Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 76,322 G→A 52.1% intergenic (+380/‑754) USA300HOU_0072 → / → USA300HOU_0075 universal stress protein/lysophospholipase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP00073076,3220GA52.1% 1.1 / 315.7 246intergenic (+380/‑754)USA300HOU_0072/USA300HOU_0075universal stress protein/lysophospholipase
Reads supporting (aligned to +/- strand):  ref base G (60/57);  new base A (55/71);  total (117/129)
Fisher's exact test for biased strand distribution p-value = 2.49e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 253 total aligned reads displayed.

ATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCATAAAAAGCTAATTACAAATGGCGTATTGATGAGACGTACATCAAAA  >  CP000730/76182‑76457
                                                                                                                                            |                                                                                                                                        
 taGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAgga                                                                                                                                         >  2:82115/1‑140 (MQ=17)
 taGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAggg                                                                                                                                         <  2:367886/141‑1 (MQ=32)
  aatataATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAGATATTAAgaga                                                                                                                                        <  2:36165/139‑1 (MQ=12)
       aaTTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACgtg                                                                                                                                   >  1:306025/1‑141 (MQ=17)
          tttAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATTTAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACgtggtg                                                                                                                                <  2:51577/141‑1 (MQ=12)
          tttAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATGTCTGATATATTAAGAGAACgtggtg                                                                                                                                <  1:218532/141‑1 (MQ=255)
           ttAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGCGAACGTGgtgt                                                                                                                               >  1:333987/1‑141 (MQ=17)
            tAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTa                                                                                                                              <  1:141357/141‑1 (MQ=12)
             aaCAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATCTTAAGGGAACGTGGTGTaa                                                                                                                             >  2:169282/1‑141 (MQ=17)
              aCAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTaaa                                                                                                                            >  1:101154/1‑141 (MQ=17)
              aCAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTaaa                                                                                                                            >  1:9769/1‑141 (MQ=17)
                aaaaaGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACg                                                                                                                          <  1:176040/141‑1 (MQ=17)
                   actgatccttctGTATGAACTATTTTAGATATATACATTTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTTTCTAAGATATGCATTGTGTTATCTTGATATATCTGAAACATTAAGGGAACGTAGTGTAAACGTtc                                                                                                                       <  2:390550/134‑1 (MQ=11)
                   aaGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGtta                                                                                                                       >  2:353752/1‑140 (MQ=17)
                    aGGAGTCCTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTtca                                                                                                                      <  1:359371/141‑1 (MQ=12)
                     ggAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTtcat                                                                                                                     >  1:17420/1‑141 (MQ=32)
                      gAGTCTTCTGTATGGACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTtcatc                                                                                                                    <  1:88709/141‑1 (MQ=12)
                       ggTCTTCTGTATGAACGATTTCAGCTATAATCAATTTAAAAGGGGTGTTTTGACGGTCGTAGTTGTCCACTATCTACTGTATGCATTGAGTTGTCGCTATATATCTGAAATATTAAGGGAACGTGGTGTTAACGTtcatca                                                                                                                   <  2:156978/140‑1 (MQ=2)
                       aGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTtcatca                                                                                                                   <  1:69791/141‑1 (MQ=12)
                        gTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATGGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTcatcat                                                                                                                  >  1:134883/1‑141 (MQ=12)
                        ctcttttGTATTAACTATTTCATATATAAACCATTTAACAGGGATTTTATATCTTTAGCGGTTGGCTACCATCTAAGATATGCATTGAGCTCTCGTTATATATCTGAATTATTAAGAGAACTTGGTGTAAACGTTcatcat                                                                                                                  <  2:65450/140‑1 (MQ=11)
                            tctGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCaa                                                                                                              <  2:178168/141‑1 (MQ=17)
                              tGTATGAACTATTTGAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAAAGAACGTGGTGTAAACGTTCATCATTCAACg                                                                                                            >  2:270988/1‑141 (MQ=255)
                              tGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACg                                                                                                            >  1:276777/1‑141 (MQ=17)
                              tGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAAAGTGGTGTAAACGTTCATCATTCAACg                                                                                                            <  2:247857/141‑1 (MQ=12)
                               gTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACga                                                                                                           >  1:375320/1‑140 (MQ=12)
                               gTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACga                                                                                                           <  2:215688/141‑2 (MQ=12)
                               gTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACga                                                                                                           <  1:390324/141‑2 (MQ=17)
                                 aTGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATGTATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCACCGGTc                                                                                                         >  2:272464/1‑141 (MQ=17)
                                 aTGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTCAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTGCATCATTCAACgagc                                                                                                         >  2:18831/1‑138 (MQ=11)
                                  tGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCt                                                                                                        <  1:183971/141‑1 (MQ=32)
                                  tGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTAATCATTCAACGATCt                                                                                                        >  2:104592/1‑141 (MQ=255)
                                    aaCTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTAc                                                                                                      >  2:336597/1‑141 (MQ=255)
                                    aaCTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTAc                                                                                                      >  2:35818/1‑141 (MQ=12)
                                     aCTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTAcc                                                                                                     >  1:329370/1‑141 (MQ=32)
                                     aCTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTAcc                                                                                                     <  2:235791/141‑1 (MQ=32)
                                     aCTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTAcc                                                                                                     <  1:164302/141‑1 (MQ=12)
                                     aCTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTAcc                                                                                                     <  1:25732/141‑1 (MQ=12)
                                       tATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGt                                                                                                   <  2:347323/141‑1 (MQ=17)
                                        aTTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGtt                                                                                                  >  1:202139/1‑141 (MQ=17)
                                        aTTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGtt                                                                                                  <  2:148267/141‑1 (MQ=12)
                                        aTTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGtt                                                                                                  <  1:112106/141‑1 (MQ=255)
                                         tttCAGATATAAACAATTTGACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTg                                                                                                 >  1:192646/1‑141 (MQ=255)
                                         tttCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTg                                                                                                 <  2:61539/141‑1 (MQ=12)
                                         tttCAGATATAAACAACTTAACAAGGATGTTATCACTGTGTCTGTTGGCTACTATCTAAGATATGCATTTAGTTATCGTGATATATCTGAAATATTAAAGGAACGTGGTGTAAACGTTCATCATTATACGGTCTACCGTTg                                                                                                 >  2:333690/1‑141 (MQ=11)
                                           tCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTggg                                                                                               >  1:303746/1‑141 (MQ=255)
                                              gATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTc                                                                                            >  1:320881/1‑141 (MQ=17)
                                                tataAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCaa                                                                                          >  2:325139/1‑141 (MQ=17)
                                                tataAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCaa                                                                                          <  1:238224/141‑1 (MQ=255)
                                                tataAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGAGCTACCGTTGGGTTCaa                                                                                          <  2:275819/141‑1 (MQ=255)
                                                  ttaaCAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGGTATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGa                                                                                        <  2:22311/139‑1 (MQ=255)
                                                  aaaaCAATTGAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGTTTCAAGa                                                                                        <  1:250848/140‑1 (MQ=17)
                                                    aaCAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAat                                                                                      >  2:57507/1‑141 (MQ=12)
                                                      cAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAatat                                                                                    <  2:145166/141‑1 (MQ=32)
                                                       aaTTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATg                                                                                   <  2:321351/141‑1 (MQ=17)
                                                        aTTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAAAAATCTGCCTGTGGGCTCAAGAATATGc                                                                                  >  2:399919/1‑141 (MQ=11)
                                                         tttcacgcggATGTTATCACTGTAGCCGTGGGCTACTATCGAAGATATGCATTGAGTTATCGTGATATAGCTGAAAGAGTACGGGAACGTGGGGTAACCGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGcc                                                                                 <  1:29248/133‑1 (MQ=11)
                                                         tttAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGcc                                                                                 <  1:345371/141‑1 (MQ=255)
                                                           taccAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGcccc                                                                               <  1:224089/138‑1 (MQ=255)
                                                             aCAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCaa                                                                             >  1:256106/1‑141 (MQ=17)
                                                                  gATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCAAAtttat                                                                        >  2:373993/1‑139 (MQ=17)
                                                                   aggTTATCACTGTAGCGGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTtat                                                                       <  1:12666/139‑1 (MQ=21)
                                                                   aTGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATCTtat                                                                       <  1:271317/141‑1 (MQ=21)
                                                                    tGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTtata                                                                      <  2:300217/141‑1 (MQ=255)
                                                                      ttATCACTGTAGTCGTTTGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATc                                                                    >  2:36575/1‑141 (MQ=11)
                                                                      ttATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATc                                                                    >  1:60926/1‑141 (MQ=25)
                                                                      ttATCACTGTAGCAGATGGCTACGATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGATCATCATACAACCAACTACCGCTGGGTTCAAGAATATGCCCCAATTTTATATc                                                                    >  2:381706/1‑141 (MQ=11)
                                                                      gtagcACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATGTTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTAATATc                                                                    >  2:351269/5‑141 (MQ=11)
                                                                             tGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTg                                                             <  2:43093/141‑1 (MQ=25)
                                                                              gTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTgg                                                            >  2:123660/1‑141 (MQ=21)
                                                                              gTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTgg                                                            >  2:346026/1‑141 (MQ=34)
                                                                                atagaacgcctgctaTCTAAGATATGCATTGAGCAATCGTAGTATAACTGTAATATTAAGAGAAAGTGATGTAACCGTTCATAATTTAAAGATTTACTGTGTGGTTCATGAATATGCCCCTAGTTTATCTCAAATTTGgaa                                                          >  2:125635/13‑141 (MQ=255)
                                                                                 gctgTTTGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGgaag                                                         <  1:188729/138‑1 (MQ=25)
                                                                                 gCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGgaaa                                                         >  2:33027/1‑140 (MQ=25)
                                                                                   tgTTGGCTACTATCTAAGATCTGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGgaagaa                                                       <  1:142702/140‑1 (MQ=25)
                                                                                    gTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGaaa                                                      >  1:301555/1‑141 (MQ=255)
                                                                                       ggatacgaTTTAAGATATGCATTAATTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAg                                                   <  1:369604/138‑1 (MQ=11)
                                                                                       ggCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTGAACGTTCATCATTCAACGATCTACCGTTGGGTGCAAGAATATGCCCCAATTTTATATCAAATTTGGATGAGAAAg                                                   >  1:19740/1‑141 (MQ=11)
                                                                                        gCTACTATCGAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGc                                                  <  1:104935/141‑1 (MQ=25)
                                                                                         ctgctatctatGATATGCATTTAGCTATGCTGTTATATCTCAAATCTTCAGCGAACGGTCTGTAAACCTTCATCATTCAACGTTCTATCTGTCGGTTCCAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCa                                                 <  2:162368/138‑1 (MQ=2)
                                                                                         ctactaCTTAAGATATGCATGTAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAACAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCa                                                 <  1:34858/141‑1 (MQ=11)
                                                                                          tactaTTTAAAATAGTCATTAAGCTATCGTGATATATCTGAAAAAAAAAAAAAAAGTGGTGTAAACGTTCAACAGTCAACGATCTACCGTTCGGTTCAATAATATGCCCCAATTTTATATCATATTTGGaaaaaaaaaaaa                                                <  2:377094/141‑5 (MQ=255)
                                                                                          tactaTCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCAt                                                <  1:398335/141‑1 (MQ=35)
                                                                                          tactaTCTAAGATATGCATTGAGCTATCGTGATATATCTGATATATTAAGAGAACGTTGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCAt                                                <  2:290900/141‑1 (MQ=11)
                                                                                           actaTCTAAGATATGCATTGAGGTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGCTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCATa                                               <  2:258343/141‑1 (MQ=255)
                                                                                           actaTCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCATa                                               <  2:316209/141‑1 (MQ=255)
                                                                                            ctaTCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCATaa                                              >  1:132280/1‑141 (MQ=255)
                                                                                            ataTCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCATaa                                              <  1:247710/140‑1 (MQ=255)
                                                                                               tCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCATaagaa                                          <  1:162993/141‑1 (MQ=255)
                                                                                                cTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCATaaaaaa                                         <  1:51398/141‑2 (MQ=18)
                                                                                                 tAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATTCCCCAATTTTATATCAAATTTGGAAGAAAAAGCATaaaaaag                                        >  1:383382/1‑139 (MQ=11)
                                                                                                 tAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCATaaaaaag                                        <  2:292878/141‑3 (MQ=255)
                                                                                                      tatGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCATaaaaaagcttat                                   >  1:285797/1‑134 (MQ=11)
                                                                                                      tatGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCATAAACAAGCTGAt                                    >  2:276939/1‑141 (MQ=12)
                                                                                                      gatGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCATaaaaaagcttat                                   <  2:195363/140‑8 (MQ=1)
                                                                                                        tGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTAAACGGTCTACCGTTGGGTTCAAGAATATACCCCAATTTTATATCAAATTTGGAAGCAAAAGCATaatatagcttaata                                  >  1:50377/1‑129 (MQ=11)
                                                                                                              gAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCATAAAAAGCTAATTACAAATgg                           <  2:110978/141‑1 (MQ=39)
                                                                                                                       tGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCATAAAAAGCTAATTACAAATGGCGTATtggt                  >  1:595/1‑139 (MQ=38)
                                                                                                                       tGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCATAAAAAGCTAATTACAAATGGCGTATtgat                  >  1:362851/1‑141 (MQ=40)
                                                                                                                                tGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCATAAAAAGCTAATTACAAATGGCGTATTGATGAGACGTAc         >  1:228464/1‑141 (MQ=39)
                                                                                                                                            |                                                                                                                                        
ATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCATAAAAAGCTAATTACAAATGGCGTATTGATGAGACGTACATCAAAA  >  CP000730/76182‑76457

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: