BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP000730	2,841,601	C→T	11.9%	D170N (GAC→AAC)	USA300HOU_2683 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	2,841,601	0	C	T	11.9%	228.3 / 4.3	100	D170N (GAC→AAC)	USA300HOU_2683	hypothetical protein
Reads supporting (aligned to +/- strand): ref base C (49/37); new base T (11/3); total (60/40)
Fisher's exact test for biased strand distribution p-value = 1.52e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01

CTAATACGCCCTCATAACCGGTTAAAGCAACAACACCTTTTTCACCATTTAAAGAAAAATCTGGATGCTCTTTTACAAATTTATTTAAAATTGGCACGATATCATTGTCATCAGAATAAGTAGCATGGCCTTTTTTGTCTGTAGTTTCAGAAACAACATGTTTATTTTTATCGAGTACTAAACGGTCAGCATAACCATGGTGTCTCATGTAACTATAGTAATTCATATCATCAATTGAGATGATTAGTGGCTTTTTACCTTTCGGCAATTTTATTTTT  >  CP000730/2841463‑2841740

gtaatccggcccaaTAACCGGTAACAGCAACAAGGCCTGTTTCACCATGTAAAGGAAAATCGGGATGCTCTTTTACAAATTTAATGAAAATTGGCACGATATCATTGTCATCAGAATAAGTAGCATGGCCTTTTTTGTCTg                                                                                                                                           <  1:662366/128‑1 (MQ=255)

  aaTACGCCCTCATAACCGGTTAAAGCAACAACACCTTTTTCACCATTTAAAGAAAAATCTGGATGCTCTTTTACAAATTTATTTAAAATTGGCACGATATCATTGTCATCAGAATAAGTAGCATGGCCTTTTTTGTCTGTa                                                                                                                                         <  2:190987/141‑1 (MQ=255)

   aTACGCCCTCATAACCGGTTAAAGCAACAACACCTTTTTCACCATTTAAAGAAAAATCTGGATGCTCTTTTACAAATTTATTTAAAATTGGCACGATATCATTGTCATCAGAATAAGTAGCATGGCCTTTTTTGTCTGTAg                                                                                                                                        <  1:558785/141‑1 (MQ=255)

    tACGCCCTCATAACCGGTTAAAGCAACAACACCTTTTTTTTTTTTTTTTGAAAAATCTGGAGCCTCACTAACTAATTTTTTTTAAATTGGCTCGATATCGTTGTCCTCAGCATAAGTACCATGGCCTATCTTGTTTGTact                                                                                                                                       >  2:19008/1‑139 (MQ=255)

    tACGCCCTCATAACCGGTTAAAGCAACAACACCTTTTTCACCATTTAAAGAAAAATCTGGATGCTCTTTTACAAATTTATTTAAAATTGGCACGATTTCATTGTCATCAGAATAAGTAGCATGGCCTTTTTTTGCTGTAGt                                                                                                                                       >  2:295869/1‑141 (MQ=255)

      cGCCCTCATAACCGGTTAAAGCAACAACACCTTTTTCACCATTTAAAGAAAAATCTGGATGCTCTTTTACAAATTTATTTAAAATTGGCACGATATCATTGTCATCAGAATAAGTAGCATGGCCTTTTTTGTCTGTAGttt                                                                                                                                     >  1:188933/1‑141 (MQ=255)

       gCCCTCATAACCGGTTAAAGCAACAACAGCTTTTTCACCATTTAAAGAAAAATCCGGATGCTCTTTTACAAATTTATTTAAAATTGGCACGATATCATTGTCATCAGAATAAGTAGCATGGCCTTTTTTGTCTGTAGTTTc                                                                                                                                    >  1:448735/1‑141 (MQ=255)

       gCCCTCATAACCGGTTAAAGCAACAACACCTTTTTCACCATTTAAAGAAAAATCTGGATGCTCTTTTACAAATTTATTTAAAATTGGCACGATATCATTGTCATCAGAATAAGTAGCATGGGCGTTTTTGTCTGttggttt                                                                                                                                    >  1:375034/1‑135 (MQ=255)

       gCCCTCATAACCGGTTAAAGCAACAACACCTTTTTCACCATTTAAAGAAAAATCTGGATGCTCTTTTACAAATTTATTTAAAATTGGCACGATATCATTGTCATCAGAATAAGTAGCATGGCGTTTTGTGTCTGTAGTTTc                                                                                                                                    <  2:79066/141‑1 (MQ=255)

       gCCCTCATAACCGGTTAAAGCAACAACACCTTTTTCACCATTTAAAGAAAAATCTGGATGCTCTTTTACAAATTTATTTAAAATTGGCACGATATCATTGTCATCAGAATAAGTAGCATGGCCTTTTTTGTCTGTAGtttt                                                                                                                                    >  1:375062/1‑140 (MQ=255)

       gCCCTCATAACCGGTTAAAGCAACAACACCTTTTTCACCATTTAAAGAAAAATCTGGATGCTCTTTTACAAATTTATTTAAAATTGGCACGATATCATTGTCATCAGAATAAGTAGCATGGCCTTTTTTGTCTGTAGTTTc                                                                                                                                    <  2:412060/141‑1 (MQ=255)

       cccttCATAACCTGTTAAAGCAACAACTCCTTTTTCCCCTTTTAAAGTTATATCTGGATGCTCTTTTACAAATTTATTTAAAATTGGCACGATATCATTGTCATCTGAATATGTAGCATGGTCTTTTTTGTTTGTAGTTTc                                                                                                                                    <  2:589509/137‑1 (MQ=255)

         ccTCATAACCGGTTAAAGCGACAACACCTTTTTCACCATTTAAAGAAAAATCTGGATGCGCTTTTACAAATTTATTTAAAATTGGCACGATATCATTGTCATCAGAACAAGTAGCATGGCCTTTTTTGACTGTAGTTTCAg                                                                                                                                  >  2:388845/1‑141 (MQ=255)

         ccTCATAACCGGTTAAAGCAACAACACCTTTTTTACCTTTTAAAGAAAAATCTGGATGCTCTTTTACAAATTTATTTAAAATTGGCACGATATCATTGTCATCAGCATAAGTAGCATGGCCTTTTTTTTCTGTAGTTTCAg                                                                                                                                  >  1:658728/1‑141 (MQ=255)

         ccTCATAACCGGTTAAAGCAACAACACCTTTTTCACCATTTAAAGAAAAATCTGGATGCTCTTTTACAAATTTATTTAAAATTGGCACGATATCATTGTCATCAGAATAAGTAGCCTGGCCTTTTGTGTCTGTAGTTTCAg                                                                                                                                  >  1:69748/1‑141 (MQ=255)

         ccTCATAACCGGTTAAAGCAACAACACCTTTTTCACCATTTAAAGAAAAATCTGGATGCTCTTTTACAAATTTATTTAAAATTGGCACGATATCATTGTCATCAGAATAAGTAGCATGGCCTTTTTTGTCTGTATTTTTAg                                                                                                                                  >  1:312679/1‑141 (MQ=255)

         ccTCATAACCGGTTAAAGCAACAACACCTTTTTCACCATTTAAAGAAAAATCTGGATGCTCTTTTACAAATTTATTTAAAATTGGCACGATATCATTGTCATCAGAATAAGTAGCATGGCCTTTTTTGTCTGTATTTTCAg                                                                                                                                  >  2:656298/1‑141 (MQ=255)

            cATAACCGGTTAAAGCAACAACCCCTTTTTCACCATTTAAAGAAAAATCTGGATGCTCTTTTACAAATTTATTTAAAATTGGCACGATATCATTGTCATCAGAATAAGTAGCATGGCCTTTTTTGTCTGTAGTTTCAGaaa                                                                                                                               <  1:596440/141‑1 (MQ=255)

            cATAACCGGTTAAAGCAACAACACCTTTTTCTCTTTTTAAAGAAAAATCTGGATGCTCTTTTACAAATTTATTTAAAATTGGCACGATATCATTGTCTTCAGAATAAGTAGCATCGCCTTTTTTGTCTTTAGTTTCAaaaa                                                                                                                               >  2:362170/1‑141 (MQ=255)

            cATAACCGGTTAAAGCAACAACACCTTTTTCACCATTTAAAGAAAAATCTGGATGCTCTTTTACAAATTTATTTAAACTTGGCAAGATATCATTGTCATCATAATAAGTAGCATGGCCTTTTTTGTCTGTAGTTTCAGaaa                                                                                                                               >  1:679896/1‑141 (MQ=255)

            cATAACCGGTTAAAGCAACAACACCTTTTTCACCATTTAAAGAAAAATCTGGATGCTCTTTTACAAATTTATTTAAAATTGGCACGATATCATTGTCATCAGAATAAGTAGCATGGCCTTTTTTGTCTGTAGTTTCAGaga                                                                                                                               >  1:86761/1‑139 (MQ=255)

            cATAACCGGTTAAAGCAACAACACCTTTTTCACCATTTAAAGAAAAATCTGGATGCTCTTTTACAAATTTATTTAAAATTGGCACGATATCATTGTCATCAGAATAAGTAGCATGGCCTTTTTTGTCTGTAGTTTCAGaaa                                                                                                                               <  2:122085/141‑1 (MQ=255)

            cATAACCGGTTAAAGCAACAACACCTTTTTCACCATTTAAAGAAAAATCTGGATGCTCTTTTACAAATTTATTTAAAATTGGCACGATATCATTGTCATCAGAATAAGTAGCATGGCCTTTTTTGTCTGTAGTTTCAGaaa                                                                                                                               <  1:656721/141‑1 (MQ=255)

             aTAGCCGGTTAAAGCAACAACACCTTTTTCACCATTTAAAGAAAAATCTGGATGCTCTTTTACAAATTTATTTAAAATTGGCACGATATCATTGTCATCAGAATAAGTAGCATGGCCTTTTTTGTCTGTAGTTTCAGAaac                                                                                                                              <  1:260976/141‑1 (MQ=255)

             aTAACCGGTTAAAGCAACAACACCTTTTTCACCATTTAAAGAAAAATCTGGATGCTCTTTTACAAATTTATTTAAAATTGGCACGATATCATTGTCATCAGAATAAGTAGCATGGCCTTTTTTGTCTTTTGTTTCAGaaaa                                                                                                                              >  2:107303/1‑140 (MQ=255)

               aaCCGGTTAAAGCAACAACACCTTTTTCACCATTTAAAGAAAAATCTGGATGCTCTTTTACAAATTTATTTAAAATTGGCACGATATAATTGTTATCTGAATAAGTAGCATGGCCTTTTTTGACTGTTGTTTTagaaagaa                                                                                                                            >  1:142211/1‑141 (MQ=255)

                  cGGTTAAAGCAACAACACCTTTTTCACCATTTAAAGAAAAATCTGGATGCTCTTTTACAAATTTATTTAAAATTGGCACGATATCATTGTCATCAGAATAAGTAGCATGGCCTTTTTTGTTTGTAGTTTCAGTAATAACAt                                                                                                                         >  1:256821/1‑141 (MQ=255)

                      tAAAGCAACAACCTCTTTTTCACCATTTAAAGAAAAATCTTGATGCTCTTTTACAAATTTATTTAAAATTGGCACGATATCATTTTCATCAGAATAAGTAGCATGGCCTTTTTTGTCTGTAGTTTCAGAAACAACATGttt                                                                                                                     <  2:158397/141‑1 (MQ=255)

                         aGCAACAACACCTTTTTCACCATTTAAAGAAAAATCTGGATGCTCTTTTACAAATTTATTTAAAATTGGCACGATATCATTGTCATCAGAATAAGTAGCATGGCCTTTTTTGTCTGTAGTTTCAGAACCAACATGTTTAtt                                                                                                                  >  1:208315/1‑141 (MQ=255)

                         aGCAACAACACCTTTTTCACCATTTAAAGAAAAATCTGGATGCTCTTTTACAAATTTATTTAAAATTGGCACGATATCATTGTCATCAGAATAAGTAGCATGGCCTTTTTTGTCTGTAGTTTCAGAAACAACATGTTTAtt                                                                                                                  >  1:55971/1‑141 (MQ=255)

                         aGCAACAACACCTTTTTCACCATTTAAAGAAAAATCTGGATGCTCTTTTACAAATTTATTTAAAATTGGCACGATATCATTGTCATCAGAATAAGTAGCATGGCCTTTTTTGTCTGTAGTTTCAGAAACAACATGTTTAtt                                                                                                                  <  2:452253/141‑1 (MQ=255)

                         aGCAACAACACCTTTTTCACCATTTAAAGAAAAATCTGGATGCTCTTTTACAAATTTATTTAAAATTGGCACGATATAATTTTCATTAGAATAAGTAGCATGGCCTTTTTTGTCTGTAGTTTCAGAATCAAAATAGTTAtt                                                                                                                  >  1:336380/1‑141 (MQ=255)

                          gCAACAACACCTTTTTCACCATTTAAAGAAAAATCTGGATGCTCTTTTACAAATTTATTTAAAATTGGCACGATATCATTGTCATCAGAATAAGTAGCATGGCCTTTTTTGTCTGTAGTTTCAGAAACAACATGGTTAttt                                                                                                                 >  1:41775/1‑141 (MQ=255)

                            aacaacACCTTTTTCACCATTTAAAGAAAAATCTGGATGCTCTTTTACAAATTTATTTAAAATTGGCACGATATCATTGTCATCAGAATAAGTAGCATGGCCTTTTTTGTCTGTAGTTTCAGAAACAACATGTTTAttttt                                                                                                               <  1:112555/141‑1 (MQ=255)

                             acaacaCCTTTTTCCCCATGTAAAGAAAAATCTGGATGCTCTTTTACAAATTTATTTAAAATTGGCACGATATCATTGTCATCAGAATAAGTAGCATGGCCTTTTTTGTCTGTATTTTCAGAAACAACATGTTTAtttttt                                                                                                              >  1:383158/1‑140 (MQ=255)

                             acaacaCCTTTTTCACCATTTAAAGAAAAATCTGGATGCTCTTTTACAAATTTATTTAAAATTGGCACGATATCATTGTCATCAGAATAAGTAGCATGGCCTTTTTTGTTTTTTTTTTCAGAAACAACATGTTTAtttttt                                                                                                              >  2:398438/1‑140 (MQ=255)

                             acaacaCCTTTTTCACCATTTAAAGAAAAATCTGGATGCTCTTTTACAAATTTATTTAAAATTGGCACGATATCATTGTCATCAGAATAAGTAGCATGGCCTTTTTTGTCTGTAGTTCCAGAAACAACATGTTTAttttat                                                                                                              >  1:461574/1‑139 (MQ=255)

                             acaacaCCTTTTTCACCATCTAAAGAAAAATCTGGATGCTCTTTTACAAATTTATTTAAAATTGGCACGATATCATTGTCATCAGAATAAGTAGCATGGCCTTTTTTGTCTGTAGTTTCAGAAACAACATGTTTAtttttt                                                                                                              >  1:383172/1‑140 (MQ=255)

                                 cacCTTTTTCACCATTTAAAGAAAAATCTGGATGCTCTTTTACAAATTTATTTAAAATTGGCACGATATCATTGTCATCAGAATAAGTAGCATGGCCGTTTTTGTCTTTAGTTTCAGAAACAACATATTTATTTTTATcca                                                                                                          >  1:102338/1‑139 (MQ=255)

                                   ccTTTTTCACCATTTAAAGAAAAATCTGGATGCTCTTTTACAAATTTATTTAAAATTGGCACGATATCATTGTCATCAGAATAAGTAGCATGGCCTTTTTTTTCTTTTTTTTCAGAAACAACATGTTTATTTTTATCGAga                                                                                                        >  2:470480/1‑140 (MQ=255)

                                       tttCACCATTTAAAGAAAAATCTGGATGCTCTTTTACAAATTTATTTAAAATTGGCACGATATCATTGTCATCAGAATAAGTAGCATGGCCTTTTTTGTCTGTAGTTTCAGAAACAACATGTTTATTTTTATCGAGTACTa                                                                                                    >  2:401372/1‑141 (MQ=255)

                                          cACCATTTAAAGAAAAATCTGGATGCTCTTTTACAAATTTATTTAAAATTGGCACGATATCATTGTCATCAGAATAACTAGCATGGCCTTTTTTGTCTGTAGTTTCAGAAACAACACGTTTATTGCTATCGAGAAcacaac                                                                                                 >  1:155568/1‑136 (MQ=255)

                                          cACCATTTAAAGAAAAAGCTGGATGCTCTTTTACAAATTTATTTAAAATTGGCACGAGATCATTGTCATCAGAATAAGTAGCATGGCCTTTTTTGTCTGTAGTTTCAGAAACAACATGTTTATTTTTATCGAGTACTAAAc                                                                                                 >  2:610243/1‑141 (MQ=255)

                                           aCCATTTAAAGAAAAATCTGGATGCTCTTTTACAAATTTATTTAAAATTGGCACGATATCATTGTCATCAGAATAAGTAGCATGGCCTTTTTTGTCTGTAGTTTCAGAAACAACATGTTTATTTTTATCGAGTACTAAACg                                                                                                >  1:668898/1‑141 (MQ=255)

                                                  aaaGAAAAATCTGGATGCTCTTTTACAAATTTATTTAAAATTGGCACGATATCATTGTCATCAGAATAAGTAGCATGGCCTTTTTTTTTTTTTTTTTCAGAAACAACATGTTTATTTTTATCGAGTAATACACGGTCGGct                                                                                         >  2:56025/1‑140 (MQ=255)

                                                    aGAAAAATCTGGATGCTCTTTTACAAATTTATTTAAAATTGGCACGATATCATTGTCATCAGAATAAGTAGCATGGCCTTTTTTGTCTGTAGTTTCAGAAACAACATGTTTATTTTTATCGAGTACTAAACGGTCAGCATa                                                                                       >  2:379445/1‑141 (MQ=255)

                                                              ggATTCTCTTTTACAAATTTATTTAAAATTGGCACGATATCATTGTCATCAGAATAAGTAGCATGGCCTTTTTTGTCTGTAGTTTCAGAAACAACATGTTTATTTTTATCGAGTACTAAACGGTCAGCATAACCATGgtgt                                                                             <  1:95876/141‑1 (MQ=255)

                                                              ggATGCTCTTTTACTAATTTATGTTAAATTGTCACGGTATCATTGTCATCAGAATAAGTAGCATGGCCTTTTTTGTCTGTAGTTTCAGAAACAACATGTTTATTTTTATCGAGTACTAAACGGTCAGCATAACCATGgtgt                                                                             >  2:406866/1‑141 (MQ=255)

                                                              ggATGCTCTTTTACAAATTTATTTAAAATTGGCACGATATCATTGTCATCAGAATAAGTAGCATGGCCTTTTTTTTTTTTTTTTTATTAAACAACATGTGTATTTTTATCGAGTACTCAACGGGCTGCCTAACCATGgtgt                                                                             >  2:256899/1‑141 (MQ=255)

                                                              ggATGCTCTTTTACAAATTTATTTAAAATTGGCACGATATCATTGTCATCAGAATAAGTAGCATGGCCTTTTTTGTCTGTAGTTTCAGAAACAACATGTTTATTTTTATCGAGTACTAAACGGTCAGCATAACCATGgtgt                                                                             >  1:78188/1‑141 (MQ=255)

                                                              ggATGCTCTTTTACAAATTTATTTAAAATTGGCACGATATCATTGTCATCAGAATAAGTAGCATGGCCTTTTTTGTCTGTAGTTTCAGAAACAACATGTTTATTTTTATAGAGTAGTAAACGGTTAGCATAATAATGgtgt                                                                             >  1:296774/1‑141 (MQ=255)

                                                               gATGCTCTTTTACAAATTTATTTAAAATTGGCACGATATCATTGTCATCAGAATAAGTAGCATGGCCTTTTTTGTCTGTAGTTTCAGAAACAACATGTTTATTTTTATCGAGTACTAAACGGTCAGCATAACCATGGTGtc                                                                            <  1:256510/141‑1 (MQ=255)

                                                               gATGCTCTTTTACAAATTTATTTAAAATTGGCACGATATCATTGTCATCAGAATAAGTAGCATGGCCTTTTTTGTCTGTAGTTTCAGAAACAACATGTTTATTTTTATCGAGTACTAAACGGTCAGCATAACCATGGTGtc                                                                            <  2:429054/141‑1 (MQ=255)

                                                                aTGTTCTTTTACAAATTTATTGAAAATTGTCACGATATCATGGTCATCAGAATAAGTAGCATGGCCTTTTTTGTCTGTAGTTTCAGAAACAACATGTTTATTTTTATCGAGTACTAAACGGTCAGCATAACCATGGTGtct                                                                           <  2:16594/141‑1 (MQ=255)

                                                                aTGCTCTTTTACAAATTTATTTAAAATTGGCACGATATCATTGTCATCAAAATAAGTAGCATGTTCTGTAGTGCCTGTAGTTTCAGAACTAACATGTTTATTTTTATCGGGTACTAAACGGTAAGCATAACCATGGTGtct                                                                           >  2:105/1‑141 (MQ=255)

                                                                 tGCTCTTTTACAAATTTATTTAAAATTGGCACGATATCATTGTCATCAGAATAAGTAGCATGGCCTTTTTTGTCTGTAGTTTCAGAAACAACATGTTTATTTTTATCGAGTACTAAACGGTCAGCATAACCATGGTGtctc                                                                          <  2:188933/141‑1 (MQ=255)

                                                                 tGCTCTTTTACAAATTTATTTAAAATTGGCACGATATCATTGTCATCAGAATAAGTAGCATGGCCTTTTTTGTCTGTAGTTTCAGAAACAACATCTTTATTTTTATCGAGTACTAAACGGCCACCATAACCAAGGTGtctc                                                                          >  1:176635/1‑141 (MQ=255)

                                                                 tGCTCTTCTACAAATTTATTTAAAATTGGCACGATATCATTGTCATCAGAATAAGTAGCATGGCCTTTTTTGTCTGTAGTTTCAGAAACAACATGTTTATTTTTATCGAGTACTAAACGGTCAGCATAACCATGGTGtctc                                                                          <  1:41823/141‑1 (MQ=255)

                                                                   ctctTTTACAAATTTATTTAAAATTGTCACGATATCATTGTCATCAGAATAAGTAGCATGGCCTTTTTTGTCTGTAGTTTCATAAACAACATGTTTATTTTTATCGAGTACTAAACGGTCAGCATAACCATGGTGTCTCAt                                                                        <  1:420099/141‑1 (MQ=255)

                                                                   ctctTTTACAAATTTATTTAAAATTGGCACGATATCATTGTCATCAGAATAAGTAGCATGGCCTGTTTTGTCTGTAGTTTCAGAAACAACATGTTTATTTTTATCGAGTACTAAACGGTCATCATAACCACGGTGTCTCac                                                                        >  2:584857/1‑140 (MQ=255)

                                                                   ctctTTTACAAATTTATTTAAAATGGGCACGATATCATTGTCATCAGAATAAGTAGCATGGCCTTTTTTGTCTGTAGTTTCAGAAACAACATGTTTATTTTTATCGAGTACTAAACGGTCAGCATAACCATGGTGTCTCAt                                                                        <  2:590266/141‑1 (MQ=255)

                                                                   ctctTTTACAAATTAATTTAAAATTGGCACGATATCATTGTCATCAGAATAAGTAGCATGGCCTTTTTTGTCCGTAGTTTCAGAAACAACATGTTTATTTTTATCGAGTACTAAACGGTCAGCATAACCATGGTGTCTCAt                                                                        >  2:471485/1‑141 (MQ=255)

                                                                      ttttACAAATTTATTTAAAATTGGCACGATATCATTGTCATCAGAATAAGTAGCATGGCCTTTTTTGTCTGTAGTTTCAGAAACAACATGTTTATTTTTATCGAGTACTAAACGGTCAGCATAACCATGGTGTCTCATGTa                                                                     <  1:401372/141‑1 (MQ=255)

                                                                          aCAAATTTATTTAAAATTGGCACGATATCATTGTCATCAGAATAAGTAGCATGGCCTTTTTTGTCTTTAGTTTCAGAAACAACATGTTTATTTTTATCGATTACTAAACGGTCAGCCTAACCATGGTGTCTCATGTAACtt                                                                 >  2:627676/1‑140 (MQ=255)

                                                                          aCAAATTTATTTAAAATTCGCACGTTATCATTGTCATCAGAATAAGTAGCATGGCCTTTTTTGTCTGTAGTTTCAGAAACAACATGTTTATTTTTATCGAGTACTAAACGGTCAGCATAACCATGGTGTCTCATGTAACta                                                                 <  2:44183/141‑1 (MQ=255)

                                                                           cAAATTTATTTAAAATTGGCAAGATATCATTGTCAGCAGAATAAGTAGCATGGCCTTTTTTGTTTGTAGTTTCAGAAACAACATTTTTATTTTTATCGAGTACTAAACGGTCAGCATAACCATGGTGTCTCATGTAACtat                                                                <  1:170663/141‑1 (MQ=255)

                                                                              atttatttAAAATTGGCACGATATCATTGTCATCAGAATAAGTAGCATGGCCTTTTTTGTCTGTAGTTTCAGAAACAACATGTTTATTTTTATCGAGTACTAAACGGTCAGCATAACCATGGTGTCTCATGTAACTATAGt                                                             >  2:235367/1‑141 (MQ=255)

                                                                                     taAAATTGGCACGATATCATTGTCATCAGAATACGTAGCATGGCCTTTTTTGTTTGTAGTTTACGAAACAACATATTTATTTTTCTCGAGTACTAAGCGGTCAGCATAACCGTGGTGTCCCATATAGCTCTAGTAAATCat                                                      >  1:317848/1‑141 (MQ=255)

                                                                                          ttGGCACGATATCATTGTCATCAGAATAAGTAGCATGGCCTTTTTTGTCTGTAGTTTCAGAAACAACATGTTTATTTTTATCGAGTACTAAACGGTCAGCATAACCATGGTGTCTCATGTAACTATAGTAATTCATatcat                                                 <  1:64110/141‑1 (MQ=255)

                                                                                          ttGGCACGATACCATTNTCATCAGAATAATTAGCATGGCCTTTTTTGTCTGTAGTTTCAGAAACAACATGTTTATTTTTATCGAGTACTAAACGGTCAGCATAACCATGGTGTCTCATGTAACTATAGTAATTCATatcat                                                 <  1:678290/141‑1 (MQ=255)

                                                                                            ggCACGATATCATTGTCATCAGAATAAGTAGCATGGCCTTTTTTGTCTGTAGTTTCAGAAACAACATGTTTATTTTTATCGAGTACTAAACGGTCAGCATAACCATGGTGTCTCATGTAACTATAGTAATTCATAtcatca                                               >  1:93038/1‑141 (MQ=255)

                                                                                              cACGATATCATTGTCATCAGTATGATTAGCATGGCCTTTTTTGTCTGTAGTTTCAGAAACAACATGTTTATTTTTATCGAGTACTAAACGGTCAGCATAACCATGGTGTCTCATGTAACTATAGTAATTCATATCATCAAt                                             <  2:313743/141‑1 (MQ=255)

                                                                                              cACGATATCATTGTCATCAGAATAAGTAGCATGGCCTTTTTTGTCTGTAGTTTCAGAAACAACATGTTTATTTTTATCGAGTACTAAACGGTCAGCATAACCATGGTGTCTCATGTAACTATAGTAATTCATATCATCAAt                                             <  1:665393/141‑1 (MQ=255)

                                                                                              cACGATATCATTGTCATCAGAATAAGTAGCATGGCCTTTTTTGTCTGTAGTTTAACTATCAACAGGTTTATTTTTATCGAGTACTAAACGGTCAGCATAACCATGGTGTCTCATGTAACTATAGTAATTCATATCATCAAt                                             <  1:679691/141‑1 (MQ=255)

                                                                                               aCGATATCATTGTCATCAGAATAAGTAGCATGGCCTTTTTTGTCTGTAGTTTCAGAAACAACATGTTTATTTTTATCGAGTACTAAACGGTCAGCATAACCATGGTGTCTCATGTAACTATAGTAATTCATATCATCAAtt                                            >  2:247402/1‑141 (MQ=255)

                                                                                                cGATATCATTGTCATCAGAATAAGTAGCATGGCCTTTTTTTTTTTTTTTTTCACAAACAACAGGCTTATTTTTATCGACTACTAAACGGTCAGCATAACCAGGGTGTCTCATGTAACTATAGTAATGCATATCATCAATTg                                           >  2:68027/1‑141 (MQ=255)

                                                                                                 gATATCATTGTCATCAGAATAAGGATCAGGTCCTTTTTTGTCTGTAGTTTCAGAAACAACATGTTTATTTTTATCGAGTACTAAACGGTCAGCATAACCATGGTGTCTCATGTAACTATAGTAATTCATATCATCAATTga                                          <  2:518253/141‑1 (MQ=255)

                                                                                                 gATATCATCGTCATCAGAATAAGTAGCATGGCCTTTGTTCTCTGTAGTTTCAGAAACAACATGTTTATTTTTATCGAGTACTAAACGGTCAGCATAACCATGGTGTCTCATGTAACTATAGTAATTCATCTCATCAATTga                                          <  1:180375/141‑1 (MQ=255)

                                                                                                   tatCATTGTCATCAGAATAAGTAGCATGGCCTTTTTTGTCTGTAGTTTCAGAAACAACATGTTTATTTTTATCGAGTACTAAACGGTCAGCATAACCATGGTGTCTCATGTAACTATAGTAATTCATATCATCAATTgagg                                        >  1:130348/1‑140 (MQ=255)

                                                                                                   tatCATTGTCATCAGAATAAGTAGCATGGCCTTTTTTGTCTGTAGTTTCAGAAACAACATGTTTATTTTTATCGAGTACTAAACGGTCAGCATAACCATGGTGTCTCATGTAACTATAGTAATTCATATCATCAATTgaga                                        >  2:391346/1‑141 (MQ=255)

                                                                                                   tatCATTGTCATCAGAATAAGTAGCATGGCCTTATTTGTCTGTAGTTTCAGAAACAACATGTTTATTTTTATCGAGTACTAAACGGTCAGCATAACCATGGTGTCTCATGTAACTATAGTAATTCATATCATCAATTgaga                                        >  1:610473/1‑141 (MQ=255)

                                                                                                     tCATTGTCACCAGAATAAGTAGCATGGCCTTTTTTTTTTTTTTTTTTAGAAACAACATGTTTATTTTTTTCGATTACTAAACAGTCATCATAACCTTGGTGTCTCCTGTTACTTTATTAATTCCTATCATCAATTgtgctt                                      >  2:553316/1‑136 (MQ=255)

                                                                                                      cATTGTCATCAGAATAAGTAGCATGGCCTTTTTTGTCTGTAGTTTCAGAAACAACATGTTTATTTTTATCGAGTACTAAACGGTCAGCATAACCATGGTGTCTCATGTAACTATAGTAATTCATATCATCAATTGAgatga                                     <  2:55971/141‑1 (MQ=255)

                                                                                                           gcatcaGAATAAGTAGCATGGCCTTTTTTGTTTGTAGTTTCAGAAACAACATGTTTATTTTTATCGAGTATCAAACGGTCAGCATAAGCATGGTGTCTCATGTAACTATAGTAATTCATATCATCAATTGAGATGATTAGt                                <  1:140815/140‑1 (MQ=255)

                                                                                                                aGAATAAGTAGCATGGCCTTTTTTTTTTTTTTTTTCAGAAACAACATGTATATTTTTATCGAGTACTAAACGGTCAGCATAACCATGGTGTCTCCTGTAACTATTGTAATTCATATCATCAATTGAGCTGAtttttgtctt                           >  2:213502/1‑133 (MQ=255)

                                                                                                                aGAATAAGTAGCATGGCCTTTTTTGTCTGTAGTTTCAGAAACAACATGTTTATTTTTATCGAGTACTAAACGGTCAGCATAACCATGGTGTCTCATGTAACTATAGTAATTCATATCATCAATTGAGATGATTAGTGGCtt                           <  1:495462/141‑1 (MQ=255)

                                                                                                                      aGTAGCATGGCCTTTTTTTTTTGTAGTTTCAGAAACAACATGTTTATTTTTATCGAGTACTAAACGGTCAGCATAACCATGGTGTCTCATGTAACTATAGTAAGTCATATCATCAATTGAGATGATTAGTGGCTTTTTAcc                     >  2:554322/1‑141 (MQ=255)

                                                                                                                      aGTAGCATGGCCTTTTTTGTCTGTAGTTTCAGAAACAACATGTTTATTTTTATCGAGTACTAAACGGTCAGCATAACCATGGTGTCTCATGTAACTATAGTAATTCATATCATCAATTGAGATGATTAGTGGCTTTTTAcc                     >  1:413300/1‑141 (MQ=255)

                                                                                                                      aGTAGCATGGCCTTTTTTGTCTGTAGTTTCAGAAACAACATGTTTATTTTTATCGAGTACTAAACGGTCAGCATAACCATGGTGTCTCATGTAACTATAGTAATTCATATCATCAATTGAGATGATTAGTGGCTTTTTAca                     >  1:237876/1‑140 (MQ=255)

                                                                                                                       gTAGCATGGCCTTTTTTGTCTGTAGTTTCAGAAACAACATGTTTATTTTTATCGAGTACTAAACGGTCAGCATAACCATGGTGTCTCATGTAACTATAGTAATTCATATCATCAATTGAGATGATTAGTGGCTTTTTACCt                    >  1:416320/1‑141 (MQ=255)

                                                                                                                        tAGCATGGCCTTTTTTGTCTGTAGTTTCAGAAACAACATGTTTATTTTTATCGAGTACTAAACGGTCAGCATAACCATGGTGTCTCATGTAACTATAGTAATTCATATCATCAATTGAGATGATTAGTGGCTTTTTACCtt                   <  2:448735/141‑1 (MQ=255)

                                                                                                                        tAGCATGGCCTTTTTTGTCTGTAGTTTCAGAAACAACATGTTTATTTTTATCGAGTACTAAACGGTCAGCATAACCATGGTGTCTCATGTAACTATAGTAATTCATATCATCAATTGAGATGATTAGTGGCTTTTTACCtt                   <  2:375062/141‑1 (MQ=255)

                                                                                                                        tAGCATGGCCTTTTTTGTCTGTAGTTTCAGAAACAACATGTTTATTTTTATCGAGTACTAAACGGTCAGCATAACCATGGTGTCTCATGTAACTATAGTAATTCATATCATCAATTGAGATGATTAGTGGCTTTTTACCtt                   <  2:375034/141‑1 (MQ=255)

                                                                                                                         aGCATGGCCTTTTTTTTTTTTTTTTTCAGAAACAACATGTTTATTTTTATCGAGTACTAAACTGTCAGCATAACCATGGTGTCTCATGTAACTATAATAATTAATATCATCAATTTTGATGATTAGTTGCTTCTTACCttt                  >  2:472844/1‑141 (MQ=255)

                                                                                                                         aGCATGGCCTTTTTTGTCTGTAGTTTCAGAAACAACATGTTTATTTTTATCGAGTACTAAACGGTAAGCATAACCATGGTGTCTCATGTAACTATAGTAATTCATATCATCAATTGAGATGGTTAGTGGCTTCTTACCttt                  >  2:472958/1‑141 (MQ=255)

                                                                                                                          gCATGGCCTTTTTTGTCTGTAGTTTCAGAAACAACATGTTTATTTTTATCGAGTACTAAACGGTCAGCATAACCATGGTGTCTCATGTAACTATAGTAATTCATATCATCAATTGAGATGATTAGTGGCTTTTTACCTTTc                 <  1:362170/141‑1 (MQ=255)

                                                                                                                           cATGGCCTTTTTTGTCTGTAGTTTCAGAAACAACATGTTTATTTTTATCGAGTAATAAACGGTCAGCATAACCATGGTGTCTCATGTAACTACAGTAATTCATATCATCAATCGAGATGATTACTGGCTTTTTACCTTCCg                >  2:327219/1‑141 (MQ=255)

                                                                                                                               gCCTTTTTTGTCTGTAGTTTCAGAAACAACATGTTTATTTTTATCGAGTACTAAACGGTCAGCATAACCATGGTGTCTCATGTAACTATAGTAATTCATATCATCAATTGAGATGATTAGTGGCTTTTTACCTTTCGGCaa            >  1:427942/1‑141 (MQ=255)

                                                                                                                                       tGTCTGTAGTTTCAGAAACAACATGTTTATTTTTATCGAGTACTAAACGGTCAGCATAACCATGGTGTCTCATGTAACTATAGTAATTCATATCATCAATTGAGATGATTAGTGGCTTTTTACCTTTCGGCAattttattt    <  1:235367/141‑1 (MQ=255)

                                                                                                                                         tCTGTAGTTTCAGAAACAACATGTTTATTTTTATCGAGTACTAAACGGTCAGCATAACCATGGTGTCTCATGTAACTATAGTAATTCATATCATCAATTGAGATGATTAGTGGCTTTTTACCTTTCGGCAATTTTAttttt  <  1:180054/141‑1 (MQ=255)

CTAATACGCCCTCATAACCGGTTAAAGCAACAACACCTTTTTCACCATTTAAAGAAAAATCTGGATGCTCTTTTACAAATTTATTTAAAATTGGCACGATATCATTGTCATCAGAATAAGTAGCATGGCCTTTTTTGTCTGTAGTTTCAGAAACAACATGTTTATTTTTATCGAGTACTAAACGGTCAGCATAACCATGGTGTCTCATGTAACTATAGTAATTCATATCATCAATTGAGATGATTAGTGGCTTTTTACCTTTCGGCAATTTTATTTTT  >  CP000730/2841463‑2841740

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`