Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 707,909 A→T 100% M1M (TTG→ATG) † USA300HOU_0653 ← ABC superfamily ATP binding cassette transporter, ABC protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP000730707,9090AT100.0% 107.5 / ‑6.5 36M1M (TTG→ATG) †USA300HOU_0653ABC superfamily ATP binding cassette transporter, ABC protein
Reads supporting (aligned to +/- strand):  ref base A (1/0);  new base T (18/17);  total (19/17)
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

ACTTGATAAGGGAAGATTTACCAGCACCATTCGGGCCCATGATACCAATTATTTCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACAAACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCGTGATATAATATAGACAATGTATGTGAGGTGAAAGTATGTTAACTGAAGAA  >  CP000730/707771‑708045
                                                                                                                                          |                                                                                                                                        
aCTTGATAAGGGAAGATTTACCAGCACCATTCGGGCCCATGATACCAATTATTTCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACATAc                                                                                                                                        >  1:348377/1‑141 (MQ=255)
   tGATAAGGGAAGATTTACCAGCACCATTCGGGCCCATGATACCAATTATTTCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACATACGtt                                                                                                                                     <  1:211280/141‑1 (MQ=255)
        aGGGAAGATTTACCAGCACCATTCGGGCCCATGATACCAATTATTTCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACATACGTTTATAc                                                                                                                                >  2:26804/1‑141 (MQ=255)
         gggAAGATTTACCAGCACCATTCGGGCCCATGATACCAATTATTTCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTGTAACATACGTTTATAcc                                                                                                                               >  1:54180/1‑141 (MQ=255)
         gggAAGATTTACCAGCACCATTCGGGCCCATGATACCAATTATTTCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACATACGTTTATAcc                                                                                                                               >  2:43955/1‑141 (MQ=255)
              gATTTACCAGCACCATTCGGGCCCATGATACCAATTATTTCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACATACGTTTATAcctcctc                                                                                                                          <  2:351110/141‑2 (MQ=255)
                  tACCAGCACCATTCGGGCCCATGATACCAATTATTTCGCCGCGTACTGCTACCGATAAGGAAATGGTTTTAAGTACATACTTATTACCTAAAAACAGAGTTAAATCTTTTGTTTCTACCAAACGTTTATCACTCCTAATTa                                                                                                                      >  2:78129/1‑141 (MQ=255)
                        caccacTCGGGCCCAAGACACCAATTATTTCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATAAAAAAAAAAAAAAAACAGATTTAAATCTTTTGTTTCTAACATACGTTTATACCTCCTAATTAAAAGtt                                                                                                                <  2:378204/141‑1 (MQ=255)
                        cACCATTCGGGCCCATGATACCAATTATTTCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACATACGTTTATACCTCCTAATTAAAAGtt                                                                                                                <  2:348377/141‑1 (MQ=255)
                         aCCATTCGGGCCCATGATACCAATTATTTCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACATACGTTTATACCTCCTAATTAAAAGttt                                                                                                               >  1:97718/1‑141 (MQ=255)
                                 ggCCTATGATTCCAATTATTTCGCCGCGTACTGGTAACGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACATACGTTTATACCTCCTAATTAAAAGTTTAGGCTAAc                                                                                                       <  2:334279/141‑1 (MQ=255)
                                  gCCCATGATACCAATTATTTCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACATACGTTTATACCTCCTAATTAAAAGTTTAGGCTAAcc                                                                                                      <  1:338933/141‑1 (MQ=255)
                                    ccATGATACAAATTATTAAGCCAGGTACAGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACATACGTTTGTGCCGCCTAATTAAAAGTTTAGGCTAACCta                                                                                                    >  2:82495/1‑141 (MQ=255)
                                             cAATTATTTCGCCGCGTACTGGCATCGATAAGGAAATGTTTTTAAGGCCATGCTTATTACCTAACAACGGATTTAAATCTGTTGTGTCTACCATACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTaattaattat                                                                                           >  1:235440/1‑139 (MQ=255)
                                                       gccgcGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACATACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAGTAAACt                                                                                 >  2:314152/1‑141 (MQ=255)
                                                                   gaTCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACATACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTAt                                                                     <  1:151242/140‑1 (MQ=255)
                                                                     tCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACATACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCa                                                                   <  2:54180/141‑1 (MQ=255)
                                                                       gATAAGGAAATGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACATACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCAtt                                                                 >  2:372585/1‑140 (MQ=255)
                                                                             gAAATGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACATACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGt                                                           <  1:26804/141‑1 (MQ=255)
                                                                             gAAATGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACATACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGt                                                           <  2:97718/141‑1 (MQ=255)
                                                                              aaaTGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACATAAGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTa                                                          >  1:327331/1‑141 (MQ=255)
                                                                                aTGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACATACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTaaa                                                        >  2:245868/1‑141 (MQ=255)
                                                                                    ttttAAGTATATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACATACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCg                                                    <  2:134621/141‑1 (MQ=255)
                                                                                          gTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACATACGTTTATACATCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTAAAGTAAATTCGTGatat                                              >  2:163785/1‑141 (MQ=255)
                                                                                               ttcTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACATACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCGTGatataatat                                         <  2:369086/139‑1 (MQ=255)
                                                                                                       aCCTAAAAACAGATTTAAATCTTTTGTTTCTAACATACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCGTGATATAATATAGACAatg                                 <  1:325204/141‑1 (MQ=255)
                                                                                                            aaaaCAGATTTAAATCTTTTGTTTCTAACATACGTTTATACCTCCTTATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCGTGATATAATATAGACAatgtatgt                            <  1:367572/141‑1 (MQ=255)
                                                                                                            aaaaCAGATTTAAATCTTTTGTTTCTAACATACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCGTGATATAATATAGACAatgtatgt                            <  1:314845/141‑1 (MQ=255)
                                                                                                             aaaCAGATTTAAATCTTTTGTTTCTAACATACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCGTGATATAATATAGACAATGTAtgtg                           >  1:155056/1‑141 (MQ=255)
                                                                                                             aaaCAGATTTAAATCTTTTGTTTCTAACATACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCGTGATATAATATAGACAATGTAtgtg                           >  1:247620/1‑141 (MQ=255)
                                                                                                               aCAGATTTAAATCTTTTGTTTCTAACATACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCGTGATATAATATAGACAATGTATGTGAg                         >  2:320893/1‑141 (MQ=255)
                                                                                                                cAGATTTAAATCTTTTGTTTCTAACATACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCGGAATATAATATAGACAatggtagtgggg                        >  2:44967/1‑132 (MQ=255)
                                                                                                                        aaTCTTTTGTTTCTAACATACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCGTGATATAATATAGACAATGTATGTGAGGTGAAAGTa                >  1:41968/1‑141 (MQ=255)
                                                                                                                            ttttGTTTCTAACATACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCGTGATATAATATAGACAATGTATGTGAGGTGAAAGTATGtt            >  1:123208/1‑141 (MQ=255)
                                                                                                                             tttGTTTCTAACATACGTTTATACCTCCTAATTAAAAGCTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATCTCAAGTAAATTCGTGATATAATATAGACAATGTACGTGAGGTGAAAGTATGTTa           <  1:259170/141‑1 (MQ=255)
                                                                                                                                      aaCATACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCGTGATATAATATAGACAATGTATGTGAGGTGAAAGTATGTTAACTgaagaa  <  2:203349/141‑1 (MQ=255)
                                                                                                                                          |                                                                                                                                        
ACTTGATAAGGGAAGATTTACCAGCACCATTCGGGCCCATGATACCAATTATTTCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACAAACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCGTGATATAATATAGACAATGTATGTGAGGTGAAAGTATGTTAACTGAAGAA  >  CP000730/707771‑708045

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: