| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP000730 | 1,232,052 | G→T | 100% | D233Y (GAT→TAT) | USA300HOU_1156 → | possible PP2C protein phosphatase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP000730 | 1,232,052 | 0 | G | T | 100.0% | 47.8 / NA | 17 | D233Y (GAT→TAT) | USA300HOU_1156 | possible PP2C protein phosphatase |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base T (7/10); total (7/10) | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
ATTCAGATGGATTAACTGATTATGTTAAAGACAATGAAATTAAGCGTTTGTTAGTAAAAGAAGGTACAATAGAAGATCATGGTGATCAATTAATGCAATTGGCATTAGATAACCATTCGAAAGATAACGTTACTTTCATACTCGCGGCTATTGAAGGTGATAAAGTATGATAGGTAAAATAATAAATGAACGATATAAAATTGTAGATAAGCTTGGCGGCGGTGGCATGAGTACCGTTTATCTTGCTGAAGATACGA > CP000730/1231930‑1232186 | atacAGATGGATTAACTGATTATGTTAAAGACAATGAAATTAAGCGTTTGTTAGTAAAAGAAGGTACAATAGAAGATCATGGTGATCAATTAATGCAATTGGCATTAGATAACCATTCGAAATATAACGTTACTTTCATAc < 2:138616/138‑1 (MQ=255) aTGGATTAACTGATTATGTTAAAGACAATGAAATTAAGCGTTTGTTAGTAAAAGAAGGTACAATAGAAGATCATGGTGATCAATTAATGCAATTGGCATTAGATAACCATTCGAAATATAACGTTACTTTCATACTCGCgg < 2:21571/141‑1 (MQ=255) tATGTTAAAGACAATGAAATTAAGCGTTTGTTAGTAAAAGAAGGTACAATAGAAGATCATGGTGATCAATTAATGCAATTGGCATTAGATAACCATTCGAAATATAACGTTACTTTCATACTCGCGGCTATTGAAGGTggt > 2:269116/1‑139 (MQ=255) tAAAGACAATGAAATTAAGCGTTTGTTAGTAAAAGAAGGTACAATAGAAGATCATGGTGATCAATTAATGCAATTGGCATTAGATAACCATTCGAAATATAACGTTACTTTCATACTCGCGGCTATTGAAGGTGATAAAGt > 1:204894/1‑141 (MQ=255) aGACAATGAAATTAAGCGTTTGTTAGTAAAAGAAGGTACAATAGAAGATCATGGTGATCAATTAATGCAATTGGCATTAGATAACCATTCGAAATATAGCGTTACTTTCATACTAGCGGCGATTTAAGGTGATACAGTATg > 2:107520/1‑141 (MQ=255) aaGCGTTTGTTAGTAAAAGAAGGTACAATAGAAGATCATGGTGATCAATTAATGCAATTGGCATTAGATAACCATTCGAAATATAACGTTACTTTCATACTCGCGGCTATTGAAGGTGATAAAGTATGATAGGTAAaataa < 1:2540/141‑1 (MQ=255) ttAGTAAAAGAAGGTACAATCGAAGATCATGGTGATCAATTAATGCAATTGGCATTAGATAACCATTCGAAATATAACGTTACTTTCATACTCGCGGCTATTGAAGGTGATAAAGTATGATAGGTAAAATACTAAATGAAc < 2:173782/141‑1 (MQ=255) aaaaaGAAGGTACAATAGAAGGTCATGGTGATCAATTAATGCAATTGGCATTAGATAACCATTCGAAATATAACGTTACTTTCATACTCGCGGCTATTGAAGGTGATAAAGTATGATAGGTAAAATAATAAATGAACGata < 2:254454/140‑1 (MQ=255) aaaaGAAGGTACAATAGAAGATGGAATTGATCAATTAATGCAATTGGCATTAGATAACCATTCGAAATATAACGTTACTTTCATACTCGCGGCTATTGAAGGTGATAAAGTATGATAGGTAAAATAATAAATGAACGataa > 1:267306/1‑140 (MQ=255) aaTTAAGGCAGTTGGCATTAGATAACCATTCGAAATATAACGTTACTTTCTTACTCGCGGCTATTGCATGTGACAATGTGTGATAGGTAAAATAATAAGTGAACGAGATAAAATTGTAGATCAGCCTGGCGGCGGTGGCAt < 1:202057/141‑1 (MQ=255) ggCAATTGGCATTAGATAACCATTCGAAATATAACGTTACTTTCATACTCGCGGCTATTGAAGGTGATAAAGTATGATAGGTAAAATAATAAATGAACGATATAAAATTGTAGATAAGCTTGGCGGCGGTGGCATGAGTAc < 2:204894/140‑1 (MQ=255) gCATTAGATAACCATTCGAAATATAACGTTACTTTCATACTCGCGGCTATTGAAGGGGATAAAGTATGATAGGTAAAATAATAAATGAACGATATAAAATTGTAGATAAGCTTGGCGGCGGTGGCATGAGTACCGTTTATc > 2:25811/1‑141 (MQ=255) tagtAGATAACCATTCGAAATATAACGTTACTTTCATACTCGCGGCAATTGAAGGGGATAAAGTATGATAGGTAAAATAATAAATGAACGATATAAAATTGTAGATAAGGTTGGAGAAGGTGGCATGAATACCGTTTacgt > 2:247518/4‑138 (MQ=255) aTTAGATAACCATTCGAAATATAACGTTACTTTCATACTCGCGGCTATTGAAGGTGATAAAGTATGATAGGTAAAATAATAAATGAACGATATAAAATTGTAGATAAGCTTGGCGGCGGTGGCATGAGTACCGTTTATCtt < 1:237982/141‑1 (MQ=255) ttAGATAACCATTCGAAATATAACGTTACTTTCATACTCGCGGCTATTGAAGGTGATAAAGTATGATAGGTAAAATAATAAATGAACGATATAAAATTGTAGATAAGCTTGGCGGCGGTGGCATGAGTACCGTTTATCTTg < 1:12520/141‑1 (MQ=255) cATTCGAAATATAACGTTACTTTCATACTCGCGGCTATTGAAGGTGATAAAGTATGATATGTAAAATAATAAATGAACGATATAAAATTGTAGATAAGCTTGGCGGCGGTGGCATGAGTACCGTTTATCTTGCTGAAgata > 1:205242/1‑141 (MQ=255) tCGAAATATAACGTTACTTTCATACTCGCGGCTATTGAAGGTGATAAAGTATGATAGGTAAAATAATAAATGAACGATATAAAATTGTAGATAAGCTTGGCGGCGGTGGCATGAGTACCGTTTATCTTGCTGAAgatacga < 2:178210/141‑1 (MQ=255) | ATTCAGATGGATTAACTGATTATGTTAAAGACAATGAAATTAAGCGTTTGTTAGTAAAAGAAGGTACAATAGAAGATCATGGTGATCAATTAATGCAATTGGCATTAGATAACCATTCGAAAGATAACGTTACTTTCATACTCGCGGCTATTGAAGGTGATAAAGTATGATAGGTAAAATAATAAATGAACGATATAAAATTGTAGATAAGCTTGGCGGCGGTGGCATGAGTACCGTTTATCTTGCTGAAGATACGA > CP000730/1231930‑1232186 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |