATCTCTCAGGCG‑TCAATT‑‑GACGAAGAG‑AGGAGGTG‑CATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataa                                                                                                        >  2:113229/1‑134 (MQ=255)

                                        gCCTATCTCTCAGGCG‑TCAATT‑‑GACGAAGAG‑AGGAGGTG‑CATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataatt                                                                                                      >  1:287339/1‑132 (MQ=255)

                                        gCCTATCTCTCAGGCG‑TCAATT‑‑GACGAAGAG‑AGGAGGTG‑CATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataatt                                                                                                      <  2:154858/141‑10 (MQ=255)

                                        gCCTATCTCTCAGGCG‑TCAATT‑‑GACGAAGAG‑AGGAGGTG‑CATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataatt                                                                                                      >  2:24855/1‑132 (MQ=255)

                                        gCCTATCTCTCAGGCG‑TCAATT‑‑GACGAAGAG‑AGGAGGTG‑CATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataatt                                                                                                      <  2:231325/141‑10 (MQ=255)

                                         ccTATCTCTCAGGCG‑TCAATTTAGACGCAGAG‑AGGAGGTG‑TA‑TAA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAAT‑‑ACAA‑‑AATa                                                                                                  >  2:193899/1‑141 (MQ=255)

                                         ccTATCTCTCAGGCG‑TCAATT‑‑GACGAAGAG‑AGGAGGTG‑CATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataatta                                                                                                     >  1:667696/1‑131 (MQ=255)

                                         ccTATCTCTCAGGCG‑TCAATT‑‑GACGAAGAG‑AGGAGGTG‑CATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataatta                                                                                                     >  2:514869/1‑131 (MQ=255)

                                         ccTATCTCTCAGGCG‑TCAATT‑‑GACGAAGAG‑AGGAGGTG‑CATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataatta                                                                                                     >  1:491941/1‑131 (MQ=255)

                                         ccTATCTCTCAGGCG‑TCAATT‑‑GACGAAGAG‑AGGAGGTG‑CA‑TTACT‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataatta                                                                                                     >  2:616714/1‑131 (MQ=255)

                                         ccTATCTCTCAGGCG‑TCAATT‑‑GACGAAGAG‑AGGAGGTG‑CA‑TTACT‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataatta                                                                                                     >  1:457055/1‑131 (MQ=255)

                                          cTATCTCTCAGGCG‑TCAATT‑‑GACGAAGAG‑AGGAGGTG‑CATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataattaa                                                                                                    >  2:85138/1‑130 (MQ=255)

                                          cTATCTCTCAGGCG‑TCAATT‑‑GACGAAGAG‑AGGAGGTG‑CATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataattaa                                                                                                    >  1:320741/1‑130 (MQ=255)

                                           tATCTCTCAGGCG‑TCAATT‑‑GTCGAAGAG‑AGGAGGTG‑CA‑TTA‑CTT‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataattaat                                                                                                   <  2:71437/141‑13 (MQ=255)

                                           tATCTCTCAGGCG‑TCAATT‑‑GACGAAGAG‑AGGAGGTG‑CATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataattaat                                                                                                   <  2:613140/141‑13 (MQ=255)

                                           tATCTCTCAGGCG‑TCAATT‑‑GACGAAGAG‑AGGAGGTG‑CATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataattaaa                                                                                                   >  1:694320/1‑129 (MQ=255)

                                            aTCTCTCAGGCG‑TCAATT‑‑GACGAAGAG‑AGGAGGTG‑CATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataattaata                                                                                                  >  2:277348/1‑128 (MQ=255)

                                            aTCTCTCAGGCG‑TCAATT‑‑GACGAAGAG‑AGGAGGTG‑CATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataagtaata                                                                                                  >  1:171442/1‑128 (MQ=255)

                                            aTCTCTCAGGCG‑TCAATT‑‑GACGAAGAG‑AGGAGGTG‑CA‑TTA‑C‑TTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataattaata                                                                                                  >  2:666719/1‑128 (MQ=255)

                                                 tcAGGCG‑TCAATTTAGACGCAGAG‑AGGAGGTG‑TA‑TAA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAAT‑‑ACAA‑‑AATAGGTGACa‑t                                                                                         >  1:608235/1‑141 (MQ=255)

                                                 tcAGGCG‑TCAATTTAGACGCAGAG‑AGGAGGAG‑TA‑TAA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTAGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAAT‑‑ACAA‑‑CATGGGTGGCa‑t                                                                                         >  2:256390/1‑141 (MQ=255)

                                                 ccAGGCG‑GCAATTTAGACGCAGAG‑AGGAGGGG‑TA‑TAA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTAGTGGCTAAGTAGACGCAAT‑‑ACAA‑‑AATAGGTGACa‑t                                                                                         <  1:342103/140‑1 (MQ=255)

                                                                 gACGCAGAG‑AGGAGGTG‑TA‑TAA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAAT‑‑ACAA‑‑AATAGGTGACA‑TAT‑AGCC‑GCACCAATa                                                                        >  1:456067/1‑141 (MQ=255)

                                                                  aCGCAGAG‑AGGAGGTG‑TA‑TAA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTGTTATTCGGCCTGCCTAAATCGGCGCAAT‑‑ACAA‑‑AATAGGTGACA‑TAT‑AGCC‑GCCCCAAGaa                                                                       >  2:316106/1‑141 (MQ=255)

                                                                     cAGAG‑CGGAACTG‑TG‑TAA‑G‑C‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAAT‑‑ACAA‑‑AATAGGTGACA‑TAT‑AGCC‑GCACCAATaaaaa                                                                    <  1:756816/141‑1 (MQ=255)

                                                                           aggaggTG‑TG‑GAA‑G‑G‑TGATGCTTAGTTTCGTTCACATCATAGCACCCGTCATCAGGGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAAT‑‑CCAA‑‑AATAGGTGACA‑TAT‑AGCC‑GCACCAATAAAAATcctc                                                               >  1:566864/1‑139 (MQ=255)

                                                                             gaggTG‑TA‑TAA‑G‑G‑TGATGATAGGGTGCTGTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAAT‑‑ACAA‑‑AATAGGTGACA‑TAT‑AGCC‑GCACCAATAAAAATCCCCTc                                                             >  2:748512/1‑141 (MQ=255)

                                                                                      ttaA‑G‑G‑TCGTGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAAT‑‑ACAA‑‑AATAGGTGACA‑TAT‑AGCC‑GCACCAATAAAAATCCCCTCACTACCg                                                      <  2:539066/140‑1 (MQ=255)

                                                                                              gttaTGCTTGTTTTCGTTCACATCATAGCCCCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAAT‑‑ACAA‑‑AATAGGTGACA‑TAT‑AGCC‑GCACCAATAAAAATCCCCTCACTACCGCAAAt                                                 <  2:638656/138‑1 (MQ=255)

                                                                                                  tGCTTATATTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAAG‑‑ACAA‑‑AATAGGTGACA‑TAT‑AGCC‑GCACCAATAAAAGTCCCCTCACTACCGCAAATAGTg                                             >  1:487649/1‑141 (MQ=255)

                                                                                                      tATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAAT‑‑ACAA‑‑AATAGGTGACA‑TAT‑AGCC‑GCACCAATAAAAATCCCCTCACTACCGCAAATAGTGAggg                                         <  2:370522/141‑1 (MQ=255)

                                                                                                          ttCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAAT‑‑ACAA‑‑AATAGGTGACA‑TAT‑AGCC‑GCACCAATAAAAATCCCCTCACTACCGCAAATAGTGAGGGGAtt                                     <  1:276995/141‑1 (MQ=255)

                                                                                                            cGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAAT‑‑ACAA‑‑AATAGGTGACA‑TAT‑AGCC‑GCACCAATAAAAATCCCCTCACTACCGCAAATAGTGAGGGGATTgg                                   >  1:237177/1‑141 (MQ=255)

                                                                                                            cGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACCCAAA‑‑ACAA‑‑AATCGGTGACA‑TAT‑AGCC‑GCACCAATAAAAATCCCCTCACTACCGCAAATAGTGAAGGGATTgg                                   >  1:626273/1‑141 (MQ=255)

                                                                                                              ttCACATCATAGCACCAGTCATCAGTGGCGGTGCGATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAAT‑‑ACAA‑‑AATAGGGGACA‑TAC‑AGCC‑GCACCAATAAAAATCCCCTCACTACCGCAAATAGTGAGGGGCTTGgtg                                 <  2:268565/141‑1 (MQ=255)

                                                                                                                  catcatAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAAT‑‑ACAA‑‑AATAGGTGACA‑TAT‑AGCC‑GCACCAATAAAAATCCCCTCACTACCGCAAATAGTGAGGGGATTGGTGtata                             >  2:291179/1‑141 (MQ=255)

                                                                                                                       tAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCAACAAATAATTAATAGGCGACTTTATAAGCCAGCATCGA‑AAAAATCCCCACACTACTGC‑AATAGTGTGGGGATTGGTGcatt                             >  2:677055/1‑140 (MQ=255)

                                                                                                                         gCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAAT‑‑ACAA‑‑GATAGGTGACA‑TAT‑AGCC‑GCACCAATAAAAATCCCCTCACTACCGCAAATAGTGAGGGGATTGGTGTATAAGTAAAt                      >  1:423073/1‑141 (MQ=255)

                                                                                                                          cACCGGTCATCAGTGGCTGTGCCATGGCGTTTTTTTCTTATTGGCTAAGTAGACGCAAT‑‑ACAA‑‑AATAGGTGACA‑TAT‑AGCC‑GCACCAATAAACTTCCCCTCACTTCCGCCAATAGTGAGGGGATTGGTGTATCAGTACATa                     <  2:377276/141‑1 (MQ=255)

                                                                                                                              aGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAAT‑‑ACAA‑‑ACTAGGTGACA‑TAT‑AGCC‑GCACCAATAAAAATCCCCTCACTACCGCAAATAGTGAGGGGCTTGGTGTCTAAGTAAATACTTa                 >  2:118788/1‑141 (MQ=255)

                                                                                                                                tcatcaGTGGCTGTGCCATTGCGTTTTTTTCTTCTTGGCTAAGTAGACGCAAT‑‑GCCA‑‑AATAGGTGACA‑TAT‑AGCC‑GCACCAATAAAAATCCCCTCATTACCGCAAATAGTGAGGGGATTGTTGTATAAGTAAATACTTAtt               <  2:423686/141‑1 (MQ=255)

                                                                                                                                tcatcaGTGGCTGGGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAAT‑‑ACAA‑‑AATAGGTGACA‑TAT‑AGCC‑GCACCAATAAAAATCCCCTCACTACCGCAAATAGTGAGGGGATTGGTGTATAAGTAAATACTTAtt               <  2:386619/141‑1 (MQ=255)

                                                                                                                                gcatcaGTGGCTGTGCCATTGCGTTTTTTGCTTATTGGCTAAGTAGACGCAAT‑‑ACAA‑‑AATAGGTGACA‑TAT‑AGCC‑GCACCAATAAAAATCCCCTCACTACCGCAAATAGTGAGGGGATTGGTGTATAAGTAAATACTTAtt               <  1:61050/140‑1 (MQ=255)

                                                                                                                                 catcaGTGGCTGTGCCATTGCGTTTTTTTTTTTTTTTCTAAGTAGACGCAAT‑‑ACAA‑‑AATAGGTGACA‑TAT‑AGCC‑GCACCAATAAAAATCCCCTCACTACCGCAAATAGTGAGGGGATTGGTGTATAAGTAAATACTTAttt              >  2:302004/1‑141 (MQ=255)

                                                                                                                                      gTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAAT‑‑ACAA‑‑AATAGGTGACA‑TAT‑AGCC‑GCACCAATAAAAATCCCCTCACTACCGCAAATAGTGAGGGGATTGGTGTATAAGTAAATACTTATTTTCGtt         <  1:724184/141‑1 (MQ=255)

                                                                                                                                             tgCCATTGCGTTTTTTTCTAATTTGCTAAGTAGACGCAAT‑‑ACAA‑‑AATAGGTGACA‑TAT‑AGCT‑GCACCAATAAAAATCCCCTCACTACCGCAAATAGTGAGGGGATTGGTGTATGCGTAAATCCTTATTTTGGTTGTCTTaa  >  1:409666/1‑141 (MQ=38)

ACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCG‑TCAATTTAGACGCAGAG‑AGGAGGTG‑TA‑TAA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAAT‑‑ACAA‑‑AATAGGTGACA‑TAT‑AGCC‑GCACCAATAAAAATCCCCTCACTACCGCAAATAGTGAGGGGATTGGTGTATAAGTAAATACTTATTTTCGTTGTCTTAA  >  CP000730/1929780‑1930054

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`