Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 973,213 C→A 100% E175* (GAG→TAG)  USA300HOU_0930 ← possible HAD superfamily hydrolase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP000730973,2130CA100.0% 227.9 / ‑6.5 73E175* (GAG→TAG) USA300HOU_0930possible HAD superfamily hydrolase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base A (49/22);  minor base T (1/1);  total (50/23)
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

CTTCATCACCGAATGCAATAATATTATTTCGGTCAATATTTAAAAATTGTCTAACTTGCTCAATGCCTCTTGCTTTATTAATACCAAGTTTTACAATTTCAATGACAGGGAATGGTGCGCCCCAGCGTCGATGCTCAATATGATCGGCATAAAAATGAGTAAGCATATTTTTGATTTCAGGTATTTTACTTTCTTCGGCTTCAATTAAAATTGAGGTAGGGGATTCTTTCAAGTGGACAAGTAAATTACCAGTTTGAATTCTTGGATTACCC  >  CP000730/973078‑973349
                                                                                                                                       |                                                                                                                                        
cTTCATCACCGAATGCAATAATATTATTTCGGTCAATATTTAAAAATTGTCTAACTTGCTCAATGCCTCTTGCTTTATTAATACCAAGTTTTACAATTTCAATGACAGGGAATGGTGCGCCCCAGCGTCGATGCTAAatat                                                                                                                                     >  2:340409/1‑141 (MQ=255)
 ttCATCACCGAATGCAATAATATTATTTCGGTCAATATTTAAAAATTGTCTAACTTGCTCAATGCCTCTTGCTTTATTAATACCAAGTTTTACAATTTCAATGACAGGGAATGGTGCGCCCCAGCGTCGATGCTAAATATg                                                                                                                                    >  1:33058/1‑141 (MQ=255)
 ttCATCACCGAATGCAATAATATTATTTCGGTCAATATTTAAAAATTGTATAACTTGCTCAATGCCTCTTGATTTATTAATACCAAGTTTTACAATTTCAATGACAGGGAATGGTGCGCCCCAGCGTCGATGCTAAATATg                                                                                                                                    >  2:409451/1‑141 (MQ=255)
  tcatcaCCGAATGCAATAATATTATTTCGGTCAATATTTAAAAATTGTCTAACTTGCTCAATGCCTCTTGCTTTATTAATACCCCGTTTTCCAATTTCAATGACAGGGCATGGTGCGCCCCAGCGTCGATGCTAAATATGa                                                                                                                                   >  2:518930/1‑141 (MQ=255)
  tcagaaCCGAATGCAATAATATTGTTTCGGTCAATATTTAAAAATTGACTAACATGCTCAATGCCTCCTTCTTTATTAATACCAAATTTTACAATTTCAATGCCAGGGAATGGTGCGCCCCAGCGGCGGTGCTAAATGTGa                                                                                                                                   >  2:295419/6‑141 (MQ=255)
   catcaCCGAATGCAATAATATTATTTCGGTCAATATTTAAAAATTGTCTAACTTGCTCAATGCCTCTTGCTTTATTAATACCAAGTTTTACAATTTCAATGACAGGGAATGGTGCGCCCCAGCGTCGATGCAAAATATGAt                                                                                                                                  >  2:71663/1‑141 (MQ=255)
      caCCGAATGCAATAATATTATTTCGGTCAATATTTAAAAATTGTCTAACTTGCTCAATGCCTCTTGCTTTATTAATACCAAGTTTTACAATTTCAATGACAGGGTATGGGGCGCCCCAGCGTCGATGCTAAATATGATCgg                                                                                                                               >  1:299723/1‑141 (MQ=255)
            aTGCAATAATATTATTTCGGTCAATATTTAAAAATTGTCTAACTTGCTCAATGCCTCTTGCTTTATTAATACCAAGTTTTACAATTTCAATGACAGGGAATGGTGCGCCCCAGCGTCGATGCTAAATATGATCGGCATaaa                                                                                                                         <  2:30206/141‑1 (MQ=255)
               cAATAATATTATTTCGGTACATATTTAAAAATTGTCTAACTTGCTCAATGCCTCTTGCTTTATTAATACCAAGTTTTACAATTTCAATGACAGGGAATGGTGCGCCCCAGCGTCGATGCTAAATATGATCGGCATAAAAAt                                                                                                                      <  2:341736/141‑1 (MQ=255)
                      attattTCGGTCAATATTTAAAAATTGTCTAACTTGCTCAATGCCTCTTGCTTTATTAATACGAAGTTTTACAATTTCAATGACAGGGAATGGTGCGCCCCAGCGTCGATGCTAACTATGATCGGCATAAAAATGAGTAAg                                                                                                               >  2:329435/1‑141 (MQ=255)
                       ttattTCGGTCAATATTTAAAAATTGTCTAACTTGCTCAATGCCTCTTGCTTTATTAATACCAAGTTTTACAATTTCAATGACAGGGAATGGTGCGCCCCAGCGTCGATGCTAAATATGATCGGCATAAAAATGAGTAAGc                                                                                                              <  1:671159/141‑1 (MQ=255)
                         attTCGGTCAATATTTAAAAATTGTCTAACTTGCTCAATGCCTCTTGCTTTATTAATACCAAGTTTTACAATTTCAATGACAGGGAATGGTGCGCCCCAGCGTCGATGCTAAATATGATCGGCATAAAAATGAGTAAGCat                                                                                                            >  1:362668/1‑141 (MQ=255)
                            tCGGTCAATATTTAAAAATTGTCTAACTTGCTCAATGCCTCTTGCTTTATTAATACCAAGTTTTACAATTTCAATGACAGGGAATGGTGCGCCCCAGCGTCGATGATAAATATGATCGGCATAAAAATGAGTAAGCatagt                                                                                                         >  2:549887/1‑139 (MQ=255)
                              ggTCAATATTTAAAAACTGTCTAACTTGCTCAATGCCTCTTGCTTTATTAATACCAAGTTTTACGATTTCAATGACAGGGAATGGTGCGCCCCAGCGTCGATGCTAAATATGATCGGCATAAAAATGAGTAAGCATAtttt                                                                                                       <  1:92264/141‑1 (MQ=255)
                                   ctatTTAAAAATTGTCTCACTTGCCCAATGCCTCCTGCTTTATTAATACCCAGTTTTACAATTTCAATGACAGGGAATGGTCCGCCCCAGCGTCGATGCTAAATATGCACCGAACAGATATGAGTAAGAATCTTTTTGAtt                                                                                                  >  2:142172/2‑141 (MQ=255)
                                   atatTTAAAAATTGTCTAACTTGCTCAATGCCTCTTGCTGTATTAATACCAAGTTTTACAATTTCAATTACAGGGAATGGTGCGCCCCAGCGTCGAAGCTAAATATGATCGGCAGAAAAATGAGTAAGCATTATTTTGCtt                                                                                                  >  1:88319/1‑141 (MQ=255)
                                   atatTTAAAAATTGACTAACTTGCTCAATGCCTCTTGCTTTATTAATACCAAGTTTTACAATTTCAATGACAGGGAATGGTGCGCCCCAGCGTCGATGCTAAATATGATCGGCATAAAAATGAGTAAGCATATTTTTGAtt                                                                                                  >  1:364003/1‑141 (MQ=255)
                                        tAAAAATTGTCTAACTTGCTCAATGCCTCTTGCCTTATTAATACCAAGTTTTACAATTTCAATGACAGGGAATGGTGCGCCCCAGCGTCGATGCTAAATATGATCGGCATAAAAATGAGTAAGCATATTTTTGATTACAgg                                                                                             <  1:472092/141‑1 (MQ=255)
                                               tGTCTAACTTGCTCAATGCCTCTTGCTTTATTAATACCAAGTTTTACAATTTCAATGACAGGGAATGGTGCGCCCCAGCGTCGATGCTAAATATGATCGGCATAAAAATGAGTAAGCATATTTTTGATTTCAGGTATTTTa                                                                                      >  1:121859/1‑141 (MQ=255)
                                                            cAATGCCTCCATTTTAATTAATACCAAGTTTTACAATTTCAATGACAGGGAATGGTGCGCCCCAGCGTCGATGCTAAATATGATCGGCATAAAAATGAGTAAGCATATTTTTGATTTCAGGTATTTTACTTTCTTCGGCtt                                                                         >  2:323737/1‑141 (MQ=255)
                                                              aTGCCTCTTGCTTTATTAATACCAAGTTTTACAATTTCAATGACAGGGAATGGTGCGCCCCAGCGTCGATGCTAAATATGATCGGCATAAAAATGAGTAAGCATATTTTTGATTTCAGGTATTTTACTTTCTTCGGCTTCa                                                                       >  2:264022/1‑141 (MQ=255)
                                                              aTGCCTCTTGCTTTATTAATACCAAGTTTTACAATTTCAATGACAGGGAATGGTGAGCCCCAGCGTCGATGCTAAATATGATCGGCATAAAAATGAGTAAGCATATTTTTGATTTCAGGTATTTTACTTTCTTCGGCTTCa                                                                       >  1:559283/1‑141 (MQ=255)
                                                                 ccTCTTGCTTTATTAATACCAAGTTTTACAATTTCAATGACAGGGAATGGTGCGCCCCAGCGTCGATGCTAAATATGATCGGCATAAAAATGAGTAAGCATATTTTTGATTTCAGGTATTTTACTTTCTTCGGCTTCAAtt                                                                    <  1:316869/141‑1 (MQ=255)
                                                                      tGCTTTATTAATACCAAGTTTTACAATTTCAATGACAGGGAATGGTGCGCCCCAGCGTCGATGCTAAATATGATCGGCATAAAAATGAGTAAGCATATTTTTGATTTCGGGTATTTTGAGTTCTGCGGCTTCAATTAAAAt                                                               >  1:40212/1‑141 (MQ=255)
                                                                        ttttATTACTAGCAGTTTTAACATTTTCACTGCTACGGAACTTTTCGGCCCAGCGTCTCTGCGTAATGTTATCGGCATAAAAATGAGTATGCATATTTTTGATTCCAGGGATTTGACTTTCTTCGGCTTCAATTAAAATTg                                                             <  2:294527/140‑1 (MQ=255)
                                                                        gttgattaATCCCGAGTTTTACAATTTCAATGGCAGGGAATGGTGCGCCCCAGCGTCGATGCGAATTATCATCTGCATAAAAATGTGTAAGCTTTTTTTTGAGTTCAGGTATTTTACTTTCTTCGGCTTCAATTAAAATTg                                                             <  2:294497/137‑1 (MQ=255)
                                                                              taATACCAAGTTTTACAATTTCAATGACAGGGAATGGTGCGCCCCAGCGTCGATGCTAAATATGATCGGCATAAAAATGAGTAAGCATATTTTTGATTTCAGGTATTTTACTTTCTTCGGCTTCAATTAAAATTGAGGTAg                                                       <  2:638828/141‑1 (MQ=255)
                                                                                aTACCAAGTTTTACAATTTCAATGAGAGTGCATGATGAGCCCCAGAATCGATGTTTGATGTGATCGGCATGAAAATGAGTAAGCATGTTTTTGAGTTCAGGGAGTTTGTTTTTTTAGGACTCATTTATAATTGAGATAggg                                                     >  2:197969/1‑141 (MQ=255)
                                                                                aTACCAAGTTTTACAATTTCAATGACAGGGAATGGTGCGCCCCAGCGTCGATGCTAAATATGATCGGCATAAAAATGAGTAAGCATATTTTTGATTTCAGGTATTTTACTTTCTTCGGCTTCAATTTAAATTGAGGTAggg                                                     >  1:78941/1‑141 (MQ=255)
                                                                                aTACCAAGTTTTACAATTTCAATGACAGGGAATGGTGCGCCCCAGCGTCGATGCTAAATATGATCGGCATAAAAATGAGTAAGCATATTTTTGATTTCAGGTATTTTACTTTCTTCGGCTTCAATTAAAATTGAGGTAggg                                                     <  1:537053/141‑1 (MQ=255)
                                                                                aTACCAAGTTTTACAATTTCAATGACAGGGAATGGTGCGCCCCAGCGTCGATGCTAAATATGATCGGCATAAAAATGAGTAAGCATATTTTTGATTTCAGGTATTTTACTTTCTTCGGCTTCAATTAAAATTGAGGTAggg                                                     <  2:269906/141‑1 (MQ=255)
                                                                                  aCCAAGTTTTACAATTTCAATGACAGGGAATGGTGCGCCCCAGCGTCGATGCTAAATATGATCGGCATAAAAATGAGTAAGCATATTTTTGATTTCAGGTATTTTACTTTCTTCGGCTTCAATTAAAATTGAGGTAGGGGa                                                   >  1:243455/1‑141 (MQ=255)
                                                                                  aCCAAGTTTTACAATTTCAATGACAGGGAATGGTGCGCCCCAGCGTCGATGCTAAATATGATCGGCATAAAAATGAGTAAGCATATTTTTGATTTCAGGTATTTTACTTTCTTCGGCTTCAATTAAAATTGAGGTAGGGGa                                                   <  1:506368/141‑1 (MQ=255)
                                                                                  aCCAAGTTTTACAATTTCAATGACAGGGAATGGTGCGCCCCAGCGTCGATGCTAAATATGATCGGCATAAAAATGAGTAAGCATATTTTTGATTTCAGGTATTTTACTTTCTTCGGCTTCAATTAAAATTGAGGTAGGGGa                                                   >  2:184671/1‑141 (MQ=255)
                                                                                    cAAGTTTTACAATTTCAATGACAGGGAATGGTGCGCCCCAGCGTCGATGCTAAATATGATCGGCATAAAAATGAGTAAGCATATTTTTGATTTCAGGTATTTTACTTTCTTCGGCTTCGATTAAAATTGAGGTAGGGGAtt                                                 >  2:108230/1‑141 (MQ=255)
                                                                                      aGTTTTACAATTTCAATGACAGGGAATGGTGCGCCCCAGCGTCGATGCTAAATATGATCGGCATAAAAATGAGTAAGCATATTTTTGATTTCAGGTATTTTACTTTCTTCGGCTTCAATTAAAATTGAGGTAGGGGATTCt                                               >  2:403084/1‑141 (MQ=255)
                                                                                      aGTTTTACAATTTCAAGGACAGGGAATGGTGCGCCCCAGCGTCGATGCTAAATATGATCGGCATAAAAATGAGTAAGCATATTTTTGATTTCAGGTATTTTACTTTCTTCGGCTTCAATTAAAATTGAGGTAGGGGATTCt                                               >  1:348870/1‑141 (MQ=255)
                                                                                       attttACAATTCCAATGAAACGGAATGGTGCGCCCCAGCGTCGATGCTAAATATGATCGGCATAAAAATGAGTAAGCATATTTTTGATTTCAAGTATTTTACTTTCTTCGGCTTCAATTAAAAATGAGGTACGGGATTCtt                                              <  2:290387/140‑1 (MQ=255)
                                                                                       attccaCAATATCAATAACAAGGAATGGTGCGCCCCAGCCACGATGCTAAATATGATCGGCATAAAAATGAGTAAGCATATTTTTGATTTCAGGGATTTTACTTTCTTCGGCTTCAATTAAAATTGAGGTAGGGGATTCtt                                              <  2:175327/136‑1 (MQ=255)
                                                                                        ttttACAATTTCAATGACAGGGAATGGTGCGCCCCAGCGTCGATGCTAAATATGATCGGCATAAAAATGAGTAAGCATATTTTTGATTTCAGGTATTTTACTTTCTTCGGCTTCAATTAAAATTGAGGTAGGGGATTCttt                                             <  1:118326/141‑1 (MQ=255)
                                                                                        ttttACAATTTCAATGACAGGGAATGGTGCGCCCCAGCGTCGATGCTAAATATGATCGGCATAAAAATGAGTAAGCATATTTTTGATTTCAGGTATTTTACTGTCTTCGGCTTCAATTAAAATTGTGGTAGGGGGTTCttt                                             >  1:300320/1‑141 (MQ=255)
                                                                                        ttttACAATTTCAATGACAGGGAATGGTGCGCCCCAGCGTCGATGCTAAATATGATCGGCATAAAAATGAGTAAGCATAGTTTTGATTTCGGGATTTTTACTCTCTTCGGTTTCCAGTAAATTTGCGGTAGGGGGTTCgtt                                             >  2:441886/1‑141 (MQ=255)
                                                                                        ttttACAATTTCAATGACAGGGAATGGTGCGCCCCAGCGTCGATGCTAAATATGATCGGCATAAAAATAAGTAAGCATCTTTTTGATTTCAAGTATTTTACTTTCTTCGGCTTCGATCAGAATTAAGATAGGGGAATCctt                                             >  2:108527/1‑141 (MQ=255)
                                                                                         tttACAATTTCAATGACAGGGAATGGTGCGCCCCAGCGTCGATGCTAAATATGATCGGCATAAAAATGAGTAAGCATATTTTTGATTTCAGGTATTTTACTTTCTTCGGCTTCAATTAAAATTGAGGTAGGGGATTCTTTc                                            >  1:265967/1‑141 (MQ=255)
                                                                                         tttACAATTTCAATGACAGGGAATGGTGCGCCCCAGCATCGATGCTAAATATGATCGGCATAAAAATGAGTAAGCATATTTTTGATTTCAGGTATTTTACTTTCTTCGGCTTCAATTAAAATTGAGGTAGGGGATTCTTTc                                            >  1:235942/1‑141 (MQ=255)
                                                                                           tACAATTTCAATGACAGGGAATGGTGCGCCCCAGCGTCGATGCTAAATATGATCGGCATAAAAATGAGTAAGCATATTTTTGATTTCAGGTATTTTACTTTCTTCGGCTTCAATTAAAATTGAGGTAGGGGATTCTTTCaa                                          >  2:4484/1‑141 (MQ=255)
                                                                                            aCAATTTCAATGACAGGGAATGGTGCGCCCCAGCGTCGATGCTAAATATGATCGGCATAAAAATGAGTAAGCATATTTTTGATTTCAGGTATTTTACTTTCTTCGGCTTCAATTAAAATTGAGGTAGGGGATTCTTTCAAg                                         >  2:483405/1‑141 (MQ=255)
                                                                                            aCAATTTCAATGACAGGGAATGGTGCGCCCCAGCGTCGATGCTAAATATGATCGGCATAAAAATGAGTAAGCATATTTTTGATTTCAGGTATTTTACTTTCTTCGGCTCCAATTAAAATTGGGGTAGGGGATTCTTTCAAg                                         >  1:569766/1‑141 (MQ=255)
                                                                                            aCAATTTCAATGACAGGGAATGGTGCGCCCCAGCGTCGATGCTAAATATGATCGGCATAAAAATGAGTAAGCATATTTTTGATTTCAGGTATGTTACTTTCTTCGGCTTCAATTAAAAGTGAGGTAGCGGCCTCTTTCagg                                         >  2:476409/1‑139 (MQ=255)
                                                                                            aCAATTTCAATGACAGGGAATGGTGCGCCCCAGCGTCGATGCTAAATATAATAGGCATAAAAATGAGTAAGCATATTTTTGATTTCAGGTATTTTACTTTCTTCGGCTTCAATTAAAATTGAGGTAGGGGATTCTTTCAAg                                         >  1:684708/1‑141 (MQ=255)
                                                                                             cAATTTCAATGACAGGGAATGGTGCGCCCCAGCGTCGATGCTAAATATGATCGGCATAAAAATGAGTAAGCATATTTTTGATTTCAGGTATTTTACTTTCTTCGGCTTCAATTAAAATTGAGGTAGGGGATTCTTTCAAGt                                        >  1:627640/1‑141 (MQ=255)
                                                                                               aTTTCAATGACAGGGAATGGTGCGCCCCAGCGTCGATGCTAAATATGATCGGCATAAAAATGAGTAAGCATATTTTTGATTTCAGGTATTTTACTTTCTTCGGCTTCAATTAACATTGAGGTAGGGGATTCTTTCAAGTgg                                      >  2:23410/1‑141 (MQ=255)
                                                                                               aTTTCAATGACAGGGAATGGTGCGCCCCAGCGTCGATGCTAAATATGATCGGCATAAAAATGAGTAAGCATATTTTTGATTTCAGGTATTTTACTTTCTTCGGCTTCAATTAAAATTGAGGTAGGGGATTCTTTCAAGTgg                                      <  1:146745/141‑1 (MQ=255)
                                                                                               aTTTCAATGACAGGGAATGGTGCGCCCCAGCGTCGATGCTAAATATGATCGGCATAAAAATGAGTAAGCATATTTTTGATTTCAGGTATTTTACTTTCTTCGGCTTCAATTAAAATTGAGGTAGGGGATTCTTTCAAGTgg                                      >  2:404066/1‑141 (MQ=255)
                                                                                               aTTTCAATGACAGGGAATGATGCGCCCCCCCGTCGATGCTAGATAAGAACGACAGAAAAAGCAGTAACCATATTTTTGATTTCAGGTATTTTCCTTTCTTCGCCTTCAATTAAAATTGAGGTAGGGGACTATTTCAATTgg                                      >  2:78738/1‑141 (MQ=255)
                                                                                                tttCAATGACAGGGAATGGTGCGCCCCAGCGTCGATGCTAAATATGATCGGCATAAAAATGAGTAAGCATATTTTTGATTTCAGGTATTTTAATTTCTTCGGCTTCAATTAAAATTGAGGTAGGGGATTATTTCAGGTGGa                                     >  1:56839/1‑141 (MQ=255)
                                                                                                tttCAATGACAGGGAATGGTGCGCCCCAGCGTCGATACTAAATATGATCGGCATAAAAATGAGTAAGCATATTTTTGATTTCAGGTATTTTACTTTCTTCGGCTTCAATTAAAATTGAGGTAGGGGATTCTTTCAAGTGGa                                     >  1:616270/1‑141 (MQ=255)
                                                                                                     aTGACAGGGAATGGTGCGCCCCAGCGTCGATGCTAAATATGATCGGCATAAAAATGAGTAAGCATATTTTTGATTTCAGGTATTTTACTTTCTTCGGCTTCAATTAAAATTGAGGTAGGGGATTCTTTCAAGTGGACAAGt                                >  1:262669/1‑141 (MQ=255)
                                                                                                     aTGACAGGGAATGGTGCGCCCCAGCGTCGATGCTAAATATGATCGGCATAAAAATGAGTAAGCATATTTTTGATTTCAGGTATTTTACTTTCTTCGGCTTCAATTAAAATTGAGGTAGGGGATTCTTTCAAGTGGACAAGt                                <  2:304799/141‑1 (MQ=255)
                                                                                                     aTGACAGGGAATGGTGCGCCCCAGCGTCGATGCTAAATATGATCGGCATAAAAATGAGTAAGCATATTTTTGATTTCAGGTATTTTACTTTCTTCGGCTTCAATTAAAATTGAGGTAGGGGATTCTTTCAAGTGAACAAGt                                >  1:642443/1‑141 (MQ=255)
                                                                                                      tGACAGGGAATGGTGCGCCCCAGCGTCGATGCTAAATATGATCGGCATAAAAATGAGTAAGCATATTTTTGATTTCAGGTATTTTACTTTCTTCGGCTTCAATTAAAATTAGGGTAGGGGATTCTTTCAAGTGGACAAGTa                               >  1:122907/1‑141 (MQ=255)
                                                                                                         cAGGGAATGGTGCGCCCCAGCGTCGATGCTAAATATGATCGGCATAAAAATGAGTAAGCATATTTTTGATTTCAGGTATTTTACTTTCTTCGGCTTCAATTAAAATTGAGGTAGGGGATTCTTTCAAGTGGACAAGTAAAt                            <  1:184671/141‑1 (MQ=255)
                                                                                                               aTGGTGCGCCCCAGCGTCGATGCTAAATATGATCGGCATAAAAATGAGTAAGCATATTTTTGATTTCAGGTATTTTACTTTCTTCGGCTTCAATTAAAATTGAGGTAGGGGATTCTTTCAAGTGGACAGGTAAATTACCAg                      >  1:174223/1‑141 (MQ=255)
                                                                                                               aTGGTGCGCCCCAGCGTCGATGCTAAATATGATCGGCATAAAAATGAGTAAGCATATTTTTGATTTCAGGTATTTTACTTTCTTCGGCTTCAATTAAAATTAAGGTAGGGGAATCTTTCAAGTGGACAAGTAAATTACCAg                      >  1:174218/1‑141 (MQ=255)
                                                                                                                tGGTGCGCCCCAGCGTCGATGCTAAATATGATCGGCATAAAAATGAGTAAGCATATTTTTGATTTCAGGTATTTTACTTTCTTCGGCTTCAATTAAAATTGAGGTAGGGGATTCTTTCAAGTGGACAAGTAAATTACCAGt                     >  2:687053/1‑141 (MQ=255)
                                                                                                                 ggTGCGCCCCAGCATCGATGCTAAATATGATCGGCATAAAAATGAGTAAGCATATTTTTGATTTCAGGTATTTTACTTTCTTCGGCTTCAATTAAAATTGAGGTAGGGGATTCTTTCAAGTGGACAAGTAAATTACCAGtt                    <  2:235942/141‑1 (MQ=255)
                                                                                                                    tcgcCCTAGCGTCGATTCTAAATATGATCGGCATAAATATGAGTAAGCATATTTTTGATTTCAGGTATTTTACTTTCTTCGGCTTCAATTAAAATTGAGGTATGGGATTCTTTCAAGTGGACAAGTAAATTACCAGTTTGa                 <  2:27814/140‑1 (MQ=255)
                                                                                                                    gcgcCCCAGCGTCGATGCTAAATATGATCGGCATAAAAATGAGTAAGCATATTTTTGATTTCAGGTATTTTACTTTCTTCGGCTTCAATTAAAATTGAGGTAGGGGATTCTTTCAAGTGGACAAGTAAATTACCAGTTTGa                 >  2:523303/1‑141 (MQ=255)
                                                                                                                          cAGCGTCGATGCTAAATATGATCGGCATAAAAATGAGTAAGCATATTTTTGATTTCAGGTATTTTACTTTCTTCGGCTTCAATTAAAATTGAGGTAGGGGATTCTTTCAAGTGGACAAGTAAATTACCAGTTTGAATTCtt           <  1:340002/141‑1 (MQ=255)
                                                                                                                           accGTCGATGCTAAATATGATCGGTATAAAAATGAGTAAGCATATTTTTGATTTCAGGTATTTTACTTTCTTCGGCTTCAATTAAAATTGAGGTAGGGGATTCTTTCAAGTGGACAAGTAAATTACCAGTTTGAATTCTTg          <  1:69691/139‑1 (MQ=255)
                                                                                                                                cGATGCTAAATATGATCGGCATAAAAATGAGTAAGCATATTTTTGATTTCAGGTATTTTACTTTCTTCGGCTTCAATTAAAATTGAGGTAGGGGATTCTTTCAAGTGGACAAGTAAATTACCAGTTTGAATTCTTGGATTa     >  2:524880/1‑141 (MQ=255)
                                                                                                                                cGATGCTAAATATGATCGGCATAAAAATGAGTAAGCATATTTTTGATTTCAGGTATTTTACTTTCTTCGGCTTCAATTAAAATTGAGGTAGGGGATTCTTTCAAGTGGACAAGTAAATTACCAGTTTGAATTCTTGGATTa     >  1:580270/1‑141 (MQ=255)
                                                                                                                                   cgCTAAATTTGATCGGCATACAAATGAGTAAGCAGATGTTTGATTTCAGGGATATAACATTCAACGGCTTCAATTAAAAGTGAGGTAGGGGATTCTTTCAAGTGGACAAGTAAATTACCAGTTTTTTTTCTTGGaaaaaaa  <  2:306564/140‑7 (MQ=255)
                                                                                                                                       |                                                                                                                                        
CTTCATCACCGAATGCAATAATATTATTTCGGTCAATATTTAAAAATTGTCTAACTTGCTCAATGCCTCTTGCTTTATTAATACCAAGTTTTACAATTTCAATGACAGGGAATGGTGCGCCCCAGCGTCGATGCTCAATATGATCGGCATAAAAATGAGTAAGCATATTTTTGATTTCAGGTATTTTACTTTCTTCGGCTTCAATTAAAATTGAGGTAGGGGATTCTTTCAAGTGGACAAGTAAATTACCAGTTTGAATTCTTGGATTACCC  >  CP000730/973078‑973349

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: