Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 1,063,849 C→T 100% D214N (GAC→AAC)  USA300HOU_1000 ← LytR family transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007301,063,8490CT100.0% 201.7 / NA 62D214N (GAC→AAC) USA300HOU_1000LytR family transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (23/39);  total (23/39)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

AGAGTTAGTTAAATTATGCTTTGCATTTTTACCTACAATTTGAATCACGTTATCAAGTTTATCAATAGAATCAACTTCCTGTGCTTTTTGGAATAAAATCTTAATTAATTCCATTTGACGTTGTCCACGTTTTAAGTCTGAATCATGATGTCTAGTTCTAGCAACTGCTAAAGCCTCATCACCATTTAATTTTTGGTACCCTTTTTTAATTTTAATCTTACCAGTATCATCTGTGTTAGGTTCATTTAAGTCGTATGGCACATCATAGTATATG  >  CP000730/1063712‑1063985
                                                                                                                                         |                                                                                                                                         
agatTTAGGTAAATTATGCTTTGCATTTTTACCTACAATTTGAATCACGTTATCAAGTTTATCAATAGAATCAACTTCCTGTGCTTTTTGGAATAAAATCTTAATTAATTCCATTTGACGTTGTCCACGTTTTAAGTTTGa                                                                                                                                        <  2:131993/141‑1 (MQ=255)
  agttagttAAATTATGCTTTGCATTTTTACCTACAATTTGAATCACGTTATCAAGTTTATCAATAGAATCAACTTCCTGTGCTTTTTGGAATAAAATCTTAATTAATTCCATTTGACGTTGTCCACGTTTTAAGTTTGAAt                                                                                                                                      <  1:15623/141‑1 (MQ=255)
        ttaAATTATGCTTTGCATTTTTACCTACAATTTGAATCACGTTATCAAGTTTATCAATAGAATCAACTTCCTGTGCTTTTTGGAATAAAATCTTAATTAATTCCATTTGACGTTGTCCACGTTTTAAGTTTGAATCatgat                                                                                                                                <  1:653129/141‑1 (MQ=255)
         taAATTATGCTTTGCATTTTTACCTACAATTTGAATCACGTTATCAAGTTTATCAATAGAATCAACTTCCTGTGCTTTTTGGAATAAAATCTTAATTAATTCCATTTGACGTTGTCCACGTTTTAAGTTTGAATCatgatg                                                                                                                               >  1:575152/1‑141 (MQ=255)
           aaTTATGGTTTGCATTTTTACCTACAATTTGAATCACGTTATCAAGTTTATCAATAGAATCAACTTCCTGTGCTTTTTGGAATAAAATCTTAATTAATTCCATTTGACGTTGTCCACGCTTGAGGTTTGAATCATGATGTc                                                                                                                             >  1:433686/1‑141 (MQ=255)
               aTGCTTTGCATTTTTACCTACAATTTGAATCACGTTATCAAGTTTATCAATAGAATCAACTTCCTGTGCTTTTTGGAATAAAATCTTAATTAATTCCATTTGACGTTGTCCACGTTTTAAGTTTGAATCATGATGTCTAGt                                                                                                                         <  1:72294/141‑1 (MQ=255)
                tGCTTTGCATTTTTACCTACAATTTGAATCACGTTATCAAGTTTATCAATAGAATCAACTTCCTGTGCTTTTTGGAATAAAATCTTAATTAATTCCATTTGACGTTGTCCACGTTTTAAGTTTGAATCATGATGTCTAGtt                                                                                                                        >  2:366347/1‑141 (MQ=255)
                tGCTTTGCATTTTTACCTACAATTTGAATCACGTTATCAAGTTTATCAATAGAATCAACTTCCTGTGCTTTTTGGAATAAAATCTTAATTAATTCCATTTGACGTTGTCCACGTTTTAAGTTTGAATCATGATGTCTAGtt                                                                                                                        >  2:12959/1‑141 (MQ=255)
                 gCTTTGCATTTTTACCTACAATTTGAATCACGTTATCAAGTTTATCAATAGAATCAACGTCCTGTGCTTTTTGGAATAAAATCTTAATTAATTCCATTTGACGTTGTCCACGTTTTAAGTTTGAATCATGATGTCTAGTTc                                                                                                                       >  1:419752/1‑141 (MQ=255)
                  cTTTGCATTTTTACCTACAATTTGAATCACGTTATCAAGTTTATCAATAGAATCAACTTCCTGTGCTTTTTGGAATAAAATCTTAATTAATTCCATTTGACGTTGTCCACGTTTTAAGTTTGAATCATGATGTCTAGTTCt                                                                                                                      <  1:7465/141‑1 (MQ=255)
                   tttGCATTTTTACCCACAATTTGAATCACGTTATCAAGTCTATCAATAGAATCAACTTCCTGTGCTTTTTGGACTAAACTCTTAATAACTTCCATTTGTTGTTGTCCTCGTTTTAAGTTTGAATCATGATGTCTAGTTTTa                                                                                                                     <  2:147339/141‑1 (MQ=255)
                   tttGCATTTTTAACTACAATTTGAATCACGTTATCAAGTTTATCAATAGAATCAACTTCCTGTGCTTTTTGGAATAAAATCTTAATTAATTCCATTTGACGTTGTCCACGTTTTAAGTTTGAATCATGATGTCTAGTTCTa                                                                                                                     <  2:234782/141‑1 (MQ=255)
                    ttGCATTTTTACCTACAATTTGAATCACGTTATCAAGTTTATCAATAGAATCAACTTCCTGTGCTTTTTGGAATAAAATCTTAATTAATTCCATTTGACGTTGTCCACGTTTTATGTTTGAATCATGATGTCTAGTTCTAg                                                                                                                    >  1:484202/1‑141 (MQ=255)
                        aTTTTTACCTACAATTTGAATCACGTTATCAAGTTTATCAATAGAATCAACTTCCTGTGCTTTTTGGAATAAAATCTTAATTAATTCCGTTTGACGTTGTCCACGTTTTAAGTTTGAATCATGATGTCTAGTTCTAGCAAc                                                                                                                <  1:270821/141‑1 (MQ=255)
                        aTTTTTACCTACAATTTGAATCACGTTATCAAGGTTATCAATAGAATCAACTTCCTGTGCTTTTTGGAATAAAATCTTAATTAATTCCATTTGACGTTGTCCACGTTTTAAGTTTGAATCATGATGTATAGTTCTAGCaaa                                                                                                                >  2:633827/1‑140 (MQ=255)
                          ttttACCTACAATTTGAATCACGTTATCAAGTTTATCAATAGAATCAACTTCCTGTGCTTTTTGGAATAAAATCTTAATTAATTCCATTTGACGTTGTCCACGTTTTAAGTTTGGATCATGATGTCTAGTTCTAGCAACTg                                                                                                              >  1:72482/1‑141 (MQ=255)
                          ttttACCTACAATTTGAATCACGTTATCAAGTTTATCAATAGAATCAACTTCCTGTGCTTTTTGGAATAAAATCTTAATTAATTCCATTTGACGTTGTCCACGTTTTAAGTTTGAATCATGATGTCTAGTTCTAGCAACTg                                                                                                              >  1:588420/1‑141 (MQ=255)
                           tttACCTACAATTTGAATCACGTTATCAAGTTTATCAATAGAATCAACTTCCTGTGCTTTTTGGAATAAAATCTTAATTAATTCCATTTGACGTTGTCCACGTTTTAAGTTTGAATCATGATGTCTAGTTCTAGCAACTGc                                                                                                             <  2:208833/141‑1 (MQ=255)
                               ccTACAATTTGAATCACGTTATCAAGTTTATCAATAGAATCAACTTCCTGTGCTTTTTGGAATAAAATCTTAATTAATTCCATTTGACGTTGTCCACGTTTTAAGTTTGAATCATGATGTCTAGTTCTAGCAACTGCTaaa                                                                                                         >  2:9179/1‑141 (MQ=255)
                                cTACAATTTGAATTACGTTATCAAGTTTATTGCAAGCGTAAACTTCCTGTACGTTTGTGAAAAAAATCTTGATGAAGCCCAAGTGACGTTGTCCACGGTTTAAGTTTGAATCATGATGTCTAGTTCTAGCAACTGCTAAAg                                                                                                        <  2:60100/141‑1 (MQ=255)
                                 tACAATTTGACTCACGTTATCAAGTTTATCAATAGAATCAACTTCCTGTGCTTGTTGGAATAAAATCTTAATTAATTCCATTTGACGTGGTCCACGTTTTAAGTTTGAATCATGATGTCTAGTTCTAGCAACTGCTAAAGc                                                                                                       <  2:62423/141‑1 (MQ=255)
                                 tACAATTTGAATCACGTTATCATGTTTATCAATAGCATCAACTTCCTGTTCTTGTTGGAATAAAATCTTAATTAATTCCATTTGACGTTGTCCACGTTTTAAGTTTGAATCATGATGTCTAGTTCTAGCAACTGCTAAAGc                                                                                                       <  2:589313/141‑1 (MQ=255)
                                           aTCACGTTATCAAGTTTATCAATAGAATCAACTTCCTGTGCTTTTTGGAATAAAATCTTAATTAATTCCATTTGACGTTGTCCACGTTTTTAGTTGGTATCATGATGTCTAGTTCTCGCAACTGCTAAAGCCTCATCACCa                                                                                             >  1:329327/1‑141 (MQ=255)
                                                        gTTTATCAATAGAATCAACTTCCTGTGCTTTTTGGAATAAAATCTTAATTAATTCCATTTGACGTTGTCCACGTTTTAAGTTTGAATCATGATGTCTAGTTCTAGCAACTGCTAAAGCCTCATCACCATTTAATTTTTGGt                                                                                >  1:232465/1‑141 (MQ=255)
                                                                         aCTTCCTGTGCTTTTTGGAATAAAATCTTAATTAATTCCATTTGACGTTGTCCACGTTTTAAGTTTGAATCATGATGTCTAGTTCTAGCAACTGCTAAAGCCTCATCACCATTTAATTTTTGGTACCCTTttttaatttta                                                              >  1:450717/1‑141 (MQ=255)
                                                                         aCTTCCTGTGCTTTTTGGAATAAAATCTTAATTAATTCCATTTGACGTTGTCCACGTTTTAAGTTTGAATCATGATGTCTAGTTCTAGCAACTGCTAAAGCCTCATCACCATTTAATTTTTGGTACCCTTttttaatttta                                                              <  1:9179/141‑1 (MQ=255)
                                                                            tCCTGTTCTTTTTGGAATAAAATCTTAATTAATTCCATTTGACGGTATCCACGAGTTAACATTGAATCATGATGTCTAGTTCTAGCAACTGCGAAAGCCTCATCACCATTTAATTTTTGGTACCCTTTTTTAATTTTAATc                                                           <  2:271905/141‑1 (MQ=255)
                                                                                ttgCTTTTTGGAATAATATCTTAATTAATTCCATTTGACGTTGTCCACGTTTTAAGTTTGAATCATGATGTCTAGTTCTAGCAACTGCTAAAGCCTCATCACCATTTAATTTTTGGTACCCTTTTTTAATTTTAATCTTAc                                                       <  2:450148/140‑1 (MQ=255)
                                                                                gttcTTTTTGGAATAAAATCTTAATTAATTCCATTTGCCGTTGTCCACGTTTTAAGTTTGAATCATGATGTCTAGTTCTAGCAACTGCTAAAGCCTCATCACCATTTAATTTTTGGTACCCTTTTTTAATTTTAATCTTAc                                                       <  2:113728/138‑1 (MQ=255)
                                                                                gtgCTTTTTGGAATAAAATCTTAATTAATTCCATTTGACGTTGTCCACGTTTTAAGTTTGAATCATGATGTCTAGTTCTAGCAACTGCTAAAGCCTCATCACCATTTAATTTTTAGTCCTCGGTGGTAATTTTAATCTTAc                                                       <  2:669428/141‑1 (MQ=255)
                                                                                     ttttGGAATATATTCTTAATTAATTCCATTTGACGTTGTCCACGTTTTAAGTTTGAATCATGATGTCTAGTTCTAGCAACTGCTAAAGCCTCATCACCATTTAATTTTTGGTACCCTTTTTTAATTTTAATCTTACCAGTa                                                  <  2:44067/141‑1 (MQ=255)
                                                                                     ttttGGAATAAAATCTTAATTAATTCCATTTGACGTTGTCCACGTTTTAAGTTTGAATCATGATGTCTAGTTCTAGCAACTGATGTAGCCTCATCACCATTTAATTTTTGGTACCCTTTTTTAATTTTAATCTTACCAGTa                                                  <  1:649780/141‑1 (MQ=255)
                                                                                      ttttGAATAAAAGCTTAATTAATTCCCTTTGACGTTGTTCACTTTTTAAGGTTGAATCATGATGTCTAGTTCTAGCAACTTCTAAAGCCTTTTCACCATTTTATTTTTGGTACCCCTTTTTTAATTTTAATCTTACCAGTa                                                  <  2:270292/141‑1 (MQ=255)
                                                                                      tttGGAATAAAATCTTCATTAATTCCATTTGACGTTGTCCACGTTTTAAGTTTGAATCATGATGTCTAGTTCTAGCAACTGCTAAAGCCTCATCACCATTTAATTTTTGGTACCCTTttttcgtttttccctgaccattat                                                 <  2:496416/141‑15 (MQ=255)
                                                                                      tttGGAATAAAATCTTAATTAATTCCATTTGACGTTGTCCACGTTTTAAGTTTGAATCATGATGTCTAGTTCTAGCAACTGCTAAAGCCTCATCACCATTTAATTTTTGGTACCCTTTTTTAATTTTAATCTTACCAGTat                                                 <  2:71021/141‑1 (MQ=255)
                                                                                      tttGGAATAAAATCTTAATTAATTCCATTTGACGTTGTCCACGTTTTAAGTTTGAATCATGATGTCTAGTTCTAGCAACTGCTAAAGCCTCATCACCATTTAATTTTTGGTACCCTTTTTTAATTTTAATCTTACCAGTat                                                 <  1:278397/141‑1 (MQ=255)
                                                                                       ttGGAATAAAATCTTAATTAATTCCATTTGACGTTGTCCACGTTTTAAGTTTGAATCATGATGTCTAGTTCTAGCAACTGCTAAAGCCTCATCACCATTTAATTTTTGGTACCCTTTTTTAATTTTAATCTTACCAGTatc                                                <  2:419752/141‑1 (MQ=255)
                                                                                          gAATAAAATCTTAATTAATTCCATTTGACGTTGTCCACGTTTTAAGTTTGAATCATGATGTCTAGTTCTAGCAACTGCTAAAGCCTCATCACCATTTAATTTTTGGTACCCTTTTTTAATTTTAATCTTACCAGTatcttc                                             >  1:520734/1‑141 (MQ=255)
                                                                                            aTAAAATCTTAATTAATTCCATTTGACGTTGTCCACGTTTTAAGTTTGAATCATGATGTCTAGTTCTAGCAACTGCTAAAGCCTCATCACCATTTAATTTTTGGTACCCTTTTTTAATTTTAATCTTACCAGTATCATCtg                                           >  1:29674/1‑141 (MQ=255)
                                                                                             tAAAATCTTAATTAATTCCATTTGACGTTGTCCACGTTTTAAGTTTGAATCATGATGTCTAGTTCTAGCAACTGCTAAAGCCTCATCACCATTTAATTTTTGGTACCCTTTTTTAATTTTAATCTTACCAGTATCATCtgt                                          <  1:230408/141‑1 (MQ=255)
                                                                                             aaaacTCTTAATTAATTCCATTTGACGTTGTCCACGTTTTAAGTTTGAATCATGATGTCTAGTTCTAGCAACTGCTAAAGCCTCATCACCATTTAATTTTTGGTACCCTTTTTTAATTTTAATCTTACCAGTATCATCtgt                                          <  2:616240/140‑1 (MQ=255)
                                                                                              aaaaTCTTAATTAATTCCATTTGACGTTGTCCACGTTTTAAGTTTGAATCATGATGTCTAGTTCTAGCAACTGCTAAAGCCTCATCACCATTTAATTTTTGGTACCCTTTTTTAATTTTAATCTTACCAGTATCATCtgtg                                         <  2:479754/141‑1 (MQ=255)
                                                                                                   cTTAATTAATTCCATTTGACGTTGTCCACGTTTTAAGTTTGAATCATGATGTCTAGTTCTAGCAACTGCTAAAGCCTCATCACCATTTAATTTTTGGTACCCTTTTTTAATTTTAATCTTACCAGTATCATCTGTGTTAgg                                    >  2:611967/1‑141 (MQ=255)
                                                                                                   cTTAATTAATTCCATTTGACGTTGTCCACGTTTTAAGTTTGAATCATGATGTCTAGTTCTAGCAACTGCTAAAGCCTCATCACCATTTAATTTTTGGTACCCTTTTTTAATTTTAATCTTACCAGTATCATCTGTGTTAgg                                    <  2:575152/141‑1 (MQ=255)
                                                                                                        ttaattCCATTTGACGTTGTCCACGTTTTAAGTTTGAATCATGATGTCTAGTTCTAGCAACTGCTAAAGCCTCATCACCATTTAATTTTTGGTACCCTTTTTTAATTTTAATCTTACCAGTATCATCTGTGTTAGGTTCAt                               >  2:293059/1‑141 (MQ=255)
                                                                                                        ttaattCCATTTGACGTTGTCCACGTTTTAAGTTTGAATCATGATGTCTAGTTCTAGCAACTGCTAAAGCCTCATCACCATTTAATTTTTGGTACCCTTTTTTAATTTTAATCTTACCAGTATCATCTGTGTTAGGTTCAt                               <  2:20309/141‑1 (MQ=255)
                                                                                                              ccATTTGACGTTGTCCACGTTTTAAGTTTGAATCATGATGTCTAGTTCTAGCAACTGCTAAAGCCTCATCACCATTTAATTTTTGGTACCCTTTTTTAATTTTAATCTTACCAGTATCATCTGTGTTAGGTTCATTTAAGt                         >  1:49370/1‑141 (MQ=255)
                                                                                                              ccATTTGACGTTGTCCACGTTTTAAGTTTGAATCATGATGTCTAGTTCTAGCAACTGCTAAAGCCTCATCACCATTTAATTTTTGGTACCCTTTTTTAATTTTAATCTTACCAGTATCATCTGTGTTAGGTTCATTTAAGt                         <  1:579232/141‑1 (MQ=255)
                                                                                                                  ttGACTTTGTCCACGTTTTAAGTTTGAATCATGATGTCTAGTTCTGGCAACTGCTAAAGCCTCATCACCATTTAATTTTTGGTACCCTTTTTTAATTTTAATCTTCCCAGTATCATCTGTGTTAGGTTCATTTAAGTCGTa                     <  1:12959/141‑1 (MQ=255)
                                                                                                                  ttGACGTTGTCCACGTTTTAAGTTTGAATCATGATGTCTGGTTCTAGCAACTGCTAAAGCCTCATCCCCATTTAATTTTTGGTACCCTTTTTTATTTTTTTTCTTTCTAGTTTTTTTTGTGTTAGGTTCAttttatttgtt                     >  2:263926/1‑133 (MQ=255)
                                                                                                                  ttGACGTTGTCCACGTTTTAAGTTTGAATCATGATGTCTAGTTCTAGCAACTGCTAAAGCCTCATCACCATTTAATTTTTGGTACCCTTTTTTAATTTTAATCTTACCAGTATCATCTGTGTTAGGTTCATTTAAGTCGTa                     <  1:105152/141‑1 (MQ=255)
                                                                                                                  ttGACGTTGTCCACGTTTTAAGTTTGAATCATGATGTCTAGTTCTAGCAACTGCTAAAGCCTCATCACCATTTAATTTTTGGTACCCTTTTTTAATTTTAATCTTACCAGTATCATCTGTGTTAGGTTCATTTAAGTCGTa                     <  2:361826/141‑1 (MQ=255)
                                                                                                                  ttGACGTTGTCCACGTATAAAGATTGAATCATGATGTCTAGTTCTAGCAACTGCTAAAGCCTCATCACCATTTAATTTTTGGTACCCTTTTTTAATTTTAATCTTACCAGTATCATCTGTGTTAGGTTCATTTAAGTCGTa                     <  1:366347/141‑1 (MQ=255)
                                                                                                                  ttGACCTTGTCCACGTTTTAAGTTTGAATCATGATGTCTAGTTCTAGCAACTGCTAAAGCCTCATCACCATTTAATTTTTGGTACCCTTTTTTAATTTTAATCTTACCAGTATCATCTGTGTTAGGTTCATTTAAGTCGTa                     <  2:556237/141‑1 (MQ=255)
                                                                                                                       gTTGTCCACGTTTTAAGTTTGAATCATGATGTCTAGTTCTAGCAACTGCTAAAGCCTCATCACCATTTAATTTTTGGTACCCTTTTTTAATTTTACTCTTACCAGTATCATCTGTGTTAGGTTCATTTAAGTCGTATGGca                <  1:653463/141‑1 (MQ=255)
                                                                                                                       gTTGTCCACGTTTTAAGTTTGAATCATGATGTCTAGTTCTAGCAACTGCTAAAGCCTCATCACCATTTAATTTTTGGTACCCTTTTTTAATTTTAATCTTACCAGTATCATCTGTGTTAGGTTCATTTAAGTCGTATGGca                <  1:402004/141‑1 (MQ=255)
                                                                                                                         tGTCCACGTTTTAAGTTTGAATCATGATGTCTAGTTCTAGCAACTGCTAAAGCCTCATCACCATTTAATTTTTGTTACCCTTTTTTAATTTTAATCTTACCGATATCATCTGTGTTAGGTTCATTTAAGTCTTATGAcaca              >  2:326526/1‑141 (MQ=255)
                                                                                                                         tGTCCACGTTTTAAGTTTGAATCATGATGTCTAGTTCTAGCAACTGCTAAAGCCTCATCACCATTTAATTTTTGGTACCCTTTTTTAATTTTAATCTTACCAGTATCATCTGTGTTAGGTTCATTTAAGTCGTATGGcaca              <  2:232465/141‑1 (MQ=255)
                                                                                                                           tCCACGTTTTAAGTTTGAATCATGATGTCTAGTTCTAGCAACTGCTAAAGCCTCATCACCATTTAATTTTTGGTACCCTTTTTTAATTTTAATCTTACCAGTATCATCTGTGTTAGGTTCATTTAAGTCGTATGGCAcatc            >  2:364666/1‑141 (MQ=255)
                                                                                                                                gTTTTAAGTTTGAATCATGATGTCTAGTTCTAGCAACTGCTAAAGCCTCATCACCATTTAATTTTTGGTACCCTTTTTTAATTTTAATCTTACCAGTATCATCTGTGTTAGGTTCATTTAAGTCGTATGGCACATCATAGt       <  2:49370/141‑1 (MQ=255)
                                                                                                                                     aaGTTTGAATCATGATGTCTAGTTCTAGCAACTGCTAAAGCCTCATCACCATTTAATTTTTGGTACCCTTTTTTAATTTTAATCTTACCAGTATCATCTGTGTTAGGTTCATTTAAGTCGTATGGCACATCATAGTATATg  >  2:504224/1‑141 (MQ=255)
                                                                                                                                     aaGTTTGAATCATGATGTCTAGTTCTAGCAACTGCTAAAGCCTCATCACCATTTAATTTTTGGTACCCTTTTTTAATTTTAATCTTACCAGTATCATCTGTGTTAGGTTCATTTAAGTCGTATGGCACATCATAGTATATg  >  1:586904/1‑141 (MQ=255)
                                                                                                                                         |                                                                                                                                         
AGAGTTAGTTAAATTATGCTTTGCATTTTTACCTACAATTTGAATCACGTTATCAAGTTTATCAATAGAATCAACTTCCTGTGCTTTTTGGAATAAAATCTTAATTAATTCCATTTGACGTTGTCCACGTTTTAAGTCTGAATCATGATGTCTAGTTCTAGCAACTGCTAAAGCCTCATCACCATTTAATTTTTGGTACCCTTTTTTAATTTTAATCTTACCAGTATCATCTGTGTTAGGTTCATTTAAGTCGTATGGCACATCATAGTATATG  >  CP000730/1063712‑1063985

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: