Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 36,015 C→T 28.3% R179R (AGG→AGA USA300HOU_0026 ← IS431mec transposase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP00073036,0150CT28.3% 1318.4 / inf 914R179R (AGG→AGAUSA300HOU_0026IS431mec transposase
Reads supporting (aligned to +/- strand):  ref base C (323/327);  new base T (149/110);  total (475/439)
Fisher's exact test for biased strand distribution p-value = 3.33e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.92e-01

Only 100 of 938 total aligned reads displayed.

GCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAGCCATTGCTACCTTCGTTGAAGGTGCCTGATC  >  CP000730/35875‑36155
                                                                                                                                            |                                                                                                                                            
 tttAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATAcc                                                                                                                                             <  1:806536/140‑1 (MQ=17)
 cTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATAcc                                                                                                                                             >  1:412363/1‑141 (MQ=17)
 cTTAACTTGCTAGCATGATGCCAATTTCGTGGCATGGCGATAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATAcc                                                                                                                                             <  1:78287/141‑1 (MQ=12)
    aaCTTGCTAGCATGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTg                                                                                                                                          >  1:617056/1‑141 (MQ=11)
      cTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATCCCTTTTAAAGTATTCTTTGCTGGATTGATACTTTGATACATTGtt                                                                                                                                        >  1:89962/1‑140 (MQ=255)
       ttGCTAGCATGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGtct                                                                                                                                       <  1:727610/141‑1 (MQ=11)
        tGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGtctt                                                                                                                                      <  1:151694/141‑1 (MQ=17)
          cTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGtctttc                                                                                                                                    >  2:783056/1‑141 (MQ=17)
           tAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGtctttct                                                                                                                                   >  2:515313/1‑141 (MQ=32)
                 gatgCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACttt                                                                                                                             <  1:63967/141‑1 (MQ=25)
                  atgCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATGCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTa                                                                                                                            >  1:190544/1‑141 (MQ=255)
                    tcTAATTTCGTGGCATGGCGAAAATCCGTGGATCTTAAGTGTCCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTATAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAat                                                                                                                          <  2:91972/140‑1 (MQ=11)
                       taTTTCGTGGCTTGGCGAAAATCCGTGGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATg                                                                                                                       <  1:205320/140‑1 (MQ=255)
                       actttCGTGGCATGGCGAAATTCCGTAGATCTGAAGGGACCTGCGGTTCTTTTTATATAGGGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATg                                                                                                                       <  2:68008/139‑1 (MQ=255)
                         tttCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGAc                                                                                                                     <  1:699170/141‑1 (MQ=25)
                            cGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGATCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGTTATCTTGTCTTTCTTACTTTAATATGCCggg                                                                                                                  >  2:146515/1‑140 (MQ=11)
                             gTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTg                                                                                                                 <  2:162454/141‑1 (MQ=255)
                             gTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTg                                                                                                                 <  1:580721/141‑1 (MQ=255)
                             gTGGAATGGCGAAAATCCGGAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTg                                                                                                                 <  1:66662/141‑1 (MQ=255)
                              tGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGa                                                                                                                >  1:882346/1‑141 (MQ=37)
                                gCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAGAGTATTCTTGGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTGAATATGACGGGGAtt                                                                                                              >  1:73498/1‑140 (MQ=11)
                                gCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATc                                                                                                              <  1:534863/141‑1 (MQ=255)
                                 cATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCt                                                                                                             >  1:113550/1‑141 (MQ=37)
                                  aTGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCtt                                                                                                            >  2:404286/1‑141 (MQ=37)
                                   tGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTg                                                                                                           <  2:366895/141‑1 (MQ=255)
                                       gAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCa                                                                                                       >  1:299200/1‑141 (MQ=38)
                                       gAAAACCCGTAGATCTGAACAGACTTGCGGTTCTTTTTCTATAGCGCCTAAATACACTCAATACCTTTTTATGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCa                                                                                                       <  1:255610/141‑1 (MQ=11)
                                         aaaTCCATAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAAt                                                                                                     >  2:572165/1‑141 (MQ=255)
                                           aTCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGa                                                                                                   <  1:144333/141‑1 (MQ=38)
                                             ccGTAGATCTGAAGAGATCTTCTGTTGTGTTTGTATAGAGCTTAAATACATTCAATACCTTTTAAAGTACTCTTTTCCGTATGGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAgt                                                                                                 <  1:17836/141‑2 (MQ=11)
                                               gTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGtt                                                                                               >  2:381893/1‑141 (MQ=38)
                                               gTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGtt                                                                                               >  2:613082/1‑141 (MQ=38)
                                               gTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGtt                                                                                               >  1:468502/1‑141 (MQ=255)
                                                tAGATCTGGAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTTCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGttt                                                                                              >  1:101997/1‑140 (MQ=11)
                                                tAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGAGCTTGCTCAATGAGGTTa                                                                                              >  1:689689/1‑141 (MQ=255)
                                                  gATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTAtt                                                                                            <  1:266036/141‑1 (MQ=255)
                                                  gATCTGAAGAGACCTACGGTTCTTTTTATATAGTGCGTACCTCTTTTGAATTACTTTTCAAGGAATCTTTGCCGTATTGATACTTTGAGATCGTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTAtt                                                                                            <  2:877330/141‑1 (MQ=14)
                                                     cTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCATGCTCAATGAGGTTATTCAg                                                                                         <  1:551881/141‑1 (MQ=255)
                                                      ttaaGAGACCTGCGGTTCTTTTTATAGAGAGCGTAAATACCCTCAATACCTTTTAAAGTAGTCTTTGCTGTATTGCTCCTATGATCACTTGTCTTGCTAGCTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGa                                                                                        <  2:102405/139‑1 (MQ=11)
                                                      taaaGAGCTCTACGGTTCTTCTTATATACAGTTTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGa                                                                                        <  2:753593/139‑1 (MQ=11)
                                                      tGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGa                                                                                        >  1:403619/1‑141 (MQ=38)
                                                        aaGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATACTTTGCTGTATTGTTACTTTGATACATTGTCTTTCTTACTTAAATATGACAGTAATGTTGCTCAATGAGGTTCTTCAGata                                                                                      >  2:501568/1‑141 (MQ=11)
                                                             aCCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCg                                                                                 <  1:284492/141‑1 (MQ=255)
                                                               ctacGGTTCTTTTTATATAGAGCTTAAATACATTCAATACCTTTTAAAGTATTCTTTCCCGTATTGATACTTTCATATCTTGTCTTTCTTACTTTAATATTACGTTGATCTTGCTCAATGAGTTTATTCAAATATTTCTAt                                                                               <  2:244192/138‑1 (MQ=11)
                                                                 tcGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGt                                                                             >  2:336358/2‑141 (MQ=255)
                                                                       cTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATg                                                                       <  1:309613/141‑1 (MQ=255)
                                                                         ttttATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATTCTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGAc                                                                     <  2:811027/141‑1 (MQ=255)
                                                                          tttATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCa                                                                    >  1:338402/1‑141 (MQ=255)
                                                                          tttATATAGAGCGTAAATACACTCAATACCTTTTAAAGTAATCTTTTCTGGATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCa                                                                    >  1:861372/1‑141 (MQ=14)
                                                                           ttATATTGGGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAg                                                                   <  2:894266/141‑1 (MQ=255)
                                                                            tatataGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGt                                                                  <  2:415588/141‑1 (MQ=255)
                                                                            tatataGAGCGGAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGt                                                                  <  1:328576/141‑1 (MQ=255)
                                                                             atataGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTc                                                                 <  2:48630/141‑1 (MQ=255)
                                                                                  gagCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGgtt                                                            >  2:820210/1‑141 (MQ=255)
                                                                                   agCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATCCTTTGACATCTTGTCTTTCTTACTTTAATATGACGTTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGgttt                                                           >  1:484309/1‑141 (MQ=14)
                                                                                    gCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGgttta                                                          <  1:519521/141‑1 (MQ=255)
                                                                                      gTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGgtttaag                                                        <  1:374483/141‑1 (MQ=255)
                                                                                      gTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGgtttaag                                                        >  2:663146/1‑141 (MQ=255)
                                                                                      gTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGgtttaag                                                        <  1:761260/141‑1 (MQ=255)
                                                                                      gTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGgtttaag                                                        <  2:722473/141‑1 (MQ=255)
                                                                                      gTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGgtttaag                                                        <  1:828706/141‑1 (MQ=255)
                                                                                       tAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATATGATGCAATGTGGTTATTCAGATATTTCGATGTACAATGACAGTCAGgtttaagt                                                       >  1:855879/1‑141 (MQ=14)
                                                                                       tAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGgtttaagt                                                       <  2:865162/141‑1 (MQ=255)
                                                                                       tAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGgtttaagt                                                       >  1:631602/1‑141 (MQ=255)
                                                                                       tAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGgtttaagt                                                       <  2:537464/141‑1 (MQ=255)
                                                                                        aaaTACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTTAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGgtttaagtt                                                      <  2:765738/141‑1 (MQ=255)
                                                                                        aaaTACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGCTGTACAATGACAGTCGGgtttaagtt                                                      >  2:186508/1‑141 (MQ=255)
                                                                                        aaaTACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGgtttaagtt                                                      >  2:228843/1‑141 (MQ=255)
                                                                                         aaTACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGgtttaagttt                                                     >  2:730692/1‑141 (MQ=255)
                                                                                          aTACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTTCTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTAGAATGACAGTCAGgtttaagtttt                                                    >  2:119179/1‑140 (MQ=255)
                                                                                          aTACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGgtttaagttta                                                    >  2:631769/1‑141 (MQ=255)
                                                                                          aTACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGgtttaagttta                                                    >  2:455876/1‑141 (MQ=255)
                                                                                           tACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGgtttaagtttaa                                                   >  2:279768/1‑141 (MQ=255)
                                                                                            acacTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTaaa                                                  <  1:383135/141‑1 (MQ=255)
                                                                                             cactCAATACCTTTGAAAGGATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTaaaa                                                 >  1:500886/4‑141 (MQ=255)
                                                                                             cATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTAGTCTTTCTTACTTTAATATGACGGTGATCTAGCTAAATGAGGTTATTCAGATATTTCGATGTACAATGAAAGACAGAATTAAGGTTcaaa                                                 >  2:428741/1‑141 (MQ=14)
                                                                                              aTTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAg                                                <  1:747938/141‑1 (MQ=40)
                                                                                                tCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCt                                              >  1:607091/1‑141 (MQ=255)
                                                                                                     aCCTTTTAAAGTATTATTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGGTTTAAt                                         >  2:838009/1‑141 (MQ=255)
                                                                                                      ccTTTTAAAGTATTCAATGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAAtt                                        >  1:454091/1‑141 (MQ=255)
                                                                                                       cTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTa                                       >  2:802603/1‑141 (MQ=255)
                                                                                                       cTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACCGTCAGGTTTAAGTTTAAAAGCTTTAATTa                                       >  2:316921/1‑141 (MQ=255)
                                                                                                        ttttAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTAc                                      <  2:201954/141‑1 (MQ=255)
                                                                                                         tttAAAGTATTCTTTGCTGCATTGGTACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTACt                                     >  2:648268/1‑141 (MQ=255)
                                                                                                           tAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACttt                                   <  1:240831/141‑1 (MQ=255)
                                                                                                             aaGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAg                                 <  2:549543/141‑1 (MQ=40)
                                                                                                             aaGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGAGATATTACTGAATTAGGTTGTTCTGATATTTAGATGTACAATGGCCGTCAGGTTTAAGTTTAAAAGCTTTTAATACTTTaa                                 >  2:324937/1‑140 (MQ=14)
                                                                                                               gTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGTTCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAAcc                               >  2:258415/1‑141 (MQ=255)
                                                                                                                tATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGGGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCa                              >  2:358445/1‑141 (MQ=255)
                                                                                                                 aTTCTTTACCGTATTGATACGTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTAAATTATTTTAGCCAt                             >  1:568773/1‑141 (MQ=14)
                                                                                                                  ttCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATTAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCAtt                            <  2:855782/141‑1 (MQ=255)
                                                                                                                    cTTTGCTGTATTGATACTTTGATTCCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGc                          <  2:702457/141‑1 (MQ=255)
                                                                                                                    cTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACGATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGc                          <  1:35725/141‑1 (MQ=255)
                                                                                                                      ttGCCGTATTGATACTTTGATGTCTTGTATTTCTTACTTTAATATGACGGTGAACTTGCTCAATGAGGTTATTCCGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCtg                        >  2:59333/1‑140 (MQ=14)
                                                                                                                       tgccgTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCtgc                       >  2:744205/5‑139 (MQ=255)
                                                                                                                                 aTACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCTGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTCCTACCTTCGTTGag             >  1:226471/1‑140 (MQ=255)
                                                                                                                                  tACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAg            <  1:596604/141‑1 (MQ=255)
                                                                                                                                    cTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAGCCATTGCTACCTTCGTTGAAGGt          >  1:443033/1‑141 (MQ=39)
                                                                                                                                      ttGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAGCCATTGCTACCTTCGTTGAAGGTGc        <  2:299200/141‑1 (MQ=39)
                                                                                                                                         aTACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTg     >  1:424533/1‑141 (MQ=255)
                                                                                                                                            |                                                                                                                                            
GCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAGCCATTGCTACCTTCGTTGAAGGTGCCTGATC  >  CP000730/35875‑36155

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: