Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 938,546 G→T 17.5% intergenic (+1453/‑564) USA300HOU_0902 → / → USA300HOU_0903 NADH dehydrogenase/NhaC family sodium:proton (Na+:H+) antiporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP000730938,5460GT17.5% 52.5 / 5.6 27intergenic (+1453/‑564)USA300HOU_0902/USA300HOU_0903NADH dehydrogenase/NhaC family sodium:proton (Na+:H+) antiporter
Reads supporting (aligned to +/- strand):  ref base G (12/10);  new base T (2/3);  total (14/13)
Fisher's exact test for biased strand distribution p-value = 6.48e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.90e-01

ATATATTACAAATAAGTTCTGAAGTCGATGAAATGGTATTTGAATTACCGATTACTGCAACCGAACGTGCAAGTATTAAACACAGTGATATCGCTGATTTAGTTAACCATACGAATGGACAAGGTAAAGCGCTATTTGCGGCAAGTTTTGTAACACATTTTAGTGGTCAAACACCTCACATTCATTTCGATATTGCAGGTCCAGCAACGACTAATAAAGCTTCATATAATGGTCCAAAAGGGCCAACAGGATTTATGATTCCGACGATAGTACAATGG  >  CP000730/938407‑938684
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atatatTACAAATAAGTTCTGAAGTCGATGAAATGGTATTTGAATTACCGATTACTGCAACCGAACGTGCAAGTATTAAACACAGTGATATCGCTGATTTAGTTAACCATACGAATGGACAAGGTAAAGCGCTATTTGCgg                                                                                                                                           >  1:27653/1‑141 (MQ=255)
              aGTTCTGAAGTCGATGAAATGGTATTTGAATTACCGATTACTGCAACCGAACGTGCAAGTATTAAACACAGTGATATCGCTGATTTAGTTAACCATACGAATGGACAAGGTAAAGCGCTATTTGCGGCAAGTTTTGTAaca                                                                                                                             >  1:215446/1‑141 (MQ=255)
                 tCTGAAGTCGATGAAATGGTATTTGAATTACCGATTACTGCAACTGAACGTGCAAGTATTAAACACAGTGATATCGCCGATTTAGCTAACCATACGAATGGCCAAGGCACAGCGCTCTTTGCGACAAGTTTTGTAACACAt                                                                                                                          >  2:148564/1‑141 (MQ=255)
                  cTGAAGTCGATGAAATGGTATTTGAATTACCGATTACTGCAACCGAACGTGCAAGTATTAAACACAGTGATATCGCTGATTTAGTTAACCATACGAATGGACAAGGGAAAGCGCGATTTGCGGCAAGTTTTGTAACACAtt                                                                                                                         >  1:85819/1‑141 (MQ=255)
                        tCGATGAAATGGTATTTGAATTACCGATTACTGCAACCGAACGTGCAAGTATTAAACACAGTGATATCGCTGATTTAGTTAACCATACGAATGGACAAGGTAAAGCGCTATTTGCGGCAAGTTTTGTAACACATTTTAGTg                                                                                                                   <  1:178006/141‑1 (MQ=255)
                                    tATTTGAATTACCGATTACTGCAACCGAACGTGCAAGTATTAAACACAGTGATATCGCTGATTTAGTTAACCATACGAATGGACAAGGTAAAGCGCTATTTGCTGCAAGTTTTGTAACACATTTTAGTGGTCAAACACCTc                                                                                                       >  2:68539/1‑141 (MQ=255)
                                    tATTTGAATTACCGATTACTGCAACCGAACGTGAAAGTATTAAACACAGTGATATCGCTGATTTAGTTAACCATACGAATGGGCAAGGTAAAGCGCTATTTGCTGCAAGTTTTGTAACACATTTTAGTGGTCAAACACCTc                                                                                                       >  2:178405/1‑141 (MQ=255)
                                                      cTGCAACCGACCGTGCAAGTATTAAACACACTGATATCGCTGATTTAGTTAACCATACGAATGGACAAGGTAAAGCGCTATTTGCGGCAAGTTTTGTAACACATTTTAGTGGTACAACACCACACGTTCCTTTCGCTAttc                                                                                     >  2:246852/1‑140 (MQ=255)
                                                         cAACCGAACGTGCAAGTATTAAACACAGTGATATCGCTGATTTAGTTAACCATACGAATGGACAAGGTAAAGCGCTATTTGCGGCAAGTTTTGTAACACATTTTAGTGGTCAAACACCTCACATTCATTTCGATATTGCAg                                                                                  >  2:18113/1‑141 (MQ=255)
                                                              gAACGTGCAAGTATTAAACACAGTGATATCGCTGATTTAGTTAACCATACGAATGGACAAGGTAAAGCGCTATTTGCGGCAAGTTTTGTAACACATTTTAGTGGTCAAACACCTCACATTCATTTCGATATTGCAGGTCCa                                                                             <  1:164930/141‑1 (MQ=255)
                                                              gAACGTGCAAGTATTAAACACAGTGATATCGCTGATTTAGTTAACCATACGAATGGACAAGGTAAAGCGCTATTTGCGGCAAGTTTTGTAACACATTTTAGTGGTCAAACACCTCACATTCATTTCGATATTGCAGGTCCa                                                                             <  1:36097/141‑1 (MQ=255)
                                                                       aGTATTAAACACGGTGATATCGCTGATTTAGTTAACCATACGAATGGACAAGGTAAAGCGCTATTTGCGGCAAGTTTTGTAACACATTTTAGTGGTCAAACACCTCACATTCATTTCGATATTGCAGGTCCAGCAACGACt                                                                    <  2:75668/141‑1 (MQ=255)
                                                                          aTTAAACACAGTGATATCGCTGATTTAGTTAACCATACGAATGGACAAGGTAAAGCGCTATTTGCTGCAAGTTTTGTAACACATTTTAGTGGTCAAACACCTCACATTCATTTCGATATTGCAGGTCCAGCAACGACtaat                                                                 <  1:178405/141‑1 (MQ=255)
                                                                          aTTAAACACAGTGATATCGCTGATTTAGTTAACCATACGAATGGACAAGGTAAAGCGCTATTTGCTGCAAGTTTTGTAACACATTTTAGTGGTCAAACACCTCACATTCATTTCGATATTGCAGGTCCAGCAACGACtaat                                                                 <  1:68539/141‑1 (MQ=255)
                                                                              aaCACAGTGATATCGCTGATTTAGTTAACCATACGAATGGACAAGGTAAAGCGCTATTTGCGGCAAGTTTTGTAACACATTTTAGTGGTCAAACACCTCACATTCATTTCGATATTGCAGGTCCAGCAACGACTAATAAAg                                                             <  1:18113/141‑1 (MQ=255)
                                                                                             cTGATTTAGTTAACCATACGAATGGACAAGGTAAAGCGCTATTTGCGGCAAGTTTTGTAACACATTTTAGTGGTCAAACACCTCACATTCATTTCGATATTGCAGGTCCAGCAACGACTAATAAAGCTTCATATAATGGTc                                              >  1:132718/1‑141 (MQ=255)
                                                                                                aTTTAGTTAACCATACGAAAGGACAAGGTAAAGCGCTATTTGCGGCAAGTTTTGTAACACATTTTAGTGGTCAAACACCTCACATTCATTTCGATATTGCAGGTCCAGCAACGACTAATAAAGCTTCAAATAATGGTCCaa                                           >  2:85435/1‑141 (MQ=255)
                                                                                                         aCCATACGAATGGACAAGGTAAAGCGCTATTTGCGGCAAGTTTTGTAACACATTTTAGTGGTCAAACACCTCACATTCATTTCGATATTGCAGGTCCAGCAACGACTAATAAAGCTTCATATAATGGTCCAAAAGGGCCaa                                  >  1:269848/1‑141 (MQ=255)
                                                                                                                ggctGGACAAGGTAAACTGCTATTTGCTGCAAGTTTTGTAACACATTCTAGTGGTCAACCACCTCACATTCATTTCGATATTGCAGTTCCAGCAACGACTAATAAAGCTTCATATAATGGTCCAAAAGGGCCAACAGGAtt                           <  2:185323/138‑1 (MQ=255)
                                                                                                                 aaTGGACAAGGTAAAGCGCTATTTGCGGCAAGTTTTGTAACACATTTTAGTGGTCAAACACCTCACATTCATTTCGATATTGCAGGTCCAGCAACGACTAATAAAGCTTCTTATAATGGTCCAAAAGGGCCAACAGGAttt                          >  1:2674/1‑141 (MQ=255)
                                                                                                                 aaTGGACAAGGTAAAGCGCTATTTGCGGCAAGTTTTGTAACACATTTTAGTGGTCAAACACCTCACATTCATTTCGATATTGCAGGTCCAGCAACGACTAATAAAGCTTCATATAATGGTCCAAAAGGGCCAACAGGAttt                          >  2:97665/1‑141 (MQ=255)
                                                                                                                 aaTGGACAAGGTAAAGCGCTATTTGCGGCAAGTTTTGTAAAACATTTTAGTGGTCAAACACCTCACATTCATTTCGATATTGCAGGTCCAGCAACGACTAATAAAGCTTCATATAATGGTCCAAAAGGGCCAACAGGAttt                          >  2:176036/1‑141 (MQ=255)
                                                                                                                   tGGACAAGGGAAAGCGCTATTTGCGGCAAGGTTTGTAACACATTTTAGTGGTCAAACACCTCACATTCATTTCGATATTGCAGGTCCAGCAACGACTAATAAAGCTTCATATAATGGTCCAAAAGGGCCAACAGGATTTAt                        <  1:113483/141‑1 (MQ=255)
                                                                                                                      aCAAGGTAAAGCGCTTTTTGCGGCAAGTTTTGTAACACATTTTAGTGGTCAAACACCTCACATTCATTTCGATATTGCAGGTCCAGCAACGACTAATAAAGCTTCATATAATGGTCCAAAAGGGCCAACAGGATTTATGAt                     <  2:266298/141‑1 (MQ=255)
                                                                                                                            tAAAGCGCTATTTGCGGCAAGTTTTGTAACACATTTTAGTGGTCAAACACCTCACATTCATTTCGATATTGCAGGTCCAGCAACGACTAATAAAGCTTCATATAATGGTCCAAAAGGGCCAACAGGATTTATGATTCcgaa               >  2:147643/1‑140 (MQ=255)
                                                                                                                                gcgcTATTTGCGGCAAGTTTTGTAACACATTTTAGTGGTCAAACACCTCACATTCATTTCGATATTGCAGGTCCAGCAACGACTAATAAAGCTTCATATAATGGTCCAAAAGGGCCAACAGGATTTATGATTCCGACGATa           <  1:147643/141‑1 (MQ=255)
                                                                                                                                       ttGCGGGAAGTTTTGTAACACATTTTAGTGGTCAAACACCTCACATTCATTTCGATATTGCAGGTCCAGCAACGACTAATAAAGCTTCATATAATGGTCCAAAAGGGCCAACAGGATTTATGATTCCGACGATAGTACaaa    <  2:27653/141‑2 (MQ=255)
                                                                                                                                         gCGGCAAGTTTTGAAACACATTTTAGTGGTCAAACACCTCACATTCATTTCGATAGTGCAGGTCCAGCAACGACTAATAAAGCTTCATATAATGGGCCAAAAGGGCCAACAGGATTTATGATTCCGACGATAGTACAATgg  <  1:46109/141‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                          
ATATATTACAAATAAGTTCTGAAGTCGATGAAATGGTATTTGAATTACCGATTACTGCAACCGAACGTGCAAGTATTAAACACAGTGATATCGCTGATTTAGTTAACCATACGAATGGACAAGGTAAAGCGCTATTTGCGGCAAGTTTTGTAACACATTTTAGTGGTCAAACACCTCACATTCATTTCGATATTGCAGGTCCAGCAACGACTAATAAAGCTTCATATAATGGTCCAAAAGGGCCAACAGGATTTATGATTCCGACGATAGTACAATGG  >  CP000730/938407‑938684

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: