BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP000730	1,929,925	T→A	59.6%	intergenic (‑1008/‑160)	USA300HOU_1794 ← / → USA300HOU_1796	hypothetical protein/hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	1,929,925	0	T	A	59.6%	89.0 / 148.3	134	intergenic (‑1008/‑160)	USA300HOU_1794/USA300HOU_1796	hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand): ref base T (26/28); new base A (50/29); total (77/57)
Fisher's exact test for biased strand distribution p-value = 1.08e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.80e-01

Only 100 of 139 total aligned reads displayed.

GCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTG‑TA‑TAA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCA‑‑AT‑‑ACAA‑‑AATAGGTGAC‑A‑TAT‑AGCC‑GCACCAATAAAAATCCCCTCACTACCGCAAATAGTGAGGGGATTGGTGTATAAGTAAATACTTATTTTCGTTGTCTTAATTATACTG  >  CP000730/1929785‑1930062

gCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTG‑TA‑TAA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGtt                                                                                                                                                     >  1:691625/1‑141 (MQ=255)

   gttattTTGGCCGATGATATAATAGTAGAGTCGCTTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTG‑TA‑TAA‑G‑T‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGttttt                                                                                                                                                  <  2:395127/139‑1 (MQ=255)

       tattcacagatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CA‑TTA‑C‑TTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACtt                                                                                                                                             >  1:1491/9‑141 (MQ=255)

       tatTTACAGATGGTGTAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGtattatactt                                                                                                                                             <  1:198709/141‑7 (MQ=255)

        attcacagatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CA‑TTACT‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCCGTCATCAGTGGCTGTGCTGTTGCGTATTATACttt                                                                                                                                            >  2:170795/8‑140 (MQ=255)

        aattaccaatgatATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTG‑TA‑TAA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCtt                                                                                                                                             <  1:333251/133‑1 (MQ=255)

          tcacagatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTAtt                                                                                                                                          >  1:734401/6‑141 (MQ=255)

          tcacagatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTAtt                                                                                                                                          >  1:736519/6‑141 (MQ=255)

            acagatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTgg                                                                                                                                        >  1:687411/4‑141 (MQ=255)

            acagatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCGGTGCTGTTGCGTATTATACTTATTgg                                                                                                                                        <  2:585235/138‑1 (MQ=255)

             cagatggtATAATAATAGAGAAGCATAACTCTCTTACGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGc                                                                                                                                       >  1:767767/3‑141 (MQ=255)

              agttggtataATAATAGAGTCGCCTAGCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCt                                                                                                                                      <  1:639072/135‑1 (MQ=255)

               gatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCtt                                                                                                                                     <  1:165398/141‑2 (MQ=255)

               gatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCtt                                                                                                                                     >  2:489279/1‑140 (MQ=255)

                atggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CA‑TTA‑C‑TTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCtta                                                                                                                                    <  2:753092/141‑3 (MQ=255)

                 tggtataATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CC‑TTACT‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAg                                                                                                                                   >  1:297551/4‑141 (MQ=255)

                 tggtataATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CA‑TTACT‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCttag                                                                                                                                   <  2:425958/138‑4 (MQ=255)

                 tggtataATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CA‑TTACT‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAg                                                                                                                                   >  1:430400/4‑141 (MQ=255)

                 tggtataATAATAGAGGCGCCTATCTCTCAGGCGTCAATT‑‑GACGCAGAGAGGAGGAG‑CATTAC‑T‑T‑TGCTAATCATTTTAGTTCACATAATAGCACGAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAg                                                                                                                                   >  1:646422/4‑141 (MQ=255)

                 gggtataATAAGAGAGTCGCCTATCTCCCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑TGCGAATCATTTTCGTTCACATCATAGCCCCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCttag                                                                                                                                   <  1:261843/138‑4 (MQ=255)

                  ggtataATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGt                                                                                                                                  >  1:409270/3‑141 (MQ=255)

                  ggtataATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGt                                                                                                                                  >  1:582952/3‑141 (MQ=255)

                    tataATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTG‑TA‑TAA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTa                                                                                                                                 >  2:697637/1‑141 (MQ=255)

                    tataATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTaa                                                                                                                                <  1:538867/141‑2 (MQ=255)

                    tataATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CA‑TTACT‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTaa                                                                                                                                >  2:668643/1‑140 (MQ=255)

                     ataataataGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑TGCTACTCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTaaa                                                                                                                               <  2:576585/141‑3 (MQ=255)

                      aaataatAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CA‑TTA‑C‑TTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTaaac                                                                                                                              <  2:386510/140‑4 (MQ=255)

                        ataataGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTATACGc                                                                                                                            <  1:525159/141‑1 (MQ=255)

                        ataataGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGc                                                                                                                            <  2:158035/141‑1 (MQ=255)

                        ataataGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGc                                                                                                                            <  1:293933/141‑1 (MQ=255)

                        ataGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTG‑TA‑TAA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACg                                                                                                                             <  1:247138/141‑1 (MQ=255)

                        agtatagAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGc                                                                                                                            <  1:632896/137‑1 (MQ=255)

                          aatagATTCGCCTATCTCTCAGGCGTCAA‑‑TGGACTAAGAGAGGAGGTG‑CATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCa‑‑a                                                                                                                        <  2:770001/139‑1 (MQ=255)

                          aatagAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CA‑TTACT‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCa‑‑a                                                                                                                        <  2:749139/139‑1 (MQ=255)

                           atagAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCa‑‑ac                                                                                                                       >  2:565761/2‑140 (MQ=255)

                             agagTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTG‑TA‑TAA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCA‑‑AT‑‑a                                                                                                                    <  2:478470/141‑1 (MQ=255)

                              gagTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCGGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCa‑‑acaaa                                                                                                                    >  1:59597/1‑137 (MQ=255)

                              gagTCGCCTATCCCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTG‑TA‑TAA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCA‑‑AT‑‑Ac                                                                                                                   <  2:41598/141‑1 (MQ=255)

                               agTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCa‑‑acaaat                                                                                                                   >  2:72282/1‑136 (MQ=255)

                               agTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CA‑TTACT‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCa‑‑acaaat                                                                                                                   >  2:480994/1‑136 (MQ=255)

                                gTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTG‑TA‑TAA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCA‑‑AT‑‑ACaa                                                                                                                 <  2:32723/141‑1 (MQ=255)

                                gTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTG‑TA‑TAA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCA‑‑AT‑‑ACaa                                                                                                                 <  1:72687/141‑1 (MQ=255)

                                gTCGCCTATCTCTCAGGCGTCAATT‑‑GACCAAGCGAGGAGGTG‑CATTAC‑T‑T‑TGCTAATCATTTCCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCa‑‑acaaata                                                                                                                  <  1:315589/141‑7 (MQ=255)

                                gTCGCCGATCTCTCAGGCGTCAATT‑‑GAAGAAGAGAGGAGGTGCGT‑TAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCa‑‑acaaata                                                                                                                  >  2:196975/1‑135 (MQ=255)

                                   gCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCCCCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTACACGCa‑‑acaaatgatt                                                                                                               >  1:244536/1‑132 (MQ=255)

                                   gCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCa‑‑acaaataatt                                                                                                               >  2:24855/1‑132 (MQ=255)

                                   gCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCa‑‑acaaataatt                                                                                                               <  2:231325/141‑10 (MQ=255)

                                   gCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCa‑‑acaaataatt                                                                                                               >  1:287339/1‑132 (MQ=255)

                                   gCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCa‑‑acaaataatt                                                                                                               <  2:154858/141‑10 (MQ=255)

                                   gCCTATCTCTAAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCa‑‑acaaataatt                                                                                                               <  2:284388/141‑10 (MQ=255)

                                    ccTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTG‑TA‑TAA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCA‑‑AT‑‑ACAA‑‑AATa                                                                                                           >  2:193899/1‑141 (MQ=255)

                                    ccTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCa‑‑acaaataatta                                                                                                              >  1:491941/1‑131 (MQ=255)

                                    ccTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CA‑TTACT‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCa‑‑acaaataatta                                                                                                              >  1:457055/1‑131 (MQ=255)

                                    ccTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CA‑TTACT‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCa‑‑acaaataatta                                                                                                              >  2:616714/1‑131 (MQ=255)

                                     cTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCa‑‑acaaataattaa                                                                                                             >  2:85138/1‑130 (MQ=255)

                                     cTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCa‑‑acaaataattaa                                                                                                             >  1:320741/1‑130 (MQ=255)

                                     cTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCa‑‑acaaataattaa                                                                                                             >  1:744668/1‑130 (MQ=255)

                                     cTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCa‑‑acaaataattaa                                                                                                             >  2:101946/1‑130 (MQ=255)

                                      tATCTCTCAGGCGTCAATT‑‑GTCGAAGAGAGGAGGTG‑CA‑TTA‑CTT‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCa‑‑acaaataattaat                                                                                                            <  2:71437/141‑13 (MQ=255)

                                      tATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCa‑‑acaaataattaaa                                                                                                            >  1:694320/1‑129 (MQ=255)

                                       aTCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCa‑‑acaaataattaata                                                                                                           >  1:325699/1‑128 (MQ=255)

                                       aTCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCa‑‑acaaataattaata                                                                                                           >  2:277348/1‑128 (MQ=255)

                                       aTCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCa‑‑acaaataagtaata                                                                                                           >  1:171442/1‑128 (MQ=255)

                                       aTCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CA‑TTA‑C‑TTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCa‑‑acaaataattaata                                                                                                           >  2:666719/1‑128 (MQ=255)

                                        tctctcAGGCGTCAATTTAGACGCAGAGAGGAGGTG‑TA‑TAA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTTTTTTTTTTTAAGTAGACGCA‑‑AT‑‑ACAA‑‑AATAGGTg                                                                                                       >  2:205758/1‑141 (MQ=255)

                                            tcAGGCGTCAATTTAGACGCAGAGAGGAGGTG‑TA‑TAA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCA‑‑AT‑‑ACAA‑‑AATAGGTGAC‑a‑t                                                                                                 >  1:608235/1‑141 (MQ=255)

                                            tcAGGCGTCAATTTAGACGCAGAGAGGAGGAG‑TA‑TAA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTAGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCA‑‑AT‑‑ACAA‑‑CATGGGTGGC‑a‑t                                                                                                 >  2:256390/1‑141 (MQ=255)

                                            ccAGGCGGCAATTTAGACGCAGAGAGGAGGGG‑TA‑TAA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTAGTGGCTAAGTAGACGCA‑‑AT‑‑ACAA‑‑AATAGGTGAC‑a‑t                                                                                                 <  1:342103/140‑1 (MQ=255)

                                              aGGCGTCAATTTAGACGCAGAGAGGAGGGG‑TA‑TAA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCA‑‑AT‑‑ACAA‑‑AATAGGGGGC‑a‑tat                                                                                               >  2:587540/1‑141 (MQ=255)

                                                   tCAATTTAGACGCAGAGAGGAGGTG‑TA‑TAA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCA‑‑AT‑‑ACAA‑‑AATAGGTGAC‑A‑TAT‑CGCC‑g                                                                                        >  2:644272/1‑141 (MQ=255)

                                                     aaTTTAGACGCAGAGAGGAGGTG‑TA‑TAA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCA‑‑AT‑‑TCAA‑‑AATAGGTGAG‑A‑TAT‑AGCC‑GCa                                                                                      >  2:341349/1‑141 (MQ=255)

                                                              gctgagcgGAGGTG‑TA‑TAA‑G‑G‑GGATGCTTATTTTCGTTCCCATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCA‑‑AT‑‑ACAA‑‑AATAGGTGAC‑A‑TAT‑AGCC‑GCACCAATaaaa                                                                             <  2:379884/138‑1 (MQ=255)

                                                                    aggaggTG‑TG‑GAA‑G‑G‑TGATGCTTAGTTTCGTTCACATCATAGCACCCGTCATCAGGGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCA‑‑AT‑‑CCAA‑‑AATAGGTGAC‑A‑TAT‑AGCC‑GCACCAATAAAAATcctc                                                                       >  1:566864/1‑139 (MQ=255)

                                                                      gaggTG‑TA‑TAA‑G‑G‑TGATGATAGGGTGCTGTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCA‑‑AT‑‑ACAA‑‑AATAGGTGAC‑A‑TAT‑AGCC‑GCACCAATAAAAATCCCCTc                                                                     >  2:748512/1‑141 (MQ=255)

                                                                         gtgtta‑taA‑G‑G‑TGATGCTTATTTTCTGTCACATCATAGCACCATTCATCAGTGGCTGCGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCA‑‑AT‑‑ACAA‑‑AATAGGTGAC‑A‑TAT‑AGCC‑GTACCAATAAAAATCCCCTAAc                                                                   <  2:299320/137‑1 (MQ=255)

                                                                          tg‑tA‑TAA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCA‑‑AT‑‑ACAA‑‑AATAGGTGAC‑A‑TAT‑AGCC‑GCACCAATAAAAATCCCCTCACTa                                                                 <  2:246895/141‑1 (MQ=255)

                                                                               ttaA‑G‑G‑TCGTGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCA‑‑AT‑‑ACAA‑‑AATAGGTGAC‑A‑TAT‑AGCC‑GCACCAATAAAAATCCCCTCACTACCg                                                              <  2:539066/140‑1 (MQ=255)

                                                                                  a‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCA‑‑AT‑‑ACAA‑‑AATAGGTGAC‑A‑TAT‑AGCC‑GCACCAATAAAAATCCCCTCACTACCGCaa                                                           >  1:489336/1‑141 (MQ=255)

                                                                                       gttaTGCTTGTTTTCGTTCACATCATAGCCCCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCA‑‑AT‑‑ACAA‑‑AATAGGTGAC‑A‑TAT‑AGCC‑GCACCAATAAAAATCCCCTCACTACCGCAAAt                                                         <  2:638656/138‑1 (MQ=255)

                                                                                           tGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTATTTCTAATTGGCTAAGTAGACGCA‑‑AT‑‑ACAA‑‑AATAGGTGAC‑A‑TAT‑AGCC‑GCACCAATAAAAATCCCCTCACTACCGCAAATAgag                                                     >  1:658523/1‑139 (MQ=255)

                                                                                           tGCTTATATTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCA‑‑AG‑‑ACAA‑‑AATAGGTGAC‑A‑TAT‑AGCC‑GCACCAATAAAAGTCCCCTCACTACCGCAAATAGTg                                                     >  1:487649/1‑141 (MQ=255)

                                                                                              ttATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCA‑‑AT‑‑ACAA‑‑AATAGGTGAC‑A‑TAT‑AGCC‑GCACCAATAAAAATCCCCTCACTACCGCAAACAGTGAgg                                                  >  1:49664/1‑141 (MQ=255)

                                                                                               tATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCA‑‑AT‑‑ACAA‑‑AATAGGTGAC‑A‑TAT‑AGCC‑GCACCAATAAAAATCCCCTCACTACCGCAAATAGTGAggg                                                 <  2:370522/141‑1 (MQ=255)

                                                                                                    ccgctCACATCATCGCTCTTGTCATGAGTGGCTGTGCCATTGCGTTTTTTTCGTCTGTGCTAAGTAGACGCA‑‑AT‑‑ATAA‑‑AACAGGTGGC‑A‑TAT‑AGCC‑TCACCAATAAAAATCCCCTCACTACCGCAAATAGTGAGGGGATTg                                            <  2:213888/137‑1 (MQ=255)

                                                                                                     cGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCA‑‑AT‑‑ACAA‑‑AATAGGTGAC‑A‑TAT‑AGCC‑GCACCAATAAAAATCCCCTCACTACCGCAAATAGTGAGGGGATTgg                                           >  1:237177/1‑141 (MQ=255)

                                                                                                     cGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACCCA‑‑AA‑‑ACAA‑‑AATCGGTGAC‑A‑TAT‑AGCC‑GCACCAATAAAAATCCCCTCACTACCGCAAATAGTGAAGGGATTgg                                           >  1:626273/1‑141 (MQ=255)

                                                                                                       ttCACATCATAGCACCAGTCATCAGTGGCGGTGCGATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCA‑‑AT‑‑ACAA‑‑AATAGGGGAC‑A‑TAC‑AGCC‑GCACCAATAAAAATCCCCTCACTACCGCAAATAGTGAGGGGCTTGgtg                                         <  2:268565/141‑1 (MQ=255)

                                                                                                               atAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCAACAA‑‑ATAATTAATAGGCGACTT‑TATAAGCCAGCATCGA‑AAAAATCCCCACACTACTGC‑AATAGTGAGGGGATTGGTGcat                                      >  1:484282/1‑141 (MQ=255)

                                                                                                                tAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCA‑‑ACAAATAATTAATAGGCGAC‑TTTATAAGCCAGCATCGA‑AAAAATCCCCACACTACTGC‑AATAGTGTGGGGATTGGTGcatt                                     >  2:677055/1‑140 (MQ=255)

                                                                                                                  gCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCA‑‑AT‑‑ACAA‑‑GATAGGTGAC‑A‑TAT‑AGCC‑GCACCAATAAAAATCCCCTCACTACCGCAAATAGTGAGGGGATTGGTGTATAAGTAAAt                              >  1:423073/1‑141 (MQ=255)

                                                                                                                       aGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCA‑‑AT‑‑ACAA‑‑ACTAGGTGAC‑A‑TAT‑AGCC‑GCACCAATAAAAATCCCCTCACTACCGCAAATAGTGAGGGGCTTGGTGTCTAAGTAAATACTTa                         >  2:118788/1‑141 (MQ=255)

                                                                                                                       aGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCA‑‑AT‑‑ACAA‑‑AATAGGTGAC‑A‑TAT‑AGCC‑GCACCAATAAAAATCCCCTCACTACCGCAAATAGTGAGGGGATTGGTGTCTAAGTAAATACTTa                         >  2:118702/1‑141 (MQ=255)

                                                                                                                         tcatcaGTGGCTGTGCCATTGCGTTTTTTTCTTCTTGGCTAAGTAGACGCA‑‑AT‑‑GCCA‑‑AATAGGTGAC‑A‑TAT‑AGCC‑GCACCAATAAAAATCCCCTCATTACCGCAAATAGTGAGGGGATTGTTGTATAAGTAAATACTTAtt                       <  2:423686/141‑1 (MQ=255)

                                                                                                                         gcatcaGTGGCTGTGCCATTGCGTTTTTTGCTTATTGGCTAAGTAGACGCA‑‑AT‑‑ACAA‑‑AATAGGTGAC‑A‑TAT‑AGCC‑GCACCAATAAAAATCCCCTCACTACCGCAAATAGTGAGGGGATTGGTGTATAAGTAAATACTTAtt                       <  1:61050/140‑1 (MQ=255)

                                                                                                                          catcaGTGGCTGTGCCATTGCGTTTTTTTTTTTTTTTCTAAGTAGACGCA‑‑AT‑‑ACAA‑‑AATAGGTGAC‑A‑TAT‑AGCC‑GCACCAATAAAAATCCCCTCACTACCGCAAATAGTGAGGGGATTGGTGTATAAGTAAATACTTAttt                      >  2:302004/1‑141 (MQ=255)

                                                                                                                               gTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCA‑‑AT‑‑ACAA‑‑AATAGGTGAC‑A‑TAT‑AGCC‑GCACCAATAAAAATCCCCTCACTACCGCAAATAGTGAGGGGATTGGTGTATAAGTAAATACTTATTTTCGtt                 <  1:452381/141‑1 (MQ=255)

                                                                                                                               gTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCA‑‑AT‑‑ACAA‑‑AATAGGTGAC‑A‑TAT‑AGCC‑GCACCAATAAAAATCCCCTCACTACCGCAAATAGTGAGGGGATTGGTGTATAAGTAAATACTTATTTTCGtt                 <  1:724184/141‑1 (MQ=255)

                                                                                                                               gTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCA‑‑AT‑‑ACAA‑‑AAAAGGTGAC‑A‑TAT‑AGCC‑GCACCAATAAAAATCCCCTCACTACCGCAAATAGTGAGGGGATTGGTGTATAAGTAACTACTTATTTTCGtt                 >  2:627244/1‑141 (MQ=255)

                                                                                                                                      tgCCATTGCGTTTTTTTCTAATTTGCTAAGTAGACGCA‑‑AT‑‑ACAA‑‑AATAGGTGAC‑A‑TAT‑AGCT‑GCACCAATAAAAATCCCCTCACTACCGCAAATAGTGAGGGGATTGGTGTATGCGTAAATCCTTATTTTGGTTGTCTTaa          >  1:409666/1‑141 (MQ=38)

                                                                                                                                              cGTTGTTTTCTTATTGGATAATTAGACGCA‑‑AT‑‑ACAA‑‑AATAGGTGAC‑A‑TAT‑AGCC‑GCATAAATAAACATCCCCTCACTACCGGAAATAGTGAGGGGATTGGTGTATAAGTAAATACTTATTTTCGTTGTCTTAATTATACTg  <  2:392736/141‑1 (MQ=255)

GCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTG‑TA‑TAA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCA‑‑AT‑‑ACAA‑‑AATAGGTGAC‑A‑TAT‑AGCC‑GCACCAATAAAAATCCCCTCACTACCGCAAATAGTGAGGGGATTGGTGTATAAGTAAATACTTATTTTCGTTGTCTTAATTATACTG  >  CP000730/1929785‑1930062

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`