| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP000730 | 1,672,590 | G→T | 100% | L135I (CTT→ATT) | rpoD ← | DNA‑directed RNA polymerase sigma subunit RpoD |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP000730 | 1,672,590 | 0 | G | T | 95.9% | 60.7 / ‑4.4 | 24 | L135I (CTT→ATT) | rpoD | DNA‑directed RNA polymerase sigma subunit RpoD |
| Reads supporting (aligned to +/- strand): ref base G (1/0); new base T (8/15); total (9/15) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.75e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.46e-01 | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
TCCTTTGTTAAAGTCAAATTTTTCAACAGCTTTAATAAGACCCATATTACCTTCTTGGATTAAATCAAGGAATAACATACCACGACCTACGTATCTTTTAGCAATACTTACAACTAAACGTAAGTTCGCTTCTGCAAGTCTTGATTTTGCTACTTCATCACCTTGTTCAATACGTTTGGCTAATTCGATTTCTTCTTGTGCACTTAATAAGTTAACACGCCCAATTTCTTTAAGGTACATACGAACTGGGTCATTTATTTTAACACCTGGA > CP000730/1672453‑1672723 | tCCTTTGTTAAAGTCAAATTTTTCAACAGCTTTAATAAGACCCATATTACCTTCTTGGATTAAATCAAGGAATAACATACCACGACCTACGTATCTTTTAGCAATACTTACAACTAAACGTAAGTTCGCTTCTGCAATTCt > 1:262172/1‑141 (MQ=255) gTTAAAGTCAAATTTTTCAACAGCTTTAATAAGACCCATATTACCTTCTTGGATTAAATCAAGGAATAACATACCACGACCTACGTATCTTTTAGCAATACTTACAACTAATTGGAAGTTCGCTTCTGCAATTCTTGAttt < 1:131032/141‑1 (MQ=255) cAAATTTTTCAACAGCTTTAATAAGACCCATATTACCTTCTTGGATTAAATCAAGGAATAACATACCACGACCTACGTATCTTTTAGCAATACTTACAACTAAACGTAAGTTCGCTTCTGCAATTCTTGATTTTGCTACtt < 2:59746/141‑1 (MQ=255) cAAATTTTTCAACAGCTTTAATAAGACCCATATTACCTTCTTGGATTAAATCAAGGAATAACATACCACGACCTACGTATCTTTTAGCAATACTTACAACTAAACGTAAGTTCGCTTCTGCAATTCTTGATTTTGCTACtt < 2:254513/141‑1 (MQ=255) aTTTTTCAACAGCTTTAATAAGACCCATATTACCTTCTTGGATTAAATCAAGGCATCTCATACCACGACCTACGTATCTTTTAGCAATACTTACAACTAAACGTAAGTTCGCTTCTGCAATTCTTGATTTTGCTACTtcat < 1:61997/141‑1 (MQ=255) tCAACAGCTTTAATAAGACCCATATTACCTTCTTGGATTAAATCAAGGAATAACATACCACGACCTACGTATCTTTTAGCAATACTTACAACTAAACGTAAGTTCGCTTCTGCAATTCTTGACTTTGCTACTTCACCACCt > 2:27585/1‑141 (MQ=255) cTTTAATAAGACCCATATTACCTTCTTGGATTAAATCAAGGAATAACATACCACGACCTACGTATCTTTTAGCAATACTTACAACTAAACGTAAGTTCGCTTCTGCAATTCTTGATTTTGCTACTTCATCACCTTGTTCaa < 2:101331/141‑1 (MQ=255) aataaGACCCATATTACCTTCTTGGATTAAATCAAGGAATAACATACCACGACCTACGTATCTTTTAGCAATACTTACAACTAAACGTAAGTTCGCTTCTGCAATTCTTGATTTTGCTACTTCATCACCTTGTTCAATACg > 1:179572/1‑141 (MQ=255) atTACCTTCTTGGATTAAATCAAGGAATAACATACCACGACCTACGTATCTTTTAGCAATACTTACAACTAAACGTAAGTTCGCTTCTGCAATTCTTGATTTTGCTACTTCATCACCTTGTTCAATACGTTTGGCTAATTc > 2:34379/1‑141 (MQ=255) gATTAAATCAAGGAATAACATACCACGACCTACGTATCTTTTAGCAATACTTACAACTAAACGTAAGTTCGCTTCTGCAATTCTTGATTTTGCTACTTCATCACCTTGTTCAATACGTTTGGCTAATTCGATTTCTTCTtg < 1:55365/141‑1 (MQ=255) aTCAAGGAATAACCTGCCACGACCTGCGTATCTTTTAGCAATACTTTCAACTTAACGTAAGTTCGCTTCTGCAATTCTTGTTGTTGCTACTTCAGCACCTTGTTCAATACGTTTTGCTAATTCGATTTCTTCTTGTGCGCt < 2:81195/141‑1 (MQ=255) aTCAAGGAATAACATACCACGACCTACGTATCTTTTAGCAATACTTCCAACTAAACGTAAGTTCGCTTCTGCAAGTCTTGATTTTGCTACTTCATCACCTTGTTCAATACGTTTGGTTATTCCGATTTCTTCTTGTGCACt > 1:124773/1‑141 (MQ=255) tACCACGCCCTACGTATCTTTTAGCAATACTTACAACTAAACGTAAGTTCGCTTCTGCAATTCTTGATTTTGCTACTTCATCCCCTTGTTCAATACGTTTGGCTAATTCGATTTCTTCTTGTGCACTTAATAAGTTAacac < 2:166538/141‑1 (MQ=255) aCCACGACCTACGTATCTTTTAGCAATACTTACAACTAAACGTAAGTTCGCTTCTGCAATTCTTGATTTTGCTACTTCATCACCTTGTTCAATACGTTTGGCTAATTCGATTTCTTCTTGTGCACTTAATAAGTTAACACg < 2:255228/141‑1 (MQ=255) ccACGACCTACGTATCTTTTAGCAATACTTACAACTAAACGTAAGTTCGCTTCTGCAATTCTTGATTTTGCTACTTCATCACCTTGTTCAATACGTTTGGCTAATTCGATTTCTTCTTGTGCACTTAATAAGTTAACACGc < 1:152948/141‑1 (MQ=255) aCCTACGTATCTTTTAGCAATACTTACAACTAAACGTAAGTTCGCTTCTGCAATTCTTGATTTTGCTACTTCATCACCTTGTTCAATACGTTTGGCTAATTCGATTTCTTCTTGTGCACTTAATAAGTTAACACGCCCAAt > 2:210385/1‑141 (MQ=255) aCGTATCTTTTAGCAATACTTACGACTAAACGTAAGTTCGCTTCTGCAATTCTTGATTTTGCTACTTCATCACCTTGTTCCATACGTTTGGCTAATTCGATTTCTTCTTGTGCACTTAATAAGTTAACACGCCCAATTTCt > 2:193700/1‑141 (MQ=255) aCGTATCTTTTAGCAATACTTACAACTAAACGTAAGTTCGCTTCTGCAATTCTTGATTTTGCTACTTCATCACCTTGTTCAATACGTTTGGCTAATTCGATTTCTTCTTGTGCACTTAATAAGTTAACACGCCCAATTTCt < 1:262224/141‑1 (MQ=255) ttttAGCAATACTTACAACTAAACGTAAGTTCGCTTCTGCAATTCTTGATTTTGCTACTTCATCACCTTGTTCAATACGTTTGGCTAATTCGATTTCTTCTTGTGCACTTAATAAGTTAACACGCCCAATTTCTTTAAGGt > 1:267618/1‑141 (MQ=255) aGCAATACTTACAACTAAACGTAAGTTCGCTTCTGCAATTCTTAATTTTGCTACTTCATCACCTTGTTCAATACGTTTGGCTAATTCGATTTCTTCTTGTGCACTTAATAAGTTAACACACCCATTTTCTTTAAGGTAAAt > 1:204995/1‑141 (MQ=255) cTTACAACTAAACGGAAGTTCGCTTCTGCAATTCTTGATTTTGCTACTTCATCACCTTGTTCAATACGTTTGGCTAATTCGATTTCTTCTTGTGCACTTAATAAGTTAACACGCCCAATTTCTTTAAGGTACATACGAACt < 1:95148/141‑1 (MQ=255) cTTACAACGAAACGTAAGTTCGCTTCTGCAATTCTTGATTTTGCTACTTCATCACCTTGTTCAATACGTTTGGCTAATTCGATTTCTTCTTGTGCACTTAATAAGTTAACACGCCCAATTTCTTTAAGGTACATACGAACt < 1:39046/141‑1 (MQ=255) cgtgtGCAATTCTTGATTTTGCTACTTCATCACCTTGTTCAATACGTTTGGCTAATTCGATTTCTTCTTGTGCACTTAATAAGTTAACACGCCCAATTTCTTTAAGGTACATACGAACTGGGTCATTTATTTTAACACCTg < 2:156797/137‑1 (MQ=255) tCTGCAATTCTTGATTTTGCTACTTCATCACCTTGTTCAATTCGTTTGGCTAATTCGATTTCTTCTTGTGCACTTAATAAGTTAACACGCCCAATTTCTTTAAGGTACATACGAACTGGGTCATTTATTTTAACACCTGGa < 1:34379/141‑1 (MQ=255) | TCCTTTGTTAAAGTCAAATTTTTCAACAGCTTTAATAAGACCCATATTACCTTCTTGGATTAAATCAAGGAATAACATACCACGACCTACGTATCTTTTAGCAATACTTACAACTAAACGTAAGTTCGCTTCTGCAAGTCTTGATTTTGCTACTTCATCACCTTGTTCAATACGTTTGGCTAATTCGATTTCTTCTTGTGCACTTAATAAGTTAACACGCCCAATTTCTTTAAGGTACATACGAACTGGGTCATTTATTTTAACACCTGGA > CP000730/1672453‑1672723 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |