Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 1,105,994 T→C 100% T12T (ACT→ACC pdhD → dihydrolipoyl dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007301,105,9940TC100.0% 78.2 / ‑6.5 26T12T (ACT→ACCpdhDdihydrolipoyl dehydrogenase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base C (11/13);  minor base A (1/0);  total (12/14)
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

TGACCACAGACAAATTGATGGTGCAACTGGCCAAAATGCAATGAATCACATTAAACGTTTATTAAATAATCCAGAATTATTATTAATGGAGGGGTAAAACATGGTAGTTGGAGATTTCCCAATTGAAACAGATACTATAGTAATCGGAGCAGGTCCTGGTGGATACGTTGCAGCAATTCGTGCAGCTCAATTAGGACAAAAAGTAACAATCGTTGAGAAAGGTAATCTTGGTGGTGTTTGCTTAAACGTAGGATGTATTCCTT  >  CP000730/1105859‑1106121
                                                                                                                                        |                                                                                                                               
tGACCACAGACAAATTGATGGTGCAACTGGCCAAAATGCAATGAAGCACATTAAACGTTTATTAAATAATCCAGAATTATTATTAATGGAGGGGTAAAACATGGTAGTTGGAGATTTCCCAATTGAAACAGATACCATAga                                                                                                                            >  1:376216/1‑140 (MQ=255)
 gACCACAGACAAATTGCTGGTGCAACTGGCCAAAACGCAATGAATCACATTAAACGTTTATTAAATAATCCAGAATTATTATTAATGGAGGGGTAAAACATGGTAGTTGGAGATTTCCCAATTGAAACAGATACCATAGTa                                                                                                                           <  1:117187/141‑1 (MQ=255)
      caGACAAATTGATGGTGCAACTGGCCAAAATGCAATGAATCACATTAAACGTTTATTAAATAATCCAGAATTATTATTAATGGAGGGGTAAAACATGGTAGTTGGAGATTTCCCAATTGAAACAGATACCATAGTAATCgg                                                                                                                      >  1:13028/1‑141 (MQ=255)
      aaGACAAATTGATGGTGCAACTGGCCAAAATTCAATGAATCACATTAAACGTTTATTAAATAATCCAGAATTATTATTAATGGAGGGGTAAAACATGGTAGTTGGAGATTTCCCAATTGAAACAGATACCATAGTAATCgg                                                                                                                      <  2:261139/140‑1 (MQ=255)
          cAAATGGATGGGGCACCTGGACAAAATGCAATGAATCACATTAAACGTTTATAAAATAATCCAGAATTCTTATTAATGGAGGGGTAAAACATGGTAGTTGGAGATTTCCCAATTGAAACAGATACCAGAGTAATAGGGGCa                                                                                                                  >  2:11617/1‑141 (MQ=255)
                              ccAAAATGCAATGAATCACATTAAAAGTTTATTAAATAATCCAGAATTATTATTAATGCAGGGGTAAAACATGGTAGTTGGGGATTTCCCAATTGAAACAGATACCATAGTAATCTGAGCAAGTCCTGGGGgttgcgttgc                                                                                              >  2:58169/1‑141 (MQ=255)
                                   aTGCAATGAATCACATTAAACGTTTATTAAATAATCCAGAATTATTATTAATGGAGGGGTAAAACATGGTAGTTGTAGATTTCCTTAGGGAGACAGATACCATAGTAATCGGAGCAGGTCCTGGTGGATACGTTGCAGCaa                                                                                         <  1:93517/141‑1 (MQ=255)
                                      cAATGAATCACATTAAACGTTTATTAAATAATCCAGAATTATTATTAATGGAGGGGTAAAACATGGTAGTTGGAGATTTCCCAATTGAAACAGATACCATAGTAATCGGAGCAGGTCCTGGTGGATACGTTGCAGCAATTc                                                                                      <  1:396324/141‑1 (MQ=255)
                                            aTCACATTAAACGTTTATTAAATAATCCAGAATTATTATTAATGGAGGGGTAAAACATGGTAGTTGGAGATTTCCCAATTGAAACAGATACCATAGTAATCGGAGCAGGTCCTGGTGGATACGTTGCAGCAATTCGTGCAg                                                                                <  2:281924/141‑1 (MQ=255)
                                              cacaATAACCATTTATTAAATAATCAAGACTTATTATTAATGGACGGGTAAAATATGGTCGATGGAGATTTCACAATTGAAACAGAGACAGTAGTAATCGGGCCAGGTCCTGGTGGATACGTTGCAGGAATTCGTGAAGca                                                                              >  2:102192/1‑140 (MQ=255)
                                                        gTTTATTAAATAATCCAGAATTATTATTAATGGAGGGGTAAAACATGGTAGTTGGAGATTTCCCAATTGAAACAGATACCATAGTAATCGGAGCAGGTCCTGGTGGATACGTTGCAGCAATTCGTGCAGCTCAATTAGGAc                                                                    <  1:113837/141‑1 (MQ=255)
                                                            attaAATAATCCAGAATTATTATTAATGGAGGGGTAAAACATGGTAGTTGGAGATTTCCCAATTGAAACAGATACCATAGTAATCGGAGCAGGTCCTGGTGGATACGTTGCAGCATTTCGTGCAGCGCAATTAGGACaaaa                                                                <  1:218474/141‑1 (MQ=255)
                                                             ttaAATAATCCAGAATTATTATTAATGGAGGGGTAAAACATGGTAGTTGGAGATTTCCCAATTGAAACAGATCCCATAGTAATCGGAGCAGGTCCTGGTGGATACGTTGCAGCAATTCGTGCAGCTCAATTAGGACaaaaa                                                               >  1:176032/1‑141 (MQ=255)
                                                                  taatCCAGAATTATTATTAATGGAGGGGTAAAACATGGTAGTTGGAGATTTCCCAATTGAAACAGATACCATAGTAATCGGAGCAGGTCCTGGTGGATACGTTGCAGCAATTCGTGCAGCTCAATTAGGACAAAAAGTAAc                                                          <  1:336695/141‑1 (MQ=255)
                                                                  aaatCCAGAATTATTATTAATGGAGGGGTAAAACATGGTAGTTGGAGATTTCCCAATTGAAACAGATACCATAGTAATCGGAGCAGGTCCTGGTGGATACGTTGCAGCAATTCGTGCAGCTCAATTAGGACAAAAAGTAAc                                                          <  2:298344/140‑1 (MQ=255)
                                                                                tattaATGGAGGGGTAAAACATGGTAGTTGGAGATTTCCCAATTGAAACAGATACCATAGTAATCGGAGCAGGTCCTGGTGGATACGTTGCAGCAATTCGTGCAGCTCAATTAGGACAAAAAGTAACAATCGTTGAGAAAg                                            >  1:7812/1‑141 (MQ=255)
                                                                                            ggTAAAAACTGGTATTTTGAGAGTGCCAAATTGAACCAGAGTACATAGTAATCGGATTAGGTGCTGGTGGATACGCTGCAGCAACTCGTGCAGGGCAATTAGTACAACAATTAACAATCGTTGAGAAAGGTAATCTtggtg                                <  2:170318/141‑1 (MQ=255)
                                                                                              tAAAACATGGTAGTTGGAGATTTCCCAATTGAAACAGATACCATAGTAATCGGAGCAGGTCCTGGTGGATACGTTGCAGCAATTCGTGCAGCTCAATTAGGACAAAAAGTAACAATCGTTGAGAAAGGTAATCTtggtggg                              >  1:360744/1‑140 (MQ=255)
                                                                                              tAAAACATGGTAGTTGGAGATTTCCCAATTGAAACAGATACCATAGTAATCGGAGCAGGTCCTGGTGGATACGTTGCAGCAATTCGTGCAGCTCAATTAGGACAAAAAGTAACAATCGTTGAGAAAGGTAATCTTggtggt                              >  1:98679/1‑141 (MQ=255)
                                                                                              tAAAACATGGTAGTTGGAGATTTCCCAATTGAAACAGATACCATAGTAATCGGAGCAGGTCCTGGAGGATACGTTGCAGCAATTCGTGCAGCTCAATTAGGACAAAAAGTAAAAATCGTTGAGAAAGGTAATCTTggtggt                              >  1:174238/1‑141 (MQ=255)
                                                                                               aaaaCATGGTAGGTGGAGATTTCTCAAGTGAGATAGATACCATAGTAATCGGAGCAGGTCCTGGTGGATACGTTGCAGCAAGTCGTGCAACTCAATTAGGACAAAAAGTAACAATCGTGGAGAAAGGTACTCTTGgcggtg                             >  2:264062/1‑141 (MQ=255)
                                                                                                      gatacttGGATATTTAACAATTGAAACAGATAACATAGTAATCGGAGCAGGTCCTGGTGGATAAGTTGCAGCAATTAGTGCAGCACAATTAGGACAAAAAGTAACAATCGTTGAGAAAGGTAATCTTGGTGGTGTTTGCtt                      <  2:288861/136‑1 (MQ=255)
                                                                                                               agaTTTCCCAGTTGAAACAGATGCCATAGAAATCGGAGCAGGTCCTGGTGGATACGTTGCAGCAATTCGTGCAGCTATATTAGGACAAAAAGTAACAATCGTTGAGAAAGGTAATCTTGGTGGTGTTTGCTTAAACGTAgg             <  2:13028/141‑1 (MQ=255)
                                                                                                                 aTTTCCCAATTGAAACAGATACCATAGTAATCGGAGCAGGTCCTGGTGGATACGTTGCAGCAATTCGTGCAGCTCAATTAGGACAAAAAGTAACAATCGTTGAGAAAGGTAATCTTGGTGGTGTTTGCTTAAACGTAGGAt           >  1:61856/1‑141 (MQ=255)
                                                                                                                     cccAATTGAAACAGATACCATAGTAATCGGAGCAGGTCCTGGTGGATACGTTGCAGCAATTCGTGCAGCTCAATTAGGACAAAAAGTAACAATCGTTGAGAAAGGTAATCTTGGTGGTGTTTGCTTAAACGTAGGATGTAt       <  2:323396/141‑1 (MQ=255)
                                                                                                                          ttGAAACAGATACCATAGTAATCGGAGCAGGTCCTGGTGGGTACGTTGCAGCAATTCGTGCAGCTCAATTAGGACAAAAAGTAACAATCGTTGAGAAAGGTAATCTTGGTGGTGTTTGCTTAAACGTAGGATGTATTCCtt  <  1:226537/141‑1 (MQ=255)
                                                                                                                                        |                                                                                                                               
TGACCACAGACAAATTGATGGTGCAACTGGCCAAAATGCAATGAATCACATTAAACGTTTATTAAATAATCCAGAATTATTATTAATGGAGGGGTAAAACATGGTAGTTGGAGATTTCCCAATTGAAACAGATACTATAGTAATCGGAGCAGGTCCTGGTGGATACGTTGCAGCAATTCGTGCAGCTCAATTAGGACAAAAAGTAACAATCGTTGAGAAAGGTAATCTTGGTGGTGTTTGCTTAAACGTAGGATGTATTCCTT  >  CP000730/1105859‑1106121

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: