Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 1,871,583 G→A 5.9% P110L (CCA→CTA)  daaA ← D‑alanine transaminase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007301,871,5830GA 5.9% 194.0 / 3.1 69P110L (CCA→CTA) daaAD‑alanine transaminase
Reads supporting (aligned to +/- strand):  ref base G (38/26);  new base A (2/2);  total (40/29)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.15e-01

CTAATACATTTCCTAATAAGTTCAAGCTTTTAATGTCGCAACGTAACCATCGGATATCTTCAACGGTAACACCATTCACACCATTTTCTAAATGATCATAAGGACGATCATAACTCTTTGTATAAGCAACAATTGCTGGTTCTACTTCAGGTGTCGGGAAGCTATGATTCCTTTCAGCTACACCACGCGTTGCTTGAATATAAATTGCCCCAGTTTCAATTTGATTCATATCAACTAATTTACGAGATAGTTCAATTAATTCTTCTACAGAATAA  >  CP000730/1871446‑1871720
                                                                                                                                         |                                                                                                                                         
cTAATACATTTCCTAATAAGTTCAAGCTTTTAATGTCGCAACGTAACCATCGGATATCTTCAACGGTAACACCATTCACACCATTTTCTAAATGATCATAAGGACGATCATAACTTTTTGTATAACCAACAATTGCTGGtt                                                                                                                                        <  1:350990/141‑1 (MQ=255)
    tACATTTCCTAATAAGTTCAAGCTTTTAATGTCGCAACGTAACCATCGGATATCTTCAACGGTAACACCATTCACACCATTTTCTAAATGATCATAAGGACGATCATAACTCTTTGTATAAGCAACAATTGCTGGTTCTAc                                                                                                                                    >  1:553021/1‑141 (MQ=255)
     aCATTTCCTAATAAGTTCAAGCTTTTAATGTCGCAACGTAACCATCGGATATCTTCAACGGTAACACCATTCACACCATTTTCTAAATGATCCCAAGGACGATGATACATTTCTCAATAATCAACAATTGCTGGTTCTCCt                                                                                                                                   >  2:283133/1‑141 (MQ=255)
     aCATTTCCTAATAAGTTCAAGCTTTTAATGTCGCAACGTAACCATCGGATATCTTCAACGGTAACACCATTCACACCATTTTCTAAATGATCATAAGGACGATCATAACTCTTTGTATAAGCAACAATTGCTGGTTATACt                                                                                                                                   >  2:76552/1‑141 (MQ=255)
        tttCCTAATAAGTTCAAGCTTTTAATATCGCAACGTAACCATCGGATATCTTCAACGGTAACACCATTCACACCATTTTCTACATGCTACTCAGGACGGCAAGACCTCTTTGTATAATCGACAACAGCTGGTTCCACTTcg                                                                                                                                >  2:416592/1‑140 (MQ=255)
         ttCCTAATAAGTTCAAGCTTTTAATGTCGCAACGTAACCATCGGATATCTTCAACGGTAACACCATTCACACCATTTTCTAAATGATCATAAGGACGATCATAACTCTTTGTATAAGCAACAATTGCTAGTTCTACTTCAg                                                                                                                               >  2:630077/1‑141 (MQ=255)
         ctttcaagaggTTCAAGCTTTTAATGTCGCAACGTAACCATCGGATATCTTCAACGGTAACACCATTCACACCATTTTCTAAATGATCATAAGGACGATCATAACTCTTTGTATAAGCAACAATTGCTAGTTCTACTTCAg                                                                                                                               >  2:525188/11‑141 (MQ=255)
          tCCTAATAAGTTCAAGCTTTTAATGTCGCAACGTAACCATCGGATATCTTCAACGGTAACACCATTCACACCATTTTCTAAATGATCATAAGGACGATCATAACTCTTTGTATAAGCAACAATTGCTGGTTCTACTTCAgg                                                                                                                              >  2:363099/1‑141 (MQ=255)
          tCCTAATAAGTTCAAGCTTTTAATGTCGCAACGTAACCATCGGATATCTTCAACGGTAACACCATTCACACCATTTTCTAAATGATCATAAGGACGATCATAACTCTTTGTATAAGCAACAATTGCTGGTTCTACTTCAgg                                                                                                                              >  2:258354/1‑141 (MQ=255)
             taataaGTTCAAGCTTTTAATGTCGCAACGTAACCATCGGATATCTTCAACGGTAACACCATTCACACCATTTTCTAAATGATCATAAGGACGATCATAACTCTTTGTATAAGCAACAATTGCTGGTTCTACTTCAGatgt                                                                                                                           >  1:531708/1‑141 (MQ=255)
               ataaGTTCAAGCTTTTAATGTCGCAACGTAACCATCGGATATCTTCAACGGTAACACCATTCACACCATTTTCTAAATGATCATAAGGACGATCATAACTCTTTGTATAAGCAACAATTGCTGGTTCTACTTCAGGTGTCg                                                                                                                         <  1:688981/141‑1 (MQ=255)
                  aGTTCAAGCTTTTAATGTCGCAACGTAACCATCGGATATCTTCAACGGTAACACCATTCACACCATTTTCTAAATGATCATAAGGACGATCATAACTCTTTGTATAAGCAACAATTGCTGGTTCTACTTCAGGTGTCGGGa                                                                                                                      >  2:681908/1‑141 (MQ=255)
                   gTTCAAGCTTTTAATGTCGCAACGTAACCATCGGATATCTTCAACGGTAACACCATTCACACCATTTTCTAAATGATCATAAGGACGATCATAACTCTTTGTATAAGCAACAATTGCTGGTTCTACTTCAGgtgtagcgcc                                                                                                                     <  2:352031/141‑7 (MQ=255)
                   gTTCAAGCTTTTAATGTCGCAACGTAACCATCGGATATCTTCAACGGTAACACCATTCACACCATTTTCTAAATGATCATAAGGACGATCATAACTCTTTGTATAAGCAACAATTGCTGGTTCTACTTCAGGTGTCGGGaa                                                                                                                     >  1:527969/1‑141 (MQ=255)
                     tCAAGCTTTTAATGTCGCAACGTAACCATCGGATATCTTCAACGGTAACACCATTCACACCATTTTCTAAATGATCATAAGGACGATCATAACTCTTTGTATAAGCAACAATTGCTGGTTCTACTTCAGGTGTCGGGAAGc                                                                                                                   <  1:577818/141‑1 (MQ=255)
                     tCAAGCTTTTAATGTCGCAACGTAACCATCGGATATCTTCAACGGTAACACCATTCACACCATTTTCTAAATGATCATAAGGACGATCATAACTCTTTGTATAAGCAACAATTGCTGGCTCGCCTTCAGCTGTCGGGAGGc                                                                                                                   >  2:540625/1‑141 (MQ=255)
                     tCAAGATTTTAATGTCGTAACGTAACCATCGGATATCTTCAACGGTAACACCATTCACACCATTTTCTAAATGATCATAAGGACGATCATAACTCTTTGTATAAGCAACAATTGCTGGTTCTACTTCAGGTGTCGGGAAGc                                                                                                                   <  1:289774/141‑1 (MQ=255)
                      cAAGCTTTTAATGTCGCAACGTAACCATCGGATATCTTCAACGGTAACACCATTCACACCATTTTCTAAATGATCATAAGGACGATCATAACTCTTTGTATAAGCAACAATTGCTGGTTCTACTTCAGGTGTCGGGAAGCt                                                                                                                  >  1:260609/1‑141 (MQ=255)
                      cAAGCTTTTAATGTCGCAACGTAACCATCGGATATCTTCAACGGTAACACCATTCACACCATTTTCTAAATGATCATAAGGACGATCATAACTCTTTGTATAAGCAACAATTGCTGGTTCTACTTCAGGTGTCGGGAAGCt                                                                                                                  >  1:589866/1‑141 (MQ=255)
                        aGCTTTTAATGTCGCAACGTAACCATCGGATATCTTCAACGGTAACACCATTCACACCATTTTCTAAATGATCATAAGGACGATCATAACTCTTTGTATAAGCAACAATTGCTGGTTCTACTTCAGGTGTCGGGAAGCTAt                                                                                                                <  1:606363/141‑1 (MQ=255)
                           ttttAATGTCGCAACGTAACCATCGGATATCTTCAACGGTAACACCATTCACACCATTTTCTAAATGATCATAAGGACGATCATAACTCTTTGTATAAGCAACAATTGCTGGTTCTACTTCAGGTGTCGGGAAGCTATGAt                                                                                                             <  1:681908/141‑1 (MQ=255)
                            tttAATGTCGCAACGTAACCATCGGATATCTTCAACGGTAACACCATTCACACCATTTTCTAAATGATCATAAGGACGATCATAACTCTTTGTATAAGCAACAATTGCTGGTTCTACTTCAGGTTTCGGGCAGCTATGAtt                                                                                                            >  1:14006/1‑141 (MQ=255)
                            tttAATGTCGCAACGTAACCATCGGATATCTTCAACGGTAACACCATTCACACCATTTTCTAAATGATCATAAAGACGATCATAACTCTTTGTATAAGCAACAATTGCTGGTTCTCCTACAGATTTCGGGAAGCTATGAtc                                                                                                            >  2:418607/1‑140 (MQ=255)
                                aTGTCGCAACGTAACGATCGGATATTTTAAACGGTAACACCATTCACACTATTTTCTAAATGATCATAAGGACGATCATAACTCTTTGTATAAGCAACAATTGCTGGTTCTACTTCAGGTGTCGGGAAGCTATGATTCCtt                                                                                                        <  2:264629/141‑1 (MQ=255)
                                  gTCGCAACGTAACCATCGGATATCTTCAACGGTAACACCATTCACACCATTTTCTAAATGATCATAAGGACGATCATAACTCTTTGTATAAGCAACAATTGCTGGTTCTACTTCAGGTGTCGGGAAGCTATGATTCCTTTc                                                                                                      >  2:536004/1‑141 (MQ=255)
                                  gTCGCAACGTAACCATCGGATATCTTCAACGGTAACACCATTCACACCATTTTCTAAATGATCATAAGGACGATCATAACTCTTTGTATAAGCAACAATTGCTGGTTCTACTTCAGGTGTCGGGAAGCTATGATTCCTTTc                                                                                                      >  2:314045/1‑141 (MQ=255)
                                  gTCGCAACGTAACCATCGGATATCTTCAACGGTAACACCATTCACACCATTTTCTAAATGATCATAAGGACGATCATAACTCTTTGTATAAGCAACAATTGCTGGTTCTACTTCAGGTGTCGGGAAGCTATGATTCCTTTc                                                                                                      >  2:271721/1‑141 (MQ=255)
                                   tCGCAACGTAACCATCGGATATGTTCAACGGTAACACCATTCACACCATTTTCTAAATGATCATAAGGACGATCATAACTCTTTGTATAAGCAACAATTGCTGGTTCTACTTCAGGTGTCGGGGAGCTATGATTCCTTTCa                                                                                                     >  2:194589/1‑141 (MQ=255)
                                    cGCAACGTAACCATCGGATATCTTCAACGGTAACACCATTCACACCATTTTCTAAATGATCATAAGGACGATCATAACTCGTTGTATAAGCAACAATTGCTGGTTCTACTTCAGGTGGCGGGAAGCTAGGATTCCTTTCAg                                                                                                    >  1:285132/1‑141 (MQ=255)
                                        aCGTAACCATCGGATATCTTCAACGGTAACACCATTCACACCATTTTCTAAATGATCATAAGGACGATCATAACTCTTTGTATAAGCAACAATTGCTGGTTCTACTTCCGGTGTCGGGAAGCTATGATTCCTTTCAGCTac                                                                                                >  1:271222/1‑141 (MQ=255)
                                        aCGTAACCATCGGATATCTTCAACGGTAACACCATTCACACCATTTTCTAAATGATCATAAGGACGATCATAACTCTTTGTATAAGCAACAATTGCTGGTTCTACTTCAGGTGTCGGGAAGCTCTGATTCCTTTCAGCTac                                                                                                >  1:22048/1‑141 (MQ=255)
                                            aaCCATCGGATATCTTCAACGGTAACACCATTCACACCATTTTCGAAATGATAATAAGGACGATCATAACTCTTTGTATAAGCAACAATTGTTGGTTCTAATTTAGGGTTCGTTAAGTTATGATTCCTTTCAGTTAcaccc                                                                                            >  1:127854/1‑140 (MQ=255)
                                                ctcagATATCTTCAACGGTAACACCATTCACACCATTTTCTAAATGATCATAAGGACGATCATAACTCTTTGTATAAGCAACAATTGCTGGTTCTACTTCAGGTGTCGGGAAGCTATGATTCCTTTCAGCTACACCAcgcg                                                                                        <  1:215663/137‑1 (MQ=255)
                                                   ggATATCTTCAACGGTAACACCATTCACACCATTTTCTAAATGATCATAAGGACGATCATAACTCTTTGTATAAGCAACAATTGCTGGTTCTACTTCAGGTGTCGGGAAGCTATGATTCCTTTCAGCTACACCACGCGTTg                                                                                     <  1:555514/141‑1 (MQ=255)
                                                      tatCTTCAACGGTAACACCATTCACACCATTTTCTAAATGATCATAAGGACGATCATAACTCTTTGTATAAGCAACAATTGATGGTTCTACTTCAGGTGTCGGGAAGCTATGATTCCTTTCAGCTACACCACGCGTTGCtt                                                                                  <  2:388109/141‑1 (MQ=255)
                                                           tCACCGGTAACACCATTTACCCCCTTTTCTACATGCTGCCAAGAGCCATCCTAACCCTTTGCATAAGCAACAATTGCTGGTGCCACTCCAGGCGTCGTGACCCTCTGATTGCTTTCAGCTACACCACGCGCTGCTTGAata                                                                             <  2:318814/141‑1 (MQ=255)
                                                           tCAACGGTAACACCATTCACACCATTTTCTAAATGATCATAAGGACGATCATAACTCTTTGTATAAGCAACAATTGCTCGTTCCACTTCACTTGTCCGGAAGCTATAATTCCTTTCAGCTACACCACGCGTTGCTTGAata                                                                             <  2:147200/141‑1 (MQ=255)
                                                            cAACGGTAACACCATTCACACCATTTTCTAAATGATCATAAGGACGATCATAACTCTTGGTATAAGCAACAATTGCTGGTTCTACTTCAGGTGTCGGGAAGCTATGATTCCTTTCAGCTACACCACGCGTTGCTTGAatat                                                                            >  1:418360/1‑141 (MQ=255)
                                                              aCGGTAACCCCGTCCAGACCATTTTCGAAAGGATCATAAGGACGATCATAACTCTTTGTATAAGCAACAATTGCTGGTTCTACTTCAGGTGTCGGGAAGCTATGATTCCTTTCAGCTACACCACGCCTTGGTTGAATATaa                                                                          <  2:470934/141‑1 (MQ=255)
                                                                ggTAACACCATTCACACCATTTCCTAAATGATCATAAGGACGATCATAACTCTTTGTATAAGCAACAATTGCTGGTTCTACTTCAGGTGTCGGGAAGCTATGATCCCTTTCAGCTACACCACGCGTTGCTTGAATATAAAt                                                                        <  1:481275/141‑1 (MQ=255)
                                                                gctAACACCATTCACACCATTTTCTAAATGATCATAAGGACGATCATAACTCTTTGTATAAGCAACAATTGCTGGTTCTACTTCAGGTGTCGGGAAGCTATGATTCCTTTCAGCTACACCACGCGTTGCTTGAATATAAAt                                                                        <  1:30668/139‑1 (MQ=255)
                                                                    acacCATTCACACCATTTTCTAAATGATCATAAGGACGATCATAACTCTTTGTATAAGCAACAATTGCTGGTTCTACTTCAGGTGTCGGGAAGCTATGATTCCTTTCAGCTACACCACGCGTTGCTTGAATATAAATTGcc                                                                    <  2:30148/141‑1 (MQ=255)
                                                                       ccATTCACACCATTTTCTAAATGATCATAAGGACGATCATAACTCTTTGTATAAGCAACAATTGCTGGTTCTACTTCAGGTGTCGGGAAGCTATGATTCCTTTCAGCTACACCACGCGTTGCTTGAATATAAATTGCCCCa                                                                 >  2:38310/1‑141 (MQ=255)
                                                                       ccATTCACACCATTTTCTAAATGATCATAAGGACGATCATAACTCTTTGTATAAGCAACAATTGCTGGTTCTACTTCAGGTGTCGGGAAGCTATGATTCCTTTCAGCTACACCACGCGTTGCTTGAATATAAATTGCCCCa                                                                 >  1:552246/1‑141 (MQ=255)
                                                                          ttCACACCATTTTCTAAATGATCATAAGGACGATCATAACTCTTTGTATAAGCAACAATTGCTGGTTCTACTTCAGGTGTCGGGAAGCTATGATTCCTTTCAGCTACACCACGCGTTGCTTGAATATAAATTGCCCCAGtt                                                              >  2:72890/1‑141 (MQ=255)
                                                                            cacaCCATTTTCTAAATGATCATAAGGACGATCATAACTCTTTGTATAAGCAACAATTGCTGGTTCTACTTCAGGTGTCGGGAAGCTATGATTCCTTTCAGCTACACCACGCGTTGCTTGAATATAAATTGCCCCAGTTTc                                                            <  2:260609/141‑1 (MQ=255)
                                                                              cacCATTTTCTAAATGATCATAAGGACGATCATAACTCTTTTTATAAGCAACAATTGCTGGTTCTACTTCAGGTGTCGGGAAGCTATGATTCCTTTCAGCTACACCACGCGTTGCTTGAATATAAATTGCCCCAGTTTCaa                                                          <  2:286614/141‑1 (MQ=255)
                                                                              cacCATTTTCTAAATGATCATAAGGACGATCATAACTCTTTGTATAAGCAACAATTGCTGGTTCTACTTCAGGTGTCGGGAAGCTATGATTCCTTTCAGCTACACCACGCGTTGCTTGAATATAAATTGCCCCAGTTTCaa                                                          <  1:612002/141‑1 (MQ=255)
                                                                                ccATTTTCTAAATGATCATAAGGACGATCATAACTCTTTGTATAAGCAACAATTGCTGGTTCTACTTCAGGTGTCGGGAAGCTATGATTCCTTTCAGCTACACCACGCGTTGCTTGAATATAAATTGCCCCAGTTTCAAtt                                                        >  1:25926/1‑141 (MQ=255)
                                                                                ccATTTTCTAAATGATCATAAGGACGATCATAACTCTTTGTATAAGCAACAATTGCTGGTTATACTTCAGGTGTCGGGAAGCTATGATTCCTTTCAGCTACACCCAGCGTTGCTTGACCATGAATTTACCCaagtccattt                                                        >  2:652586/1‑132 (MQ=255)
                                                                                             gATCATAAGGACGATCATAACTCTTTGTATAAGCAACAATTGCTGGTTCTACTTCAGGTGTCGGGAAGCTATGATTCCTTTCAGCTACACCACGCGTTGCTTGAATATAAATTGCCCCAGTTTCAATTTGATTCATATCaa                                           <  2:594928/141‑1 (MQ=255)
                                                                                                   aaGGACGATCATAACTCTTTGTATAAGCAACAATTGCTAGTTCTACTTCAGGTGTCGGGAAGCTATGATTCCTTTCAGCTACACCACGCGTTGCTTGAATATAAATTGCCCCAGTTTCAATTTGATTCATATCAACTAAtt                                     <  1:630077/141‑1 (MQ=255)
                                                                                                   aaGGACGATCATAACTCTTTGTATAAGCAACAATTGCTAGTTCTACTTCAGGTGTCGGGAAGCTATGATTCCTTTCAGCTACACCACGCGTTGCTTGAATATAAATTGCCCCAGTTTCAATTTGATTCATATCAACTAAtt                                     <  1:525188/141‑1 (MQ=255)
                                                                                                       aCGATCATAACTCTTTGTATAAGCAACAATTGCTGGTTCTACTTCAGGTGTCGGGAAGCTATGATTCCTTTCAGCTACACCACGCGTTGCTTGAATATCAATTGCCCCAGTTTCAATTTGATTCATATCAACTAATTTACg                                 >  2:27953/1‑141 (MQ=255)
                                                                                                       aCGATCATAACTCTTTGTATAAGCAACAATTGCTGGTTCTACTTCAGGTGTCGGGAAGCTATGATTCCTTTCAGCTACACCACGCGTTGCTTGAATATAAATTGCCCCAGTTTCAATTTGATTCATATCAACTAATTTACg                                 >  1:390426/1‑141 (MQ=255)
                                                                                                       aCGATCATAACTCTTTGTATAAGCAACAATTGCTGGTTCTACTTCAGGTGTCGGGAAGCTATGATTCCTTTCAGCTACACCACGCGTTGCTTGAATATAAATTGCCCCAGTTTCAATTTGATTCATATCAACTAATTTACg                                 >  1:388197/1‑141 (MQ=255)
                                                                                                           tCATAACTCTTTTTATAAGCAACAATTGCTGGTTCTACTTCAGGTGTCGGGAAGCTATGATTCCTTTCAGCTACACCACGCGTTGCTTGAATATAAATTGCCCCATTTTCAATTTGATTCATATCAACTAATTTACGAGAt                             <  2:122281/141‑1 (MQ=255)
                                                                                                           tCATAACTCTTTGTATAAGCAACAATTGCTGGTTCTACTTCAGGTGTCGGGAAGCTATGATTCCTTTCAGCTACACCACGCGTTGCTTGAATATAAATTGCCCCAGTTTCAATTTGATTCATATCAACTAATTTACGAGAt                             >  1:672865/1‑141 (MQ=255)
                                                                                                           tCATAACTCTTTGTATAAGCAACAATTGCTGGTTCTACTTCAGGTGTCGGGAAGCTATGATTCCTTTCAGCTACACCACGCGTTGCTTGAATATAAATTGCCCCAGTTTCAATTTGATTCATATCAACTAATTTACGAGAt                             >  1:282766/1‑141 (MQ=255)
                                                                                                             aTAACTCTTTGTATAAGCAACTATTGCTGGTTCTACTTCAGGTGTCGGGAAGCTATGATTCCTTTCAGCTACACCACGCGTTGCTTGAATATAAATTGCCCCAGTTTCAATTTGATTCATATCAACTAATTTACGAGATAg                           >  1:361228/1‑141 (MQ=255)
                                                                                                             aTAACTCTTTGTATAAGCAACAATTGCTGGTTCTACTTCAGGTGTCGGGAAGCTATGATTCCTTTCAGCTACACCACGCGTTGCTTTAATATAAATTGCCCCAGTTTCAATTTGATTCATATCAACTAATTTACGAGATAg                           <  1:450456/141‑1 (MQ=255)
                                                                                                               aaCTCTTTGTATAAGCAACAATTGCTGGTTCTACTTCAGGTGTCGGGAAGCTATGATTCCTTTCAGCTACACCACGCGTTGCTTGAATATAAATTGCCCCAGTTTCAATTTGATTCATATCAACTAATTTACAATATAGtt                         >  1:170409/1‑141 (MQ=255)
                                                                                                                          taAGCAACAATTGCTGGTTCTACTTCAGGTGTCGGGAAGCTGTGATTCCTTTCAGCTACACCACGCGTTGCTTGAATATAAATTGCCCCAGCTTCCATTTGCTTCATATCAACTAATTTACGAGATAGTTCAATTAAttct              >  2:195756/1‑141 (MQ=255)
                                                                                                                             gCAACAATTGCTGGTTCTACTTCAGGTGTCGGGAAGCTATGATTCCTTTCAGCTACACCACGCGTTGCTTGAATATAAATTGCCCCAGTTTCAATTTGATTCATATCAACTAATTTACGAGATAGTTCAATTAATtcttct           <  1:295104/141‑1 (MQ=255)
                                                                                                                                acaaTTGCTGGTTCTACTTCAGGTGTGTGGAAGCTATGATTCCTTTCAGCTACACCACGCGTTGCTTGAATATAAATTGCCCCAGTTTCAATTTGATTCATATCAACTAATTTACGAGATAGTTCATTTAATTCTTCTAcc        >  1:510502/1‑140 (MQ=255)
                                                                                                                                 caaTTGCTGTGTCCACTTCAGGTGTCGGGAAGCTATGATTCCTTTCAGCTACACCACGCGTTGCTTGAATATAAATTGCCCCAGTTTCAATTTGACTCATATCAACTAATTTACGAGATAGTTCAATTAATTCTTCTACAg       <  2:271222/141‑1 (MQ=255)
                                                                                                                                 caaTTGCTGGTTCTACTTCAGGTGTCGGGAAGCTATGATTCCTTTCAGCTACACCACGCGTTGCTTGAATATAAATTGCCCCAGTTTCAATTTGATTCATATCAACTAATTTACGAGATAGTTCAATTAATTCTTCTACAg       >  2:275225/1‑141 (MQ=255)
                                                                                                                                 caaTTGCTGGGTCTACTTCAGGTGTCGGGAAGCTATGATTCCTTTCAGCTACACCACGCGTTGCTTGAATATAAATTGCCCCAGTTTCAATTTGATTCATATCAACTAATTTACGAGATAGTTCAATTAATTCTTCTACAg       <  2:22048/141‑1 (MQ=255)
                                                                                                                                    tggcgggTCCTACGTCAGTCGTCGCGAAGCTATGATGCCTTGCAGTTCCACCACGCATTGCTTGAATATAAATCGCCCCAGTATCAATTTGATTCATATCAACTAATTTAAGAGTTAGTTCAATTAATTCTTCTACAGaat    <  2:216734/136‑1 (MQ=255)
                                                                                                                                      tgtGGTTCTACTTCAGTTGTCGGGAAGCTATGCTTCCTTGCAGCTACACCACGCGTTGCTTGAATATAAATTGCCCCAGTTTTAATTTGATTCATATCAACTAATTTACGATATAGTTCAATTGATTCTTCGACAGaataa  <  2:671277/139‑1 (MQ=255)
                                                                                                                                         |                                                                                                                                         
CTAATACATTTCCTAATAAGTTCAAGCTTTTAATGTCGCAACGTAACCATCGGATATCTTCAACGGTAACACCATTCACACCATTTTCTAAATGATCATAAGGACGATCATAACTCTTTGTATAAGCAACAATTGCTGGTTCTACTTCAGGTGTCGGGAAGCTATGATTCCTTTCAGCTACACCACGCGTTGCTTGAATATAAATTGCCCCAGTTTCAATTTGATTCATATCAACTAATTTACGAGATAGTTCAATTAATTCTTCTACAGAATAA  >  CP000730/1871446‑1871720

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑