BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP000730	36,444	C→T	43.4%	R36R (AGG→AGA)	USA300HOU_0026 ←	IS431mec transposase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	36,444	0	C	T	43.4%	70.6 / 122.6	126	R36R (AGG→AGA)	USA300HOU_0026	IS431mec transposase
Reads supporting (aligned to +/- strand): ref base C (27/44); new base T (32/23); total (59/67)
Fisher's exact test for biased strand distribution p-value = 3.11e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.95e-01

Only 100 of 128 total aligned reads displayed.

TTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTAT  >  CP000730/36305‑36579

ttATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGACCCCAACGGTAGACCGTTGAATGCTGAACGTTTACACCACGTTccc                                                                                                                                        <  2:292971/141‑1 (MQ=255)

tgaTTTTGATGTACGTCTCATCAATACTCCATTTGTAATAAGCTTTTTTATGCTTTTTCGTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTccc                                                                                                                                        <  2:63748/139‑1 (MQ=255)

 tATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCt                                                                                                                                       <  1:58679/141‑1 (MQ=18)

 tATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTCTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCt                                                                                                                                       <  1:303759/141‑1 (MQ=21)

  tttttGATGTTCGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCtt                                                                                                                                      <  2:35368/140‑1 (MQ=21)

  tttttGATGTACGTCTCATCAATACGCCTTTTGTAATAAGCTTTTTTATGCCATTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCtt                                                                                                                                      <  1:288493/140‑1 (MQ=255)

  tttttGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCtt                                                                                                                                      <  1:55858/140‑1 (MQ=21)

  tttttGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCtt                                                                                                                                      <  2:22972/140‑1 (MQ=18)

  tttttGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCTCGTTCTCtt                                                                                                                                      <  1:292752/140‑1 (MQ=11)

  aTTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCtt                                                                                                                                      <  2:338021/141‑1 (MQ=11)

  aTTGTGATGAACGCCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTGCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGAAGCCCGTTGAATGATGAATGTTTACACCACGTTCCCtt                                                                                                                                      <  1:114021/141‑1 (MQ=14)

   ttttGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTa                                                                                                                                     <  2:162852/141‑1 (MQ=21)

   ttttGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTa                                                                                                                                     <  2:286197/141‑1 (MQ=18)

   ttttGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTa                                                                                                                                     <  1:314251/141‑1 (MQ=255)

    tttGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACTACTGAATGATGAACGTTTACACCACGTTCCCTTaa                                                                                                                                    <  1:192682/141‑1 (MQ=255)

    tttGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTaa                                                                                                                                    >  1:214834/1‑141 (MQ=25)

    tttGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTaa                                                                                                                                    <  2:388099/141‑1 (MQ=11)

      ttatatACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAata                                                                                                                                  <  1:351777/136‑1 (MQ=11)

       gatatACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCCCGCTCTCTGAatat                                                                                                                                 >  2:213647/5‑141 (MQ=11)

       gATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGCTATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAGTGGTGAACGTTTAAACCACGTCCCCTTActat                                                                                                                                 >  2:80190/1‑141 (MQ=11)

       gATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAatat                                                                                                                                 >  1:69425/1‑141 (MQ=21)

       gATATACGTCTCATCTATACGCCATTCGTAATAATCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATGCTTGAACCCAACGTTAGATCGTTGAATGATGAACGTTGACACCACGTTCTCTTAatat                                                                                                                                 <  1:241795/141‑1 (MQ=11)

         tGTACGTCTCATCAATGCGCCATTTGTAATATTTTTTTGTTTGATCGTTATCCGAAATTTGATATAAAATTGCGGCATAGTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATAttt                                                                                                                               <  1:188047/141‑1 (MQ=11)

         tGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATAttt                                                                                                                               >  2:320881/1‑141 (MQ=21)

          gTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGCATGATGAACGTTTCCACCCCGTTCCCTTAATCtttt                                                                                                                              >  1:356129/1‑140 (MQ=255)

           tACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTGCCGTTAATATTTCa                                                                                                                             >  1:259653/1‑141 (MQ=255)

           tACGTCTCATCAATAAGACATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTTAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCa                                                                                                                             <  1:94645/141‑1 (MQ=11)

            aCGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAg                                                                                                                            <  1:86283/141‑1 (MQ=255)

            aCGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAg                                                                                                                            <  1:163831/141‑1 (MQ=21)

             cGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTCATCTTTCAGa                                                                                                                           >  2:378845/1‑141 (MQ=11)

             cGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGa                                                                                                                           >  1:223568/1‑141 (MQ=255)

              gTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGat                                                                                                                          <  2:225958/141‑1 (MQ=255)

               tctcATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCCCGTTCTCTTAATATTTCAGata                                                                                                                         >  2:298470/1‑141 (MQ=255)

               tctcATCAATACGCCATTTATAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATAGAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGata                                                                                                                         >  1:239291/1‑141 (MQ=255)

                ctcATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGatat                                                                                                                        <  2:46284/141‑1 (MQ=255)

                  catcaATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCCCGTTCTCTTAATATTTCAGatatat                                                                                                                      >  1:35818/1‑141 (MQ=255)

                  catcaATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGatatat                                                                                                                      <  2:152898/141‑1 (MQ=255)

                  catcaATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGatatat                                                                                                                      >  1:336597/1‑141 (MQ=255)

                   atcaATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCCAGTTCTCTTAATATTTCAGATATATc                                                                                                                     >  2:392100/1‑141 (MQ=11)

                     caATACGCCATTTGTAATAAGCTTTTTTTTTTTTTTTTTTCCAAATTAAAAATAAAATTTGGGCACATACTTGAACCCAACGGTAGAGCGTTGACATACGAACGTTTACACCACCTACTATTAATCTTTCAGAAATATcct                                                                                                                   >  2:27199/1‑139 (MQ=255)

                     caATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCCCGATCTCTTAATATTTCATATATATCAc                                                                                                                   >  2:289858/1‑141 (MQ=11)

                     caATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCag                                                                                                                   >  2:353377/1‑140 (MQ=255)

                     caATACGCCATTTGTAATAAACTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATCGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACATTCTCTTAATATTTCAGATATATCAc                                                                                                                   >  1:243280/1‑141 (MQ=11)

                      aaTACGCTATTTGTAATAAGATTTTTTATGCTTTTTCTTCCAAATTTGTTACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACg                                                                                                                  <  1:172803/141‑1 (MQ=255)

                      aaTACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATCTTTCTAATATATCAca                                                                                                                  >  2:210753/1‑140 (MQ=11)

                       aTACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGa                                                                                                                 <  2:168316/141‑1 (MQ=255)

                       aTACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGa                                                                                                                 >  1:128370/1‑141 (MQ=255)

                       aTACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGa                                                                                                                 >  1:134278/1‑141 (MQ=21)

                       aTACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGa                                                                                                                 <  2:31153/141‑1 (MQ=39)

                        tACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGAt                                                                                                                <  1:148591/141‑1 (MQ=40)

                        tACGCCATTTGTAATAAGCTTTTTTATGCTTCTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTGATATTTCAGATATATAACGAt                                                                                                                >  2:165538/1‑141 (MQ=11)

                        tACGCCATTGGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATCTTCTTGAACCCAACGGTAGATCGTTGAATGATGACCGTTTACACAACGTTCTCTTCATGTTTCGGGTCTATCActag                                                                                                                >  2:312135/1‑138 (MQ=11)

                         aCGCCATTTGTAATAAGCTTTTTTTTTCGTTGTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATa                                                                                                               >  2:85716/1‑141 (MQ=11)

                          cGCCATTTGTAATAAGATTTTGTCTGCTTTTTCTTCCAAATTTTATATCAAATTGGGGCATATTCTTGAACCCAACTGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATaa                                                                                                              <  1:33245/141‑1 (MQ=11)

                            ccATTTGTAATAAGCTTTTTTATGGTTTTTCTTCCAAATTTGATATAAAATTGTTGCATATTCTTGAACCCCACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTATTAATATTTCCACTGTATCTCGATAGCt                                                                                                            >  2:134818/1‑141 (MQ=11)

                            ccATTTGTAATAAGCTTTTTTATGCTTTTTCTTTCAAATTTGATACAAAATTGGGGCATATTTTTGAACCCCACGGTAGACCGTTGCATGCTGAACGTTTACACCACGTTCCCTTAATGTTTCAGATGTATAACGAttcct                                                                                                            >  2:152181/1‑137 (MQ=14)

                             cATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATAGCTc                                                                                                           <  2:223568/141‑1 (MQ=11)

                             cATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATAGCTc                                                                                                           >  2:48766/1‑141 (MQ=255)

                             cATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTc                                                                                                           <  1:334411/141‑1 (MQ=18)

                             cATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGCTGAATGAGGAACTCTTGAACCAGGTTCCCTTAATATTTCAGATATATCACGATAACTc                                                                                                           <  1:94094/141‑1 (MQ=11)

                             cATTTGTAATAAGCTTTTGTGATCTTTTTCTTCCACATTTTATATAAATTTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACTTTCACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTc                                                                                                           <  2:92562/141‑1 (MQ=11)

                              aTTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATAGCTCa                                                                                                          >  1:276243/1‑141 (MQ=255)

                               tttGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATAGCTCaa                                                                                                         <  2:170851/141‑1 (MQ=11)

                                ttGTAATAAGCTTTTTTATGCTTTTTTTTTTTTTTTTTATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATTTTTTATATATATCACTAtggcttaat                                                                                                        >  2:325071/1‑133 (MQ=11)

                                ttGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAATGAATTCACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAAt                                                                                                        >  1:30691/1‑141 (MQ=11)

                                ttGTAATAAGCTTGTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAACGATGAACGTTTACACCACGTTCTCTTAATATTTCAGCTATATCACGATAGCTCAAt                                                                                                        <  2:1112/141‑1 (MQ=11)

                                 tGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATAGCTCAAta                                                                                                       >  1:148163/1‑140 (MQ=11)

                                 tGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATAGCTCAATg                                                                                                       <  1:196778/141‑1 (MQ=11)

                                 tGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATg                                                                                                       <  1:177782/141‑1 (MQ=18)

                                  gTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTATTGGCATATCACGATAACTCAATgc                                                                                                      <  1:395809/141‑2 (MQ=11)

                                   taataaGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATAGCTCAATgca                                                                                                     <  1:143348/141‑3 (MQ=11)

                                   taataaGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATAGCTCAATgca                                                                                                     <  2:186398/141‑3 (MQ=11)

                                   taataaGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGCCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGCACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATgca                                                                                                     <  1:99486/141‑3 (MQ=255)

                                   taataaGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGta                                                                                                     >  2:38757/1‑141 (MQ=40)

                                    aataaGCTTTTTTATTTTTTTTCTTCCAAATATGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATgcaa                                                                                                    >  2:250805/1‑138 (MQ=11)

                                    aataaGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATAGCTCAATgcat                                                                                                    <  2:120660/141‑4 (MQ=11)

                                    aataaGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAACGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATgcat                                                                                                    <  2:74768/141‑4 (MQ=9)

                                    aataaGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGtat                                                                                                    >  2:264235/1‑141 (MQ=255)

                                             ttttATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGAt                                                                                           <  1:38757/141‑1 (MQ=35)

                                                tATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGAtagt                                                                                        >  2:299495/1‑141 (MQ=35)

                                                   gCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATAACTTCGAtagtatc                                                                                     >  2:1162/1‑139 (MQ=255)

                                                        ttcttcCAAATTTGATACAAAATTGGGTCCTATTCTTGAACCCAACGTTATATCGTTGAATGATCAACTTTTACACCACGTTCCCTTAATATTTCAGATATATCACTATAACTCAATCTATATCTTAGATAGTAGCCAACg                                                                                <  2:111098/141‑1 (MQ=11)

                                                                              ttGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGt                                                          <  1:39065/141‑1 (MQ=32)

                                                                              ttGGAGCATATTCTTGAACCTAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTGCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGt                                                          <  1:39095/141‑1 (MQ=12)

                                                                                   gCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAAt                                                     <  1:264235/141‑1 (MQ=32)

                                                                                            cgAACCCAACGGTTGACCGTGGAATGAGGAACGTTTACACCACGATGCCATAATATTTCAGATATATCATAATAACTCAATGTATATCTTAGATAGTAGACAACGGCTACGGTGATAACATCCTTGTTAAAATGTTtataa                                            >  2:189242/2‑140 (MQ=11)

                                                                                                cccAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGa                                        >  1:84376/1‑141 (MQ=32)

                                                                                                    aCGGTAGATCGTTGAATGATGAACGTTTACACCACGGTCTCTTAATATTTCAGATATATCACGATAACTCCATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATCACGCCTTTATTATATTTTTTATAACTGACATa                                    >  2:60503/1‑141 (MQ=11)

                                                                                                     cgttAGACCTTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAg                                   <  2:356129/138‑1 (MQ=17)

                                                                                                     cGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAg                                   <  2:158661/141‑1 (MQ=32)

                                                                                                     cGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAg                                   <  2:218434/141‑1 (MQ=32)

                                                                                                       gTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGtt                                 <  1:230260/141‑1 (MQ=32)

                                                                                                         aGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCa                               <  1:332490/141‑1 (MQ=32)

                                                                                                         aGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCa                               <  1:76155/141‑1 (MQ=32)

                                                                                                                  aaTGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACagaaga                      <  1:1162/141‑1 (MQ=32)

                                                                                                                   atgatgAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGAc                     >  1:142964/1‑141 (MQ=32)

                                                                                                                     gatgaACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTc                   <  1:43733/141‑1 (MQ=32)

                                                                                                                       tgaACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTACACTGTTCATATCTGAAATAGTTCCTACAGAAGactact                 >  2:93103/1‑141 (MQ=11)

                                                                                                                              ttACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGtt          <  1:305376/141‑1 (MQ=32)

                                                                                                                                      cGTTCCCTTAATATTTCAGATATATCACGATCACTCAATGTATATCTTAGCTAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAGATtat  <  2:180757/141‑1 (MQ=12)

TTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTAT  >  CP000730/36305‑36579

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`