Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 36,315 G→A 56.0% Y79Y (TAC→TAT USA300HOU_0026 ← IS431mec transposase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP00073036,3150GA56.0% 83.0 / inf 348Y79Y (TAC→TATUSA300HOU_0026IS431mec transposase
Reads supporting (aligned to +/- strand):  ref base G (67/81);  new base A (82/108);  total (156/192)
Fisher's exact test for biased strand distribution p-value = 7.41e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 354 total aligned reads displayed.

TTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTC  >  CP000730/36175‑36455
                                                                                                                                            |                                                                                                                                            
ttACCAAATTGTTTAATGGGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATg                                                                                                                                              <  2:198336/141‑1 (MQ=17)
  aCCAAGTTGTTTAAGGAGCCGTTTGATAAACGTATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTa                                                                                                                                            <  2:371565/141‑1 (MQ=255)
  aCCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATATATGTCGCTCTGCATCCATGGCACGAGATAAATAGCTCACTTTACGTTTTATTTTCAtata                                                                                                                                            >  2:377235/1‑141 (MQ=11)
        ttGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGtctc                                                                                                                                      <  2:130738/141‑1 (MQ=17)
          gTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTTtcat                                                                                                                                    >  1:100732/1‑141 (MQ=12)
          gTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCtcat                                                                                                                                    >  1:170487/1‑141 (MQ=32)
          gTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCtcat                                                                                                                                    <  1:337707/141‑1 (MQ=32)
           tttAATGAGACGTTTGATAAACGCATCTGATGAATGATTATCTCGTTGCTTAAGCAACCAAATATCTAATGTGTGTTCCTCTGCATACATGGCTCGAAAGAAAAAGATCCATTTTCCTATTCATTTGATTTATGtactcaa                                                                                                                                   >  2:40027/1‑135 (MQ=2)
             tAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGTCCCATTTTCCTTTTATTTTGATGTAGGTCACATCaa                                                                                                                                 >  2:76155/1‑141 (MQ=255)
              aaTGAGACCTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAAt                                                                                                                                <  1:367348/141‑1 (MQ=12)
               aggagaCGTGTGAAAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATa                                                                                                                               <  1:3099/139‑1 (MQ=12)
               aTGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATa                                                                                                                               >  2:56117/1‑141 (MQ=32)
                tGAGTCGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGTTATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATAc                                                                                                                              <  1:219894/141‑1 (MQ=17)
                  agccGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCACATGTGCAGTTTTTGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTGATCAATACGc                                                                                                                            <  1:289686/138‑1 (MQ=11)
                   gaCGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGcc                                                                                                                           <  2:305808/141‑1 (MQ=17)
                    aCGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACTTCTCATCAATACGccc                                                                                                                          >  1:42029/1‑140 (MQ=12)
                      gTTTGATAAACGGATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCAtt                                                                                                                        <  2:246926/141‑1 (MQ=12)
                       tttGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATCCGTCTCATCAATACGCCAtct                                                                                                                       <  2:233521/141‑3 (MQ=255)
                        ttGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTTTGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATTTACGTCTCTTAAATACGTCAattt                                                                                                                      >  1:125635/1‑140 (MQ=11)
                        ttGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGTCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATCCGCCATTTg                                                                                                                      >  1:321443/1‑141 (MQ=17)
                           aTAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGtaa                                                                                                                   <  1:158409/141‑1 (MQ=12)
                              aaCGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGtaataa                                                                                                                <  2:200519/141‑1 (MQ=33)
                              aaCGCATATACTGAATGATTATCTAGATGCTTACTCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCTTTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGtaataa                                                                                                                >  1:292971/1‑141 (MQ=11)
                                cGCATATGCTGAATGATTATCTCGTTTCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCACCAATACGCCGTTTGAAATAAGc                                                                                                              >  1:283701/1‑141 (MQ=255)
                                 gtatatGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCt                                                                                                             <  1:333792/139‑1 (MQ=255)
                                  cATATGCCGAATGATTGTCTCGTTGCTTACGCAACCAAAGATCGAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGtaataaccct                                                                                                            >  2:374246/1‑137 (MQ=11)
                                   atatGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGCCCCTCTGCATCAATGGCACGATATACATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCttt                                                                                                           <  1:28020/141‑1 (MQ=25)
                                           aaTGATTATCTCGTTGCTTACGCAACCAAAGATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTACTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCt                                                                                                   <  2:293304/141‑1 (MQ=255)
                                            aTGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTGATAATCgtttttctttat                                                                                                  >  1:152898/1‑135 (MQ=11)
                                               aTTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCttttt                                                                                               >  2:196778/1‑141 (MQ=255)
                                                 tATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTttct                                                                                             <  2:322024/141‑1 (MQ=255)
                                                  ttcccGTTGCTTGCGTAACCAAAGTTCTAATGTATTTCCCTGTGTATCAATGGTACGATATAAATAGCTCCATTTTCCTTGTATTTTGTGATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTttctt                                                                                            <  2:194900/137‑1 (MQ=11)
                                                   tctcGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTttcttc                                                                                           <  2:102466/141‑1 (MQ=255)
                                                   tctcGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTttcttc                                                                                           <  1:163467/141‑1 (MQ=40)
                                                       ggtactcaCGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCCTTTTCTTCCaaa                                                                                       >  2:103414/8‑141 (MQ=255)
                                                        ttGCTCACGCAACCAAATATCTAATGCATGTCCCCCTGTATCAATGGCACGATATAAATAGCACCATTTTCCTTTTATTTTGATACCCGTCTCATCAATACGCCCTTTGTAATCACCTTCTTGATGCGTTTTCTTCCcaac                                                                                      >  2:239559/1‑140 (MQ=11)
                                                            ttACGCAACCAAATACCTAATGTATTTTCCTCTTCATCAACGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGa                                                                                  <  2:1228/141‑1 (MQ=11)
                                                             tACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTATTTCTTCCAAATTTGAt                                                                                 <  1:364654/141‑1 (MQ=255)
                                                              aCGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCTATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAATTTTGATa                                                                                <  1:251053/141‑1 (MQ=255)
                                                               cGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCTTTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATAc                                                                               <  1:23891/141‑1 (MQ=255)
                                                               cGCAACCAAATATCTAATGGATGTCCCGCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATat                                                                               <  1:63778/141‑2 (MQ=255)
                                                                gCAAACAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATata                                                                              <  2:216560/141‑3 (MQ=255)
                                                                ccAACCAAATATCTAATGTATGTCCCTCTGTATCAATGACACGAAATAAATAGCTCCATTTTCCTTTTATTTTGATCTACGTCTCATCAATACGCCGTTGTTAATAACTCTTTTGTTGCTTTTGCTTCATAAttcatgata                                                                              >  2:267480/2‑134 (MQ=11)
                                                                 cAACCAAATATCTAATGTATGTCCCTCTGTATAAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATTCGTCTCATAAATAGGCCATTTTTTATAAGCATTTTTATGCTTTTTGTTCCAAATTTGATaaaa                                                                             >  1:104592/1‑141 (MQ=11)
                                                                 cAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATaa                                                                             >  2:285797/1‑141 (MQ=38)
                                                                    ccAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAAt                                                                          <  1:340049/141‑1 (MQ=37)
                                                                     cAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAAtt                                                                         >  1:206593/1‑141 (MQ=255)
                                                                     cAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAAtt                                                                         >  1:375413/1‑141 (MQ=255)
                                                                      aaaTATCTAATGTATGTCCCTCTGTATTAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTg                                                                        <  2:254597/141‑1 (MQ=255)
                                                                       aagatCTAATGTATGTGCCTCGGTAGCAATGGCACGATATAAAGAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGGTTTTTTTTGCTTTTTCTTCCAGATTTGATATAAAATTgg                                                                       >  2:333987/4‑141 (MQ=11)
                                                                       aaTATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTgg                                                                       <  1:195483/141‑1 (MQ=255)
                                                                            cTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCat                                                                  <  2:139572/141‑1 (MQ=255)
                                                                            cTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCat                                                                  <  1:56117/141‑1 (MQ=37)
                                                                             tAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAACTTGATATAAAATTGGGGCatc                                                                 >  1:387885/1‑140 (MQ=255)
                                                                                tgtatgtCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTTTAATAAGCTTTTTTATGCTTTTTCTTCAAAATTTGATACAAAATTgacacctatac                                                              >  1:186768/1‑131 (MQ=14)
                                                                                 gtatgtCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCt                                                             >  2:227065/1‑141 (MQ=255)
                                                                                 gtatgtCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCt                                                             <  2:316789/141‑1 (MQ=255)
                                                                                    tttCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGa                                                          <  2:272843/139‑1 (MQ=18)
                                                                                      tCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCGTTTTCTTCCAAACGTGATGCACAATTGGGGGATCTTCTTGCAc                                                        >  1:180757/1‑141 (MQ=14)
                                                                                        ccTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAAccc                                                      >  2:301555/1‑141 (MQ=255)
                                                                                           ctGCATAAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCAAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAAc                                                   <  1:69529/141‑1 (MQ=255)
                                                                                             gCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACgg                                                 <  2:106740/141‑1 (MQ=40)
                                                                                               aTCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTa                                               <  2:154053/141‑1 (MQ=21)
                                                                                                tCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATATGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAACTTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAg                                              >  1:377850/1‑141 (MQ=255)
                                                                                                 cAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTCTTTTCTGCTTTTTCTTCCACATTTGATATAAACTTGGGGTATATTCTTGAACCCATCGGTAgt                                             <  2:35830/141‑2 (MQ=11)
                                                                                                 cAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGa                                             <  1:271157/141‑1 (MQ=21)
                                                                                                 aaaTGGTGCGGTATAAATAGTTGCATTTTTCTTTTATTTTGATGTAGGTCTCATGAATGTGCCTTTTGTAGTAAGCTTTTTTATGCTTTTTCTTTCTAATTTGATTTAAAATTGGGGCGTTTTTTTGATCGCAACGGTAgt                                             >  2:134883/2‑140 (MQ=255)
                                                                                                   aTGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGAcc                                           <  1:109971/141‑1 (MQ=39)
                                                                                                    tGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAAAGGTAGATCg                                          >  1:32571/1‑141 (MQ=255)
                                                                                                     ggCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTTTGCTTTTTCTTCCAAATTTGCTATAAAATTAGGGCATATTATTGAACGCCACgagtgatcgt                                         >  1:10772/1‑132 (MQ=11)
                                                                                                        aCGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCACCGGTAGATCGTTGa                                      >  2:108245/1‑141 (MQ=255)
                                                                                                           atatAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAatg                                   >  1:243346/1‑141 (MQ=255)
                                                                                                            tataAATGGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATTAATACGCCATTTGTAATAAGCTTTTTTATGTTTTTTCTTCCTAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAatga                                  <  2:51290/141‑1 (MQ=11)
                                                                                                            tataAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAatga                                  >  2:149092/1‑141 (MQ=21)
                                                                                                            tataAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATCAAATTGGGGCATATTCTTGAACCCAATGGTAGATCGTTGAatga                                  >  2:4583/1‑141 (MQ=11)
                                                                                                            tataAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAatga                                  >  2:390374/1‑141 (MQ=255)
                                                                                                             ataAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAatgat                                 <  2:349946/141‑1 (MQ=21)
                                                                                                              taAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCAGATTCTTCAAACCACCGGCAGATCTGTGAataatg                                >  1:50953/1‑141 (MQ=11)
                                                                                                                    gCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGGAGACCGTTGAATGATGAACGtt                          >  1:82115/1‑141 (MQ=21)
                                                                                                                    gCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAACATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGtt                          <  1:264108/141‑1 (MQ=255)
                                                                                                                     cTCCATTTTCCTTTTATCTTGATCTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGTGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGttt                         <  2:172714/141‑1 (MQ=11)
                                                                                                                      tCCATTTTTCTTTTATTTTGATATACGTCTCAACAATACGCCATTTGTTATAAGCTTTTTTATGCTTTTTTTTCCAAATTTGATATAAAATTTTTGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTa                        <  2:188482/141‑1 (MQ=11)
                                                                                                                         aTTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTacac                     <  1:228028/141‑1 (MQ=40)
                                                                                                                          ttttCCTTTTATTTTGATATCCGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTTTGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTAcacc                    <  2:311478/141‑1 (MQ=11)
                                                                                                                          ttttCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGGTTGTTGAATGATGAACGTTTAcacc                    <  2:384584/141‑1 (MQ=11)
                                                                                                                           tttCTTTTTATTTTGATATACGTCTGATCAATACGCCATTTGTAATAAGCTTTTTTCTGCTTTTTCTTCCAAATTTGATATAGAATTGGGGCATAGTCTTGACCCCAACGGTAGATCGTTGAATGATGAACGTTTAcacca                   <  2:178242/141‑1 (MQ=11)
                                                                                                                           tttCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTAcacca                   <  1:224928/141‑1 (MQ=18)
                                                                                                                           tttCCTTTTATTTTGATATACGGCTCATCAATACGCCGTTTGTAATAAGCTTTTTTATGCTTTTTCTTCCCAATTTGATATAAAATTGGCGCATGTTCTTGAACCGAACGGTGAATCGTTGAATGGTGAACGTTTAcaccc                   >  2:65178/1‑140 (MQ=11)
                                                                                                                             tCCTTTTATTTCGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACg                 <  1:113942/141‑1 (MQ=21)
                                                                                                                              ccTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGGATGATGAACGTTTACACCACGt                >  1:118701/1‑141 (MQ=255)
                                                                                                                               cTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGtt               >  1:346943/1‑141 (MQ=25)
                                                                                                                               cTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGtt               >  1:137503/1‑141 (MQ=255)
                                                                                                                                tgttATTTTGATATACGTTTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTACCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTc              <  2:84499/139‑1 (MQ=11)
                                                                                                                                   tATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTCTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCt           <  1:303759/141‑1 (MQ=21)
                                                                                                                                    aTTTTGATGTACGTGTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCtt          <  2:169728/141‑1 (MQ=21)
                                                                                                                                    aTTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCtt          <  2:338021/141‑1 (MQ=11)
                                                                                                                                     ttttGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTa         <  2:162852/141‑1 (MQ=21)
                                                                                                                                      tttGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACTACTGAATGATGAACGTTTACACCACGTTCCCTTaa        <  1:192682/141‑1 (MQ=255)
                                                                                                                                            gTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGCATGATGAACGTTTCCACCCCGTTCCCTTAATCtttt  >  1:356129/1‑140 (MQ=255)
                                                                                                                                            gTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTc  >  1:218434/1‑141 (MQ=40)
                                                                                                                                            |                                                                                                                                            
TTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTC  >  CP000730/36175‑36455

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: