Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 36,133 A→G 31.6% V140A (GTA→GCA)  USA300HOU_0026 ← IS431mec transposase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP00073036,1330AG31.6% 279.4 / 253.8 317V140A (GTA→GCA) USA300HOU_0026IS431mec transposase
Reads supporting (aligned to +/- strand):  ref base A (108/107);  new base G (50/49);  total (160/157)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.76e-01

Only 100 of 320 total aligned reads displayed.

TGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAGCCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCA  >  CP000730/35994‑36273
                                                                                                                                           |                                                                                                                                             
tGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTGAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTAc                                                                                                                                              <  1:73167/141‑1 (MQ=255)
 gcggTAGTGATACTTTGATACCTTGACTTTCTTACTTTAATAGAACGGCGATCTTGCGCAATGAGGTTATTCAGATATTGCGATGTACAATGGCAATCAAGCTTAAGTTTAAAAGCTTTGATTACTTTAACAATTGCTAcc                                                                                                                                             >  2:72735/4‑141 (MQ=14)
 gccgTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGcc                                                                                                                                             >  1:259627/4‑141 (MQ=255)
 gccgTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGcc                                                                                                                                             >  1:397932/4‑141 (MQ=255)
 gCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTCCTAcc                                                                                                                                             >  2:26428/1‑141 (MQ=255)
  cTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAGCCATTGCTACCt                                                                                                                                            <  1:117116/141‑1 (MQ=255)
    gTATTGATACTTTGATATCTTGTCTTTCTTACTTTATTATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAGGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTc                                                                                                                                          <  1:221902/141‑1 (MQ=14)
     tATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATTACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCg                                                                                                                                         >  2:2287/1‑141 (MQ=255)
      aTTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAGCCATTGCTACCTTCGt                                                                                                                                        <  1:210583/141‑1 (MQ=40)
       ttGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGtt                                                                                                                                       >  2:370201/1‑141 (MQ=255)
          aTACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGaa                                                                                                                                    >  2:116435/1‑141 (MQ=255)
            aCTTTGTCACCTTTTCTTTCTTACTTTAATTTGCCGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAgg                                                                                                                                  <  2:60486/141‑1 (MQ=14)
            aCTTTGATACCTTGTCTTTCTTACCTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAgg                                                                                                                                  <  1:156856/141‑1 (MQ=255)
               ttGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGc                                                                                                                               >  1:305808/1‑141 (MQ=255)
               ttGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGc                                                                                                                               <  2:349040/141‑1 (MQ=255)
               ttGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTATAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGc                                                                                                                               <  2:254445/141‑1 (MQ=255)
                tGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAGCCATTGCTACCTTCGTTGAAGGTGcc                                                                                                                              >  1:196622/1‑141 (MQ=38)
                   tACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGa                                                                                                                           >  2:102138/1‑141 (MQ=255)
                    agcTTGTCTCTCTTACTTTAATATGACGGTGATACTGCACGAAGAGGCCAGTAAGCAGTTCCAATGCACAAAGATAGTCACGGTTATATTTGAACGCTTTGATAATTTTAGCCATTGGTTCGTTCGTTGAAGGTGCCTGAt                                                                                                                          >  2:133866/3‑141 (MQ=255)
                      cTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGTTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGctct                                                                                                                        >  1:246926/1‑141 (MQ=255)
                      cTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCt                                                                                                                        <  1:188958/141‑1 (MQ=255)
                        tGTCTTTCTTACTTTAATATGACGGTGATCTCTTTCAATGAGGTTATTCTGTTCTTTCGATGTACAATGGCAGGCAGGTTTAAGTTTAAAAGCTTTAATTACTTTCACCATTGCTACCTTTGTTGAAGTTGCCTGATCTGt                                                                                                                      >  2:281613/1‑141 (MQ=11)
                           ctttcttACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTaac                                                                                                                   >  1:93532/1‑140 (MQ=255)
                            gttcttACTTTAAGATGACGGTGGTCGTGCGCAATGAGGTTATTCAGATATTTGGATGTACAATGACAGTCAGGGTTAAGGTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTaact                                                                                                                  <  2:178610/140‑3 (MQ=11)
                              tcttACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTAc                                                                                                                >  2:341900/1‑141 (MQ=255)
                               cttACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTCATTAcc                                                                                                               >  2:398934/1‑141 (MQ=255)
                               attACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACGTTCGTTGAAGGTGCCTGATCTGTACTTAcc                                                                                                               >  2:166171/2‑141 (MQ=14)
                                ttACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCt                                                                                                              >  1:181127/1‑141 (MQ=255)
                                  aCTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTGCCttt                                                                                                            >  1:381795/1‑141 (MQ=255)
                                    tttAATTTGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTg                                                                                                          <  2:394024/141‑1 (MQ=255)
                                     ttAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGa                                                                                                        <  2:395445/141‑1 (MQ=255)
                                     ttAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGa                                                                                                        >  1:148673/1‑141 (MQ=255)
                                       aaTATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAgg                                                                                                      <  2:51079/141‑1 (MQ=25)
                                         tatGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAggct                                                                                                    >  2:89165/1‑139 (MQ=255)
                                           tGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTAAGGTTTa                                                                                                  >  2:384140/1‑141 (MQ=255)
                                            tcagctGATCTTGCTCAATTATGTTAGTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTCTAATTACCTTTTGAGGTTTAc                                                                                                 <  2:273905/136‑1 (MQ=11)
                                            gACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTAATTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTAc                                                                                                 >  2:298240/1‑141 (MQ=255)
                                            gACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTAc                                                                                                 <  1:316214/141‑1 (MQ=11)
                                             gcGGTGATCTTGCTTAATGATGTTATTCATATATTTCGATGTACACTGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCttttcaagcgtacc                                                                                                 <  2:47245/140‑11 (MQ=11)
                                                gTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTATCTTTTGAGGTTTACCaaa                                                                                             <  2:25025/141‑1 (MQ=12)
                                                  tgactggCTCAATGAGGGTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAAtt                                                                                           <  1:82361/135‑1 (MQ=11)
                                                  gATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTCCCTTTTGAGGTTTACCAAAtt                                                                                           >  2:10635/1‑141 (MQ=12)
                                                    tcgtccttaaTGAGGTTATTTAGATTTTTCGCTGTTCATTGTCTGTCAGGTTTATGTTTTACAGCTTTAACTTCTTTAACCATTGCTACCTTCGTTGCAGGTGTCTGATCTGTAATTACCTTTTGTGGTTTACCAAAtttt                                                                                         <  2:28736/133‑3 (MQ=2)
                                                    tCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTTAAGGTGCCTGATCTGTTATTACCTTTTGAGGTTTACCAATTTGt                                                                                         >  2:332668/1‑141 (MQ=11)
                                                    tCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGt                                                                                         >  2:145747/1‑141 (MQ=17)
                                                    tCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTTATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTTAGGCTTACACAATTGt                                                                                         >  1:102962/1‑141 (MQ=11)
                                                    tCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTCACTACCTTTTGAGGCTGACCagcgtgt                                                                                         >  2:56558/1‑135 (MQ=11)
                                                     cTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGtt                                                                                        >  1:162852/1‑141 (MQ=17)
                                                     cTTGCTAAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGtt                                                                                        >  2:220645/1‑141 (MQ=255)
                                                      ttGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGttt                                                                                       <  1:56143/141‑1 (MQ=17)
                                                      ttGCGCAATGAGGTTATTCAGATACTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGttt                                                                                       <  2:33839/141‑1 (MQ=11)
                                                           cAATGAGGTTCTTCAGATTTTGCGCGCTTCGATGGGAGTCAGGTTTAAGTTTGGAGGGTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATga                                                                                  <  1:52897/141‑1 (MQ=11)
                                                           cAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATga                                                                                  <  2:250904/141‑1 (MQ=12)
                                                           cAATGAGGTTAGTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATga                                                                                  <  2:327302/141‑1 (MQ=11)
                                                           aaaTGTGGTTATTCAGATATTTCGATGTACAATGACAGTCAGTTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGTTGCCTGATCTGTAACTCCTTTTGTATCCTTACCAAATTGTTTAATga                                                                                  <  2:85482/140‑1 (MQ=11)
                                                            aaTGAGGTTATGCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAGCCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATgag                                                                                 <  2:215425/141‑1 (MQ=17)
                                                               gAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAGTTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGCGGTTTACCAAATTGTTTAATGAGCCg                                                                              >  1:243979/1‑141 (MQ=11)
                                                                aGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTCATGAGACGt                                                                             >  1:106989/1‑141 (MQ=11)
                                                                 ggTTATTCAGATATTTCGATGTACAGTGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGtt                                                                            >  1:200519/1‑141 (MQ=12)
                                                                 ggTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGtt                                                                            <  2:190272/141‑1 (MQ=17)
                                                                 ggTTATTAAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGtt                                                                            <  2:318890/141‑1 (MQ=11)
                                                                 ggTTATTAAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGtt                                                                            <  2:307409/141‑1 (MQ=11)
                                                                   ttATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTg                                                                          <  1:353440/141‑1 (MQ=17)
                                                                   ttATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTg                                                                          >  1:392516/1‑141 (MQ=17)
                                                                   ttATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTg                                                                          <  1:46378/141‑1 (MQ=11)
                                                                    tATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTAAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGa                                                                         <  1:205942/141‑1 (MQ=12)
                                                                    tATTCAGATATTTCGATGTACAATGACAGTCAGGTTTACGTTTAAAAGCTTTCATTATTTTAGAAATTGTTAAAGGCGTTGCGGCAGAAGGTTCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGa                                                                         <  1:191450/141‑1 (MQ=2)
                                                                     aTTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTTCTTTAACCATTGCTACCTTCGTTGAAGGATCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGaa                                                                        >  1:172714/1‑140 (MQ=11)
                                                                      ttCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAACTTGTTTAATGAGACGGTTGCTa                                                                       >  2:366764/1‑141 (MQ=11)
                                                                       tCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTTATaa                                                                      >  2:68272/1‑141 (MQ=11)
                                                                         aGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAGCCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAAc                                                                    >  2:23891/1‑141 (MQ=32)
                                                                           atatTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACTAATGTTAACTTCATTTAATGTGCCTGATCTGTAATTACCTTTTGAGGTTTACAAAATTGTTTAATGAGCCGTTTGATAAACGc                                                                  >  2:260413/1‑141 (MQ=11)
                                                                           atatTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGc                                                                  >  1:330742/1‑141 (MQ=17)
                                                                             atTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCat                                                                <  1:325226/141‑1 (MQ=11)
                                                                                tCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATg                                                             >  2:224928/1‑141 (MQ=12)
                                                                                tCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATg                                                             <  1:178690/141‑1 (MQ=17)
                                                                                tCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATg                                                             >  1:289134/1‑141 (MQ=12)
                                                                                tCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGGAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATg                                                             <  2:151083/141‑1 (MQ=11)
                                                                                 cgctGTACAATGACAGTCAGGTCTAAGTTTAAAAGCTTTAATTATTTTAGCCCGTGCTGCCTTCGTTGAAGGTGACTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGGCGTTTGATAACCGccgaggc                                                            >  2:158186/4‑135 (MQ=11)
                                                                                 cGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGc                                                            <  2:43526/141‑1 (MQ=17)
                                                                                  gATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTTAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCt                                                           >  1:19904/1‑141 (MQ=12)
                                                                                  gATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCt                                                           >  1:280942/1‑141 (MQ=11)
                                                                                       aCAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATg                                                      <  1:342934/141‑1 (MQ=17)
                                                                                         aaTGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGAt                                                    <  1:366461/141‑1 (MQ=11)
                                                                                          aTGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTGGAAGGTGCCTGATCTGTAACTACCTTTTGAGGATTACCCAATTCTTTAATGAGAAGTTTGATAAACGCATATGCTGAAGGAtt                                                   <  2:36211/141‑1 (MQ=11)
                                                                                                                               ttAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAatgtatgt              <  2:396487/141‑1 (MQ=14)
                                                                                                                                tcgctaTTGCATCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAACTTGTTGAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTc             <  1:25274/136‑1 (MQ=11)
                                                                                                                                taaccATTGCTACCTTCGTTGAAGGTTCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTc             <  1:26428/138‑1 (MQ=17)
                                                                                                                                 aGCCATTGCTACCTTCGTTGAAGGTGCTTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTcc            <  1:150148/141‑1 (MQ=17)
                                                                                                                                  gCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTccc           >  1:139572/1‑141 (MQ=255)
                                                                                                                                   ccATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCct          >  1:337239/1‑141 (MQ=32)
                                                                                                                                     aTTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCCAATGTCTAATGTATGTTCctct        >  1:354310/1‑141 (MQ=12)
                                                                                                                                     aTTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCctct        >  1:225958/1‑141 (MQ=32)
                                                                                                                                      ttGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTg       >  2:292752/1‑141 (MQ=12)
                                                                                                                                       tGCTACCTTCGTTTAAGGTGCCTGATCTGTAATTATCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGc      <  1:102138/141‑1 (MQ=17)
                                                                                                                                       tGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGc      >  1:372603/1‑141 (MQ=32)
                                                                                                                                         ccaCCTTCGTCGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCACATTGTTTAATGCGACGTTTGATAAACTCAGATGCTGAATTAGTATCTCGTTGCTGACGCAACCAAATATCTAATGTATGTCCCTCTGCAt    <  2:94132/139‑1 (MQ=11)
                                                                                                                                         cTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCAt    >  1:43531/1‑141 (MQ=32)
                                                                                                                                          tACCTTCGATGAAGGTGCCTGATCTTTAATTACCTTTTGATGTTTACCGAATTGTTGAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATc   <  1:110400/141‑1 (MQ=11)
                                                                                                                                           aCCTTCGTTGAAGGTGCCTGATCTGTATTTACCTTTTGAGTTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGATGAATGATTATCTCGTTGCTTAGGCAACCAAATATCTAATGTATGTCCCTCTgtgtca  >  1:351269/1‑136 (MQ=11)
                                                                                                                                           |                                                                                                                                             
TGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAGCCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCA  >  CP000730/35994‑36273

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: