Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 36,087 A→G 62.4% C155C (TGT→TGC USA300HOU_0026 ← IS431mec transposase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP00073036,0870AG62.4% 707.8 / inf 908C155C (TGT→TGCUSA300HOU_0026IS431mec transposase
Reads supporting (aligned to +/- strand):  ref base A (190/152);  new base G (285/274);  total (478/430)
Fisher's exact test for biased strand distribution p-value = 1.92e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.88e-01

Only 100 of 910 total aligned reads displayed.

TTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAGCCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTAT  >  CP000730/35947‑36226
                                                                                                                                            |                                                                                                                                           
   ttATATTGGGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAg                                                                                                                                          <  2:894266/141‑1 (MQ=255)
    tctatcGAGCGTAAATACACTCAATACCTTTTAAATTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATCCAGATATTTCGATGTACAATGGCAGt                                                                                                                                         <  2:869981/139‑1 (MQ=14)
     atataGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGTGGTTATTCAGATATTTCTATTTACACTGGCAGTc                                                                                                                                        >  2:713753/1‑141 (MQ=14)
     atataGAGCGTAAATACACTCAATACCTTTTAAAGTAGTCTTTGCTGTATTGATCCTTTGATACCTTGTCTTTCTTACTTGAATATGACGGTTATCGTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTc                                                                                                                                        <  2:265044/141‑1 (MQ=14)
      tataGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCa                                                                                                                                       >  1:51025/1‑141 (MQ=255)
        taGAGCGTAAATACATTCAATACCTTTTAAAGGATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAgg                                                                                                                                     <  1:455841/141‑1 (MQ=255)
         agagCGTAAATACACTGAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGgt                                                                                                                                    >  1:539265/1‑141 (MQ=255)
          gagCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGGTATTTCGATGTACAATGGCAGTCAGgtt                                                                                                                                   >  1:59054/1‑141 (MQ=255)
          aagCGTAAACACATTCACTTCCTTTTAAAGTATTCTTTGCTGTATTGATNCTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGgtt                                                                                                                                   <  1:833013/140‑1 (MQ=255)
            gCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGgttta                                                                                                                                 <  1:519521/141‑1 (MQ=255)
              ttAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGATTATTCAGATATTTCGATGTACAATGCCAGTCAGgtttaag                                                                                                                               <  2:286448/140‑1 (MQ=255)
              gTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGgtttaag                                                                                                                               >  2:305700/1‑141 (MQ=255)
              gTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGgtttaag                                                                                                                               <  1:761260/141‑1 (MQ=255)
               tAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATATGATGCAATGTGGTTATTCAGATATTTCGATGTACAATGACAGTCAGgtttaagt                                                                                                                              >  1:855879/1‑141 (MQ=14)
               tAAATACACGCAATACCTTTTAAAGTATTCTTTGTTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGAGATTTCGATGTACAATGGCAGTCAGgtttaagt                                                                                                                              <  2:363326/141‑1 (MQ=14)
                aaaTACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTTAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGgtttaagtt                                                                                                                             <  2:765738/141‑1 (MQ=255)
                aaaTACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGgtttaagtt                                                                                                                             <  1:220743/141‑1 (MQ=255)
                 aaaacacTCAATACCTTTTAAAGTATTCTTTACTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATGTGGCTCAATGATGTTATTCAGATAGTTCGATGTACAATGTGTGTCaagtttaagttt                                                                                                                            >  2:233826/4‑141 (MQ=14)
                 aaTACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGgtttaagttt                                                                                                                            >  2:112694/1‑141 (MQ=40)
                 aaTACACTCAATACCTTTTAAAGTATTATTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGgtttaagttt                                                                                                                            <  2:637183/141‑1 (MQ=255)
                  aTACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGGTCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGgtttaagttta                                                                                                                           >  2:654266/1‑141 (MQ=255)
                  aTACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGgtttaagttta                                                                                                                           >  2:455876/1‑141 (MQ=255)
                  aTACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGgtttaagttta                                                                                                                           >  2:193648/1‑141 (MQ=255)
                   tACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGgtttaagtttaa                                                                                                                          >  1:329279/1‑141 (MQ=255)
                   tACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGgtttaagtttaa                                                                                                                          >  2:272869/1‑141 (MQ=255)
                   tACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAAGGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGgtttaagtttaa                                                                                                                          >  1:253000/1‑141 (MQ=255)
                    acacTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTaaa                                                                                                                         <  1:383135/141‑1 (MQ=255)
                     cactCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTaaaa                                                                                                                        >  2:85814/4‑141 (MQ=255)
                      actCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAg                                                                                                                       <  1:299195/139‑1 (MQ=255)
                      aTTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAg                                                                                                                       <  1:747938/141‑1 (MQ=40)
                        tCAATACCTTTTTAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCt                                                                                                                     <  2:515180/141‑1 (MQ=255)
                             aCCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATCCCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAAt                                                                                                                >  1:862107/1‑141 (MQ=255)
                              ccTTTTCAAGTATTCTTTGCTGTATTGATACTTTGATACCGTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAAtt                                                                                                               <  1:81424/141‑1 (MQ=255)
                              ccTTTTAAAGTATTCTTTGCCGTATTGATACTTTGCTCTCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAAtt                                                                                                               >  2:150852/1‑141 (MQ=255)
                               cTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTa                                                                                                              >  2:802603/1‑141 (MQ=255)
                               cTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGGTTAAGTTTAAAAGCTTTAATTa                                                                                                              >  1:895545/1‑141 (MQ=255)
                               cTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAGttg                                                                                                              >  1:178068/1‑140 (MQ=255)
                                      aGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTaac                                                                                                       >  1:102536/1‑139 (MQ=255)
                                       gTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTaacc                                                                                                      <  2:326134/141‑4 (MQ=255)
                                         aTTCTTTTCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCAt                                                                                                    <  2:846265/141‑1 (MQ=255)
                                          ttCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCAtt                                                                                                   <  2:539265/141‑1 (MQ=255)
                                          ttCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCAtt                                                                                                   <  2:397274/141‑1 (MQ=255)
                                             tttGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCt                                                                                                >  1:697347/1‑141 (MQ=255)
                                                gCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTAcc                                                                                             <  2:41739/141‑1 (MQ=255)
                                                 ccgTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCt                                                                                            <  1:347952/139‑1 (MQ=255)
                                                   gTATTGATCCTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGTTCTTGCACAATGAGGTTATTCAGATATTTCGATGTACCATGGCAGTCCGGTTTAAGTTTAAATGCTTTAATTACTTTAATCATTACTTCTTTc                                                                                          >  1:606315/1‑141 (MQ=14)
                                                     aTTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGt                                                                                        <  2:377517/141‑1 (MQ=255)
                                                     aTTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGt                                                                                        <  2:412363/141‑1 (MQ=255)
                                                     aTTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGAAGGTGATATTACTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCCGTCCGCTTTAAGTTGATAATCTTTAATTACTTTAACCATTGCTACCTTCGt                                                                                        >  2:319457/1‑141 (MQ=14)
                                                          tACTTTTATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAg                                                                                   <  1:194590/141‑1 (MQ=255)
                                                          tACTTTGATACCTTTTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAg                                                                                   <  1:719577/141‑1 (MQ=255)
                                                            cTTTGATCCCTCGTCTTTGTTACTTTAATATGACGGTGATCTTGCTGAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTTCTTTAACCATTGCTACCTTCGTTGAAGGt                                                                                 <  2:813566/141‑1 (MQ=11)
                                                            cTTTGATAGCTTGTCTGTCTTACTTTAATATGACGGTTATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGt                                                                                 <  1:365622/141‑1 (MQ=14)
                                                             tttGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTGAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTg                                                                                >  2:550682/1‑141 (MQ=255)
                                                               tGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGcc                                                                              >  1:795437/1‑141 (MQ=255)
                                                                 aTACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTACTTACTTTAACACTTGCTACTTGGCTTGAAGGTGCCTg                                                                            >  2:747370/1‑141 (MQ=14)
                                                                 aTACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTg                                                                            >  1:424533/1‑141 (MQ=255)
                                                                      ttGTCTTTCTTATTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTg                                                                       <  2:340235/141‑1 (MQ=255)
                                                                            ttcttACTTTAATATGACGGTGATCTTGGTCAATGGGGTTATTTAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTaactt                                                                 >  2:784291/1‑138 (MQ=11)
                                                                              cttACTTTCATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTAcc                                                               <  1:887029/141‑1 (MQ=11)
                                                                              cttACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTAcc                                                               >  1:672093/1‑141 (MQ=255)
                                                                                 aCTTTAATATGACGATGATCTTGCTCAATGAGGTTATTCAGATTTTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAGCCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCttt                                                            >  1:476359/1‑141 (MQ=25)
                                                                                  cTTTAATATGACGGTGATCTTGCTGAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCtttt                                                           >  1:579114/1‑141 (MQ=255)
                                                                                  cTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCtttt                                                           <  1:404286/141‑1 (MQ=25)
                                                                                  cTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCtttt                                                           >  1:316704/1‑141 (MQ=255)
                                                                                   tttAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTg                                                          <  2:251842/141‑1 (MQ=25)
                                                                                      aaTATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAGCCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAgg                                                       >  1:269760/1‑141 (MQ=35)
                                                                                       atatGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTGTAGGt                                                      >  2:663500/1‑141 (MQ=255)
                                                                                          tGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTa                                                   >  1:140028/1‑141 (MQ=255)
                                                                                            aCGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTAcc                                                 <  2:496667/141‑1 (MQ=255)
                                                                                            aCGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTTAAAGCTTTAAGTATTTTAGACATTGTTGCTTTGGTTGATTGTGCCTGATCTGTAACTACCTTTTGTGGCTTTcc                                                 >  1:616586/1‑141 (MQ=11)
                                                                                             cGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCa                                                <  2:52701/141‑1 (MQ=18)
                                                                                             cGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCa                                                <  1:631769/141‑1 (MQ=11)
                                                                                              ggTTATCTTGCTCAATGATGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCaa                                               <  2:16922/141‑1 (MQ=11)
                                                                                               gTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTTAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCaaa                                              <  2:723570/141‑1 (MQ=12)
                                                                                               gTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCaaa                                              <  2:605808/141‑1 (MQ=12)
                                                                                                tGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTGTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAAt                                             >  2:826752/1‑141 (MQ=12)
                                                                                                tGATCTTGCTCAATGAGGTTATTCAGATATTGCGATGTACAATGGCCGTCAGTTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAAt                                             <  2:593443/141‑1 (MQ=11)
                                                                                                   tCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGt                                          >  2:623766/1‑141 (MQ=255)
                                                                                                      tGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTTCCAAATTGtttt                                       >  2:182482/1‑140 (MQ=12)
                                                                                                       gCTCAATGAGGTCATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTaa                                      <  1:28482/141‑1 (MQ=11)
                                                                                                       gCTCAATCAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCGTACCAAATTGTTTaa                                      >  1:567716/1‑141 (MQ=11)
                                                                                                            aTGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATgaga                                 >  2:355937/1‑141 (MQ=12)
                                                                                                             tGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGAc                                >  1:737577/1‑141 (MQ=17)
                                                                                                              gAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTATTGAGACg                               >  1:234659/1‑141 (MQ=12)
                                                                                                              gAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGAct                               >  1:915789/1‑140 (MQ=12)
                                                                                                                 gTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGttt                            <  2:434239/141‑1 (MQ=12)
                                                                                                                  tttgtCAGTTATTTCGCTGTATAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAAGTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACGACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTg                           <  2:124261/137‑1 (MQ=11)
                                                                                                                  ttgttCAGATATTTCGAGGTAAAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTAGCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTg                           <  2:207009/138‑1 (MQ=11)
                                                                                                                  ttATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTg                           >  1:123277/1‑141 (MQ=255)
                                                                                                                   tATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGa                          <  1:444170/141‑1 (MQ=17)
                                                                                                                   tATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGa                          <  1:157796/141‑1 (MQ=255)
                                                                                                                    aTTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGAt                         >  2:731494/1‑141 (MQ=12)
                                                                                                                       cAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATaaa                      >  2:573692/1‑141 (MQ=12)
                                                                                                                        aGATATTTCGATGTGCAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAAc                     >  1:495676/1‑141 (MQ=12)
                                                                                                                               tCGAGGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATg              <  2:913028/141‑1 (MQ=12)
                                                                                                                                  aTGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTg           >  2:475995/1‑141 (MQ=12)
                                                                                                                                      aCAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTaaaaa       >  2:677677/1‑139 (MQ=11)
                                                                                                                                        aaTGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGAt     <  1:645956/141‑1 (MQ=11)
                                                                                                                                           gACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTAt  <  2:696357/141‑1 (MQ=11)
                                                                                                                                            |                                                                                                                                           
TTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAGCCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTAT  >  CP000730/35947‑36226

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: