BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP000730	36,269	C→T	59.4%	A95T (GCA→ACA)	USA300HOU_0026 ←	IS431mec transposase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	36,269	0	C	T	59.4%	260.0 / inf	933	A95T (GCA→ACA)	USA300HOU_0026	IS431mec transposase
Reads supporting (aligned to +/- strand): ref base C (184/192); new base T (257/291); total (443/490)
Fisher's exact test for biased strand distribution p-value = 5.47e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.73e-01

Only 100 of 939 total aligned reads displayed.

TGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGT  >  CP000730/36129‑36409

   tgccTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATc                                                                                                                                           <  2:111847/139‑1 (MQ=255)

     ccTTCGTTGAAGGTACCTCATCTGTAATTACCTTTTAAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCaa                                                                                                                                         <  1:182482/141‑1 (MQ=12)

      cTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAAt                                                                                                                                        >  2:574783/1‑141 (MQ=17)

       ttCGTTGAAGTGGCCTTATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATg                                                                                                                                       <  2:249532/141‑1 (MQ=12)

             gAAGGTGCCTGATCTGTAATCACCTTTTGAGGTTTACCAAATTGTTGAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACACAACCCAATATCTAATGTATGTCCCTCCGCATCAATGGCACGa                                                                                                                                 >  1:641095/1‑141 (MQ=11)

             gAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGa                                                                                                                                 <  1:655228/141‑1 (MQ=255)

               aGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGata                                                                                                                               >  2:566586/1‑141 (MQ=17)

               aGGTGCCTGAGCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGata                                                                                                                               <  2:138387/141‑1 (MQ=17)

                ggTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACTCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATAAATGGCTAGatat                                                                                                                              >  1:627834/1‑141 (MQ=11)

                  tGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATaa                                                                                                                            <  1:359635/141‑1 (MQ=32)

                    ccTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAAt                                                                                                                          <  1:115151/141‑1 (MQ=17)

                      tGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCGTCTGTATCAATGACACGATATAAATAg                                                                                                                        >  2:166334/1‑141 (MQ=12)

                       gATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGc                                                                                                                       <  2:905385/141‑1 (MQ=12)

                        aTCGGTAACTGCCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTGGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCt                                                                                                                      <  2:674347/141‑1 (MQ=11)

                          cTGTAACTACCTTTTGAGGCTTCCCAAATTGTTTAATGATACGTTTGATACACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTcc                                                                                                                    <  1:489382/141‑1 (MQ=11)

                           tGTAATTCCCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATCTACATAGCGCCa                                                                                                                   <  2:557424/141‑1 (MQ=255)

                           tGTAACTACCTTTTGAGGCTTATCAAATTGTTTAATGAGACGTTAGCTTTATTGGTAGGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCa                                                                                                                   <  1:253229/141‑1 (MQ=11)

                            gTAATTACCTTTTGAGGTTTACCCAATTGTTTAATGAGACGGTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAGCCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCAt                                                                                                                  <  2:614710/141‑1 (MQ=255)

                            gTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAACTATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCAt                                                                                                                  <  1:602760/141‑1 (MQ=17)

                            gTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATGGCTCAAt                                                                                                                  >  2:187794/1‑141 (MQ=17)

                              aaTTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCAttt                                                                                                                >  1:841748/1‑141 (MQ=17)

                                ttACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGTTCGATTTTc                                                                                                              >  1:307881/1‑141 (MQ=12)

                                ctACCTTTTGGGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTc                                                                                                              <  2:348883/140‑1 (MQ=255)

                                 tACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCCttttgc                                                                                                             >  2:857627/1‑139 (MQ=17)

                                  aCCTTTTGAGGTTTATCAAATTGTTTAATGAGACGTTTGATAATCGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCt                                                                                                            <  1:53759/141‑1 (MQ=17)

                                   ccTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTGACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAACGGCACGATATAAATAGCTCCATTTTCCtt                                                                                                           <  1:158133/141‑1 (MQ=255)

                                    cTTTTTTGGTTTATCAATTTGTTTAATGATACGTTTTATTAACGCATTTGCTGATTGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCttt                                                                                                          <  1:233826/141‑1 (MQ=11)

                                      tttGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTACCGCTGTATCTATGGCACGCTATAAATAGATCAATTTTCCttttt                                                                                                        >  2:728309/1‑140 (MQ=11)

                                         gAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTAttt                                                                                                     >  1:904263/1‑141 (MQ=17)

                                          aggattcacAAATTGTTTAATGAGAAGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCGTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTAtttt                                                                                                    <  1:59333/133‑1 (MQ=11)

                                           ggTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATTTAATTTATGTCCCTCTGTATCAGTGGCACGATATAATTAGCTCCATTTTTCttttgttttg                                                                                                   >  1:784249/1‑141 (MQ=11)

                                                aCCAAAGGGTGTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCCCCATTTTCCTTTTATTTTGAtata                                                                                              <  1:467031/141‑1 (MQ=11)

                                                    aaTTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGtc                                                                                          >  2:895525/1‑141 (MQ=17)

                                                     aTTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGtct                                                                                         >  2:208291/1‑141 (MQ=17)

                                                       tGTTTAAGAAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCtca                                                                                       <  1:296305/141‑1 (MQ=255)

                                                        gcttAATGAGACGGTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCtcat                                                                                      <  1:861484/139‑1 (MQ=12)

                                                        gTTTAATGTGACTTTTTAAAAACCCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCAAGATATAAAGAGCTCCATTTTCCTTTTATTTTGATGTACGTCtcat                                                                                      <  2:149016/141‑1 (MQ=11)

                                                            tctGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAAt                                                                                  <  1:749344/139‑1 (MQ=12)

                                                             aTGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATa                                                                                 >  2:839488/1‑141 (MQ=17)

                                                              tGAGCCGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCGCGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATAc                                                                                <  1:812331/141‑1 (MQ=255)

                                                              tGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGATTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATAc                                                                                <  1:168857/141‑1 (MQ=12)

                                                                 gaCGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGcc                                                                             <  1:525290/141‑1 (MQ=17)

                                                                  aCGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCa                                                                            >  1:508326/1‑141 (MQ=17)

                                                                    gTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCGTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCAtt                                                                          <  2:302230/141‑1 (MQ=17)

                                                                      ttGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATGAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTg                                                                        <  1:708825/141‑1 (MQ=255)

                                                                      ttGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCGTCAATACGCCAttta                                                                        >  1:89018/1‑140 (MQ=12)

                                                                       tGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGt                                                                       >  2:70194/1‑141 (MQ=17)

                                                                        gATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATAACTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTGTTTTGCTATACGTCCCATCAATAccccttaggga                                                                      >  2:408142/1‑134 (MQ=11)

                                                                          tAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGtaat                                                                    <  2:443033/141‑1 (MQ=32)

                                                                           tacctcATTTGCTGAATGATTAGTTCGTTGCTTACGCAACCAAACATCTAATGTATGTCCCTCTGTATCAATGGCACTATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGtaata                                                                   <  2:316704/136‑1 (MQ=11)

                                                                           aaaCGCATATGCTTAATGATTATCTCTTTGCTTACGCAACGAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGtaata                                                                   <  2:33994/141‑1 (MQ=12)

                                                                             aCGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGTTGTACGTCTCATCAATACGCCATTTGtaataaa                                                                 >  1:216211/1‑140 (MQ=18)

                                                                             aCGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGtaattaa                                                                 >  1:776268/1‑138 (MQ=11)

                                                                                cATATGCTGACTGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCtt                                                              <  1:760634/141‑1 (MQ=255)

                                                                                cATATGCTGAAGGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCCCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCtt                                                              <  1:100644/141‑1 (MQ=255)

                                                                                  tatGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCtttt                                                            <  1:799844/141‑1 (MQ=35)

                                                                                  tatGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCtttt                                                            >  2:827325/1‑141 (MQ=34)

                                                                                    tGCTGAATGATTATCTCGTTGCTTACGCAAGCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCtttttt                                                          >  2:799730/1‑141 (MQ=255)

                                                                                      cTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTAt                                                        <  1:13424/141‑1 (MQ=255)

                                                                                      cTGAAGTATTATCTCGTTGCTGACGCAACCAAATATTTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTTTTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTAt                                                        <  1:336242/141‑1 (MQ=11)

                                                                                       ttaaTGATTATCTCTTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATg                                                       <  2:326876/139‑1 (MQ=255)

                                                                                       tGAATGATTAACTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGGACGTTATAAATAGCTACATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATg                                                       <  1:393525/141‑1 (MQ=255)

                                                                                         aaTGATTCGCTCTTTGCTTACGCAAACAAATATGTAATTTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTTCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCt                                                     <  2:479387/141‑1 (MQ=14)

                                                                                          aTGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCACTACGCAATTTGTAATAAGCGTTTTTATACtt                                                    >  2:774084/1‑141 (MQ=11)

                                                                                           tGAGTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCttt                                                   <  2:269760/141‑1 (MQ=255)

                                                                                            gATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCtttt                                                  >  1:869217/1‑141 (MQ=255)

                                                                                             aTTATCTCGTTGCTTACGCAACCAAATATCTAATTTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACTCCACTTTTATTATGCTTTTTCATGtttttt                                                 >  1:434653/1‑141 (MQ=11)

                                                                                             aTTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTATTAAGCTTTTTTATGCttttt                                                 >  1:373280/1‑141 (MQ=255)

                                                                                               tATCTCACTGCTCTCACAACCAAAGATCTAATGTATGTCACTCTGAATCAATGGCACGATATAAATAGCTACATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTttct                                               <  2:63463/141‑1 (MQ=14)

                                                                                                aTCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCCGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTttctt                                              >  2:533808/1‑141 (MQ=255)

                                                                                                aTCGCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTttctt                                              <  1:270581/141‑1 (MQ=255)

                                                                                                  ctcGTTGCTTACGCAACCAAATATCTAATGTATGTCCCGCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCGATACGCCAGTTGGGTTAATCTTTTTTATGCTTTCTCTTcc                                            <  1:670462/141‑1 (MQ=14)

                                                                                                   tcGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCa                                           <  1:9851/141‑1 (MQ=40)

                                                                                                        gCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTAATCAATACGTCATTTGTAACGGGCATTTTTCGGTTTTTTATTCCTAAttt                                      >  1:68997/1‑141 (MQ=11)

                                                                                                         cTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCGATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTg                                     <  2:587714/141‑1 (MQ=255)

                                                                                                         cTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCAATTTGTAATAAGATTTTTTATACTTTTTATTCCAAATTTg                                     >  2:463434/1‑141 (MQ=14)

                                                                                                          ttACGGAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGa                                    <  2:379448/141‑1 (MQ=255)

                                                                                                           tACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGAt                                   <  1:736987/141‑1 (MQ=40)

                                                                                                            aCGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTAATCAATACGCCATTTGTAATAAACTTTTGTATGCTTTTTCTTCCAAATTTgttt                                  >  1:406424/1‑138 (MQ=255)

                                                                                                             cGCTACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATACTCGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATat                                 <  1:349605/141‑2 (MQ=14)

                                                                                                               cAACCAAATATCTAAGGTATGTCCTCATGCATCGATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATataa                               <  2:226471/141‑4 (MQ=11)

                                                                                                                acccAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCACATTTGATATaaa                              <  2:514834/139‑1 (MQ=255)

                                                                                                                aaCCATATATCTAAGGGATCGCCCTCTCCACAATTGGCACGGTATAAATAGCGCCATTTTCCTTTTATTTTGCTGTACGTCTCACCCATACGTCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCACATTTGATATaaa                              <  2:807856/141‑1 (MQ=11)

                                                                                                                  ccAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCGTTTTCTTCCAAATTTGATATAAAAt                            <  2:881218/141‑1 (MQ=255)

                                                                                                                     aaTATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATACGCTTTTTTATGCTTTTTCTTCCAACTTTTATATAAAATTgg                         >  1:422273/1‑141 (MQ=255)

                                                                                                                     aaTATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTgg                         >  1:445480/1‑141 (MQ=38)

                                                                                                                      atatCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGTTATAAAATTggg                        >  1:827716/1‑141 (MQ=255)

                                                                                                                      atatCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTggg                        >  2:721042/1‑141 (MQ=38)

                                                                                                                       tatCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTgggg                       <  1:196133/141‑1 (MQ=255)

                                                                                                                       tatCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCCAATTTGATACAAAATTgggg                       <  1:549411/141‑1 (MQ=255)

                                                                                                                           tAATGTTTGTCTCTCTGTATCAATGGCACGTGATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAATCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCata                   <  2:432221/141‑1 (MQ=11)

                                                                                                                             atgtatgtCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATAtt                 <  2:497087/141‑1 (MQ=25)

                                                                                                                                  tgtCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTCTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTGCAAATGTTATATAAAATTGGGGCATATTCTTGa            <  2:602669/141‑1 (MQ=11)

                                                                                                                                     cccTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCATTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTTAAcc         >  2:746821/1‑141 (MQ=255)

                                                                                                                                      ccTCTGCATCAATGGCACGATATAAATCGGTCCACTTTCCTTTTATTTTGATGTACGTCTCATCAACACGCTATTCGTCATAAGCTTTTGTATCCTTTTTCTTCCATATTTGATATCAAATAGGGGCATATTCttacagca        >  2:68873/1‑135 (MQ=11)

                                                                                                                                      ccTCTGCATCAATGGCACGATATAAATATCCCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTGTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAAccc        <  1:319457/141‑1 (MQ=255)

                                                                                                                                        tctGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATACAATTGGGGCCGATTATTGAActaaa      >  1:578735/1‑137 (MQ=11)

                                                                                                                                        gctGTATCACTGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCaa      <  1:723263/140‑1 (MQ=255)

                                                                                                                                         ctGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAAc     >  2:396963/1‑141 (MQ=25)

                                                                                                                                          tGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACg    >  2:658897/1‑141 (MQ=25)

TGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGT  >  CP000730/36129‑36409

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`