Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 1,539,250 C→G 100% intergenic (‑349/+37) aroC ← / ← USA300HOU_1405 chorismate synthase/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007301,539,2500CG100.0% 50.9 / ‑6.5 21intergenic (‑349/+37)aroC/USA300HOU_1405chorismate synthase/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base G (12/8);  minor base A (1/0);  total (13/8)
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

AATGATTAGCCGTTTTAATATAATCGTATCGCAACTTTAGCAAATAATATACTGACTACATTTCTTAATACAAAAGAACGAACTGTTAATTATTAATAAAGTTAATCAGTCCGTTTAAAAAAATTATGATATTAAAATATACATATGTTAAGCGCTTTTGTTATACTTTGACTTCAAAAATATAATCAATTCTTTTACTAATACATAAAAAATATAAATGAGAGTCAGTATAAAAATAATTTTTG  >  CP000730/1539140‑1539384
                                                                                                              |                                                                                                                                      
aaTGATTAGCCGTTTTAATATAATCGTATCGCAACTTTAGCAAATAATATACTGACTACATTTCTTAATACAAAAGAACGAACTGTTAATTATTAATAAAGTTAATCAGTACGTTTAAAAAAATTATGATATTAAAAtata                                                                                                          >  2:111652/1‑141 (MQ=255)
  tGATTAGCCGTTTTAATATAATCGTATCGCAACTTTAGCAAATAATATACTGACTACATTTCTTAATACAAAAGAACGAACTGTTAATTATTAATAAAGTTAATCAGTGCGTTTAAAAAAATTATGATATTAAAATATACa                                                                                                        >  1:225595/1‑141 (MQ=255)
   gATTAGCCGTTTTAATATAATCGGATCGCAACTTTAGCAAATAATATACTGACTACATTTCTTAATACTAAAGATCGAACTGTTAATTATTAATAAAGTTAATCAGTGCGTTTAAAAAAATTATGATATTAAAATATACat                                                                                                       <  2:14750/141‑1 (MQ=255)
             tttAATATAATCGTATCGCAACTTTAGCAAATAATATACTGACTACATTTCTTAATACAAAAGAACGAACTGTTAATTATTAATAAAAGAAAACAGTGCGTTTAAAAAAATTATGATATTAAAATATACATATGTTAAgcg                                                                                             >  1:117377/1‑141 (MQ=255)
                 atatAATCGTATCGCAACTTTAGCAAATAATATACTGACTACATTTCTTAATACAAAAGAACGAACTGTTAATTATTAATAAAGTTAATCAGTGCGTTTAAAAAAATTATGATATTAAAATATCCATATTCTAAGCGAttt                                                                                         >  1:101037/1‑141 (MQ=255)
                     aaTCGTATCGCAACTTTAGCAAATAATATACTGACTACATTTCTTAATACAAAAGAACGAACTGTTAATTATTAATAAAGTTAATCAGTGCGTTTAAAAAAATTATGATATTAAAATATACATATGTTAAGCGCTTTTGtt                                                                                     <  2:150969/141‑1 (MQ=255)
                          tATCGCAACTTAAACAAATAATACACTAACTACATACCTTAATACAAAAGAACCAACCGTGAATTATGAATAAACGTAATCATTGCGTTTAAAAAAATTATGATATTAAAATATACATATGTTAAGCGCTTTTGTTATACt                                                                                <  2:23305/141‑1 (MQ=255)
                           aTCGCAACTTTAGCAAATAATATACTGACTACATTTCTTAATACAAAAGAACGAACTGTTAATTATTAATAAAGTTAATCAGTGCGTTTAAAAAAATTATGATATTAAAATATACATATGTTAAGCGCTTTTGTTATACtt                                                                               >  2:214030/1‑141 (MQ=255)
                                aacataaGCAAATAATATACTGACTACATTTCTTAATACAAAAGAACGAACTGTTAATTATTAATAAAGTTAATCAAAGAAAAAAAAAAAATTATGATATTAAAATATACATATGTTAAGCGCTTTTGTTATACTTTGACt                                                                          <  2:85642/135‑1 (MQ=255)
                                       gCAAATAATATACTGACTACATTTCTTAATACAAAAGAACGAACTGTTAATTATTAATAAAGTTAATCAGTGCGTTTAAAAAAATTATGATATTAAAATATACATATGTTAAGCGCTTTTGTTATACTTTGACTTCaaaaa                                                                   <  1:226519/141‑1 (MQ=255)
                                        cAAATAATATACTGACTACATTTCTTAATACAAAAGAACGAACTGTTAATTATTAATAAAGTTAATCAGTGCGTTTAAAAAAATTATGATATTAAAATATACATATGTTAAGCGCTTTTGTTATACTTTGACTTCAAAAat                                                                  >  2:5103/1‑141 (MQ=255)
                                                                  caTACAAAAGAACGAACTGTTAATTATTAATAAAGTTAATCAGTGCGTTTAAAAAAATTATGATATTAAAATATACATATGTTAAGCGCTTTTGTTATACTTTGACTTCAAAAATATATTCAATTCTTTAACTACTCCATa                                        >  2:27476/2‑141 (MQ=255)
                                                                  aaTACAAAAGAACGAACTGTTAATTATTAATAAAGTTAATCAGTGCGTTTAAAAAAATTATGATATTAAAATATACATATGTTAAGCGCTTTTGTTATACTTTGACTTCAAAAATATAATCAATTCTTTTACTAATACATa                                        >  2:243288/1‑141 (MQ=255)
                                                                   aTACAAAAGAACGAACTGTTAATTATTAATAAAGTTAATCAGTGCGTTTACAAAAATTATGTTATTAATATATCCATATGTTAAGCGCTTTTGTTATACTTTGACTTCAAAAATATAATCAATTCTTTTACTAATACATaa                                       >  2:52755/1‑141 (MQ=255)
                                                                             acgaacTGTTAATTATTAATAAAGTTAATCAGTGCGTTTAAAAAAATTATGATATTAAAATATACATATGTTAAGCGCTTTTGTTATACTTTGACTTCAAAAATATAATCAATTCTTTTACTAATACATAAAAAATATaaa                             >  1:149914/1‑141 (MQ=255)
                                                                              cgaacTGTAAATTATTAATAAAATTAATCAGTGAGTTTAAAAAAATTATGATATTAAAATATACATATGTTAAGCGCTTTTGTTATACTTTGACTTCAAAAATATAATCAATTCTTTTACTAATACATAAAAAATATAAAt                            <  2:87200/141‑1 (MQ=255)
                                                                               gaacTGTTAATTATTAATAAAGTTGATCAGTGCGTTTAAAAAAATTATGATATTAAAATATACATATGTTAAGCGCTTTTGTTATACTTTGACTTCAAAAATATAATCAATTCTTTTACTAATACATAAAAAATATAAATg                           >  1:197439/1‑141 (MQ=255)
                                                                                        attattAATAAAGTTAATCAGTGCGTTTAAAAAAATTATGATATTAAAATATACATATGTTAAGCGCTTTGGTTATACTTTGACTTCAAAAATATAATCAATTCTTTTACTAATACATAAAAAATATAAATGAGAGTCAGt                  >  1:180206/1‑141 (MQ=255)
                                                                                              aataaAGTTAATCAGTGCGTTTAAAAAAATTATGATATTAAAATATACATATGTTAAGCGCTTTTGTTATACTTTGACTTCAAAAATATAATCAATTCTTTTACTAATACATAAAAAATATAAATGAGAGTCAGTATaaaa            <  1:106249/141‑1 (MQ=255)
                                                                                                   aGTTAATCAGTGCGTTTAAAAAAATTATGATAATAAAATATACATATGTTAAGCGCTTTTGTTATACTTTGACTTCAAAAATATAATCAATTCTTTTACTAATACATAAAAAATATAAATGAGAGTCAGTATAAAaataat       <  1:60449/141‑1 (MQ=255)
                                                                                                        aTCAGTGCGTTTAAAAAAATTATGATATTAAAATATACATATGTTAAGCGCTTTTGTTATACTTTGACTTCAAAAATATAATCAATTCTTTTACTAATACATAAAAAATATAAATGAGAGTCAGTATAAAAATAATTTTTg  >  2:237695/1‑141 (MQ=255)
                                                                                                              |                                                                                                                                      
AATGATTAGCCGTTTTAATATAATCGTATCGCAACTTTAGCAAATAATATACTGACTACATTTCTTAATACAAAAGAACGAACTGTTAATTATTAATAAAGTTAATCAGTCCGTTTAAAAAAATTATGATATTAAAATATACATATGTTAAGCGCTTTTGTTATACTTTGACTTCAAAAATATAATCAATTCTTTTACTAATACATAAAAAATATAAATGAGAGTCAGTATAAAAATAATTTTTG  >  CP000730/1539140‑1539384

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: