BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP000730	2,728,368	A→G	4.7%	M124V (ATG→GTG)	pyrD →	dihydroorotate oxidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	2,728,368	0	A	G	4.7%	238.0 / 3.3	88	M124V (ATG→GTG)	pyrD	dihydroorotate oxidase
Reads supporting (aligned to +/- strand): ref base A (32/50); new base G (2/2); total (35/53)
Fisher's exact test for biased strand distribution p-value = 6.46e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.69e-01

TTGGAACACCTTGGATTCGGTGCTTTAGAATTAGGTGGTATCACACCTAAACCTCAACCGGGTAACCCTCAACCACGCATGTTTAGATTATTAGAAGATGACGCCTTGATAAATCGAATGGGCTTCAATAATATTGGTATGAACAAAGCACTAAGTCATTTGCGTAAAAATGCTTATCAAGTACCTGTTGGTATCAATGTTGGTGTGAATAAAATGACACCTTATGAAGCGCGTTATCAAGATTATATAAAGGTTATTGATACGTTTAAACACGACGTT  >  CP000730/2728230‑2728508

ttGGAACACCTTGGATTCGGTGCTTTAGAATTAGGTGGTATCACACCTAAACCTCAACCGGGTAACCCTCAACCACGCATGTTTAGATTATTAGAAGATGACGCCTTGATAAATCGAATGGGCTTCAATAATATTGGTATg                                                                                                                                            >  2:301469/1‑141 (MQ=255)

ttGGAAAACCTTGGATTCCGTGCTTTAGAATTAGGTGGTATCACACCTAAACCTCAACCGGGTAACCCTCAACCACGCATGTTTAGATTATTAGAAGATGACGCCTTGATAAATCGAATGGGCTTCAATAATATTGGTATg                                                                                                                                            <  1:701073/141‑1 (MQ=255)

  ggAACACCTTGGATTCGGTGCTTTAGCATTAGGTGGTATCACACGTAGACCTCAACGGGGTAACCCTCAACCACGCATGTTTAGATTATTCGAAGATGACGCCTTGATAAATCGAATGGGGTTCAATAATATTGGTATGaa                                                                                                                                          >  2:115205/1‑141 (MQ=255)

    aaCACCTTGGATTCGGTGCTTTAGAATTAGGTGGTATCACACCTAAACCTCAACCGGGTAACCCTCAACCACGCATGTTTAGATTATTAGAAGATGACGCCTTGATAAATCGAATGGGCTTCAATAATCTTGGTATCAAct                                                                                                                                        >  2:190011/1‑140 (MQ=255)

     acacCTTGGATTCGGTGCTTTAGAATTAGGTGGTATCACACCTAAACCTCAACCGGGTAACCCTCAACCACGCATGTTTAGATTATTAGAAGATGACGCCTTGATAAATCGAATGGGCTTCAATAATATTGGTATGAACaa                                                                                                                                       >  2:696913/1‑141 (MQ=255)

        ccTTGGATTCGGTGCTTTAGAATTAGGTGGTATCACACCTAAACCTCAACCGGGTAACCCTCAACCACGCATGTTTAGATTATTAGAAGATGACGCCTTGATAAATCGAATGGGCTTCAATAATATTGGTATGAACAAAGc                                                                                                                                    <  2:71516/141‑1 (MQ=255)

           tcgtgtCGGTCCTTTAGGATTATGTGGTATCACACCTAAACCTCAACCGGGTAACCCTCAACCACGCATGTTTAGATTATTAGAAGATGACGCCTTGATAAATCGAATGGGCTTCAATAATATTGGTGTGAACAAAGCACt                                                                                                                                 >  2:648480/6‑141 (MQ=255)

           tGGATTCGGTGCTTTAGAATTAGGTGGTATCACACCTAAACCTCAACCGGGTAACCCTCAACCACGCATGTTTAGATTATTAGAAGATGACGCCTTGATAAATCGAATGGGCTTCAATAATATTGGTGTGAACAAAGCACt                                                                                                                                 >  2:654097/1‑141 (MQ=255)

            ggATTCGGTGCTTTAAAATAAGGTGGTATCACACCTAAACCTCAACCGGGTAACCCTCAACCACTCATGTTTAGATTATTAGAAGATGACGCCTTGATAAATCGAATGGGCTTCAATAATATTGGTATGAACAAAGCACTa                                                                                                                                >  1:577134/1‑141 (MQ=255)

              agtctgTGCTTTAGAATTAGGTGGTATCACACCTAAACCTCAACCGGGTAACCCTCAACCACGCATGTTTAGATTATTAGAAGATGACGCCTTGATAAATCGAATGGGCTTCAATAATATTGGTATGAACAAAGCACTAAg                                                                                                                              <  2:705083/136‑1 (MQ=255)

                 ttgcgCTTTAGAATTAGGTGGTATCACACCTAAACCTCAACCGGGTAACCCTCAACCACGCATGTTTAGATTATTAGAAGATGACGCCTTGATAAATCGAATGGGCTTCAATAATATTGGTATGAACAAAGCACTAAGTCa                                                                                                                           <  2:775064/137‑1 (MQ=255)

                 cgttGCTTTAGAATTAGGTGGTATCACACCTAAACCTCAACCGGGTAACCCTCAACCACGCATGTTTAGATTATTAGAAGATGACGCCTTGATAAATCGAATGGGCTTCAATAATATTGGTATGAACAAAGCACTAAGTCa                                                                                                                           <  2:315824/138‑1 (MQ=255)

                  ggTGCTTTAGAATTAGGTGGTATCACACCTAAACCTCAACCGGGTAACCCTCAACCACGCATGTTTAGATTATTAGAAGATGACGCCTTGATAAATCGAATGGGCTTCAATAATATTGGTATGAACAAAGCACTAAGTCAt                                                                                                                          <  1:248246/141‑1 (MQ=255)

                        ttAGAATTAGGTGGTATCACACCTAAACCTCAACCGGGTAACCCTCAACCACGCATGTTTAGATTATTAGAAGATGACGCCTTGATAAATCGAATGGGCTTCAATAATATTGGTATGAACAAAGCACTAAGTCATTTGCGt                                                                                                                    <  1:509858/141‑1 (MQ=255)

                        ttAGAATTAGGTGGTATCACACCTAAACCTCAACCGGGTAACCCTCAACCACGCATGTTTAGATTATTAGAAGATGACGCCTTGATAAATCGAATGGGCTTCAATAATATTGGTATGAACAAAGCACTAAGTCATTTGCGt                                                                                                                    <  1:510786/141‑1 (MQ=255)

                        ttAGAATTAGGTGGTATCACACCTAAACCTCAACCGGGTAACCCTCAACCACGCATGTTTAGATTATTAGAAGATGACGCCTTGATAAATCGAATGGGCTTCAATAATATTGGTATGAACAAAGCACTAAGTCATTTGCGt                                                                                                                    >  1:349824/1‑141 (MQ=255)

                           gAATTAGGTGGTATCACACCTAAACCTCAACCGGGTAACCCTCAACCACGCATGTTTAGATTATTAGAAGATGACGCCTTGATAAATCGAATGGGCTTCAATAATATTGGTATGAACAAAGCACTAAGTCATTTGCGTaaa                                                                                                                 >  2:746101/1‑141 (MQ=255)

                                aGGTGGTATCACACGTAAACCTCAACCGGGTAACCCTCAACCACGCATGTTTAGATTATTAGAAGATGACGCCTTGATAAATCGAATGGGCTTCAATAATATTGGTATCAACAAAGCACTAAGTCATTTGCGTAAACATGc                                                                                                            <  1:553325/141‑1 (MQ=255)

                                 ggtggtATCACACCTAAACCTCAACCGGGTAACCCTCAACCACGCATGTTTAGATTATTAGAAGATGACGCCTTGATAAATCGAATGGGCTTCAATAATATTGGTATGAACAAAGCACTAAGTCATTTGCGTAAAAATGCt                                                                                                           <  2:135989/141‑1 (MQ=255)

                                  gtggtATCACACCTAAACCTCAACCGGGTAACCCTCAACCACGCATGTTTAGATTATTAGAAGATGACGCCTTGATAAATCGAATGGGCTTCAATAATATTGGTATGAACAAAGCACTAAGTCATTTGCGTAAAAATGCtt                                                                                                          >  1:741657/1‑141 (MQ=255)

                                  gtggtACCACACCCAAACCTCAACTCGGTAACCCTCAACCACGCATGTTTAGATTATTAGAAGATGACGCCTTGATAAATCGAATGGGCTTCAATAATATTGGTATGAACAAAGCACTAAGTCATTTGCGTAAAAATGCtt                                                                                                          <  2:109664/141‑1 (MQ=255)

                                    ggtATCACACCTAAATCTCATCCGGGTAACCCTCAACCACGCATGTTTAGATGATTAGACGATGACGCCTTGATAAATCGAATGGGCTTCAATAATATTGGTATGAACAAAGCACTAAGTTATTTGCGTAAAAGTGCTTAt                                                                                                        <  1:484861/141‑1 (MQ=255)

                                        tCACACCTAAACCTCAACCGGGTAACCCTCACCACCGCATGTTTAGATTATTAAAAAATAACGCCTTTATAAATCGAATTGTCTTCAATAATAGTGGTATGAACAAAGCACTAAGTCATTTGCGTAAAAATGCTTATCAAg                                                                                                    <  2:644758/141‑1 (MQ=255)

                                        tCACACCTAAACCTCAACCGGGTAACCCTCAACCACGCATGTTTAGATTATTAGAAGATGACGCCTTGATAAATCGAATGGGCTTCAATAATATTGGTATGAACAAAGCACTAAGTCATTTGCGTAAAAATGCTTATCAAg                                                                                                    <  1:460370/141‑1 (MQ=255)

                                         cacaCCTAAACCTCAACCGGGTAACCCTCAACCACGCATGTTTAGATTATTAGAAGATGACGCCTTGATAAATCGAATGGGCTTCAATAATATTGGTATGAACAAAGCACTAAGTCATTTGCGTAAAAATGCTTATCAAGt                                                                                                   <  1:204853/141‑1 (MQ=255)

                                            acCTAAACCTCAACCGGGTAACCCTCATCCACGCATGTTTAGATTATTAGAAGATGACGCCTTGATAAATCGAATGGGCTTCAATAATATTGGTATGAACAAATCACTAAGTCATTTGCGTAAACGTGCTTagggagcacg                                                                                                <  2:169207/141‑10 (MQ=255)

                                            acCTAAACCTCAACCGGGTAACCCTCAACCACGCATGTTTAGATTATTAGAAGATGACGCCTTGATAAATCGAATGGGCTTCAATAATATTGGTATGAACAAAGCACTAAGTCATTTGCGTAAAAATGCTTATCAAGTAcc                                                                                                <  1:350516/141‑1 (MQ=255)

                                            acCTAAACATCCACCGAGTTACACTCAACCACGACCGTCTCGATTATTAGAAGATGTCGCTTTGATAAATCGACTGGGCTTCAATAATATTGGTATGAACAAAGCACTAAGTCATTTGCGTAAAAATGCTTATCAAGTAcc                                                                                                <  1:159503/141‑1 (MQ=255)

                                             ccTAAACCTCAACCGGGTAACCCTCAACCACGCATGTTTACATTATTAGAAGATGACGCCTTGATAAATCGAATGGGCTTCAATAATATTGGTATGAACAAAGCACTAAGTCATTTGCGTAAAAATGCTTATCAAGTACCt                                                                                               <  1:771586/141‑1 (MQ=255)

                                              ccaacccTCAACCGGGCAACCCTCAACCACGCATGTTTATATTATTAGAAGATGACGCCTTGATAAATCGAATGGGCTTCAATAATATTGGTATGAACAAAGCACTAAGTCATTTGCGTAAAAATGCTTATCAAGTACCTg                                                                                              <  1:208275/136‑1 (MQ=255)

                                              cTAAACCTCAACCGGGTAACCCTCAACCACGCATGTTTAGATTATTAGAAGATGACGCCTTGATAAATCGAATGGGCTTCAATAATATTGGTATGAACAAAGCACTAAGTCATTTGCGTAAAAATGCTTATCAAGTACCTg                                                                                              <  1:578274/141‑1 (MQ=255)

                                              cTAAACCTCAACCGGGTAACCCTCAACCACGCATGTTTAGATTATTAGAAGATGACGCCTTGATAAATCGAATGGGCTTCAATAATATAGGGTGGAACAAAGCACTAAGTCATATGCGTAAAAATGCTTATCAAGTACCTg                                                                                              >  1:758155/1‑141 (MQ=255)

                                                   ccTCAACCGGGTAACCCTCAACCACGCATGTTTAGATTATTAGAAGATGACGCCTTGATAAATCGAATGGGCTTCAATAATATTGGTATGAACAAAGCACTAAGTCATTTGCGTAAAAATGCTTATCAAGTACCTGTTGGt                                                                                         >  1:571284/1‑141 (MQ=255)

                                                   ccTCAACCGGGTAACCCTCAACCACGCATGTTTAGATTATTAGAAGATGACGCCTTGATAAATCGAATGGGCTTCAATAATATTGGTATGAACAAAGCACTAAGTCATTTGCGTAAAAATGCTTATCAAGTACCTGTTGGt                                                                                         >  1:575665/1‑141 (MQ=255)

                                                    cTCAACCGGGTAACCCTCAACCACGCATGTTTAGATTATTAGAAGATGACGCCTTGATAAATCGAATGGGCTTCAATAATATTGGTATGAACAAAGCACTAAGTCATTTGCGTAAAAATGCTTATCAAGTACCTGTTGGTa                                                                                        <  1:687424/141‑1 (MQ=255)

                                                     tCAACCGGGTAACCCTCAACCACGCATGTTTAGATTATTAGAGGATGACGCCTTGATAAATCGAATGGGCTTCAATAATATTGGTATGAACAAAGCACTAAGTCATTTGCGTAAAAATGCTTATCAAGTACCTGTTGGTAt                                                                                       >  1:541530/1‑141 (MQ=255)

                                                      cAACCGGGTAACCCTCAACCACGCATGTTTAGATTATTAGAAGATGACGCCTTGATAAATCGAATGGGCTTCAATAATATTGGTATGAACAAAGCACTAAGTCCTTTGCGTAAAAATGCTTATCAAGTACCTGTTGGTATc                                                                                      >  1:759714/1‑141 (MQ=255)

                                                      cAACCGGGTAACCCTCAACCACGCATGTTTAGATTATTAGAAGATGACGCCTTGATAAATCGAATGGGCTTCAATAATATTGGTATGAACAAAGCACTAAGTCATTTGCGTAAAAATGCTTATCAAGTACCTGTTGGTATc                                                                                      <  2:247815/141‑1 (MQ=255)

                                                      cAACCGGGTAACCCTCAACCACGCATGTTTAGATGATTAGAAGATGACGCCGTCATAAATCGAATGGGCTTCAATAATATTTGTATGAACAAAGCACTAAGTCATTTGCGTAAAAATGCTTATCAAGTACCTGTTGGTATc                                                                                      <  2:601414/141‑1 (MQ=255)

                                                       aaCCGGGTAACCCTCAACCACGCATGTTTAGATTATTAGAAGATGACGCCTTGATAAATCGAATGGGCTTCAATAATATTGGTATGAACAAAGCAATAAGTCATTTGCGTAAAAATGCTTATCAAGTACATGTTGGTATCa                                                                                     >  2:750131/1‑141 (MQ=255)

                                                         ccGGGTAACCCTCAACCACGCATGTTTAGATTATTAGAAGATGACGCCTTGATAAATGGAATGGGATTCAATAATATTGGTATGAACAAAGCACTAAGTCATTTGCGCAAAAATGCTTATCAAGTACCTGTTGGTATCAAt                                                                                   >  2:167073/1‑141 (MQ=255)

                                                          cGGGTAACCCTCCACCACGCATGTTTAGCTTATTAGAAGATGACGCCTTGATAAATCGAATGGGCTTCAATAATATTGGTATGAACAAAGCACTAAGTCATTTGCGTAAAAATGCTTATCACGTACCTGTTGGTATCAGTg                                                                                  <  2:710887/141‑1 (MQ=255)

                                                            ggTAACCCTCAACCACGCATGTTTAGATTATTAGAAGATGACGCCTTGATACATCGAATGGGCTTCAATAATATTGGTATTAACAAAGCACTAAGTCATTTGCGTAAAAATGCTTATCAAGTACCTGTTTGTATCATTGtt                                                                                >  1:412002/1‑141 (MQ=255)

                                                            ggTAACCCTCAACCACGCATGTTTAGATTATTAGAAGATGACGCCTTGATAAATCGAATGGGCTTCAATAATATTGGTATGAACAAAGCACTAAGTCATTTGCGTAAAAATGCTTATCAAGTACCTGTTGGTATCAATGtt                                                                                >  1:287070/1‑141 (MQ=255)

                                                             gTAACCCTCAACCACGCATGTTTAGATTATTAGAAGATGACGCCTTGATAAATCGAATGGGCTTCAATAATATTGGTATGAACAAAGCACTAAGTCATTTGCGTAAAAATGCTTATCAAGTACCTGTTGGTATCAATGTTg                                                                               >  2:160877/1‑141 (MQ=255)

                                                                aCCCTCAACCACGCATGTTTAGATTATTAGAAGATGACGCCTTGATAAATCGAATGGGCTTCAATAATATTGGTATGAACAAAGCACTAAGTCATTTGCGTAAAAATGCTTATCAAGTACCTGTTGGTATCAATGTTGgtg                                                                            >  2:763602/1‑141 (MQ=255)

                                                                 cccTCAACCACGCATGTTTAGATTATTAGAAGATGACGCCTTGATAAATCGAATGGGCTTCAATAATATTGGTATGAACAAAGCACTAAGTCATTTGCGTAAAAATGCTTATCAAGTACCTGTTGGTATCAATGTTGgtgt                                                                           <  2:690413/141‑1 (MQ=255)

                                                                       aCCACGCATGTTTAGATTATTAGAAGATGACGCCTTGATAAATCGAATGGGCTTCAATAATATTGGTGTGAACAAAGCACTAAGTCATTTGCGTAAAAATGCTTATCAAGTACCTGTTGGTATCAATGTTGGTGTGAATaa                                                                     <  1:648480/141‑1 (MQ=255)

                                                                       aCCACGCATGTTTAGATTATTAGAAGATGACGCCTTGATAAATCGAATGGGCTTCAATAATATTGGTGTGAACAAAGCACTAAGTCATTTGCGTAAAAATGCTTATCAAGTACCTGTTGGTATCAATGTTGGTGTGAATaa                                                                     <  1:654097/141‑1 (MQ=255)

                                                                         ctcGCATGTTTAGAATAGTAGAAAATGCCGCCTTAATAAAAAGAATGGGCTTCAATAATATTGGTCTGAACAAAGCACTAAGTCATTTGCGTAAAAATGCTTATCAAGTCCCTGTTGGTATTAATGTTGGTGTGAATaaaa                                                                   <  2:722282/139‑1 (MQ=255)

                                                                         cACGCATGTTTAGATTATTAGAAGATGACGCCTTGATAAATCGAATGGGCTTCAATAATATTGGTATGAACAAAGCACTAAGTCATTTGCGTAAAAATGCTTATCAAGTACCTGTTGGTATCAATGTTGGTGTGAATaaaa                                                                   <  2:303523/141‑1 (MQ=255)

                                                                            gCATGTTTAGATTATTAGAAGATGACGCCTTGATAAATCGAATGGGCTTCAATAATATTGGTATGAACAAAGCACTAAGTCATTTGCGTAAAAATGCTTATCAAGTACCTGTTGGTATCAATGTTGGTGTGAATAAAATGa                                                                <  1:261977/141‑1 (MQ=255)

                                                                                gTTTAGATTATTAGAAGATGACGCCTTGATAAATCGAATGGGCTTCAATAATATTGGTATGAACAAAGCACTAAGTCATTTGCGTAAAAATGCTTATCAAGTACCTGTTGGTATCAATGTTGGTGTGAATAAAATGACAcc                                                            >  1:106835/1‑141 (MQ=255)

                                                                                 tttAGATTATTAGAAGATGACGCCTTGATAAATCGAATGGGCTTCAATAATATTGGTATGAACAAAGCACTAAGTCATTTGCGTAACAATGCTTATCAAGTACCTGTTGGGATCAATGTTGTAGTGAATGAAAttgcgctt                                                           >  2:729928/1‑134 (MQ=255)

                                                                                 tttAGATTATTAGAAGATGACGCCTTGATAAATCGAATGGGCTTCAATAATATTGGTATGAACAAAGCACTAAGTCATTTGCGTAAAAAAGCTAAACAAGTACCCGTTGGTATCAATGTTGGTGTGAATAAAATGACACCt                                                           >  2:223576/1‑141 (MQ=255)

                                                                                  ttAGATTATTAGAAGATGACGCCTTGATAAATCGAATGGGCTTCAATAATATTGGTATGAACAAAGCACTAAGTCATTTGCGTAAAAATGCTTATCAAGTACCTGTTGGTATCAATGTTGGTGTGAATAAAATGACACCtt                                                          <  1:749410/141‑1 (MQ=255)

                                                                                         attaGAAGATGACGCCTTGATAAATCGAATGGGCTTCAATAATATTGGTATGAACAAAGCACTAAGTCATTTGCGTAAAAATGCTTATCAAGTACCTGTTGGTATCAATGTTGGTGTGAATAAAATGACACCTTATGAAgc                                                   <  1:773790/141‑1 (MQ=255)

                                                                                          ttaGAAGATGACGCCTTGATAAATCGAATGGGATTAAATAATTGTGTGATGAACAAAGCACTAAGTCATTTGCGTAAAAATGCTTATCAAGTACCTGTTGGTATCAATGTTGGTGTGAATAAAATGACACCTTATGAAgcg                                                  <  1:142190/141‑1 (MQ=255)

                                                                                            agaagaTGACGCCTTGATAAATCGAATGGGCTTCAATAATATTGGTATGAACAAAGCACTAAGTCATTTGCGTAAAAATGCTTATCAAGTACCTGGTGGGATCAATGTTGGTGTGAATAAAATGACACCTTATGAAGcgcg                                                <  1:217498/141‑1 (MQ=255)

                                                                                             gaagaTGACGCCTTGATAAATCGAATGGGCTTCAATAATATTGGTATGAACAAAGCACTAAGTCATTTGCGTAAAAATTCTTATCAAGTACCTGTTGGTATCAATGTTGGTGTGAATAAAATGACACCTTATGAAGCGCGt                                               <  2:609803/141‑1 (MQ=255)

                                                                                             gaagaTGACGCCTTGATAAATCGAATGGGCTTCAATAATATTGGTATGAACAAAGCACTAAGTCATTTGCGTAAAAATGCTTATCAAGTACCTGTTGGTATCAATGTTGGTGTGAATAAAATGACACCTTATGAAGCGCGt                                               <  2:31058/141‑1 (MQ=255)

                                                                                              aagaTGACGCCTTGATAAATCGAATGGGCTTCAATAATATTGGTATGAACAAAGCACTAAGTCATTTGCGTAAAAATGCTTATCAAGTACCTGTTGGTATCAATGTTGGTGTGAATAAAATGACACCTTATGAAGCGCGtt                                              >  1:329332/1‑141 (MQ=255)

                                                                                                gaTGACGCCTTGATAAATCGAATGGGCTTCAATAATATTGGTATGAACAAAGCACTAAGTCATTTGCGTAAAAATGCTTATCAAGTACCTGTTGGTATCAATGTTGGTGTGAATAAAATGACACCTTATGAAGCGCGTTAt                                            <  1:752116/141‑1 (MQ=255)

                                                                                                  tGACGCCTTGATAAATCGAATGGGCTTCAATAATATTGGTATGAACAAAGCACTAAGTCATTTGCGTAAAAATGCTTATCAAGTACCTGTTGGTATCAATGTTGGTGTGAATAAAATGACACCTTATGAAGCGCGTTATCa                                          <  2:753567/141‑1 (MQ=255)

                                                                                                      gCCTTGATAAATCGAATGGGCTTCAATAATATTGGTATGAACAAAGCACTAAGTCATTTGCGTAAAAATGCTTATCAAGTACCTGTTGGTATCAATGTTGGTGTGACTAAAATTACACCTTATGAAGCGCGTTATCAAGAt                                      <  1:767485/141‑1 (MQ=255)

                                                                                                      gCCTTGATAAATCGAATGGGCTTCAATAATATTGGTATGAACAAAGCACTAAGTCATTTGCGTAAAAATGCTTATCAAGTACCTGTTGGTATCAATGTTGGTGTGAATACAATGACCCCTCATGAAGCCCGTTATGAAGAt                                      >  2:242196/1‑141 (MQ=255)

                                                                                                         ttGATAAATCGAATGGGCTTCAATAATATTGGTATGAACAAAGCACTAAGTCATTTGCGTAAAAATGCTTATCAAGTACCTGTTGGTATCAATGTTGGTGTGAATAAAATGACACCTTATGAAGCGCGTTAGCAAGATtat                                   >  2:418654/1‑141 (MQ=255)

                                                                                                          tGATAAATCGAATGGGCTTCAATAATATTGGTATGAACAAAGCACTAAGTCATTTGCGTAAAAATGCTTATCAAGTACCTGTTGGTATCAATGTTGGTGTGAATAAAATGACACCTTATGAAGCGCGTTATCAAGATtata                                  <  2:201756/141‑1 (MQ=255)

                                                                                                          tGATAAATCGAATGGGCTTCAATAATATTGGTATGAACAAAGCACTAAGTCATTTGCGTAAAAATGCTTATCAAGTACCTGTTGGTATCAATGTTGGTGTGAATAAAATGACACCTTATGAAGCGCGTTATCAAGATtata                                  <  1:721614/141‑1 (MQ=255)

                                                                                                           gATAAATCGAATGGGCTTCAATAATATCGGTATGAACAAAGCACTAAGTCATTTGCGTAAAAATGCTTATCAAGTACCTGTTGGTATCAATGTTGGTGTGAATAAAATGACACCTTATGAAGCGCGTTATCAAGATtatat                                 <  1:210265/141‑1 (MQ=255)

                                                                                                            aTAAATCGAATGGGCTTCAATAATATTGGTATGAACAAAGCACTAAGTCATTTGCGTAAAAATGCTTATCAAGTACCTGTTGGTATCAATGTTGGTGTGAATAAAATGACACCTTATGAAGCGCGTTATCAAGATtatata                                >  2:66291/1‑141 (MQ=255)

                                                                                                              gaagcGAATGGGCTGCAATGATATTGGTATGAACAAAGCACTAAGTGATTTGCGTAAAAATGCTTATCAAGTACCTGTTGGTATCAATGTTGGTGTGAATAAAAGGACACCTTATGACGCGCGTTATCAAGATTATATaaa                              <  2:23248/137‑1 (MQ=255)

                                                                                                              aaaTCGAATGGGCTTCAATAATATTGGTATGAACAAAGCACTAAGTCATTTGCGTAAAAATGCTTATCAAGTACCTGTTGGTATCAATGTTGGTGTGAATAAAATGACACCTTATGAAGCGCGTTATCAAGATTATATaaa                              <  2:264094/141‑1 (MQ=255)

                                                                                                                aTCGAATGGGCTTTAATAATATTGGTATGAACAAAGCACTAAGTCATTTGCGTAAAAATGCTTATCAAGTACCTGTTGGTATCAATGTTGGTGTGAATAAAATGACACCTTATGAAGCGCGTTATCAAGATTATATAAAgg                            <  1:611974/141‑1 (MQ=255)

                                                                                                                 tCGAATGGGCTTCAATAATATTGGTATGAACAAAGCACTAAGTCATTTGCGTAAAAATGCTTATCAAGTACCTGGTGGTGTCAATGTTGGTGGTAATAAATTGACACCGTATGAAGCGCGTTATCAAGAtgctcaaaaggt                           >  1:293824/1‑130 (MQ=255)

                                                                                                                    aaTGGGCTTCAATAATATTGGTATGAACAAAGCAGTAAGTCATTTGCTTAAAAATGCTTATCAAGTACCTGTTGGTATCAATGTTGGTGTGAATAAAATGACACCTTATGAAGCGCGTTATCAAGATTATATAAAGGTTAt                        <  1:142973/141‑1 (MQ=255)

                                                                                                                     aTGGGCTTCAATAATATTGGTATGAACAAAGCACTAAGCCATTTGCGTAAAAATGAGTATCAAGTACCTGTTGGTATCAATGTTGGCGTGAATAAAGTGACACCTTATGAAGCGCGTTCTCAAGCTTATCTCAAGGTTCtt                       >  2:595035/1‑141 (MQ=255)

                                                                                                                         gcctCAATAATATGGGTATGAACAAAGCACTAAGTCATTTGCGTAAAAATGCTTATCAAGTACCTGTTGGTATCAATGTTGGGGTGAACGAAAGGGCTCCTTATTAAGCTCGTTATCAAGATTATATAAAGGTTATTGATa                   <  1:181762/138‑1 (MQ=255)

                                                                                                                          cTTCAATAATATTGGTATGAACAAAGCACTAAGTCATTTGCGTAAAAATGCTTATCAAGTACCTGTTGGTATCAATGTTGGTGTGAATAAAATGACACCTTATGAAGCGCGTTATCAAGATTATATAAAGGTTATTGATAc                  >  2:766482/1‑141 (MQ=255)

                                                                                                                             ccataataGTGGTATGAACAAAGCACTAAGTCATTTGGCTAAAAATCCTTATCAAGTACCTTTTTGTATCAATTTTCGTGTTAATAAAATGACACCTTATGAAGCCCGTTATCAAGATTATATAAATTTTATTGATACttt               <  2:600498/139‑1 (MQ=255)

                                                                                                                              aataatATTGGTATGAACAAAGCACTAAGTCATTTGCGTAAAAATGCTTATCAAGTACCTGTTGGTATCAATGTTGGTGTGAATAAAATGACACCTTATGAAGCGCGTTATCAAGATTATATAAAGGTTATCGATACGttt              <  2:496901/141‑1 (MQ=255)

                                                                                                                                taataTTGGTATGAACAAAGCACTAAGTCATTTGCGTAAAAATGCTTATCAAGTACCTGTTGGTATCAATGTTGGTGTGAATAAAATGACACCTTATGAAGCGCGTTATCAAGATTATATAAAGGTTATTGATACGTTTaa            <  2:326409/141‑1 (MQ=255)

                                                                                                                                taataTTGGTATGAACAAAGCACTAAGTCATTTGCGTAAAAATGCTTATCAAGTACCTGTTGGTATCAATGTTGGTGTGAATAAAACGACACCTTATGAAGCGCGTTATCAAGATTATATAAAGGTTATTGATACGTTTaa            >  1:682272/1‑141 (MQ=255)

                                                                                                                                 aataTTGGTATGAACAAAGCACTGAGTCATTTGCGTAAAAATGCTTATCAAGTACCTGTTGGTATCAATGTTGGTGTGAAGAAAATGACACCTTATGAAGCGCGTTATCAAGATTATATAAAGGTTATTGATACGTTTaaa           >  2:554219/1‑141 (MQ=255)

                                                                                                                                  atatTGGTATGAACAAAGCACTAAGTCATTTGCGTAAAAATGCTTATCAAGTACCTGTTGGTATCAATGTTGGTGTGAATAAAATGACACCTTATGAAGCGCGTTATCAAGATTATATAAAGGTTATTGATACGTTTAAac          <  1:227560/141‑1 (MQ=255)

                                                                                                                                  atatTGGTATGAACAAAGCACTAAGTCATTTGCGTAAAAATGCTTATCAAGTACCTGTTGGTATCAATGTTGGTGTGAATAAAATGACACCTTATGAAGCGCGTTATCAAGATTATATAAAGGTTATTGATACGTTTAAac          >  2:339053/1‑141 (MQ=255)

                                                                                                                                      tGGTATGAACAAAGCACTAAGTCATTTGCGTAAAAATGCTTATCAAGTACCTGTTGGTATCAATGTTGGTGTGAATAAAATGACACCTTATGAAGCGCGTTATCAAGATTATATAAAGGTTATTGATACGTTTAAACacga      >  1:551838/1‑141 (MQ=255)

                                                                                                                                         tATGAACAAAGCCCTAAGTCATTTGCGTAAAAATGCTTATCAAGTACCTGTTGGTATCAATGTTGGTGTGAATAAAATGACACCTTATGAAGCGCGTTATCAAGATTATATAAAGGTTATTGATACGTTTAAACACGACGt   <  1:352812/141‑1 (MQ=255)

                                                                                                                                          aTGAACAAAGCACTTAGTCATTTGCTTAAAAATGCTTATCAAGTACCTGTTGGTATCAATGTTGGGGTGAATAAAATGACACCTTATGAAGCGCGTTATCAAGATTATATAAAGGTTATTGATACGTTTAAACACGACGtt  <  2:723683/141‑1 (MQ=255)

TTGGAACACCTTGGATTCGGTGCTTTAGAATTAGGTGGTATCACACCTAAACCTCAACCGGGTAACCCTCAACCACGCATGTTTAGATTATTAGAAGATGACGCCTTGATAAATCGAATGGGCTTCAATAATATTGGTATGAACAAAGCACTAAGTCATTTGCGTAAAAATGCTTATCAAGTACCTGTTGGTATCAATGTTGGTGTGAATAAAATGACACCTTATGAAGCGCGTTATCAAGATTATATAAAGGTTATTGATACGTTTAAACACGACGTT  >  CP000730/2728230‑2728508

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`