Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 1,471,910 C→T 100% E10038E (GAG→GAA)  ebh ← extracellular matrix binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007301,471,9100CT100.0% 90.3 / NA 29E10038E (GAG→GAA) ebhextracellular matrix binding protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (14/15);  total (14/15)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

CTGTTGGATTAGCTGCTTTCATTTGAGCTTTAAATTGTTCCAATTGCTCACTTATTTCATCGATGCTTTGCGCACGTTGAATCGCTTGAATTGCTTGTTCTTTTAATTGATTTAACTTAGCAATAGCTTCTTGCTTCTCTTTATCTGTTAGATCAGTACGAGCTTTGATTTCATCAATCATATGTTGAATTGCATCTTCAATCGATTTAATTGCATTTGATTTTGCTTGTTCAATCGTAAATTGTTCTGGATTAAATTGTT  >  CP000730/1471774‑1472034
                                                                                                                                        |                                                                                                                            
cTGTTGGATTAGCTGCTTTCATTTGAGCTTTAAATTGTTCCAATTGCTCACTTATTTCATCGATGCTTTGCGCACGTTGAATCGCTTGAATTGCTTGTTCTTTTAATTGATTTAACTTAGCAATAGCTTCTTGCTTTTCtt                                                                                                                          >  2:31909/1‑141 (MQ=255)
          aGCTGCTTTCATTTGAGCTTTAAATTGTTCCAATTGCTCACTTATTTCATCGATGCTTTGCGCACGTTGAATCGCTTGAATTGCTTGTTCTTTTAATTGATTTAACTTAGCAATAGCTTCTTGCTTTTCTTTATCTTTTAg                                                                                                                >  2:85786/1‑141 (MQ=255)
          aGCTGCTTTCATTTGAGCTTTAAATTGTTCCAATTGCTCACTTATTTCATCGATGCTTTGCGCACGTTGAATCGCTTGAATTGCTTGTTCTTTTAATTGATTTAACTTAGCAATAGCTTCTTGCTTTTCTTTATCTGTTAg                                                                                                                >  1:254483/1‑141 (MQ=255)
                    aTTTGAGCTTTAAATTGTTGCAATTGCTCACTTATTTCATCGATGCTTTGCGCACGTTGCATCGCTTGAATTGCTTGTTCTTTTAATTGATTTAACTTAGCAATAGCCTCTTGCTTTTCTTTATCTGTCAGATCAGTACGa                                                                                                      >  2:151182/1‑141 (MQ=255)
                    aTTTGAGCTTTAAATTGTTCCAATTGCTCACTTATTTCATCGATGCTTTGCGCACGTTGAATCGCTTGAATTGCTTGTTCTTTTAATTGATTTAACTTAGCAATAGCTTCTTGCTTTTCTTTATCTGTTAGATCAGTACGa                                                                                                      >  1:135134/1‑141 (MQ=255)
                     tttGAGCTTTAAATTGTTCCAATTGCTCACTTATTTCATCGATGCTTTGCGCACGTTGAATCGCTTGAATTGCTTGTTCTTTTAATTGATTTAACTTAGCGATAGCTTCTTGCTTTTCTTTATCTGTTAGATCAGTACGAg                                                                                                     >  1:95988/1‑141 (MQ=255)
                      ttGCGCTTTAAATTGTTCCAATTGCTCACTTATTTCATCGATGCTTTGCGCACGTTGAATCGCTTGAATTGCTTGTTCTTTTAATTGATTTAACTTAGCAATAGCTTCTTGCTTTTCTTTATCTGTTAGATCAGTACGAGc                                                                                                    <  2:18540/141‑1 (MQ=255)
                         aGCTTTAAATTGTTCCAATTGCTCACTTATTTCATCGATGCTTTGCGCACGTTGAATCGCTTGAATTGCTTGTTCTTTTAATTGATTTAACTTAGCAATAGCTTCTTGCTTTTCTTTATCTGTTAGATCAGTACGAGCttt                                                                                                 <  1:8734/141‑1 (MQ=255)
                             ttAAATTGTTCCAATTGCTCACTTATTTCATCGATGCTTTGCGCACGTTGAATCGCTTGAATTGCTTGTTCTTTTAATTGATTTAACTTAGCAATAGCTTCTTGCTTTTCTTTATCTGTTAGATCAGTACGAGCTTTGAtt                                                                                             <  1:234095/141‑1 (MQ=255)
                              tAAATTGTTCCAATTGCTCACTTATTTCATCGATGCTTTGCGCACGTTGAATCGCTTGAATTGCTTGTTCTTTTAATTGATTTAACTTAGCAATAGCTTCTTGCTTTTCTTTATCTGTTAGATCAGTACGAGCTTTGAttt                                                                                            >  2:75493/1‑141 (MQ=255)
                                 aTTGTTCCAATTGCTCACTTATTTCATCGATGCTTTGCGCACGTTGAATCGCTTGAATTGCTTGTTCTTTTAATTGATTTAACTTAGCAATAGCTTCTTGCTTTTCTTTATCTGTTAGATCAGTACGAGCTTTGATTtcat                                                                                         <  1:81937/141‑1 (MQ=255)
                                                    tATTTCATCGATGCTTTGCGCACGTTGAATCGCTTGAATTGCTTGTTCTTTTAATTGATTTAACTTAGCAATAGCTTCTTGCTTTTCTTTATCTGTTAGATCAGTACGAGCTTTGATTTCATCAATCATATGTTGAATTGc                                                                      <  1:207309/141‑1 (MQ=255)
                                                     aTTTCATCGATGCTTTGCGCACGTTGAATCGCTTGAATTGCTTGTTCTTTTAATTGATTTAACTTAGCAATAGCTTCTTGCTTTTCTTTATCTGTTAGATCAGTACGAGCTTTGCTTTCATCAATCATATGTTGAATTGcc                                                                     >  2:5948/1‑140 (MQ=255)
                                                        tCATCGATGCTTTGCGCACGTTGAATCGCTTGAATTGCTTGTTCTTTTAATTGATTTAACTTAGCAATAGCTTCTTGCTTTTCTTTATCTGTTAGATCAGTACGAGCTTTGATTTCATCAATCATATGTTGAATTGCATCt                                                                  >  1:240332/1‑141 (MQ=255)
                                                              gtGCTTTTCGCACTTTGAATCGCTTGAATTGCTTGTTCTTTTAATTGATTTAACTTAGCAATAGCTTCTTGCTTTTCTTTATCTGTTAGATCAGTACGAGCTTTGATTTCATCAATCATATGTTGAATTGCATCTTCAATc                                                            <  2:129164/140‑1 (MQ=255)
                                                                    tGCGCACGTTGAATCGCTTGAATTGCTTGTTCTTTTAATTGATTTAACTTAGCAATAGCTTCTTGCTTTTCTTTATCTGTTAGATCAGTACAAGATTTGATTTCATCAATAATATGTTGAATTACAGCTGCAGTCGATTTa                                                      >  2:254044/1‑141 (MQ=255)
                                                                      cgcACGTTGAATCGCTTGAATTGCTTGTTCTTTTAATTGATTTAACTTAGCAATAGCTTCTTGCTTTTCTTTATCTGTTAGATCAGTACGAGCTTTGATTTCATCAATCATATGTTGAATTGCATCTTCAATCGATTTAAt                                                    >  1:222556/1‑141 (MQ=255)
                                                                         tcGTTGAATCGCTTGAATTGCTTGTTCTTTTAATTGATTTAACTTAGCAATAGGTTCTTGCTTTTCTTTATCTGTTAGATCAGTACGAGCTTTGATTTCATCAATCATATGTTGAATTGCATCTTCAATCGATTTAATTGc                                                 <  2:148443/140‑1 (MQ=255)
                                                                            ttttaTCGCTTTAATTGCTTGTTCTTTTAATTGATTTAACTGTGCAATAGCTTCTTGCTTTTCTTTATCTGTTAGATCAGTACGATCTTTGATTTCATCAATCATATGGTGACTTGCATCTTCAATCGATTTAATTGCAtt                                              <  2:93019/137‑1 (MQ=255)
                                                                            ttGAATCGCTTTAATTTCTTGTTCTTTTAATTTATTTAACTTAGCAATAGCTTCTTGCTTTTCTTTATCTGTTAGATCAGTACGAGCTTTGATTTCATCAATCATATGTTGAATTGCATCTTCAATCGATTTAATTGCAtt                                              <  2:117108/141‑1 (MQ=255)
                                                                                aTCGCTTGAATTGCTTGTTCTTTTAATTGATTTAACTTAGCAATAGCTTCTTGCTTTTCTTTATCTGTTAGATCAGTACGAGCTTTGATTTCATCAATCATATGTTGAATTGCATCTTCAATCGATTTAATTGCATTTGAt                                          >  2:199348/1‑141 (MQ=255)
                                                                                 tCGCTTGAATTGCTTGTTCTTTTAATTGATTTAACTTAGCAATAGCTTCTTGCTTTTCTTTATCTGTTAGATCAGTACGAGCTTTGATTTCATCAATCATATGTTGAATTGCATCTTCAACCGATTTAATTGCATTTGAtt                                         <  1:199348/141‑1 (MQ=255)
                                                                                  cGCTTGAATTGCTTGTTCTTTTAATTGATTTAACTTAGCAATAGCTTCTTGCTTTTCTTTATCTGTTAGATCAGTACGAGCTTTGATTTCATCAATCATATGTTGAATTGCATCTTCAATCGATTTAATTGCATTTGAttt                                        <  1:82507/141‑1 (MQ=255)
                                                                                      tGAATTGCTTGTTCTTTTAATTGATTTAACTTCGCAATAGCTTCTTGCTTTTCTTTATCTGTTAGACCAGTACGAGCTTTGATTTCATCAATCATATGTTGAATTGCATCTTCAATCGATTTAATTGCATTTGATTTTGCt                                    <  1:90620/141‑1 (MQ=255)
                                                                                                         aTTGATTTAACTTAGCAATAACTTCTTGCTTTTCTTTATCTGTTAGATCAGTACGAGCTTTGATTTCATCAATCATATGTTGAATTGCATCTTCAATCGATTTAATTGCATTTGATTTTGCTTGTTCAATCGTAAATTGtt                 >  1:190042/1‑141 (MQ=255)
                                                                                                          ttGATTTAACTTAGCAATAGCTTCTTGCTTTTCTTTATCTGTTAGATCAGTACGAGCTTTGATTTCATCAATCATATGTTGAATTGCATCTTCAATCGATTTAATTGCATTTGATTTTGCTTGTTCAATCGTAAATTGTTc                <  2:222556/141‑1 (MQ=255)
                                                                                                                tAACTTAGCAATAGCTTCTTGCTTTTCTTTATCTGTTAGATCAGTACGAGCTTTGATTTCATCAATCATATGTTGAATTGCATCTTCAATCGATTTAATTGCATTTGATTTTGCTTGTTCAATCGTAAATTGTTCTGGAtt          <  2:254483/141‑1 (MQ=255)
                                                                                                                   cTTAGCAATAGCTTCTTGCTTTTCTTTATCTGTTAGATCAGTACGAGCTTTGATTTCATCAATCATATGTTGAATTGCATCTTCAATCGATTTAATTGCATTTGATTTTGCTTGTTCAATCGTAAATTGTTCTGGATTaaa       <  2:16466/141‑1 (MQ=255)
                                                                                                                        cAATAGCTATTTCCTTTTCTTTATCTGTTCGATAATGGCGAGCTTTGCTTTGATACATCTAATGTTGTGGTGCATCTTCAATCGATTTAATTGCTTTTGATTTTGCTTGTTCAATCGTGAATTGTTCTGGTTTAAATTGtt  >  2:56729/1‑141 (MQ=255)
                                                                                                                                        |                                                                                                                            
CTGTTGGATTAGCTGCTTTCATTTGAGCTTTAAATTGTTCCAATTGCTCACTTATTTCATCGATGCTTTGCGCACGTTGAATCGCTTGAATTGCTTGTTCTTTTAATTGATTTAACTTAGCAATAGCTTCTTGCTTCTCTTTATCTGTTAGATCAGTACGAGCTTTGATTTCATCAATCATATGTTGAATTGCATCTTCAATCGATTTAATTGCATTTGATTTTGCTTGTTCAATCGTAAATTGTTCTGGATTAAATTGTT  >  CP000730/1471774‑1472034

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑