BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP000730	36,174	T→C	29.7%	K126K (AAA→AAG)	USA300HOU_0026 ←	IS431mec transposase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	36,174	0	T	C	29.7%	292.6 / 218.1	316	K126K (AAA→AAG)	USA300HOU_0026	IS431mec transposase
Reads supporting (aligned to +/- strand): ref base T (99/119); new base C (41/52); total (142/174)
Fisher's exact test for biased strand distribution p-value = 9.01e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.04e-01

Only 100 of 321 total aligned reads displayed.

TATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAGCCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGAT  >  CP000730/36035‑36314

tatGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTCAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTTCCTGATCTGTAATTACCTTTTTAGGtt                                                                                                                                             >  2:339983/1‑141 (MQ=11)

  tGACGGTGATCTTGCTCACTTAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCGGTAACTACCTTTTGAGGcttc                                                                                                                                           >  2:385470/1‑140 (MQ=11)

   gACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTAc                                                                                                                                          >  1:63748/1‑141 (MQ=25)

    gcGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTAcc                                                                                                                                         <  1:208477/140‑1 (MQ=19)

    aCGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTAcc                                                                                                                                         >  1:215189/1‑141 (MQ=255)

       gTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCaaa                                                                                                                                      <  2:124344/141‑1 (MQ=17)

        tGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCCTCGGTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAAt                                                                                                                                     <  1:175250/141‑1 (MQ=255)

        tGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGATTAAAAGCTTTCATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGCTTACCAAAt                                                                                                                                     >  2:162073/1‑141 (MQ=11)

         gATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTCCCTTTTGAGGTTTACCAAAtt                                                                                                                                    >  2:10635/1‑141 (MQ=12)

          aTCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTATTGAGGCTTACCAAATTg                                                                                                                                   >  1:397963/1‑141 (MQ=11)

           tCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTTAAGGTGCCTGATCTGTTATTACCTTTTGAGGTTTACCAATTTGt                                                                                                                                  >  2:332668/1‑141 (MQ=11)

           tCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGt                                                                                                                                  >  2:8783/1‑141 (MQ=11)

           tCTTGCTCAATGAAGTTAATCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTTACTATTGCTACCATCGTTGGAGGTGCCTAATGTGTAATTATCTTTTGATGTTTATGAAATAGt                                                                                                                                  >  2:69529/1‑141 (MQ=11)

             ttGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGttt                                                                                                                                >  1:55509/1‑141 (MQ=17)

              tGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTa                                                                                                                               >  1:233521/1‑141 (MQ=255)

               gctgaaggtggTTATTCTGACATTGCTATGGACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTGATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCGGATCTGTAATTACCTTTTGAGGTTTACAAAATGGtgcaa                                                                                                                              <  2:345129/132‑5 (MQ=11)

                ttCAATGGAGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTGCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAAt                                                                                                                             <  2:226175/140‑1 (MQ=11)

                 tCAATGAGGTTATTCAGATATTTCGATGTACACTGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATg                                                                                                                            <  2:217950/141‑1 (MQ=12)

                  cAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATga                                                                                                                           <  2:250904/141‑1 (MQ=12)

                  cAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATga                                                                                                                           <  1:247105/141‑1 (MQ=255)

                  cAATGAGGTTAGTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATga                                                                                                                           <  2:327302/141‑1 (MQ=11)

                       aGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGt                                                                                                                      <  1:353267/141‑1 (MQ=12)

                       aGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGt                                                                                                                      <  2:265845/141‑1 (MQ=17)

                       aGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTCATGAGACGt                                                                                                                      >  1:106989/1‑141 (MQ=11)

                        ggTTATTCAGATTCCGCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAGCCATTGCTACCTTCGTTGAAGATGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGtt                                                                                                                     >  2:185358/1‑141 (MQ=11)

                        ggTTATTCAGATATTTCGATGTACAGTGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGtt                                                                                                                     >  1:200519/1‑141 (MQ=12)

                        ggTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGtt                                                                                                                     <  1:132108/141‑1 (MQ=11)

                        ggTTATTAAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGtt                                                                                                                     <  2:318890/141‑1 (MQ=11)

                        ggTTATTAAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGtt                                                                                                                     <  2:307409/141‑1 (MQ=11)

                         gTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGttt                                                                                                                    <  1:316058/141‑1 (MQ=12)

                          ttATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTg                                                                                                                   >  1:102856/1‑141 (MQ=255)

                           tATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTAAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGa                                                                                                                  <  1:205942/141‑1 (MQ=12)

                           tATTCAGATATTTCGATGTACAATGACAGTCAGGTTTACGTTTAAAAGCTTTCATTATTTTAGAAATTGTTAAAGGCGTTGCGGCAGAAGGTTCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGa                                                                                                                  <  1:191450/141‑1 (MQ=2)

                                aGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAAc                                                                                                             >  2:364654/1‑141 (MQ=12)

                                aGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAGCCCTTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGGTTAATGAGACGTTTGATAAAc                                                                                                             <  2:275739/141‑1 (MQ=17)

                                  atatTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGc                                                                                                           >  1:330742/1‑141 (MQ=17)

                                  atatTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGc                                                                                                           <  1:38392/141‑1 (MQ=17)

                                    atTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCat                                                                                                         >  2:55858/1‑141 (MQ=12)

                                      ttCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTAATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCatat                                                                                                       <  1:113770/141‑1 (MQ=12)

                                       tCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATg                                                                                                      >  1:289134/1‑141 (MQ=12)

                                        cGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGc                                                                                                     >  2:220834/1‑141 (MQ=12)

                                         gATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCt                                                                                                    >  2:395809/1‑141 (MQ=17)

                                         gATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCt                                                                                                    >  1:325634/1‑141 (MQ=12)

                                         gATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCt                                                                                                    >  1:280942/1‑141 (MQ=11)

                                          aTGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAGCCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATACGCTg                                                                                                   >  2:148591/1‑141 (MQ=17)

                                           tGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGa                                                                                                  >  1:353930/1‑141 (MQ=11)

                                               cAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAGCCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGa                                                                                              >  2:155486/1‑141 (MQ=32)

                                                 aTGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAGCCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACTAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGAtt                                                                                            >  2:230260/1‑141 (MQ=17)

                                                   gACAGTCAGGTTTAAGTTTAAATATAGTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATTGGCTGAATGATTAt                                                                                          >  1:95792/1‑141 (MQ=11)

                                                    gcaatCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTAGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTATAATGAGACGTTTGATACACCCATATGCTGAATGATTAtc                                                                                         <  2:285543/137‑1 (MQ=1)

                                                                                       taaccATTGCTACCTTCGTTGAAGGTTCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTc                                                      <  1:26428/138‑1 (MQ=17)

                                                                                        aGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTcc                                                     >  1:46284/1‑141 (MQ=12)

                                                                                        aGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTcc                                                     <  2:397963/141‑1 (MQ=12)

                                                                                        aGCCATTGCTACCTTCGTTGAAGGTGCTTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTcc                                                     <  1:150148/141‑1 (MQ=17)

                                                                                            aTTGCTACCTTCGTTGCAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCctct                                                 >  1:198023/1‑141 (MQ=17)

                                                                                             ttGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTg                                                >  2:292752/1‑141 (MQ=12)

                                                                                             ttGCTACTTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGCGGTTTACCAAATTGTTTAATGAGACGTTTGACAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTg                                                <  2:40587/141‑1 (MQ=12)

                                                                                             ttGCTACGTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTGCCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCACACAATTATCTAATGAATGTCActctc                                                >  2:46915/1‑140 (MQ=11)

                                                                                              tGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGTCGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTgt                                               >  1:33480/1‑140 (MQ=17)

                                                                                              tGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGc                                               >  1:13815/1‑141 (MQ=32)

                                                                                              tGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGc                                               >  1:372603/1‑141 (MQ=32)

                                                                                              tGCTACCGTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGc                                               <  1:162995/141‑1 (MQ=17)

                                                                                               gctgccTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAACTTGTGTAGTGAGACGTTTGATAAACGCATATGCTGAATGATTTTCTCGATGCTTCCTCAACCCAATATCTAATGTATGTcccgtcgta                                              >  2:143348/5‑135 (MQ=11)

                                                                                                ccaCCTTCGTCGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCACATTGTTTAATGCGACGTTTGATAAACTCAGATGCTGAATTAGTATCTCGTTGCTGACGCAACCAAATATCTAATGTATGTCCCTCTGCAt                                             <  2:94132/139‑1 (MQ=11)

                                                                                                   ccTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCaa                                          <  2:224365/141‑1 (MQ=32)

                                                                                                    cTTCGTTGAAGTTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAATTTGGTTAATGATACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAAt                                         <  2:298450/141‑1 (MQ=11)

                                                                                                     ttCGTTGAAGGTGCCTGATCTTTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATg                                        <  2:194631/141‑1 (MQ=17)

                                                                                                     ttCGTTGAAGGTGCCTGATCTGTAATTACCTTTAAAGCTTTACCGAATTGTTGAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTACTGTATGTCACTCTGCATCCATg                                        <  2:180644/141‑1 (MQ=11)

                                                                                                       tgTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATATAATGTATGTCCCTCTGCATCAATgga                                      >  2:352761/2‑140 (MQ=17)

                                                                                                        gTTGAAGGTGCCTGATCTGTAACCACCTTTTGATGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCa                                     <  1:47687/141‑1 (MQ=11)

                                                                                                            aaGGTGCCTGATCTGTAACTACCTGTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGat                                 <  2:115127/141‑1 (MQ=255)

                                                                                                                  ccTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAAt                           >  1:57507/1‑141 (MQ=12)

                                                                                                                  ccTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAAt                           >  1:311478/1‑141 (MQ=12)

                                                                                                                    tGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTTAATGATTATTTAACTATTTTCTCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAg                         >  1:391640/1‑141 (MQ=11)

                                                                                                                     gagcTGTAATTTCTTTTTGAGGTTTACCAAATTGTTTAATGAGCCGTTTGATAAACGCATATGCGTAATGATTATCTCGTTGTTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGc                        <  1:179389/138‑1 (MQ=11)

                                                                                                                     gATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAGTGGCACGATATAAATAGc                        <  1:318952/141‑1 (MQ=17)

                                                                                                                     gATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGc                        <  1:359652/141‑1 (MQ=32)

                                                                                                                      aTCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCt                       <  2:253442/141‑1 (MQ=17)

                                                                                                                       tCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTc                      <  1:341284/141‑1 (MQ=32)

                                                                                                                       tCTGTAATTACCTGTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTc                      <  1:121836/141‑1 (MQ=17)

                                                                                                                           tAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCACCAtt                  >  2:48715/1‑141 (MQ=17)

                                                                                                                             aTTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGGTCCAtttt                >  2:81214/1‑141 (MQ=17)

                                                                                                                             aTTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGAACGATATAAATAGCACCAttat                >  2:39095/1‑139 (MQ=12)

                                                                                                                              ttATCTTTTGAGGCTTACCTAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTc               <  1:89165/141‑1 (MQ=255)

                                                                                                                              ttACCTTTTGAGGTTTACCTAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTc               <  1:155470/141‑1 (MQ=17)

                                                                                                                               tACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTcc              <  1:259242/141‑1 (MQ=17)

                                                                                                                                aCCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATCTAAATAGCTCCATTTTCCt             >  1:365095/1‑141 (MQ=17)

                                                                                                                                aCCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCt             <  2:202323/141‑1 (MQ=17)

                                                                                                                                 ccTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCtt            <  1:145100/141‑1 (MQ=17)

                                                                                                                                  cTTTTGATGCTTACCAAATTGTTTAATGTGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCttt           <  1:100005/141‑1 (MQ=255)

                                                                                                                                  cTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCttt           <  2:243979/141‑1 (MQ=32)

                                                                                                                                  cTTTTGAGGCTTACCAAATTGTTTAATGAGACGATTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCttt           >  2:263395/1‑141 (MQ=255)

                                                                                                                                  cTTTGGAGGCTTACGAAATTGTGTAATGGGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCttt           <  1:367833/141‑1 (MQ=11)

                                                                                                                                   ttttGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCtttt          <  2:6420/141‑1 (MQ=17)

                                                                                                                                   ttttGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCGCTCTGTATCAATTGCACGATATAAATATGTCCATTTTCCtttt          >  1:84499/1‑141 (MQ=11)

                                                                                                                                   ttttGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGTCACGATATAAATAGCTCCATTTTCCtttt          >  1:269567/1‑141 (MQ=12)

                                                                                                                                   tttcGAGCTTGACCACATTGTTGAATTGGACTTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCtttt          <  2:375596/141‑1 (MQ=11)

                                                                                                                                        aGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCCCCCTTTTCCTTTTAtttt     >  2:60926/1‑141 (MQ=17)

                                                                                                                                         ggTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTACCTCTGCATCAATTGCACGATATAAATAGCTCCATTTTCCTTTTATTTTg    >  1:91188/1‑141 (MQ=17)

                                                                                                                                           tttACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTTCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCttttatttttat  >  1:346321/1‑141 (MQ=17)

TATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAGCCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGAT  >  CP000730/36035‑36314

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`