Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 2,502,114 A→C 4.7% H213P (CAT→CCT)  USA300HOU_2364 → AraC transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007302,502,1140AC 4.7% 240.6 / 4.5 85H213P (CAT→CCT) USA300HOU_2364AraC transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base A (40/41);  new base C (2/2);  total (42/43)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.56e-01

AAAGATATAGCGATGCATTGCAACATCTCTGAATCATATTGTTCTAATCTTTTTGTTCGATATTTGAGTATGAATTTTAAAGATTATTTTACAAGTATTAAACTTGTTAACGCCATCAATTTATTACTTTCTACAAAACATTCTATTACAACTGTTTCTGAATTGGCCGGTTTTAACAGTCATACAAATTTTGCAAATCAATTTAAGAATTACTTGCATTTTAGTCCTAAACAGTTCCGTTCACTCGTTTCCAAGATTACTGAACCACCACAAATACAC  >  CP000730/2501975‑2502253
                                                                                                                                            |                                                                                                                                           
aaaGATATAGCGATGCATTGCAACATCTCTGAATCATATTGTTCTAATCTTTTTGTTCGATATTTGAGTATGAATTTTAAAGATTATTTTACAAGTATTAAACTTGTTAACGCCATCAATTTATTACTTTCTACAAAACAt                                                                                                                                            <  2:494877/141‑1 (MQ=255)
aaaGATATAGCGATGCATTGCAACATCTCTGAATCATATTGTTCTAATCTTTTTGTTCGATATTTGAGTATGAATTTTAAAGATTATTTTACAAGTATTAAACTTGTTAACGCCATCAATTTATTACTTTCTACAAAACAt                                                                                                                                            <  1:74169/141‑1 (MQ=255)
   gATATAGCGATGCATTGCAACATCTCTGAATCATATTGTTCTAATCTTTTTGTTCGATATTTGAGTATGAATTTTAAAGATTATTTTACAAGTATTAAACTTGTTAACGCCATCAATTTATTACTTTCTACAAAACATTCt                                                                                                                                         <  2:263885/141‑1 (MQ=255)
      atggCGTTGCATTGCAACATCTCTGAATCATATTGTTCTAATCTTTTTGTTCGATATTTGAGTATGAATTTTAAAGATTATTTTACAAGTATTAAACTTGTTAACGCCATCAATTTATTACTTTCTACAAAACCTTCTAtt                                                                                                                                      <  2:767/138‑1 (MQ=255)
        ggCGATGCATAGCAACATCTCTTATTCATATTGTTCTAATCTTTTTGTTCGATATTTGAGTATGAATTTTAAAGATTATTTTACAAGTATTAAACTTGTTAACGCCATCAATTTATTACTTTCTACAAAACATTCTATTAc                                                                                                                                    <  2:256323/140‑1 (MQ=255)
        atcGATGCATTGCAACATCTCTGAATCATATTGTTCTAATCTTTTTGTTCGATATTTGAGTATGAATTTTAAAGATTATTTTACAAGTATTAAACTTGTTAACGCCATCAATTTATTACTTTCTACAAAACATTCTATTAc                                                                                                                                    <  2:20476/139‑1 (MQ=255)
         gCGATGCATTGCAACATCTCTGAATCATATTGTTCTAATCTTTTTGTTCGATATTTGAGTATGAATTTTAAAGATTATTTTACAAGTATTAAACTTGTTAACGCCATCAATTTATTACTTTCTACAAAACATTCTATTACa                                                                                                                                   >  2:275590/1‑141 (MQ=255)
         gCGATGCATTGCAACATCTATGAATCATATTGTTCTAATCTTTTTGTAAGATATTTGAGTATGAATTTTAAAGATTATTTTACAAGTATTAAACTTGTTAACGCCATCAATTTATTACTTTCTACAAAACATTCTATTACa                                                                                                                                   >  2:478275/1‑141 (MQ=255)
            aTGCATTGCAACATCTCTGAATCATATTGTTCTAATCTTTTTGTTCGATATTTGAGTATGAATTTTAAAGATTATTTTACAAGTATTAAACTTGTTAACGCCATCAATTTATTACTTTCTACAAAACATTCTATTACAACt                                                                                                                                >  1:591385/1‑141 (MQ=255)
              tcATTGCAACATCTCTGAATCATATTGTTCTAATCTTTTTGTTCGATATTTGAGTATGAATTTTAAAGATTATTTTACAAGTATTAAACTTGTTAACGCCATCAATTTATTACTTTCTACAAAACATTCTATTACAACTGt                                                                                                                              <  2:270909/140‑1 (MQ=255)
                 ttGCAACATCTCTGAATCATATTGTTCTGATCTTTTTGTTCGATATTTGAGTATCAATTTTAATGATTATTTTACAGGTATTAAACTTGTTAACTCCTTCATTTTATTACTTTCTACAATACATTTTATTACAAATGTTTc                                                                                                                           >  1:450106/1‑141 (MQ=255)
                 ttGCAACATCTCTGAATCATATTGTTCTAATCTTTTTGTTCGATATTTGAGTATGAATTTTAAAGATTATTTTACAAGTATTAAACTTGTTAACGCCATCAATTTATTACTTTCTACAAAACATTCTATTACAACTgaatt                                                                                                                           >  1:674431/1‑137 (MQ=255)
                    cAACATCTCTGAATCATATTGTTCTAATCTTTTTGTTCGATATTTGAGTATGAATTTTAAAGATTATTTTACAAGTATTAAACTTGTTAACGCCATCAATTTATTACTTTCTACAAAACCTTCTATTACa                                                                                                                                   >  2:242602/1‑130 (MQ=255)
                    cAACATCTCTGAATCATATTGTTCTAATCTTTTTGTTCGATATTTGAGTATGAATTTTAAAGATTATTTTACAAGTATTAAACTTGTTAACGCCATCAATTTATTACTTTCTACAAAACCTTCTATTACa                                                                                                                                   <  1:242602/130‑1 (MQ=255)
                        aTCTCTGAATCATATTGTTCTAATCTTTTTGTTCGATATTTGAGTATGAATTTTAAAGATTATTTTACAAGTATTAACTGTGGCTATTCCGTCAATTTATTACTTTCTACAAAACATTCTATTACAACTGTTTCTGAATTg                                                                                                                    >  1:331918/1‑141 (MQ=255)
                        aTCTCTGAATCATATTGTTCTAATCTTTTTGTTCGATATTTGAGTATGAATTTTAAAGATTATTTTACAAGTATTAAACTTGTTAAAGGCAGGTATTTAAGACTTTCTACAAAAAATTCTATTACAACTGTTTCTGAATTg                                                                                                                    >  1:677634/1‑141 (MQ=255)
                        aTCTCTGAATCATATTGTTCTAATCTTTTTGTCCGATCTTTGAGTCTCAATGTAGGGGCCAATTTTGTACGTATTAAACTTGTTATCGCCATCTATTTATTACTTTCTACAAAACATTCTATTACAACTGTTTCTGAATTg                                                                                                                    <  2:664673/141‑1 (MQ=255)
                          ttctGAACCATATTGTTCTAATCTTTTTTTTCGATATTTGAGTATGAATTTTAAAGATTATTTTACAAGTATTAAACTTGTTAACGCCATCAATTTATTACTTTCTACAAAACATTCTATTACAACTGTTTCTGAATTGGc                                                                                                                  <  2:155947/140‑1 (MQ=255)
                            ctGAATCATATTGTTCTAATCTTTTTGTTCGATGTTTGAGTTTGAATTTTAAAGATTATTTTACACGTATTAAACTTGTTAACGCCATCAATTTATTACTTTCTACAAAACATTCTATTACAACTGTTTCTGAATTGGCCg                                                                                                                <  1:298640/141‑1 (MQ=255)
                            ctGAATCATATTGTTCTAATCTTTTTGTTCGATATTTGAGTATGAATTTTAAAGATTATTTTACAAGTATTAAACTTGTTAACGCCATCAATTTATTACTTTCTACAAAACATTCTATTACAACTGTTTCTGAATTGGCCg                                                                                                                <  2:678975/141‑1 (MQ=255)
                               aaTCATATTGTTCTAATCTTTTTGTTTTTTTTTTGAGTATGAATTTTAAAGATTATTTTACAAGTATTAAACTTGTTAACGCCATCAATTTATTACTTTCTACAAAACATTCTATTACAACTGTTTCTGAATTGTccttgt                                                                                                             >  2:353376/1‑137 (MQ=255)
                               aaTCATATTGTTCTAATCTTATTATTGTTGTGGTGAGTATGAATTTTAAAGATTATTTTACTAGTATTAAACTTGTTAACGCCATCAATTTATTACTTTCTACAAAACATTCTATTAAAACTGTTTCTGAAttttccgttt                                                                                                             >  2:149413/1‑133 (MQ=255)
                                     atTGTTCTAATCTTTTTGTTCGATATTTGAGTATGAATTTTAAAGATTATTTTACAAGTATTAAACTTGTTAACGCCATCAATTTATTACTTTCTACAAAACATTCTATTACAACTGTTTCTGAATTGGCCGGTTTTAACa                                                                                                       >  1:664897/1‑141 (MQ=255)
                                           ttAATCTTTTTGTTCGATATTTGAGTATGAATTTTAAAGATTATTTTACAAGTATTAAACTTGTTAACGCCATCAATTTATTACTTTCTACAAAACATTCTATTACAACTGTTTCTGAATTGGCCGGTTTTAACAGTCATa                                                                                                 <  2:227493/140‑1 (MQ=255)
                                           ttAATCTTTTTGTTCGATATTGGAGTATGAATTTTAAAGAGTATTTTACAAGTATTAAACTTGTTAACGCCATCAATTTATTACTTTCTACAAAACATTCTATTACAACTGTTTCTGAATTGGCCGGTTTTAACAGTCATa                                                                                                 <  1:170475/140‑1 (MQ=255)
                                           cTAATCTTTTTGTTCGATATTTGAGTATGAATTTTAAAGATTATTTTACAAGTATTAAACTTGTTAACGCCATCAATTTATTACTTTCTACAAAACATTCTATTACAACTGTTTCTGAATTGGCCGGTTTTAACAGTCATa                                                                                                 <  2:571727/141‑1 (MQ=255)
                                             aaTCTTTTTGTTCGATATTTGAGTATGAATTTTAAAGATTATTTTACAAGTATTAAACTTGTTAACGCCATCAATTTATTACTTTCTACAAAACATTCTATTACAACTGTTTCCGAATTGGCCGGTTTTAACAGTCATACa                                                                                               >  1:395976/1‑141 (MQ=255)
                                              aTCTTTTTGTTCGATATTTGAGTATGAATTTTAAAGATTATTTTACAAGTATTAAACTTGTTAACGCCATCAATTTATTACTTTCTACAAAACATTCTATTACAACTGTTTCTTAATTGGCCGGTTTTAACAGTCAtcaaa                                                                                              >  2:187451/1‑137 (MQ=255)
                                                cTTTTTGTTCGATATTTGAGTATGAATTTTAAAGATTATTTTACAAGTATTAAACTTGTTAACGCCATCAATTTATTACTTTCTACAAAACATTCTATTAGAACTGTTTCTGAATTGGCCGGTTTTAACAGTCATACAAAt                                                                                            >  2:354873/1‑141 (MQ=255)
                                                cTTTTTGTTCGATATTTGAGTATGAATTTTAAAGATTATTTTACAAGTATTAAACTTGTTAACGCCATCAATTTATTACTTTCTACAAAACATTCTATTACAACTGTTTCTGTATTGGCCGGTTTTAACAGTCATACAAAt                                                                                            >  2:416052/1‑141 (MQ=255)
                                                cTTTTTGTTCGATATTTGAGTATGAATTTTAAAGATTATTTTACAAGTATTAAACTTGTTAACGCCATCAATTTATTACTTTCTACAAAACATTCTATTACAACTGTGTCTGAATTGGCCGGTTTTAACAGTCATACAAAt                                                                                            <  1:147096/141‑1 (MQ=255)
                                                cTTTTTGTTCGATATCTGAGTATGAATTTTAAAGATTATTTTACAAGTATTAAACTTGTTAACGCCATCAATTTATTACTTTCTGCAAAACAATCTATTACAACTGTTTCTGAATTGGCCGGTTTTAACAGTCATACAAAt                                                                                            >  1:512278/1‑141 (MQ=255)
                                                    ttGTTCGATATTTGAGTATGAATTTTAAAGATTATTTTACAAGTATTAAACTTGTTAACGCCATCAATTTATTACTTTCTACAAAACATTCTATTACAACTGTTTCTGAATTGGCCGGTTTTAACAGTCATACAAATTTTg                                                                                        >  1:276130/1‑141 (MQ=255)
                                                      gTTCGATATTTGAGTATGAATTTTAAAGATTATTTTACAAGTATTAAACTTGTTAACGCCATCAATTTATTACTTTCTACAAAACATTCTATTACAACTGTTTCTGAATTGGCCGGTTTTAACAGTCATACAAATTTTGCa                                                                                      >  1:100388/1‑141 (MQ=255)
                                                      gTTCGATATTTGAGTATGAATTTTAAAGATTATTTTACAAGTATTAAACTTGTTAACGCCATCAATTTATTACTTTCTACAAAACATTCTATTACAACTGTTTCTGAATTGGCCGGTTTTAACAGTCATACAAATTTTGCa                                                                                      >  1:639898/1‑141 (MQ=255)
                                                       ttCTATATTTGAGTATGAATTTTAAAGATTATTTTACAAGTATTAAACTTGTTAACGCCATCAATTTATTACTTTCTACAAAACATTCTATTACAACTGTTTCTGAATTGGCCGGTTTTAAAAGTCATACAAATTTTGCaa                                                                                     >  2:409624/1‑141 (MQ=255)
                                                       ttCGATATTTGAGTATGAATTTTAAAGATTATTTTACAAGTATTAAACTTGTTAACGCCATCAATTTATTACTTTCTACAAAACATTCTATTACAACTGTTTCTGAATTGGCCGGTTTTAACAGTCATACAAATTTTGCaa                                                                                     >  2:653750/1‑141 (MQ=255)
                                                            tatTTGAGTATGAATTTTAAAGATTATTTTACAAGTATTAAACTTGTTAACGCCATCAATTTATTACTTTCTACAAAACATTCTATTACAACTGTTTCTGAATTGGCCGGTTTTAACAGTCATACAAATTTTGCAAATCaa                                                                                <  1:526233/141‑1 (MQ=255)
                                                            tatTTGAGTATGAATTTTAAAGATTATTTTACAAGTATTAAACTTGTTAACGCCATCAATTTATTACTTTCTACAAAACATTCTATTACAACTGTTTCTGAATTGGCCGGTTTTAACAGTCATACAAATTTTGCAAATCaa                                                                                <  1:526610/141‑1 (MQ=255)
                                                                 tGAGTATGAATTTTAAAGATTATTTTACAAGTATTAAACTTGTTAACGCCATCAATTTATTACTTTCTACAAAACATTCTATTACAACTGTTTCTGAATTGGCCGGTTTTAACAGTCATCCAAATTTTGCAAATCAATTTa                                                                            >  2:540579/1‑141 (MQ=255)
                                                                   aGTATGAATTTTAAAGATTATTTTACAAGTATTAAACTTGTTAACGCCATCAATTTATTACTTTCTACAAAACATTCTATTACAACTGTTTCTGAATTGGCCGGTTTTAACAGTCATACAAATTTTGCAAATCAATTTAAg                                                                          >  1:637489/1‑141 (MQ=255)
                                                                      aTGAATTTTAAAGATTAGTTTACAAGTATTAAACTTGTTAACGCCATCAATTTATTACTTTCTACAAAACATTCTATTACAACTGTTTCTGAAGTGGCCGGTTTTAACAGTCATACAAATTTTGCAAATCAATTTAAGAAt                                                                       <  2:449686/141‑1 (MQ=255)
                                                                        gAATTTTAAAGATTATTTTACAAGTATTAAACTTGTTAACGCCATCAATTTATTAATTTCTGCAAGACATTCTATTACAACTGTTTCTGAATTGGCCGGTTTTACAAGGCATACCAATTTTGCAGATAAGTTTatcgattc                                                                     >  1:576814/1‑134 (MQ=255)
                                                                           ttttAAAGATTATTTTACAAGTATTAAACTTGTTAACGCCATCAATTTATTACTTTCTACAAAACATTCTATTACAACTGTTTCTGAATTGGCCGGTTTTAACAGTCATACAAATTTTGCAAATCAATTTACGAATTACtt                                                                  >  1:557192/1‑141 (MQ=255)
                                                                             ttAAAGATTATTTTACAAGTATTAAACTTGTTAACGCCATCAATTTATTACTTTCTACAAAACATTCTATTACAACTGTTTCTGAATTGGCCGGTTTTAACAGTCATACAAATTTTGCAAATCAATTTAAGAATTACTTGc                                                                <  1:556230/141‑1 (MQ=255)
                                                                              tAAAGAGTATTTTACAAGTATTAAACTTGTTAACGCCATCAATTTATTACTTTCTACAAAACATTCTATTACAACTGTTTCTGAATTGGCCGGTTTTAACAGTCATACAAATTTTGCAAATCAATTTAAGAATTACTTGCa                                                               <  2:141755/141‑1 (MQ=255)
                                                                                       ttttACAAGTATTAAACTTGTTAACGCCATCAATTTATTACTTTCTACAAAACATTCTATTACAACTGTTTCTGAATTGGCCGGTTTTAACAGTCATACAAATTTTGCAAATCAATTTAAGAATTACTTGCATTTTAGTcc                                                      >  2:240125/1‑141 (MQ=255)
                                                                                         ttACAAGTATTAAACTTGTTAACCCCATCAATTTATTACTTTCTACAAAACATTCTATTACAACTGTTTCTGAATTGGCCGGTTTTAACAGTCATACAAATTTTGCAATTCAATTTAAGAATTACTTGCATTTTAGTCCTa                                                    >  2:631960/1‑141 (MQ=255)
                                                                                           aCAAGTATTAAACTTGTTAACGCCATCAATTTATTACTTTCTACAAAACATTCTATTACAACTGTTTCTGAATTGGCCGGTTTTAACAGTCATACAAATTTTGCAAATCAATTTAAGAATTACTTGCATTTTAGTCCTaaa                                                  <  1:593961/141‑1 (MQ=255)
                                                                                           aCAAGTATTAAACTTGTTAACGCCATCAATTTATTACTTTCTACAAAACATTCTATTACAACTGTTTCTGAATTGGCCGGTTTTAACAGTCATACAAATTTTGCAAATCAATTTAAGAATTACTTGCATTTTAGTCCTaaa                                                  <  1:611980/141‑1 (MQ=255)
                                                                                              aGTATTAAACTTGTTAACGCCATCAATTTATTACTTTCTACAAAACATTCTATTACAACTGTTTCTGAATTGGCCGGTTTTAACAGTCATACAAATTTTGCAAATCAATTTAAGAATTACTTGCATTTTAGTCCTAAACAg                                               <  2:638570/141‑1 (MQ=255)
                                                                                                tATTAAACTTGTTAACGCCATCAATTTATTACTTTCTACAAAACATTCTATTACAACTGTTTCTGAATTGGCCGGTTTTAACAGTCATACAAATTTTGCAAATCAATTTAAGAATTACTTGCATTTTAGTCCTAAACAGtt                                             >  1:213468/1‑141 (MQ=255)
                                                                                                tATTAAACTTGTTAACGCCATCAATTTATTACTTTCTACAAAACATTCTATTACAACTGTTTCTGAATTGGCCGGTTTTAACAGTCATACAAATTTTGCAAATCAATTTAAGAATTACTTGCATTTTAGTCCTAAACAGtt                                             >  1:213517/1‑141 (MQ=255)
                                                                                                     aaCTTGTTAACGCCATCAATTTATTACTTTCTACAAAACATTCTATTACAACTGTTTCTGAATTGGCCGGTTTTAACAGTCATACAAATTTTGCAAATCAATTTAATAATTACTTGCATTTTAGTCCTAAACAGTTCCGtt                                        >  2:160595/1‑141 (MQ=255)
                                                                                                      aCTTGTTAACGCCATCAATTTATTACTTTCTACAAAACATTCTATTACAACTGTTTCTGAATTGGCCGGTTTTAACAGTCATACAAATTTTTCAAATCAATTTAAGAATTACTTGCATTTTAGTCCTAAACAGTTCCGTTc                                       <  2:677982/141‑1 (MQ=255)
                                                                                                        ttGGTAACCCCATCAATTTATTACTTTCTACAAAACATTCTTTTACAACTTTTTCCGAATTGGCCGGTTTTAACAGTCATAAAAATTTTGCAAATCAATTTAAGAATTACTTTCATTTTAGTCCTAAACAGTTCCGTTccc                                     <  2:395976/141‑3 (MQ=255)
                                                                                                         tGTTAACGCCGTCAATTTAGTACTTTCTACAAAACATTCTATTACAACTTTTTCTGCAGTGGCCGGTTTGAACAGTCATACAAGTCTGGCAAAGCAATTTAAGAAGTACTTGCATTTTAGTCCTAAACAGTTCCGTTCACt                                    <  2:92880/141‑1 (MQ=255)
                                                                                                         tGTTAACGCCATCAATTTATTACTTTCTACAAAACATTCTATTACAACTGTTTCTGAATTGGCCGGTTTTAACAGTCATACAAATTTTGCAAATCAATTTAAGAATTACTTGCATTTTAGTCCTAAACAGTTCCGTTCACt                                    >  1:621388/1‑141 (MQ=255)
                                                                                                          gTTAACGCCATCAATTTATTACTTTCTACAAAACCTTCTATTACAACTGTTTCTGAATTGGCCGGTTTTAACAGTCATACAAATTTTGCAAATCAATTTAAGAATTACTTGCATTTTAGTCCTAAACAGTTCCGTTCACTc                                   >  1:661871/1‑141 (MQ=255)
                                                                                                           ttAACGCCATCAATTTATTACTTTCTACAAAACATTCTATTACAACTGTTTCTGAATTGGCCGGTTTTAACAGTCATACAAATTTTGCAAATCAATTTAAGAATTACTTGCATTTTAGTCCTAAACAGTTCCGTTCACTCg                                  <  2:90380/141‑1 (MQ=255)
                                                                                                              aCGCCATCAATTTATTACTTTCTACAAAACATTCTATTACAACTGTTTCTGAATTGGCCGGTTTTAACAGTCATACAAATTTTGCAAATCAATTTAAGAATTACTTGCATTTTAGTCCTAAACAGTTCCGTTCACTCGttt                               >  1:94058/1‑141 (MQ=255)
                                                                                                               cGCCATCAATTTATTACTTTCTACAAAACATTCTATTACAACTGTTTCTGAATTGGCCGGTTTTAACAGTCATACAAATTTTGCAAATCAATTTAAGAATTACTTGCATTTTAGTCCTAAACAGTTCCGTTCACTCGTTTc                              >  2:10366/1‑141 (MQ=255)
                                                                                                               cGCCATCAATTCATTACTTTCTACAAAACATTCTATTACAACTGTTTCTGAATTGGCCGGTTTTAACAGTCATACAAATTTTGCAAATCAATTTAAGAATTACTTGCATTTTAGTCCTAAACAGTTCCGTTCACTCGTTTc                              >  1:291582/1‑141 (MQ=255)
                                                                                                                   aTCAATTTATTACTTTCTACAAAACATTCTATTACAACTGTTTCTGAATTGGCCGGTTTTAACAGTCATACAAATTTTGCAAATCAATTTAAGAATTACTTGCATTTTAGTCCTAAACAGTTCCGTTCACTCGTTTCCAAg                          >  2:667145/1‑141 (MQ=255)
                                                                                                                       aTTTATTACTTTCTACAAAACATTGTATTACAACTGTTTCTGAATTGGCCGGTTTTAACAGTCATACAAATTTTGCAAATCAATTTAAGAATTACTTGCATTTTAGTCCTAAACAGTTCCGTTCACTCGTTTCCAAGATTa                      >  2:320663/1‑141 (MQ=255)
                                                                                                                       aTTTATTACTTTCTACAAAACATTCTATTACAACTGTTTCTGAATTGGCCGGTTTTAACAGTCATACAAATTTTGCGAATCAATTTAAGAATTACTTGCATTTTAGTCCTAAACAGTTCCGTTCACCCGTTTCCAAGATTa                      <  1:363289/141‑1 (MQ=255)
                                                                                                                       aTTTATTACTTTCTACAAAACATTCTATTACAACTGTTTCTGAATTGGCCGGTTTTAACAGTCATACAAATTTTGCAAATCAATTTAAGAATTACTTGCATTTTAGTCCTAAACAGTTCCGTTCACTCGTTTCCAAGATTa                      <  1:70303/141‑1 (MQ=255)
                                                                                                                       aTTTATTACTTTCTACAAAACATTCTATTACAACTGTTTCTGAATTGGCCGGTTTTAACAGTCATACAAATTTTGCAAATCAATTTAAGAATTACTTGCATTTTAGTCCTAAACAGTTCCGTTCACTCGTTTCCAAGATTa                      <  1:70543/141‑1 (MQ=255)
                                                                                                                       aTTTATTACTTTCTACAAAACATTCTATTACAACTGTTTCTGAATTGGCCGGTTTTAACAGTCATACAAATTTTGCAAATCAATTTAAGAATTACTTGCATTTTAGTCCTAAACAGTTCCGTTCACTCGTTTCCAAGATTa                      <  1:615649/141‑1 (MQ=255)
                                                                                                                       aTTTATTACGTTCTACAAAACATTCTAGTACAACTGTTTCTGAGTTGGCCGGGTTTAACAGTCATACAAATTTTGCAAATCAATTGAAGAATTACTTGCATTTTAGTCCTAAACAGTTCCGTTCACTCGTTTCCAAGATTa                      >  2:686148/1‑141 (MQ=255)
                                                                                                                        tttATTACTTTTGACAAAACATTCTATTACGACTGTTTCTGAATTGGCCGGTTTTAACAGTCATACAAATTTTGCAAATCAATTTAAGAATTACTTGCATTTTAGTCCTAAACAGTTCCGTTCACTCGTTTCCAAGATTAc                     <  2:439852/141‑1 (MQ=255)
                                                                                                                        tttATTACTTTCTACAAAACATTCTATTACAACTGTTTCTGAATTGGCCGGTTTTAACAGTCATACAAATTTTGCAAATCAATTTAAGAATTACTTGCATTTTAGTCCTAAACAGTTCCGTTCACTCGTTTCCAAGATTAc                     <  2:34815/141‑1 (MQ=255)
                                                                                                                          tattaCTTTCTACAAAACATTCTATTACAACTGTTTCTGAATTGGCCGGTTTTAACAGTCATACAAATTTTGCAAATCAATTTAAGAATTACTTGCATTTTAGTCCTAAAGAGTTCCGTTCACTCGTTTCCAACATTACTg                   <  2:490016/141‑1 (MQ=255)
                                                                                                                               cTTTCTACAAAACATTCTATTACAACTGTTTCTGAATTGGCCGGTTTTAACAGTCATACAAATTTTGCAAATCAATTTAAGAATTACTTGCATTTTAGTCCTAAACAGTTCCGTTCACTCGTTTCCAAGATTACTGAacca              <  1:168636/141‑1 (MQ=255)
                                                                                                                                 ttCTACAAAACATTCTATTACAACTGTTTCTGAATTGGCCGGTTTTAACAGTCATACAAATTTTGCAAATCAATTTAAGAATTACTTGCATTTTAGTCCTAAACAGTTCCGTTCACTCGTTTCCAAGATTACTGAaccacc            >  1:633544/1‑141 (MQ=255)
                                                                                                                                  tctcCAAAACATTACTTTACAACGATTTCTGAATTGGCCGGTTTTAACAGTCATACAAATTTTGCAAATCAATTTAAGAATTACTTGCATTTTCGTCCTAAACAGTTCCGTTCACTCGTTTCCAAGATTACTGAAccacca           <  2:450106/141‑1 (MQ=255)
                                                                                                                                        aaaCATTCTATTACAACTGTTTCTGAATTGGCCGGTTTTAACAGTCATACAAATTTTGCAAATCAATTTAAGAATTACTTGCATTTTAGTCCTAAACAGTTCCGTTCACTCGTTTCCAAGATTACTGAACCACCACAAATa     <  2:9243/141‑1 (MQ=255)
                                                                                                                                        aaaCATTCTATTACAACTGTTTCTGAATTGGCCGGTTTTAACAGTCATACAAATTTTGCAAATCAATTTAAGAATTACTTGCATTTTAGTCCTAAACAGTTCCGTTCACTCGTTTCCAAGATTACTGAACCACCACAAATa     >  2:32538/1‑141 (MQ=255)
                                                                                                                                        aaaCATTCTATTACAACTGTTTCTGAATTGGCCGGTTTTAACAGTCATACAAATTTTGCAAATCAATTTAAGAATTACTTGCATTTTAGTCCTAAACAGTTCCGTTCACTCGTTTCCAAGATTACTGAACCACCACAAATa     >  1:398409/1‑141 (MQ=255)
                                                                                                                                           cATTCTATTACAACTGTTTCTGAATTGGCCTGTTTTAACAGTCATACAAATTTTGCAAATCAATTTAAGAATTACTTGCATTTTAGTCCTAAACAGTTCCGTTCACTCGTTTCCAAGATTACTGAACCACCACAAATacac  <  2:77247/141‑1 (MQ=255)
                                                                                                                                           cATTCTATTACAACTGTTTCTGAATTGGCCGGTTTTAACAGTCATACAAATTTTGCAAATCAATTTAAGAATTACTTGCATTTTAGTCCTAACCAGTTCCGTTCACTCGTTTCCAAGATTACTGAACCACCACAAATacac  <  2:610184/141‑1 (MQ=255)
                                                                                                                                           cATTCTATTACAACTGTTTCTGAATTGGCCGGTTTTAACAGTCATACAAATTTTGCAAATCAATTTAAGAATTACTTGCATTTTAGTCCTAAACAGTTCCGTTCACTCGTTTCCAAGATTACTGAACCACCACAAATacac  <  2:379373/141‑1 (MQ=255)
                                                                                                                                           cATTCTATTACAACTGTTTCTGAATTGGCCGGTTTTAACAGTCATACAAATTTTGCAAATCAATTTAAGAATTACTTGCATTTTAGTCCTAAACAGTTCCGTTCACTCGTTTCCAAGATTACTGAACCACCACAAATacac  <  2:208560/141‑1 (MQ=255)
                                                                                                                                           cATTCCATTACAACTGTTTCTGAATTGGACGGTTTTAACAGTCATACAAATTTTGCAAATCAATTTAAGAATTACTTGCATTTTAGTCCTAAACAGCTCCGTTCACTCGTTTCCAAGATTACTGAACCACCACAAATacac  <  1:394219/141‑1 (MQ=255)
                                                                                                                                           cATCCTATTACAACTGTTTCTGAATTGGCCGGTTTTAACAGTCATACAAATATTGCAAATCAATTTAAGAATTACTTGCATTTTAGTACTAAACAGTTCCGTCCACTAGCATTCCACAGTACTGCACCACCACACATacac  >  2:283127/1‑141 (MQ=255)
                                                                                                                                            |                                                                                                                                           
AAAGATATAGCGATGCATTGCAACATCTCTGAATCATATTGTTCTAATCTTTTTGTTCGATATTTGAGTATGAATTTTAAAGATTATTTTACAAGTATTAAACTTGTTAACGCCATCAATTTATTACTTTCTACAAAACATTCTATTACAACTGTTTCTGAATTGGCCGGTTTTAACAGTCATACAAATTTTGCAAATCAATTTAAGAATTACTTGCATTTTAGTCCTAAACAGTTCCGTTCACTCGTTTCCAAGATTACTGAACCACCACAAATACAC  >  CP000730/2501975‑2502253

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: