BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP000730	36,378	C→T	88.2%	L58L (TTG→TTA)	USA300HOU_0026 ←	IS431mec transposase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	36,378	0	C	T	88.2%	494.0 / 57.5	268	L58L (TTG→TTA)	USA300HOU_0026	IS431mec transposase
Reads supporting (aligned to +/- strand): ref base C (17/15); new base T (114/120); total (133/135)
Fisher's exact test for biased strand distribution p-value = 7.08e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 270 total aligned reads displayed.

CGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAAC  >  CP000730/36238‑36515

 tcAACCAAATTTCTAATGTAGCTCCGTCTGCATAAATGGCACCATATAAATATCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCACTTGTAATAAGCTTTTTTATGCTTGTCCTTCCAAATTTGATACa                                                                                                                                          <  2:148614/140‑1 (MQ=14)

  cAACCAAATATCTAATGTATGTCCCTCTGTATAAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATTCGTCTCATAAATAGGCCATTTTTTATAAGCATTTTTATGCTTTTTGTTCCAAATTTGATaaaa                                                                                                                                         >  1:104592/1‑141 (MQ=11)

     ccAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAAt                                                                                                                                      <  1:358839/141‑1 (MQ=37)

     ccAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAAt                                                                                                                                      >  2:313882/1‑141 (MQ=38)

      cAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAAtt                                                                                                                                     >  1:206593/1‑141 (MQ=255)

       aaaTATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTg                                                                                                                                    <  1:102167/141‑1 (MQ=255)

        aaTATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTgg                                                                                                                                   <  1:195483/141‑1 (MQ=255)

          tatCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTgggg                                                                                                                                 <  1:81214/141‑1 (MQ=255)

          tatCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTgggg                                                                                                                                 <  2:15706/141‑1 (MQ=37)

                atgtatgtCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCCTACAAATTTGCTATAAATTTTGGGCATAtt                                                                                                                           >  1:324813/1‑141 (MQ=14)

                 tgtatgtCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTTTAATAAGCTTTTTTATGCTTTTTCTTCAAAATTTGATACAAAATTgacacctatac                                                                                                                          >  1:186768/1‑131 (MQ=14)

                     tgtCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGAATATTCTTGa                                                                                                                      >  2:383382/1‑141 (MQ=11)

                     tgtCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGa                                                                                                                      >  1:286197/1‑141 (MQ=37)

                       tCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCGTTTTCTTCCAAACGTGATGCACAATTGGGGGATCTTCTTGCAc                                                                                                                    >  1:180757/1‑141 (MQ=14)

                        cccTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAAcc                                                                                                                   >  2:340498/1‑141 (MQ=255)

                         ccTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAAccc                                                                                                                  >  2:301555/1‑141 (MQ=255)

                           tctGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATTTACGTCTCATCTATACGCCCTTTTTAATCAGCTTTTTTATGCTTTTTCTTCCTATTTTGATATAAACTTGGGTCTTATTGTTGTAccctc                                                                                                                >  1:127429/1‑139 (MQ=11)

                            ctGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATTCTTTTTCTTCCAAATACAATAAAAAATTGGGGCACCATACTGAACCCAAc                                                                                                               >  2:137219/1‑141 (MQ=11)

                            cgtaaTCAATGGCACGATATAAATAGCTTCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCatc                                                                                                               <  2:46972/137‑3 (MQ=255)

                              gtaTCAATGGCACGATATAAATAGCTCCATTTTCCTGTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACgg                                                                                                             <  2:76482/139‑1 (MQ=255)

                               taTCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGTTATAAAATTGGGGCATATTCTTGAACCCAACggg                                                                                                            >  2:375320/2‑140 (MQ=255)

                               taTCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGt                                                                                                            >  2:303746/2‑141 (MQ=255)

                               taTCAATGGCACGATATAAATAGCTCCATGTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGt                                                                                                            >  2:192646/2‑141 (MQ=255)

                               cATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGt                                                                                                            <  1:17110/141‑1 (MQ=18)

                                aTCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTa                                                                                                           <  2:373149/141‑1 (MQ=21)

                                  cAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTCTTTTCTGCTTTTTCTTCCACATTTGATATAAACTTGGGGTATATTCTTGAACCCATCGGTAgt                                                                                                         <  2:35830/141‑2 (MQ=11)

                                  cAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGa                                                                                                         <  1:271157/141‑1 (MQ=21)

                                    aTGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAATCTTTTTTATGCTTATTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGAcc                                                                                                       <  1:374170/141‑1 (MQ=255)

                                    aTGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGAcc                                                                                                       <  1:109971/141‑1 (MQ=39)

                                     tGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCg                                                                                                      >  1:182176/1‑141 (MQ=255)

                                     tGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAAAGGTAGATCg                                                                                                      >  1:32571/1‑141 (MQ=255)

                                         aCGATATAAATCGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGa                                                                                                  <  1:46915/141‑1 (MQ=18)

                                         aCGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCACCGGTAGATCGTTGa                                                                                                  >  2:108245/1‑141 (MQ=255)

                                         aCGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGa                                                                                                  >  2:264670/1‑141 (MQ=255)

                                            atatAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAatg                                                                                               <  1:384140/141‑1 (MQ=18)

                                            atatAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAatg                                                                                               >  1:243346/1‑141 (MQ=255)

                                             tataAATGGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATTAATACGCCATTTGTAATAAGCTTTTTTATGTTTTTTCTTCCTAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAatga                                                                                              <  2:51290/141‑1 (MQ=11)

                                             tataAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATCAAATTGGGGCATATTCTTGAACCCAATGGTAGATCGTTGAatga                                                                                              >  2:4583/1‑141 (MQ=11)

                                               taAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATCAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAatgatg                                                                                            >  2:230086/1‑141 (MQ=21)

                                               taAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCAGATTCTTCAAACCACCGGCAGATCTGTGAataatg                                                                                            >  1:50953/1‑141 (MQ=11)

                                                aaaTAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAAtgatga                                                                                           >  1:219709/1‑141 (MQ=18)

                                                 aaTAGCTCCATTTTCCTTTTATTTGGATAGACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGaa                                                                                          >  1:193407/1‑141 (MQ=255)

                                                      cTCCATTTTCCTTTTATCTTGATCTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGTGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGttt                                                                                     <  2:172714/141‑1 (MQ=11)

                                                       tCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTa                                                                                    <  1:340498/141‑1 (MQ=255)

                                                         cATTTTCCTTTTATTTTGTTCTACGTCGCATCAATAAGTCATTTTTAGTAAGCTTTTTTGTGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTaca                                                                                  <  2:238523/141‑1 (MQ=11)

                                                         cATTTTCCGTTTATTGTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGGTATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTaca                                                                                  <  2:129818/141‑1 (MQ=11)

                                                          aTTTTTCTTTTTTTTTGATATACTTCTCATCAATACGGCATTTGTATTAAGGTATTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGTCATATTCTTGAACCCAACGGTAGATGGTTGAATGATGTACGTTTacac                                                                                 <  2:162683/141‑1 (MQ=11)

                                                          aTTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTacac                                                                                 <  1:228028/141‑1 (MQ=40)

                                                          aTTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTacac                                                                                 <  1:337895/141‑1 (MQ=255)

                                                           ttttCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTAcacc                                                                                >  1:142476/1‑141 (MQ=25)

                                                           tttgCCTTTTATTTTGATGTACGTCTCATCAATACGCCATATGTTATAAGCAATTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATACTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTAcacc                                                                                <  2:280822/141‑1 (MQ=11)

                                                            tttCTTTTTATTTTGATATACGTCTGATCAATACGCCATTTGTAATAAGCTTTTTTCTGCTTTTTCTTCCAAATTTGATATAGAATTGGGGCATAGTCTTGACCCCAACGGTAGATCGTTGAATGATGAACGTTTAcacca                                                                               <  2:178242/141‑1 (MQ=11)

                                                            tttCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATACGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTAcacca                                                                               <  1:30810/141‑1 (MQ=11)

                                                            tttCCTTTTATTTTGATATACGGCTCATCAATACGCCGTTTGTAATAAGCTTTTTTATGCTTTTTCTTCCCAATTTGATATAAAATTGGCGCATGTTCTTGAACCGAACGGTGAATCGTTGAATGGTGAACGTTTAcaccc                                                                               >  2:65178/1‑140 (MQ=11)

                                                              tCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACg                                                                             <  2:330742/141‑1 (MQ=18)

                                                               ccTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGt                                                                            >  2:202139/1‑141 (MQ=21)

                                                               ccTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGGATGATGAACGTTTACACCACGt                                                                            >  1:118701/1‑141 (MQ=255)

                                                               ccTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGt                                                                            >  1:277811/1‑141 (MQ=255)

                                                                   ttATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGACCCCAACGGTAGACCGTTGAATGCTGAACGTTTACACCACGTTccc                                                                        <  2:292971/141‑1 (MQ=255)

                                                                    tgttttTATATACGTCTGATCAATACGCCATTTGTAATAAGTTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGTGCATATTCTTGAACCCAACGGTAGATCGGGGAATTATGAAAGTTTACACCACGTTctct                                                                       <  2:102962/139‑4 (MQ=11)

                                                                    tagtttgagacaCGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCTTTTAATGATGAACGTTTACACCACGTTctct                                                                       <  1:56558/130‑4 (MQ=11)

                                                                    tATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCt                                                                       <  1:58679/141‑1 (MQ=18)

                                                                     tttttGATGTTCGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCtt                                                                      <  2:35368/140‑1 (MQ=21)

                                                                     tttttGATGTACGTCTCATCAATACGCCTTTTGTAATAAGCTTTTTTATGCCATTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCtt                                                                      <  1:288493/140‑1 (MQ=255)

                                                                     aTTTTGATGTACGTGTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCtt                                                                      <  2:169728/141‑1 (MQ=21)

                                                                      ttttGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTa                                                                     <  2:391640/141‑1 (MQ=21)

                                                                         ttatatACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAata                                                                  <  1:351777/136‑1 (MQ=11)

                                                                         tGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAata                                                                  <  2:33480/141‑1 (MQ=11)

                                                                          gatatACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCCCGCTCTCTGAatat                                                                 >  2:213647/5‑141 (MQ=11)

                                                                          gATATACGTCTCATCTATACGCCATTCGTAATAATCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATGCTTGAACCCAACGTTAGATCGTTGAATGATGAACGTTGACACCACGTTCTCTTAatat                                                                 <  1:241795/141‑1 (MQ=11)

                                                                            tGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATAttt                                                               >  2:320881/1‑141 (MQ=21)

                                                                             gTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGCATGATGAACGTTTCCACCCCGTTCCCTTAATCtttt                                                              >  1:356129/1‑140 (MQ=255)

                                                                              tACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTGCCGTTAATATTTCa                                                             >  1:259653/1‑141 (MQ=255)

                                                                              tACGTCTCATCAATAAGACATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTTAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCa                                                             <  1:94645/141‑1 (MQ=11)

                                                                               aCGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAg                                                            <  1:86283/141‑1 (MQ=255)

                                                                               aCGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAg                                                            <  1:163831/141‑1 (MQ=21)

                                                                                cGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGa                                                           >  1:223568/1‑141 (MQ=255)

                                                                                 gTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGat                                                          <  2:225958/141‑1 (MQ=255)

                                                                                  tctcATCAATACGCCATTTATAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATAGAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGata                                                         >  1:239291/1‑141 (MQ=255)

                                                                                       tcaATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCa                                                    <  2:199677/141‑1 (MQ=18)

                                                                                          aTACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGa                                                 <  2:31153/141‑1 (MQ=39)

                                                                                           tACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGAt                                                >  1:134355/1‑141 (MQ=40)

                                                                                           tACGCCATTTGTAATAAGCTTTTTTATGCTTCTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTGATATTTCAGATATATAACGAt                                                >  2:165538/1‑141 (MQ=11)

                                                                                             cGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTGCCCTTAATATTTCAGATATATCACGATaa                                              >  1:301220/1‑141 (MQ=18)

                                                                                               ccATTTGTAATAAGCTTTTTTATGGTTTTTCTTCCAAATTTGATATAAAATTGTTGCATATTCTTGAACCCCACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTATTAATATTTCCACTGTATCTCGATAGCt                                            >  2:134818/1‑141 (MQ=11)

                                                                                               ccATTTGTAATAAGCTTTTTTATGCTTTTTGTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGTTGAAAGTATACTCCTCGTTCTCTTAATATTTCAGATATATCACGATaggt                                            >  2:82804/1‑138 (MQ=11)

                                                                                               ccATTTGTAATAAGCTTTTTTATGCTTTTTCTTTCAAATTTGATACAAAATTGGGGCATATTTTTGAACCCCACGGTAGACCGTTGCATGCTGAACGTTTACACCACGTTCCCTTAATGTTTCAGATGTATAACGAttcct                                            >  2:152181/1‑137 (MQ=14)

                                                                                                cATTTGTAATAAGCTTTTTTATGCTTTTTCTTTCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGACTGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTc                                           <  1:266514/141‑1 (MQ=255)

                                                                                                cATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTc                                           <  1:334411/141‑1 (MQ=18)

                                                                                                cATTTGTAATAAGCTTTTGTGATCTTTTTCTTCCACATTTTATATAAATTTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACTTTCACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTc                                           <  2:92562/141‑1 (MQ=11)

                                                                                                   ttGTAATAAGCTTGTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAACGATGAACGTTTACACCACGTTCTCTTAATATTTCAGCTATATCACGATAGCTCAAt                                        <  2:1112/141‑1 (MQ=11)

                                                                                                    tGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATAGCTCAAta                                       >  1:148163/1‑140 (MQ=11)

                                                                                                     gTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTATTGGCATATCACGATAACTCAATgc                                      <  1:395809/141‑2 (MQ=11)

                                                                                                      taataaGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATAGCTCAATgca                                     <  2:186398/141‑3 (MQ=11)

                                                                                                      taataaGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATAGCTCAATgca                                     >  2:286614/1‑139 (MQ=11)

                                                                                                      taataaGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATAGCTCAATgca                                     <  1:68824/141‑3 (MQ=11)

                                                                                                      taataaGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGta                                     >  2:38757/1‑141 (MQ=40)

                                                                                                       aataaGCTTTTTTATTTTTTTTCTTCCAAATATGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATgcaa                                    >  2:250805/1‑138 (MQ=11)

                                                                                                       aataaGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATAGCTCAATgcat                                    <  2:120660/141‑4 (MQ=11)

                                                                                                                ttttATGCTTTTTCTTGCATGTTTGATACAAAATTGGGGCAAAGTCTTGAACCCAACGGTAGAGCGTTGAATGATGAAGGTTTACACCACGTCCCCGTAATATGTCAAGTATATCACGATACTTCaagtttcagcttatat                           >  2:264159/1‑127 (MQ=255)

CGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAAC  >  CP000730/36238‑36515

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`