Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 76,907 A→T 18.2% intergenic (+965/‑169) USA300HOU_0072 → / → USA300HOU_0075 universal stress protein/lysophospholipase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP00073076,9070AT18.2% 72.5 / 10.4 33intergenic (+965/‑169)USA300HOU_0072/USA300HOU_0075universal stress protein/lysophospholipase
Reads supporting (aligned to +/- strand):  ref base A (11/16);  new base T (2/4);  total (13/20)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.86e-01

TAAAAGGTATTGAATGTATTTACGCTCTATATAAAAAGAACCGCAGGTCTCTTCAGATCTACGGATTTTCGCCATGCCACGAAATTAGCATCATGCTAGCAAGTTAAGCGAACACTGACATGATAAATTAGTGGTTAGCTATATTTTTTTACTTTGCAACAGAACCTATAAAAAACATGGGCTTAAAGGGTTAATTTATATAGTATTACTGCAAACATTGATTTGGGTCAAAAATTAGGGGTATTAAAAAAATGAATGAATCATA  >  CP000730/76782‑77046
                                                                                                                             |                                                                                                                                           
tacaaGGGATTGAATGTACTTACGCTATTTATAAAAAGAAACGTAGGTCTCTTCAGATCTACGGATTTTCCCCATGACACGAAATTAGCATCATGCTAGCAAGTTAAACGAACACTGACATGATATATTAGTGATTAGCta                                                                                                                              <  1:119266/138‑1 (MQ=1)
     ggtcttgggtggaTTTACGCTCTATATAAAAAGAACCGCAGGTCTCTTCAGATCTACGGATTTTCGCCATGCCACGAAATTAGCATCATGCTAGCAAGTTAAACGAACACTGACATGATATATTAGTGATTAGCTATAttt                                                                                                                         >  2:344883/13‑141 (MQ=1)
            agtgcaTTTACGCTCTATATAAAAAGAACCGCACGTCTCTTCAGATCTACGGATTTTCGCCATGCCACGAAATTAGCATCATGCTAGCAAGTTAAACGAACACTGACATGATATATTAGTGATTAGCTATAttttttactt                                                                                                                  <  1:20127/136‑5 (MQ=1)
                  ttgaCGCTCTATATAAAAAGAACCGCAGGTCTCTTCAGATCTACGGATTTTCGCCATGCCACGAAATTAGCATCATGCTAGCAAGTTAAACGAACACTGACATGATATATTAGTGATTAGCTATATTTTTTACTTTGCAAc                                                                                                           >  2:111397/4‑141 (MQ=1)
                         tcgatataAAAATAACCGCAGGTCTCTTCAGATCTACGGATTTTCGCCATGCCACGAAATGAGCATCATGCTAGCAAGTTAAACGAACACTGACATGATATATTAGTGATTAGCTATATTTTTTACTTTGCAACAGAAcca                                                                                                    <  2:225532/138‑2 (MQ=1)
                         tccatctaAAAAGAACCGCAGGTCTCTTCACATCTTCGGATTTTGGCCATGCCACGAAATTAGCATCATGCTAGCAAGTTAAGCGAACACTGACATGATAAATTAGTGGTTAGCTATATTTTTTTACTTTGCAACAGAAcc                                                                                                     <  2:217049/135‑1 (MQ=1)
                         cctacagaaaaaGAACCGTAGGTCTCTTCAGATCTACGGATTTTCCCCATGCCACGAAATTAGCATCATGCTAGCAAGTTAAACGAACACTGACATGATATATTAGTGATTAGCTATATTTTTTACTTTGCAACAGAAcca                                                                                                    <  1:218019/134‑2 (MQ=1)
                           tatataAAAAGAACCGCAGGTCTCTTCAGATCTACGGATTTTCGCCATGCCACGAAATTAGCATCAGCCTAGCAAGTTAAGCGAACACTGACATGATAAATTAGTGGTTAGCTATATTTTTTTACTTTGCAACAGAACCta                                                                                                   >  2:162075/1‑141 (MQ=11)
                            atataAAAAGAACCGCAGGTCTCTTCAGATCTACGGATTTTCGCCATGCCACGAAATTAGCATCATGCTAGCAAGTTAAGCGAACACTGACATGATAAATTAGTGGTTAGCTATATTTTTTTACTTTGCAACAGAACCtat                                                                                                  >  1:145919/1‑141 (MQ=31)
                              ataAAAAGAACCGCAGGTCTCTTCAGATCTACGGATTTTCGCCATGCCACGAAATGAGCATCATGCTAGCAAGTTAAGCGAACACTGACATGATAAATTAGTGGTTAGCTATATTTTTTTACTTTGCAACAGAACCTATaa                                                                                                <  2:21387/141‑1 (MQ=17)
                                aaaaaGAACCGCAGGTCTCTTCAGATCTACGGATTTTCGCCATGCCACGAAATTAGCATCATGCTAGCAAGTTAAGCGAACACTGACATGATAAATTAGTGGTTAGCTATATTTTTTTACTTTGCAACAGAACCTATaaaa                                                                                              <  1:237099/141‑1 (MQ=34)
                                aaaaaGAACCGCAGGTCTCATCAGATCTACGGATTTTCGCCATGCCACGAAATTAGCATCATGCTAGCAAGTTAAGCGAACACTGACATGATAAATTAGTGGTTAGCTATATTTTTTTACTTTGCAACAGAACCTATaaaa                                                                                              >  1:238324/1‑141 (MQ=21)
                                 aaaaGAAACGCAGCTCTTTTCAGATCTACGGATGTTCGCTATGCCACGAAATTAGCACCATGATAGCAAGTTAAGCGAACACTGACATTATAAATTAGTGGTTAGCTATATTTTTTTACTTTGCAACAGAACCTATaaaaa                                                                                             <  2:86919/141‑1 (MQ=11)
                                       aCCGCAGGCCTCTTCAGATCTACGGATTTTCGCCATGCCACGAAATTAGCATCATGCTAGCAAGTTAAGCGAACACTGACATGATAAATTAGTGGTTAGCTATATTTTTTTACTTTGCAACAGAACCTATAAAAAACATgg                                                                                       <  2:347105/141‑1 (MQ=255)
                                         cGCAGGTCTCTTCAGATCTACGGATTTTCGCCATGCCACGAAATTAGCATCATGCTAGCAAGTTAAGCGAACACTGACATGATAAATTAGAGGTTAGCTATATTTTTTTACTTTGCAACAGAACCTATAAAAACCATGGGc                                                                                     >  1:303927/1‑141 (MQ=255)
                                          gCAGGTCTCTTCAGATCTACGGATTTTCGCCATGCCACGAAATTAGCATCATGCTAGCAAGTTAAGCGAACACTGACATGATAAATTAGTGGTTAGCTATATTTTTTTACTTTGCAACAAAACCTACAAAAAACATGGGCt                                                                                    <  1:213476/141‑1 (MQ=255)
                                          gCAGGTCTCTTCAGATCTACGGACTTTCGCCATGCCACGAAATTAGCATCATGCTAGCAAGTTAAGCGAACACTGACATGATAAATTAGTGGTTAGCTATATTTTTTTACTTTGCAACAGAACCTATAAAAAACATGGGCt                                                                                    <  2:107346/141‑1 (MQ=255)
                                              gTCTCTTCAGATCTACGGATTTTCGCCATGCCACGAAATTAGCATCATGCTAGCAAGTTAAGCGAACACTGACATGATAAATTAGTGGTTAGCTATATTTTTTTACTTTGCAACAGAACCTATAAAAAACATGGGCTTaaa                                                                                >  2:115110/1‑141 (MQ=255)
                                                   ttCAGATCTACGGATTTCTGCCATGCCACGAAATTAGCATCATGCTAGCAAGTTAAGCGAACACTGACATGATAAATTAGTGGTTAGCTATATTTTTTTACTTTGCAACAGAACCTATAAAAAACATGGGCTTAAAGGGtt                                                                           <  2:18655/141‑1 (MQ=255)
                                                   ctCAGAGCTACGGATTTTCGCCATGCCACGCAATTAGCATCATGCTAGCAAGTTAAGCGAACACTGACATGATAAATTAGTGGTTAGCTATATCTTTTTACTTTGCAACAGAACCTATAAAAAACATGGGCTTAAAGGGtt                                                                           <  2:256252/140‑1 (MQ=255)
                                                            gcgtgttttCGCCATGCCACGAAATTAGCATCATGCTAGCAAGTTAAGCGAACACTGACATGATAAATTAGTGGTTAGCTATATTTTTTTACTTTGCAACAGAACCTATAAAAAACATGGGCTTAAAGGGTTAATTtatat                                                                  <  2:195641/136‑1 (MQ=255)
                                                            aCGGATTTTCGCCATGCCACGAAATTAGCATCATGCTAGCAAGTTAAGCGAACACTGACATGATAAATTAGTGGTTAGCTATATTTTTTTACTTTGCAACAGAACCTATAAAAAACATGGGCTTAAAGGGTTAATTtatat                                                                  >  1:122281/1‑141 (MQ=255)
                                                             cGGATTTTCGCCATGCCACGAAATTAGCATCATGCTAGCAAGTTAAGCGAACACTGACATGATAAATTAGTGGTTAGCTATATTTTTTTACTTTGCAACAGAACCTATAAAAAACATGGGCTTAAAGGGTTAATTtatata                                                                 <  1:163463/141‑1 (MQ=255)
                                                                aTTTTCGCCATGCCACGAAATTAGCATCATGCTAGCAAGTTAAGCGAACACTGACATGATAAATTAGTGGTTAGCTATATTTTTTTACTTTGCAACAGAACCTATAAAAAACATGGGCTTAAAGGGTTAATTTATATAGTa                                                              >  1:171136/1‑141 (MQ=255)
                                                                aTTTTCGCCATGCCACGAAATTAGCATCATGCTAGCAAGTTAAGCGAACACTGACATGATAAATTAGTGGTTAGCTATATTTTTTTACTTTGCAACAGAACCTATAAAAAACATGGGCTTAAAGGGTTAATTTATATAGTa                                                              >  2:321990/1‑141 (MQ=255)
                                                                 ttttCGCCATGCCACGAAATTAGCATCATGCTAGCAAGTAAAGCGAACACTGACATGATAAATTAGTGGTTAGCTATATTTTTTTACTTTGCAACAGAACCTATAAAAAACATGGGCTTAAAGGGTTAATTTATATAGTAt                                                             <  2:50317/141‑1 (MQ=255)
                                                                     cGCCATGCCACGAAATTAGCATCATGCTAGCAAGTTAAGCGAACACTGACATGATAAATTAGTGGTTAGCTATATTTTTTTACTTTGCAACAGAACCTATAAAAAACATGGGCTTAAAGGGTTAATTTATATAGTATTACt                                                         >  2:398822/1‑141 (MQ=255)
                                                                              aCGAAAGTAGCATCATGCTAGCAAGTTAAGCGAACACTGACATGATAAATTAGTGGTTAGCTATATTTTTTTACTTTGCAACAGAACCTATAAAAAACATGGGCTTAAAGGGTTAATTTATATAGTATTACTGCAAACAtt                                                <  1:96047/141‑1 (MQ=255)
                                                                                         atcatGCTAGCAAGTTAAGCGAACACTGACATGATAAATTAGTGGTTAGCTATATTTTTTTACTTTGCAACAGAACCTATAAAAAACATGGGCTTAAAGGGTTAATTTATATAGTATTACTGCAAACATTGATTTGGGTCa                                     >  1:334940/1‑141 (MQ=255)
                                                                                          tcatGCTAGCAAGTTAAGCGAACACTGACATGATAAATTAGTGGTTAGCTATATTTTTTTACTTTGCAACAGAACCTATAAAAAACATGGGCTTAAAGGGTTAATTTATATAGTATTACTGCAAACAGTGATTTGGGTCaa                                    <  2:279530/141‑1 (MQ=255)
                                                                                                        tAAGCGAACACTGACATGATAAATTAGTGGTTAGCTATATTTTTTTACTTTGCAACAGAACCTATACAAAACATGGGCTTAAAGGGTTAATTTATATAGTATTACTGCAAACATTGATTTGGGTCAAAAATTAGAGGTAtt                      >  2:1362/1‑141 (MQ=255)
                                                                                                         aaGCGAACACTGACATGATAAATTAGTGGTTAGCTATATTTTTTTACTTTGCAACAGAACCTATAAAAAACATGGGCTTAAAGGGTTAATTTATATAGTATTACTGCAAACATTGATTTGGGTCAAAAATTAGGGGTATTa                     <  2:122281/141‑1 (MQ=255)
                                                                                                                  acgACATGATAAATTAGTGGTTAGCTATATTTTTTTACTTTGCAACAGAACCTATAAAAAACATGGGCTTAAAGGGTTAATCTATATAGTATTACTGCAAACATTGATTTGGGTCCAACATTAGGGGTATTAAAAAcatga            <  2:395580/139‑1 (MQ=255)
                                                                                                                          acaaaaTAGTGGTTAGAAATAATAAGATACGTTGCAACAGAACCTATAAAAAACATGGGCTTAAAGGGTTAATTTATATAGTATTACTGTAAACATAAAAAAAAAAAAAAAATTAGGGGTATTAAAAAAATGAATGAATCa    <  2:197230/139‑1 (MQ=255)
                                                                                                                            aaaTTAGTGGTTAGCTATATTTTTTTACTTTGCAACAGAACCTATAAAAAACATGGGCTTAAAGGGTTAATTTATATAGTATTACTGCAAACATTGATTTGGGTCAAAAATTAGGGGTATTAAAAAAATGAATGAATCATa  <  1:318955/141‑1 (MQ=255)
                                                                                                                            aaaTTAGTGGTTAGCTATATTTTTTTACTTTGCAACAGAACCTATAAAAAACATGGGCTTAAAGGGTTAATTTATATAGTATTACTGCAAACATTGATTTGGGTCAAAAATTAGGGGTATTAAAAAAATGAATGAATCATa  <  1:198534/141‑1 (MQ=255)
                                                                                                                             |                                                                                                                                           
TAAAAGGTATTGAATGTATTTACGCTCTATATAAAAAGAACCGCAGGTCTCTTCAGATCTACGGATTTTCGCCATGCCACGAAATTAGCATCATGCTAGCAAGTTAAGCGAACACTGACATGATAAATTAGTGGTTAGCTATATTTTTTTACTTTGCAACAGAACCTATAAAAAACATGGGCTTAAAGGGTTAATTTATATAGTATTACTGCAAACATTGATTTGGGTCAAAAATTAGGGGTATTAAAAAAATGAATGAATCATA  >  CP000730/76782‑77046

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: